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Anthocyanins are extensively studied for their health-related properties, including antibacterial activity against urinary tract infections (UTI). Among common fruits, blueberries, with their remarkable antioxidant capacity, are one of the richest sources. Anthocyanin-rich extracts were obtained from four varieties: Snowchaser, Star, Stella Blue and Cristina Blue, grown in the hot climate of Southern Spain. Their total anthocyanins contents (TAC) were determined spectrophotometrically, and the anthocyanin profile by ultra high performance liquid chromatography-tandem mass spectrometer (UHPLC-MS/MS). Their antioxidant activity was assessed by oxygen radical absorbance capacity (ORAC) assay, while antibacterial activity against strains isolated from UTI patients was assessed in vitro, helping to select the varieties with the highest bioactive potential. Star showed the highest TAC and antioxidant activity (1663 +/- 159 mg of cyanidin-3-O-glucoside (cy-3-O-glu) equivalents/100 g fresh weight (FW), 6345 +/- 601 mu mol Trolox equivalents (TE)/100 g FW, respectively), followed by Cristina Blue, Stella Blue and Snowchaser. As far as we know, this is the first time that cyanidin-3-rutinoside has been identified in blueberries. The extracts inhibited all the tested strains, MICs ranging from 0.4 mg/mL (for Stella Blue extract against UTIP. aeruginosa) to 9.5 mg/mL (for all extracts against UTIK. pneumoniaessp.pneumoniae). This is the first study that assessed in vitro the antibacterial activity of blueberries againstKlebsiella pneumoniae,Providencia stuartiiandMicrococcusspp. strains isolated from UTI.

Lately, the entire society's focus has turned towards consuming more natural food products and the production of chemicals obtained utilising green technologies. The use of chemicals as food additives is a concern for consumers. For this reason, the search for natural and more sustainable additives is a key step to control food risks while meeting consumers' requirements. Essential oils could be considered potential green food additives on the basis of their valuable properties (chemical and microbiological). Unfortunately, there is currently a lack of knowledge regarding the safety associated with the consumption of essential oils and/or their nano‐emulsions. In response to this issue, the Department of Nutrition and Food Science, Toxicology and Legal Medicine (DNFSTLM) from the Faculty of Pharmacy, Seville, Spain, in partnership with the University of Agricultural Sciences and Veterinary Medicine, Cluj‐Napoca, Romania, have engaged their efforts into the individual working programme titled ‘Risk assessment of food additives including dietary exposure’ as part of the European Food Risk Assessment Fellowship Programme (EU‐FORA). The individual programme was built on three modular pillars that were based on a ‘learning‐by‐doing’ approach. The fellow's activities focused on experimental toxicology and evaluation of the dietary intake for citrus essential oils nano‐emulsions (CEO‐NEs). The objective was to provide a foundation in toxicology and train the fellow with the skills necessary to conduct toxicological investigations for risk assessment. Acquiring expertise in applying the guidelines of the European Food Safety Authority (EFSA) and the Organization of Economic Cooperation and Development (OECD) was an important goal for the EU‐FORA experience. Furthermore, the fellow received training in dietary risk assessment and participated in experimental research on the chemical characterisation, digestion processes and mechanism of action of bioactive compounds. In light of the results obtained, the individual programme was beneficial, the fellow being successfully trained and integrated into the hosting team. Furthermore, this project set the stage for further collaboration.

The rising demand for natural and safe products has increased interest in essential oils (EOs) as alternatives to synthetic preservatives. EOs could be encapsulated in active packaging or incorporated in nano-emulsion systems and help extend food shelf life by inhibiting the growth of pathogens. H. officinalis and Agastache foeniculum (Lamiaceae) are widely used in food and beverages. This review aims to explores their potential food applications, focusing on their antimicrobial activities, chemical compositions, and toxicity. H. officinalis EO mainly consists of oxygenated monoterpenes (27.32–92.25%), with 1,8-cineole, isopinocamphone, and pinocamphone as key compounds. It also contains monoterpene hydrocarbons (3.84–67.24%), including β-pinene, β-phellandrene, and β-ocimene. A. foeniculum EO is rich in phenylpropanoids (22.39–84.67%), primarily estragole (3.2–94.89%) and methyl eugenol, along with oxygenated monoterpenes (0.08–54.51%), mainly menthone (31.58–34.3%). H. officinalis EO exhibited antimicrobial activity against Escherichia coli, Staphylococcus aureus, Bacillus cereus, Salmonella Typhimurium, Pseudomonas aeruginosa, and various fungi, including Penicillium, Cladosporium, Candida, and Aspergillus species. A. foeniculum EO seemed to be effective against fungi and Gram-positive bacteria but showed lower activity against Gram-negative bacteria. H. officinalis EO showed no mutagenic or genotoxic effects in the available studies, while the toxicity of A. foeniculum EO remains unstudied. H. officinalis EO exhibited potential preservative properties when added to ground meat or used as coating for cheese and shrimp. The results of this study provide critical insights into the possibilities of integrating these EOs into food preservation strategies and their potential contributions to enhancing food safety and sustainability.

Cylindrospermopsin (CYN) and microcystins (MC) are cyanotoxins that can occur simultaneously in contaminated water and food. CYN/MC-LR mixtures previously investigated in vitro showed an induction of micronucleus (MN) formation only in the presence of the metabolic fraction S9. When this is the case, the European Food Safety Authority recommends a follow up to in vivo testing. Thus, rats were orally exposed to 7.5 + 75, 23.7 + 237, and 75 + 750 μg CYN/MC-LR/kg body weight (b.w.). The MN test in bone marrow was performed, and the standard and modified comet assays were carried out to measure DNA strand breaks or oxidative DNA damage in stomach, liver, and blood cells. The results revealed an increase in MN formation in bone marrow, at all the assayed doses. However, no DNA strand breaks nor oxidative DNA damage were induced, as shown in the comet assays. The histopathological study indicated alterations only in the highest dose group. Liver was the target organ showing fatty degeneration and necrotic hepatocytes in centrilobular areas, as well as a light mononuclear inflammatory periportal infiltrate. Additionally, the stomach had flaking epithelium and mild necrosis of epithelial cells. Therefore, the combined exposure to cyanotoxins may induce genotoxic and histopathological damage in vivo.

The prevention and control of bacterial contamination on ready‐to‐eat (RTE) fresh produce is an essential task to ensure food safety. Therefore, the development of novel and effective decontamination technologies to ensure microbiological safety of fruits and vegetables has gained considerable attention and new sanitisation methods are needed. The antimicrobial activity of essential oils (EOs) is well documented, but their application in fresh produce remains a challenge due to their hydrophobic nature. Thus, nanoemulsions efficiently contribute to support the use of EOs in foods by enhancing their dispersibility, their contact area and facilitating the introduction into bacterial cells. The combination of these factors ultimately increases their antimicrobial activity. Quantitative microbial risk assessment (QMRA) is gaining more attention as an effective tool to assess and prevent potential risks associated with food‐borne pathogens. In this context, the current project aims to study the effectiveness of different washing methods based on nanoemulsified EOs, comparing them against traditional methods, using a QMRA model for Escherichia coli O157:H7 on cherry tomatoes. Different simulations within a stochastic risk assessment model were implemented using the biorisk package for R, aiming to describe microbial behaviour and biological risk along the Romanian and Spanish food supply chains of RTE fresh produce. Nanoemulsions were prepared using oregano and rosemary EOs, each from Romania and Spain. The four nanoemulsions were evaluated as decontamination treatments to control the growth of E. coli O157:H7 on artificially contaminated cherry tomatoes. The decontamination treatments showed encouraging results, comparable to commonly used chlorine solutions. Therefore, oregano and rosemary nanoemulsions are promising and could be a feasible alternative for chlorine solutions in the reduction of microbiological contaminants.

The agricultural sector is currently confronting multifaceted challenges such as an increased food demand, slow adoption of sustainable farming, a need for climate-resilient food systems, resource inequity, and the protection of small-scale farmers’ practices. These issues are integral to food security and environmental health. Remote sensing technologies can assist precision agriculture in effectively addressing these complex problems by providing farmers with high-resolution lenses. The use of vegetation indices (VIs) is an essential component of remote sensing, which combines the variability of spectral reflectance value (derived from remote sensing data) with the growth stage of crops. A wide array of VIs can be used to classify the crops and evaluate their state and health. However, precisely this high number leads to difficulty in selecting the best VI and their combination for specific objectives. Without thorough documentation and analysis of appropriate VIs, users might find it difficult to use remote sensing data or obtain results with very low accuracy. Thus, the objective of this review is to conduct a critical analysis of the existing state of the art on the effective use of VIs for the discrimination and monitoring of several important agricultural crops (wheat, corn, sunflower, soybean, rape, potatoes, and forage crops), grasslands and meadows. This data could be highly useful for all the stakeholders involved in agricultural activities. The current review has shown that VIs appear to be suitable for mapping and monitoring agricultural crops, forage crops, meadows and pastures. Sentinel-1 and Sentinel-2 data were the most utilized sources, while some of the frequently used VIs were EVI, LAI, NDVI, GNDVI, PSRI, and SAVI. In most of the studies, an array of VIs needed to be employed to achieve a good discrimination of crops or prediction of yields. The main challenges in using VIs are related to the variation of the spectral characteristics during the vegetation period and to the similarities of the spectral signatures of various crops and semi-natural meadows. Thus, further studies are needed to establish appropriate models for the use of satellite data that would prove to have greater accuracy and provide more relevant information for the efficient monitoring of agricultural crops.

Oranges are consumed globally, being widely appreciated for their nutritional benefits. Oranges embrace up to 53% of waste produced per year in the citrus industry, and most of the orange waste is destroyed because of its low nutritional value. But this problem could be overcome by extracting valuable compounds from orange waste and further using these compounds because of their bioactive nature. Orange essential oils are bioactive substances located mainly in the orange peel and have gained increased attention lately, being frequently utilized in the food, cosmetic, and pharmaceutical industries. Increased efforts have been made in finding efficient extraction methods of this valuable product. Novel effective, economic, and environmentally friendly extraction methods are needed for essential oil recovery, with minimal impact on the extracted yield and quality. Thus, this review critically assessed recent developments and improvements in the extraction of essential oil from orange waste by two innovative non-thermal methods: microwave and ultrasound-assisted extractions. The effectiveness in terms of cost, time, and energy consumption was assessed in comparison to conventional extraction methods (hydro-distillation, cold pressing, and steam distillation). The process parameters of the extraction methods and their effect on the yield and chemical composition of extracted essential oil were also studied, as this information could guide future efforts towards optimizing these technologies. Additionally, the challenges and future applications of microwave and ultrasound-assisted extractions techniques are also critically presented. Both microwave and ultrasound extractions showed improvements in terms of yield (11.5% vs 3.6%), cost, extraction time (30 min vs 180 min), carbon dioxide emission (160 g vs 2400 g), and energy saving (0.2 kW·h vs 3 kW·h) compared to conventional methods. However, there is a lack of implementation scenarios of these non-conventional techniques at industrial scale, as most studies were performed only at laboratory level. Thus, further research is needed for both microwave and ultrasound extractions to enable an effective transition toward large-scale implementation. Nonetheless, the main findings of this review are relevant to both research academics and industry professionals in research and development activities. This review offers critical insights that may advance applied research beyond the current state of the art by exploiting new approaches suitable for essential oil extraction. Additionally, it brings structured information to researchers supporting informed decision-making when selecting the most suitable essential oil extraction method for adding value from a by-product that is otherwise wasted.

The programme aimed at training the fellow in the risk assessment guidelines proposed by the EFSA in the field of contaminants, food contact materials, technological ingredients and nutritional risks. It had a modular ‘learning by doing’ approach and a balanced learning/case studies and theory. Module 1 offered an insight into chemical risk assessment and conferred transferable skills for a proper application of the framework. The hands‐on activities consisted of three case studies that went from a simple exercise on an official opinion, to working in a team with experts to produce a new opinion, to an individual work to obtain a publishable review manuscript. Module 2 was a training in experimental toxicology designed to create a toxicological basis and to enable the fellow to perform toxicological studies for risk assessment purposes. She joined the team working on cyanotoxins, gained experience with both EFSA and Organization of Economic Cooperation and Development (OECD) guidelines on genotoxicity and an insight into the developing of analytical methods suitable for risk assessment purposes. During module 3, the fellow was trained in nutritional risk assessment and involved in experimental work in chemical characterisation, biomarkers and mechanisms of action of bioactive compounds. This developed the critical perspective when assessing nutritional and health claims related the design of experiments, methods used, interpretation of results and human relevance. Module 4 provided a ‘hand‐on experience’ in scientific risk communication as the fellow was encouraged and supported in the participation at local, national and international workshops and congresses presenting the outcomes of the three modules. Thus, the fellow was successfully integrated in the day‐by‐day workflow of the department, gaining first‐hand practical experience in risk assessment in a multicultural and interdisciplinary context. This enabled a productive exchange of good practices and contributed to building a European risk assessment community.

Bacterial cellulose (BC) is a natural polymer with properties suitable for tissue engineering and possible applications in scaffold production. However, current procedures have limitations in obtaining BC pellicles with the desired structural, physical, and mechanical properties. Thus, this study analyzed the optimal culture conditions of BC membranes and two types of processing: draining and oven-drying. The aim was to obtain BC membranes with properties suitable for a wound dressing material. Two studies were carried out. In the preliminary study, the medium (100 mL) was inoculated with varying volumes (1, 2, 3, 4, and 5 mL) and incubated statically for different periods (3, 6, 9, 12, and 18 days), using a full factorial experimental design. Thickness, uniformity, weight, and yield were evaluated. In the optimization study, a Box–Behnken design was used. Two independent variables were used: inoculum volume (X1: 1, 3, and 5 mL) and fermentation period (X2: 6, 12, and 18 d) to determine the target response variables: thickness, swelling ratio, drug release, fiber diameter, tensile strength, and Young’s modulus for both dry and moist BC membranes. The mathematical modelling of the effect of the two independent variables was performed by response surface methodology (RSM). The obtained models were validated with new experimental values and confirmed for all tested properties, except Young’s modulus of oven-dried BC. Thus, the optimal properties in terms of a scaffold material of the moist BC were obtained with an inoculum volume of 5% (v/v) and 16 d of fermentation. While, for the oven-dried membranes, optimal properties were obtained with a 4% (v/v) and 14 d of fermentation.

The use of bacterial cellulose (BC) as scaffold for active biofilms is one of the most interesting applications, especially for the biomedical and food industries. However, there are currently few studies evaluating the potential of incorporating herbal extracts into various biomaterials, including BC. Thus, the aim of this study is to report a screening of the total phenolic content and antioxidant and antimicrobial activity of ethanolic extracts of oregano, rosemary, parsley, and lovage. At the same time, the bioactive potential of BC enriched with the four ethanolic extracts is described. Microwave-assisted extraction was used to extract bioactive compounds from the four selected herbs. The physical, mechanical, structural, and chemical properties of BC were also assessed. Next, BC was enriched with the extracts, and their effect against Escherichia coli, Staphylococcus aureus, and Candida albicans was evaluated. The results showed that the bioactivity of the herbs varied significantly, with rosemary extract being the most bioactive. The BC films possessed good mechanical properties, and a three-dimensional network fibrillar structure appropriate for ethanolic-extract incorporation. The BC samples enriched with rosemary extracts had the highest antibacterial activity against S. aureus, while E. coli. and C. albicans seemed to be resistant to all extracts, regardless of herbs.