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While the signaling pathways and transcription factors involved in the differentiation of thyroid follicular cells, both in embryonic and adult life, are increasingly well understood, the underlying mechanisms and potential crosstalk between the thyroid transcription factors Nkx2.1, Foxe1 and Pax8 and inductive signals remain unclear. Here, we focused on the transcription factor Sox9, which is expressed in Nkx2.1-positive embryonic thyroid precursor cells and is maintained from embryonic development to adulthood, but its function and control are unknown. We show that two of the main signals regulating thyroid differentiation, TSH and TGFβ, modulate Sox9 expression. Specifically, TSH stimulates the cAMP/PKA pathway to transcriptionally upregulate Sox9 mRNA and protein expression, a mechanism that is mediated by the binding of CREB to a CRE site within the Sox9 promoter. Contrastingly, TGFβ signals through Smad proteins to inhibit TSH-induced Sox9 transcription. Our data also reveal that Sox9 transcription is regulated by the thyroid transcription factors, particularly Pax8. Interestingly, Sox9 significantly increased the transcriptional activation of Pax8 and Foxe1 promoters and, consequently, their expression, but had no effect on Nkx2.1 . Our study establishes the involvement of Sox9 in thyroid follicular cell differentiation and broadens our understanding of transcription factor regulation of thyroid function.

El presente artículo tiene como objetivo explorar sociológicamente cómo operan aquellas estrategias gerenciales que promueven y normalizan la constante sobreexposición pública de los trabajadores. Nos centraremos en el sector de la consultoría, tanto por su influencia para generar tendencias organizacionales globales como por su capacidad para reflejar las transformaciones que se están dando en el mundo laboral. Para ello, hemos desarrollado un trabajo cualitativo compuesto por una autoetnografía en dos empresas del sector y una serie de entrevistas a consultores. Las estrategias de sobreexposición analizadas en este texto son expresión de lógicas de funcionamiento del poder que condicionan la experiencia de los trabajadores, contribuyendo a consolidar formas de subjetivación individualistas prototípicas del contexto neoliberal contemporáneo. Se basan en una autonomía paradójica y lógicas de presión y competición horizontal que operan desde la visibilidad y las retóricas meritocráticas

PurposeIn this article, the authors explore the emotional dimension of the strategies that researchers carry out to foster collaboration within research groups in science.Design/methodology/approachThe authors have carried out fieldwork based on semi-structured interviews with members of high-performance research groups, supported by the analysis of ethnographic observations in the local work contexts of several of these groups.FindingsAs a result, the authors start from the representations of the principal investigators interviewed about collaboration, as a dynamic between the individuals involved and the group, to describe two forms of leadership strategies: ego-centered or group. Hence, the authors highlight that the emotional work of IPs consists of combining both strategies by activating and deactivating affection, warmth and spontaneity in interactions. The authors conclude by reviewing the contributions and some potential lines of study.Research limitations/implicationsThe main contributions and conclusions must be regarded considering several limitations of our work. First, the authors have focused on high-performance groups, so it would be expected that the research groups that do not have the support of European Research Council (ERC) do not present the observed dynamics. Concerning the analysis, the authors have focused on the link between situational (personal interaction) and organizational scales. Although the authors have attended social factors that imply the structure and changes of the professional sector of science (Whitley, 2000, 2014), future publications will allow the authors to delve into relationships on a broader scale, associating collaboration patterns and discursive positions. The authors will focus on (1) roles and social profiles and (2) features of scientific culture and its recent configuration.Practical implicationsThe authors affirm that the emotional field is key to understanding how groups and individuals respond to these profound changes. In their work, scientific professionals do not only act based on rational and instrumental criteria but also driven by habits, affective networks and inertia of their organizational cultures. In this paper, the authors provide theoretical and practical keys to understand the complexity that collaboration creates within research groups as a contradictory reality that consists of a constant movement of individuals and the group.Social implicationsAs many of the interviewees expressed, there are no adequate spaces to learn and reflect on leadership and collaboration in science. The authors are science workers who are responsible for imagining and deciding how the authors want their research groups to function. Institutions must ensure the means so that this task can be carried out. Hopefully, this article will contribute to this irrevocable project.Originality/valueThe authors’ aim is to understand how well-known groups operate in their field, with a high level of resources and productivity, in order to identify and promote cutting-edge strategies in different scientific branches. Moreover, the authors want to recognize the importance of institutional infrastructures. For this reason, giving them a place in the framework of management studies allows to submit this issue to public debate for a wide audience of social scientists. Moreover, this permits to inquire about cross-disciplinary subjects, such as social and organizational psychology, sociology of work, studies about science and management studies.

Cancer-derived small extracellular vesicles (sEVs) are capable of modifying the tumor microenvironment and promoting tumor progression. Ovarian cancer (OvCa) is a lethal malignancy that preferentially spreads through the abdominal cavity. Thus, the secretion of such vesicles into the peritoneal fluid could be a determinant factor in the dissemination and behavior of this disease. We designed a prospective observational study to assess the impact of peritoneal fluid-derived sEVs (PFD-sEVs) in OvCa clinical outcome. For this purpose, 2 patient cohorts were enrolled: patients with OvCa who underwent a diagnostic or cytoreductive surgery and nononcological patients, who underwent abdominal surgery for benign gynecological conditions and acted as the control group. Systematic extraction of PFD-sEVs from surgical samples enabled us to observe significant quantitative and qualitative differences associated with cancer diagnosis, disease stage, and platinum chemosensitivity. Proteomic profiling of PFD-sEVs led to the identification of molecular pathways and proteins of interest and to the biological validation of S100A4 and STX5. In addition, unsupervised analysis of PFD-sEV proteomic profiles in high-grade serous ovarian carcinomas (HGSOCs) revealed 2 clusters with different outcomes in terms of overall survival. In conclusion, comprehensive characterization of PFD-sEV content provided a prognostic value with potential implications in HGSOC clinical management.

In eukaryotes, the decapping machinery is highly conserved and plays an essential role in controlling mRNA stability, a key step in the regulation of gene expression. Yet, the role of mRNA decapping in shaping gene expression profiles in response to environmental cues and the operating molecular mechanisms are poorly understood. Here, we provide genetic and molecular evidence that a component of the decapping machinery, the LSM1-7 complex, plays a critical role in plant tolerance to abiotic stresses. Our results demonstrate that, depending on the stress, the complex from Arabidopsis thaliana interacts with different selected stress-inducible transcripts targeting them for decapping and subsequent degradation. This interaction ensures the correct turnover of the target transcripts and, consequently, the appropriate patterns of downstream stress-responsive gene expression that are required for plant adaptation. Remarkably, among the selected target transcripts of the LSM1-7 complex are those encoding NCED3 and NCED5, two key enzymes in abscisic acid (ABA) biosynthesis. We demonstrate that the complex modulates ABA levels in Arabidopsis exposed to cold and high salt by differentially controlling NCED3 and NCED5 mRNA turnover, which represents a new layer of regulation in ABA biosynthesis in response to abiotic stress. Our findings uncover an unanticipated functional plasticity of the mRNA decapping machinery to modulate the relationship between plants and their environment.

Wetlands are transitional lands between terrestrial and aquatic systems that provide various ecosystem services. The objective of this study was to evaluate the change in wetlands in the Chimborazo Wildlife Reserve (CR) in the period 1986–2022 using geographic information systems (GISs), multitemporal satellite data, and field data from the 16 wetlands of the reserve. Images from Landsat satellite collections (five from Thematic Mapper, seven from Enhanced Thematic Mapper, and eight from Operational Land Imager and Thermal Infrared Sensor) were used. Image analysis and processing was performed, and the resulting maps were evaluated in a GIS environment to determine the land cover change and growth rate of hydrophilic opportunistic vegetation (HOV) according to hillside orientation. The results show that there are negative annual anomalies in the water-covered areas, which coincide with the increase in HOV. This shows that the constancy or increase in the rate of increase in HOV, which varies between 0.0018 and 0.0028, causes the disappearance of these ecosystems. The importance of the study lies in its potential contribution to the decision-making process in the management of the CR.