Explore the vast range of titles available.

Background Rheumatoid arthritis (RA) is a chronic autoimmune disease marked by joint inflammation and destruction. Current treatments often have side effects and resistance. Cucurbitacin E (CuE), a natural compound with anti-inflammatory properties, shows therapeutic potential but its role in RA is unclear. This study explores CuE’s mechanisms in RA, focusing on M1 macrophage polarization. Methods We used a multi-omics approach, integrating transcriptomics, single-cell sequencing, and network pharmacology. Key steps included immune infiltration analysis, WGCNA, machine learning-based biomarker discovery, and flow cytometry validation. Pseudotime trajectory and cell communication analyses were also employed. Results Immune infiltration analysis revealed increased M1 macrophage infiltration in RA patients. WGCNA identified gene modules related to macrophage polarization. Machine learning identified five key biomarkers (CCR2, NFKB1, NT5E, PIK3R1, TYRO3). A diagnostic model based on these biomarkers achieved high accuracy (AUC = 0.94). Pseudotime and cell communication analyses suggested CuE may regulate M1 polarization and signaling networks. Flow cytometry showed high CuE concentrations inhibited M1 macrophage polarization. Conclusion CuE inhibits M1 macrophage polarization and related pathways, offering a promising RA treatment strategy. The identified biomarkers may serve as diagnostic and therapeutic targets. Future research should validate CuE’s clinical effects.

Objective This study aimed to evaluate the clinical outcomes and complications of open reduction and internal fixation (ORIF) combined with sartorius muscle iliac bone graft transplantation (SIBFT) in the treatment of displaced femoral neck fractures in middle-aged and young adults. Methods A retrospective analysis was performed on 26 patients under the age of 60 with displaced femoral neck fractures (Pauwels III or Garden III-IV) treated at our institution between April 2019 to July 2022. All patients underwent open reduction and internal fixation (ORIF) through the Smith-Petersen (S-P) approach, augmented with a sartorius muscle iliac bone graft transplantation.The fractures were secured with either three cannulated screws or three cannulated screws combined with a medial support steel plate. Postoperative follow-ups were scheduled at 1, 2, 3, 6, and 12 months to record bone healing time, complications, and assess Harris Hip Scores. Results Two patients were lost to follow-up, resulting in 24 patients who completed the follow-up with an average duration of 25.7 months. Bone healing was observed in 95.8% (23/24) of the patients, with a mean healing time of 5.0 months. Avascular necrosis of the femoral head occurred in 8.3% (2/24) of the patients after fracture healing. Harris Hip Score at the last follow-up was 89.75 (range 73–98). Conclusion Our preliminary results suggest that ORIF combined with sartorius muscle iliac bone graft transplantation for the treatment of displaced femoral neck fractures in middle-aged and young adults achieved satisfactory clinical outcomes.

Due to a lack of the realization mechanism and operational pattern of the urban-rural integration by land use, this study employs land use to regulate interface elements to achieve urban-rural integration development. Therefore, we analyzed urban-rural reality in Pukou District of Nanjing City, a typical metropolitan fringe of China, and investigated farmers’ willingness of typical representative villages. The results show that (1) According to the combination of resource environment, development intensity and development potential, Pukou District is divided into four land use areas, including optimization integration area, key development area, urban agricultural area, and ecotourism area. Most of the investigated farmers have a strong willingness to realize urban-rural integration by land use; (2) This study proposes an operational pattern of regional land use. The pattern is mainly based on “reality + willingness + policy” by using the three tools of “farmland reconsolidation, village reconstruction and factor reallocation”. It achieves urban-rural integration development through “zoning guidance–willingness driven–pattern selection–differentiated tools”.

This study presents the rough set theory and catastrophe progression method to regionalize the land-use change and to analyze the land ecological process. It uses the land-use database of Yixing City of Jiangsu Province, an urbanized and industrialized city in Yangtze River Delta of China, as an exemplification. The study area is divided into six kinds of land-use types according to the national standard of land-use classification. It includes arable land, garden, woodland, urban–rural construction land, water, and unused land. The six kinds of land-use types are formed into their corresponding landscape types in the scale of 1:10,000 by the aid of ArcGIS9.3 software of ESRI. In ArcGIS9.3, the landscape pattern indices are calculated by using Fragstats (raster version 3.3) software. Based on these landscape pattern indices, an integrated indicator system of landscape regionalization of land use in Yixing was established, and land-use regionalization models are set up using the catastrophe theory. Rough set theory is introduced to avoid the subjectivity in the indicator’s importance in catastrophe models. The hidden rule among the raw data is acquired by knowledge reduction of the data mining in the rough set theory. In the process, indicators needed to be arranged according to the computed importance of an attribute without considering the determination of weight function. This greatly avoids the subjectivity in the process of weight factor determination. The zoning of land use based on landscape indices finally is made by the multi-indicator integrated catastrophe progression method. According to these indices, Yixing is divided into four grading land-use zones when the rough set and catastrophe progression methods are combined. The zones include high-, medium-, low-, and weak-intensity zones, indicating that land use primarily varies the landscape pattern. With the increase of water and forest area proportion, the human disturbance to land system wanes; patch fragmentation reduces; patch shape complexity enhances; and landscape diversity decreases. Moreover, it can mostly avoid the subjective evaluation in artificially determining factor weights by using rough set theory. It makes the zoning results more objective and exact.

The Asian honeybee Apis cerana is one of two bee species that have been commercially kept with immense economic value. Here we present the analysis of genomic sequence and transcriptomic exploration for A. cerana as well as the comparative genomic analysis of the Asian honeybee and the European honeybee A. mellifera . The genome and RNA-seq data yield new insights into the behavioral and physiological resistance to the parasitic mite Varroa the evolution of antimicrobial peptides, and the genetic basis for labor division in A. cerana . Comparison of genes between the two sister species revealed genes specific to A. cerana , 54.5% of which have no homology to any known proteins. The observation that A. cerana displayed significantly more vigilant grooming behaviors to the presence of Varroa than A. mellifera in conjunction with gene expression analysis suggests that parasite-defensive grooming in A. cerana is likely triggered not only by exogenous stimuli through visual and olfactory detection of the parasite, but also by genetically endogenous processes that periodically activates a bout of grooming to remove the ectoparasite. This information provides a valuable platform to facilitate the traits unique to A. cerana as well as those shared with other social bees for health improvement.

Zhang, Z.; Liu, R.; Wang G.; Jia, Z.; Zhou, Y.; Cao, L., and Zhou, S., 2019. The construction of ecological network under the stress of road landscape in Dafeng, A coastal county in China. In: Lee, J.L.; Yoon, J.-S.; Cho, W.C.; Muin, M., and Lee, J. (eds.), The 3rd International Water Safety Symposium. Journal of Coastal Research, Special Issue No. 91, pp. 276-280. Coconut Creek (Florida), ISSN 0749-0208. In the process of rapid urbanization in coastal cities, the issue of landscape fragmentation, resulting from continuous expansion of artificial landscapes, has become increasingly prominent. Therefore, it is of great significance to build up ecological networks to connect fragmented habitats and protect biodiversity. From the perspective of impacts on natural landscapes of road system, the paper takes Dafeng, a coastal county in China, as a case study area, processes the selection of ecological sources, assignment and correction of resistance surface, and finally accomplishes the construction of ecological network, supported by techniques including GIS, Conefor Sensinode and so forth. The results demonstrates that the road network hinders the biological circulation between coastal wetland and interior landscape, resulting in the destruction of natural landscape integrity; with the modification of resistance surface based on the construction of road buffer, it can be observed that regional ecological resistance level increases significantly and the original long linear ecological corridors tend to be short with twists and turns; the wetland patches and corridor connecting them rank first in terms of the importance of landscape connectivity, which indicates that coastal management should be strengthened in avoidance of the aggravation of the landscape fragmentation under the stress of road landscape.

Recently, chelating agents have been widely applied to remove heavy metals for their highly effective capacities. In this study, three kinds of adsorption materials, 719 strongly basic anion exchange resin, [gamma]-Al2O3 and activated carbon, were investigated to remove copper(II) under the existence of chelating agent EDDS. Adsorption isotherm and kinetics of copper(II) were assessed in batch experimental systems, pH and initial concentrations were also conducted to evaluate the effects on copper(II) removal. Results indicated the adsorption capacity with three absorbents was enhanced from 0.62, 7.26, 6.73 mg/g to 17.21, 24.68, 24.90 mg/g respectively when the chelating agent EDDS existing in aquatic phase. The experimental isotherms are fitted to Langmuir and Freundlich models, results show that 719 strongly basic anion exchange resin and [gamma]-Al2O3 have good correlation coefficients obtained from Langmuir model, and activated carbon is better to fit the Freundlich model.