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"Castillo, Ana"
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Sustainable Tourism as a Driving force of the Tourism Industry in a Post-Covid-19 Scenario
The tourism industry is probably one of the most affected by the crisis caused by Covid-19. It is the responsibility of politicians, tourism professionals and researchers to look for solutions to revive this important industry. This article shows how the development of Sustainable Tourism can help in the sustenance of the tourism industry, since one of the premises on which Sustainable Tourism is based is the non-overcrowding of tourist destinations (essential factor in the current context). Considering this argument and the existing regulations on lockdown rules, social distancing and meet up, it is considered that the practices in Sustainable Tourism can become a potential solution to stimulate tourist movements and help the revival of the tourism industry. Therefore, more specifically, the main objective of this article is to know tourist´s perception among about Sustainable Tourism and to determine which factors help its development. In this sense, the use of structural equation models in a research of 308 tourists has determined how factors related to the tourists’ attitude, motivation and perceived benefits provided by the development of Sustainable Tourism increase the intention to consume this type of tourism.
Journal Article
Mitochondrial Fusion and ERK Activity Regulate Steroidogenic Acute Regulatory Protein Localization in Mitochondria
The rate-limiting step in the biosynthesis of steroid hormones, known as the transfer of cholesterol from the outer to the inner mitochondrial membrane, is facilitated by StAR, the Steroidogenic Acute Regulatory protein. We have described that mitochondrial ERK1/2 phosphorylates StAR and that mitochondrial fusion, through the up-regulation of a fusion protein Mitofusin 2, is essential during steroidogenesis. Here, we demonstrate that mitochondrial StAR together with mitochondrial active ERK and PKA are necessary for maximal steroid production. Phosphorylation of StAR by ERK is required for the maintenance of this protein in mitochondria, observed by means of over-expression of a StAR variant lacking the ERK phosphorylation residue. Mitochondrial fusion regulates StAR levels in mitochondria after hormone stimulation. In this study, Mitofusin 2 knockdown and mitochondrial fusion inhibition in MA-10 Leydig cells diminished StAR mRNA levels and concomitantly mitochondrial StAR protein. Together our results unveil the requirement of mitochondrial fusion in the regulation of the localization and mRNA abundance of StAR. We here establish the relevance of mitochondrial phosphorylation events in the correct localization of this key protein to exert its action in specialized cells. These discoveries highlight the importance of mitochondrial fusion and ERK phosphorylation in cholesterol transport by means of directing StAR to the outer mitochondrial membrane to achieve a large number of steroid molecules per unit of StAR.
Journal Article
Sustainable Tourism as a Source of Healthy Tourism
Even though the World Tourism Organization described Sustainable Tourism as a tourism form that could contribute to the future survival of the industry, the current reality is quite different, since it has not been firmly established in society at expected levels. The present study analyzes which variables drive the consumption of this tourism type, taking tourist awareness as the key element. To this awareness, we must add the current crisis experienced by the tourism industry caused by COVID-19, since it can benefit Sustainable Tourism development, promoting less crowded destinations that favor social distancing. For this, the existing literature on Sustainable Tourism has been examined in order to create a model that highlights the relations among these variables. To determine the meaning of these relations, a sample of 308 tourists was analyzed through structural equation models using Partial Least Squares. The results show that there is a clear attitude on the part of the tourist to develop Sustainable Tourism, driven by the positive effects and motivation it entails, as well as the satisfaction the tourist perceives when consuming a responsible tourism type.
Journal Article
New inhibitor targeting Acyl-CoA synthetase 4 reduces breast and prostate tumor growth, therapeutic resistance and steroidogenesis
Acyl-CoA synthetase 4 (ACSL4) is an isoenzyme of the fatty acid ligase-coenzyme-A family taking part in arachidonic acid metabolism and steroidogenesis. ACSL4 is involved in the development of tumor aggressiveness in breast and prostate tumors through the regulation of various signal transduction pathways. Here, a bioinformatics analysis shows that the ACSL4 gene expression and proteomic signatures obtained using a cell model was also observed in tumor samples from breast and cancer patients. A well-validated ACSL4 inhibitor, however, has not been reported hindering the full exploration of this promising target and its therapeutic application on cancer and steroidogenesis inhibition. In this study, ACSL4 inhibitor PRGL493 was identified using a homology model for ACSL4 and docking based virtual screening. PRGL493 was then chemically characterized through nuclear magnetic resonance and mass spectroscopy. The inhibitory activity was demonstrated through the inhibition of arachidonic acid transformation into arachidonoyl-CoA using the recombinant enzyme and cellular models. The compound blocked cell proliferation and tumor growth in both breast and prostate cellular and animal models and sensitized tumor cells to chemotherapeutic and hormonal treatment. Moreover, PGRL493 inhibited de novo steroid synthesis in testis and adrenal cells, in a mouse model and in prostate tumor cells. This work provides proof of concept for the potential application of PGRL493 in clinical practice. Also, these findings may prove key to therapies aiming at the control of tumor growth and drug resistance in tumors which express ACSL4 and depend on steroid synthesis.
Journal Article
An efficient transformation method for genome editing of elite bread wheat cultivars
An efficient genetic transformation protocol is necessary to edit genes for trait improvement directly in elite bread wheat cultivars. We used a protein fusion between a wheat growth-regulating factor 4 (GRF4) and its interacting factor (GIF1) to develop a reproducible genetic transformation and regeneration protocol, which we then used to successfully transform elite bread wheat cultivars Baj, Kachu, Morocco, Reedling, RL6077, and Sujata in addition to the experimental cultivar Fielder. Immature embryos were transformed with the vector using particle bombardment method. Transformation frequency increased nearly 60-fold with the GRF4-GIF1-containing vectors as compared to the control vector and ranged from ~5% in the cultivar Kachu to 13% in the cultivar RL6077. We then edited two genes that confer resistance against leaf rust and powdery mildew directly in the aforementioned elite cultivars. A wheat promoter, TaU3 or TaU6, to drive the expression of guide RNA was effective in gene editing whereas the OsU3 promoter failed to generate any edits. Editing efficiency was nearly perfect with the wheat promoters. Our protocol has made it possible to edit genes directly in elite wheat cultivars and would be useful for gene editing in other wheat varieties, which have been recalcitrant to transformation thus far.
Journal Article
Lanthanum Prolongs Vase Life of Cut Tulip Flowers by Increasing Water Consumption and Concentrations of Sugars, Proteins and Chlorophylls
We evaluated the effect of separately adding two sources of lanthanum (La), LaCl
3
and La(NO
3
)
3
× 6H
2
O at a concentration of 40 µM each, to the preservative solution of 15 cut tulip flower varieties. Ascorbic acid (AsA; 0.2 g/L) was used as a reference solution, while distilled water was used as control. The variety Laura Fygi recorded the longest vase life with 13 days. The highest water consumption per gram of stem fresh biomass weight (FBW) (2.5 mL) was observed in the variety Violet Beauty, whereas the lowest (1.098 mL) was recorded in Pink Impression. At the end of the vase life period, higher concentrations of total soluble sugars in petals and total soluble proteins in leaves were recorded in La-treated stems, compared to the AsA treatment and the control. Additionally, La(NO
3
)
3
× 6H
2
O supply increased the fresh weight of stems in vase and prolonged vase life. Moreover, this treatment resulted in the highest foliar concentration of chlorophylls at the end of vase life. Therefore, La increases tulip flower vase life as a consequence of improving the concentrations of some vital biomolecules.
Journal Article
Modeling and Analyzing the Availability of Technical Professional Profiles for the Success of Smart Cities Projects in Europe
The success of developing and implementing Smart Cities (SC) projects depends on a varied set of factors, where the availability of a qualified technical workforce is a critical one. The combination of ICT requirements, like the effectiveness and quality of solutions merging IoT, cloud computing, sensors, and communications with the work from many varied disciplines (e.g., civil engineering, architecture, etc.), mixed with aspects of environmental and business sustainability, makes the management of these projects really challenging. Reports forecast a scarcity of qualified candidates, given this complexity and the growth of activity in SC projects. The European project SMACITE has addressed the requirements of the qualification of an ICT workforce with an analysis of multiples sources of information from the labor market, feedback from involved stakeholders, and the literature. The goal was the development of two occupational ICT profiles as a reference for training and for the availability of candidates for job vacancies. The result is two ICT role profiles for engineers and technicians, mapped with the European skills frameworks ESCO and EN16234. The profiles determined the whole set of requirements, including not only the technical areas and soft skills, but also additional technical areas and sustainability and managerial skills and the analysis of different sources of information. Our work has also determined which existing ESCO occupations are similar to the two reference profiles, so they are better adapted to SC projects. The training activities of SMACITE have also suggested the amount of training expected for a varied sample of candidates who want to be qualified for SC projects.
Journal Article
The burden of non-communicable disease risk factors in a low-income population: Findings from a cross-sectional study highlighting the prevalence of obesity, hypertension, and metabolic disorders in the south of Quito, Ecuador
We describe the prevalence of Non-Communicable Disease (NCD) risk factors in a low-income health district in the South of Quito, Ecuador.
We conducted a cross-sectional study between November 2020 and October 2021.
We used multi-stage cluster sampling to select 656 of población total adult residents of 17D06 health district, enabling a prevalence estimation with at least ±5.73% absolute precision. We collected socio-demographic information and behavioural risk factors using the expanded WHO STEPwise approach to NCD surveillance. We measured height, weight, and blood pressure, and collected blood samples to assess glucose levels, lipid profiles, and renal function. We estimated the prevalence of behavioural and metabolic NCD risk factors by sex and age groups (18-44, 45-69, and >70).
One-third of participants were obese (33.2% overall, 148 women, 38,7%, and 41 men, 22%), and more than half had a raised waist circumference (56.8%, N = 322). Hypertension affected 26.9% of participants (63 men, 33% and 90 women, 24%). Hyperglycaemia affected 7.9% (N = 45) of participants and increased with age and peaked at 22% among women over 70. More than half of the participants presented hypercholesterolemia (317 individuals, 56.2%). Low consumption of fruit and vegetables, high salt consumption and high sugar consumption were common in all population groups (88.4%, N = 580, 33.2%. N = 218 and 72.4%, N = 475, respectively).
The critical prevalence of NCD risk factors in this low-income urban district of Quito, alongside similar trends observed in other settings across Latin America, underscores the need for ecological public health strategies to create healthy environments and promote healthier behaviours. Gender-sensitive approaches may be useful to address differences between sexes.
Journal Article