Explore the vast range of titles available.

We describe in this paper the experimental procedure, the data treatment and the quantification of the black body correction: an experimental approach to compensate for scattering and systematic biases in quantitative neutron imaging based on experimental data. The correction algorithm is based on two steps; estimation of the scattering component and correction using an enhanced normalization formula. The method incorporates correction terms into the image normalization procedure, which usually only includes open beam and dark current images (open beam correction). Our aim is to show its efficiency and reproducibility: we detail the data treatment procedures and quantitatively investigate the effect of the correction. Its implementation is included within the open source CT reconstruction software MuhRec. The performance of the proposed algorithm is demonstrated using simulated and experimental CT datasets acquired at the ICON and NEUTRA beamlines at the Paul Scherrer Institut.

Within neutron imaging, different methods have been developed with the aim to go beyond the conventional contrast modalities, such as grating interferometry. Existing grating interferometers are sensitive to scattering in a single direction only, and thus investigations of anisotropic scattering structures imply the need for a circular scan of either the sample or the gratings. Here we propose an approach that allows assessment of anisotropic scattering in a single acquisition mode and to broaden the range of the investigation with respect to the probed correlation lengths. This is achieved by a far-field grating interferometer with a tailored 2D-design. The combination of a directional neutron dark-field imaging approach with a scan of the sample to detector distance yields to the characterization of the local 2D real-space correlation functions of a strongly oriented sample analogous to conventional small-angle scattering. Our results usher in quantitative and spatially resolved investigations of anisotropic and strongly oriented systems beyond current capabilities. Acquiring orientation-resolved neutron images currently requires the sample or system to be rotated, precluding single-shot measurement. Here, the authors achieve small-angle scattering information with spatial resolution in a single shot through the inclusion of a circular diffraction grating.

A crucial criterion for the quality of the additively manufactured parts is the porosity content for achieving an acceptable final relative density. In addition, for jewelry applications, visible pores are unacceptable at or in the vicinity of the surface. In this study, non-destructive 3D neutron microtomography is applied to map the spatial distribution of pores in additively manufactured red-gold samples. The 3D imaging assessment underlines the high relative density of the printed red-gold sample and indicates residual pore sizes are predominantly below the limit of concern for jewelry applications. The 3D maps of pores within printed samples highlight the effect of the scanning strategy on the final quality and location of pores in the printed samples. These results confirm that neutron microtomography is a novel and precise tool to characterize residual porosity in additively manufactured gold alloys and other higher-Z materials where such investigation using other non-destructive methods (such as X-rays) is challenging due to the limited penetration depth.

Cortical force generators connect epithelial polarity sites with astral microtubules, allowing dynein movement to orient the mitotic spindle as astral microtubules depolymerize. Complexes of the LGN and NuMA proteins, fundamental components of force generators, are recruited to the cortex by Gαi-subunits of heterotrimeric G-proteins. They associate with dynein/dynactin and activate the motor activity pulling on astral microtubules. The architecture of cortical force generators is unknown. Here we report the crystal structure of NuMA:LGN hetero-hexamers, and unveil their role in promoting the assembly of active cortical dynein/dynactin motors that are required in orchestrating oriented divisions in polarized cells. Our work elucidates the basis for the structural organization of essential spindle orientation motors. LGN and NuMA link epithelial polarity sites with astral microtubules and associate with dynein, but the architecture of such cortical force-generating complexes is unknown. Here, the authors report the crystal structure of NuMA:LGN hetero-hexamers, and unveil their role in promoting the assembly of active cortical dynein/dynactin motors.

The ASPECT-BET project, or An sdd-SPECTrometer for BETa decay studies, aims to develop a novel technique for the precise measurement of forbidden beta spectra in the 10 keV–1 MeV range. This technique employs a Silicon Drift Detector (SDD) as the main spectrometer with the option of a veto system to reject events exhibiting only partial energy deposition in the SDD. A precise understanding of the spectrometer’s response to electrons is crucial for accurately reconstructing the theoretical shape of the beta spectrum. To compute this response, GEANT4 simulations optimized for low-energy electron interactions are used and validated with a custom-made electron gun. In this article we present the performance of these simulations in reconstructing the electron spectra measured with SDDs of a 109Cd monochromatic source, both in vacuum and in air. The allowed beta spectrum of a 14C source was also measured and analyzed, proving that this system is suitable for the application in ASPECT-BET.

Purpose Several studies highlighted a correlation between folic acid deficiency and high plasma homocysteine concentration, considered a risk factor for multifactorial diseases. Natural folates represent an emerging alternative strategy to supplementation with synthetic folic acid, whose effects are controversial. The present work was, therefore, performed in hyperhomocysteinemic mice to study the impact of supplementation with dairy matrices containing natural folates on plasma homocysteine levels and faecal microbiota composition. Methods Forty mice were divided into six groups, two of which fed control or folic acid deficient (FD) diets for 10 weeks. The remaining four groups were fed FD diet for the first 5 weeks and then shifted to a standard control diet containing synthetic folic acid (R) or a FD diet supplemented with folate-enriched fermented milk (FFM) produced by selected lactic acid bacteria, fermented milk (FM), or milk (M), for additional 5 weeks. Results Supplementation with dairy matrices restored homocysteine levels in FD mice, although impacting differently on hepatic S -adenosyl-methionine levels. In particular, FFM restored both homocysteine and S -adenosyl-methionine levels to the control conditions, in comparison with FM and M. Next generation sequencing analysis revealed that faecal microbiota of mice supplemented with FFM, FM and M were characterised by a higher richness of bacterial species in comparison with C, FD and R groups. Analysis of beta diversity highlighted that the three dairy matrices determined specific, significant variations of faecal microbiota composition, while hyperhomocysteinemia was not associated with significant changes. Conclusions Overall, the results represent a promising starting point for the applicability of food matrices enriched in natural folates to manage hyperhomocysteinemia.

Thrombospondin (TSP)-1 and TSP-2 share similar structures and functions, including a remarkable antiangiogenic activity. We have previously demonstrated that a mechanism of the antiangiogenic activity of TSP-1 is the interaction of its type III repeats domain with fibroblast growth factor-2 (FGF2), affecting the growth factor bioavailability and angiogenic activity. Since the type III repeats domain is conserved in TSP-2, this study aimed at investigating whether also TSP-2 retained the ability to interact with FGF2. The FGF2 binding properties of TSP-1 and TSP-2 and their recombinant domains were analyzed by solid-phase binding and surface plasmon resonance assays. TSP-2 bound FGF2 with high affinity (Kd = 1.3 nM). TSP-2/FGF2 binding was inhibited by calcium and heparin. The FGF2-binding domain of TSP-2 was located in the type III repeats and the minimal interacting sequence was identified as the GVTDEKD peptide in repeat 3C, corresponding to KIPDDRD, the active sequence of TSP-1. A second putative FGF2 binding sequence was also identified in repeat 11C of both TSPs. Computational docking analysis predicted that both the TSP-2 and TSP-1-derived heptapeptides interacted with FGF2 with comparable binding properties. Accordingly, small molecules based on the TSP-1 active sequence blocked TSP-2/FGF2 interaction. Binding of TSP-2 to FGF2 impaired the growth factor ability to interact with its cellular receptors, since TSP-2-derived fragments prevented the binding of FGF2 to both heparin (used as a structural analog of heparan sulfate proteoglycans) and FGFR-1. These findings identify TSP-2 as a new FGF2 ligand that shares with TSP-1 the same molecular requirements for interaction with the growth factor and a comparable capacity to block FGF2 interaction with proangiogenic receptors. These features likely contribute to TSP-2 antiangiogenic and antineoplastic activity, providing the rationale for future therapeutic applications.

Mesenchymal stromal cells (MSCs) are emerging as a novel therapeutic option for limiting chronic kidney disease progression. Conditioned medium (CM) containing bioactive compounds could convey similar benefits, avoiding the potential risks of cell therapy. This study compared the efficacy of nonrenal and renal cell-based therapy with the corresponding CM in rats with renal mass reduction (RMR). Infusions of human kidney stromal cells (kPSCs) and CM-kPSCs, but not umbilical cord (uc) MSCs or CM-ucMSCs, reduced proteinuria and preserved podocyte number and nephrin expression in RMR rats. Glomerular fibrosis, microvascular rarefaction, and apoptosis were reduced by all treatments, while the peritubular microvascular loss was reduced by kPSCs and CM-kPSCs treatment only. Importantly, kPSCs and CM-kPSCs reduced NG2-positive pericytes, and all therapies reduced α-smooth muscle actin expression, indicating reduced myofibroblast expansion. Treatment with kPSCs also significantly inhibited the accumulation of ED1-positive macrophages in the renal interstitium of RMR rats. These findings demonstrate that the CM of ucMSCs and kPSCs confers similar renoprotection as the cells. kPSCs and CM-kPSCs may be superior in attenuating chronic renal injury as a cell source.

Low-Angle Normal Faults (LANFs) represent in the central Southern Alps area (N Italy) the main structures along which the Variscan basement is in contact with the Upper Carboniferous-Permian volcanic-sedimentary succession. Tourmalinites frequently occur along LANFs, usually replacing former cataclasites. The mineralogy and chemical composition of tourmalinites point to a metasomatic origin. LANFs, together with high-angle faults, controlled the opening of the Permian Orobic Basin and likely acted as a preferred pathway for hydrothermal fluids that triggered the Boron-metasomatism. Along the Aga-Vedello LANF, tourmalinites appear to have formed after the cessation of fault activity, as no brittle post-metasomatism deformation overprint has been observed. These relationships suggest that the circulation of B-rich fluids occurred after the opening of the Orobic Basin that is broadly constrained to the Early Permian. At the same time, ca. 285–270 Ma, a strong magmatic activity affected all the Southern Alps, ranging in composition from mafic to acidic rocks and from intrusions at deep crustal levels to effusive volcanic products. The Early Permian magmatism was likely the source of the late-stage hydrothermal fluids that formed the tourmalinites. The same fluids could also have played a significant role in the formation of the Uranium ore deposit of the Novazza-Vedello mining district, as the ore bodies in the Vedello valley are concentrated along the basement-cover contact.

We present the status of the ASPECT-BET (An sdd-SPECTrometer for BETa decay studies) project which is aimed at developing a new detection strategy to perform high-precision, high-accuracy measurements of the energy spectra of beta decays of interest for the physics community, in particular in the field of nuclear physics, double beta decay and reactor neutrinos. The aim is to exploit a relatively novel spectroscopic technique based on Silicon Drift Detectors. An SDD-spectrometer, equipped with all the ancillary detectors required to reject events with only a partial energy deposition in the main sensitive elements, will provide high-statistics and virtually zero-background data. In order to isolate and study the systematic uncertainties, the statistical error on the measured spectra has to be reduced to a negligible level, balancing source activity, measurement duration and background. Reliable and well understood Montecarlo simulations are a key component of this application, as they provide a model for the response functions of the spectrometer, to be deconvolved from the data in order to correctly reconstruct the original spectral shapes. Thanks to the flexibility of the SDD detector technology, the here presented spectrometer could be coupled to a variety of beta sources, ranging from nuclei deposited on the surface of SDDs to minimise source self-absorpion to short-lived isotopes created and collected at unstable isotope beams like ISOLDE at CERN or the exotic beams at LNS, Catania. The current status of the technology, as well as some preliminary sensitivity studies, are presented and discussed.