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Vitiligo is an autoimmune skin disorder caused by dysfunctional pigment-producing melanocytes which are attacked by immune cells. Oxidative stress is considered to play a crucial role in activating consequent autoimmune responses related to vitiligo. Melanin synthesis by melanocytes is the main intracellular stressor, producing reactive oxygen species (ROS). Under normal physiological conditions, the antioxidative nuclear factor erythroid 2-related factor 2 (Nrf2) pathway functions as a crucial mediator for cells to resist oxidative stress. In pathological situations, such as with antioxidant defects or under inflammation, ROS accumulate and cause cell damage. Herein, we summarize events at the cellular level under excessive ROS in vitiligo and highlight exposure to melanocyte-specific antigens that trigger immune responses. Such responses lead to functional impairment and the death of melanocytes, which sequentially increase melanocyte cytotoxicity through both innate and adaptive immunity. This report provides new perspectives and advances our understanding of interrelationships between oxidative stress and autoimmunity in the pathogenesis of vitiligo. We describe progress with targeted antioxidant therapy, with the aim of providing potential therapeutic approaches.

Additive manufacturing (AM), also known as 3D printing, is a recent innovation in manufacturing, employing additive techniques rather than traditional subtractive methods. This study focuses on Directed Energy Deposition (DED), utilizing a blend of nickel-based superalloy IN 718 and stainless steel SS316 powders in varying ratios (25%+75%, 50%, and 75%+25%). The objective is to assess the impact of process parameters on quality and optimize them. Mechanical properties of the different powder mixtures are compared. In the study, Taguchi-grey relational analysis is employed for parameter optimization, with four key factors identified: laser power, overlap ratio, powder feed rate, and scanning speed, affecting cladding efficiency, deposition rate, and porosity. Verification experiments confirm optimization repeatability, and further fine-tuning is achieved through one-factor-at-a-time experiments. Optimized parameters yield varied tensile properties among different powder mixtures; for example, a 25% SS316L and 75% IN718 blend demonstrates the highest ultimate tensile strength (499.37 MPa), while a 50% SS316L and 50% IN718 blend exhibits the best elongation (13.53%). This study offers an effective approach for using DED technology to create mixed SS316 and IN718 powders, enabling tailored mechanical performance based on mixing ratios.

The rachises of extant feathers, composed of dense cortex and spongy internal medulla, are flexible and light, yet stiff enough to withstand the load required for flight, among other functions. Incomplete knowledge of early feathers prevents a full understanding of how cylindrical rachises have evolved. Bizarre feathers with unusually wide and flattened rachises, known as “rachisdominated feathers” (RDFs), have been observed in fossil nonavian and avian theropods. Newly discovered RDFs embedded in early Late Cretaceous Burmese ambers (about 99 million year ago) suggest the unusually wide and flattened rachises mainly consist of a dorsal cortex, lacking a medulla and a ventral cortex. Coupled with findings on extant feather morphogenesis, known fossil RDFs were categorized into three morphotypes based on their rachidial configurations. For each morphotype, potential developmental scenarios were depicted by referring to the rachidial development in chickens, and relative stiffness of each morphotype was estimated through functional simulations. The results suggest rachises of RDFs are developmentally equivalent to a variety of immature stages of cylindrical rachises. Similar rachidial morphotypes documented in extant penguins suggest that the RDFs are not unique to Mesozoic theropods, although they are likely to have evolved independently in extant penguins.

The niche microenvironment plays a crucial role in regulating the fate of normal skin stem cells (SSCs) and cancer stem cells (CSCs). Therapeutically targeting the CSC niche holds promise as an effective strategy; however, the dual effects of shared SSC niche signaling in CSCs have contributed to the aggressive characteristics of tumors and poor survival rates in skin cancer patients. The lack of a clear underlying mechanism has significantly hindered drug development for effective treatment. This article explores recent advances in understanding how niche factors regulate cell fate determination between skin stem cells and skin CSCs, along with their clinical implications. The dual roles of key components of the adhesive niche, including the dermo-epidermal junction and adherens junction, various cell types—especially immune cells and fibroblasts—as well as major signaling pathways such as Sonic hedgehog (Shh), Wingless-related integration site (Wnt)/β-catenin, YAP (Yes-associated protein)/TAZ (transcriptional coactivator with PDZ-binding motif), and Notch, are highlighted. Additionally, recent advances in clinical trials and drug development targeting these pathways are discussed. Overall, this review provides valuable insights into the complex interactions between skin cancer stem cells and their microenvironment, laying the groundwork for future research and clinical strategies.

Vitiligo is a chronic autoimmune depigmenting skin disorder characterized by patches of the skin losing functional melanocytes. Multiple combinatorial factors are involved in disease development, among which immune T cells play a prominent role. The immune cells implicated in melanocyte destruction through adaptive immunity include CD8 + cytotoxic T cells and regulatory T cells, and aberrantly activated skin-resident memory T cells also play a role in melanocyte destruction. Over the past several years, major progress in understanding vitiligo pathogenesis has led to the development of targeted therapies. Janus kinase (JAK) inhibitors, which share the similar mechanism that autoactivates CD8 + T cells in chronic inflammatory diseases, have been reported to have therapeutic significance in vitiligo. Recently, immunomodulatory therapeutic interventions in vitiligo have been emerging. Mesenchymal stem cells (MSCs) regulate cytokine secretion and the balance of T-cell subsets, which makes them a promising cell-based treatment option for autoimmune diseases. The induction of MSC-mediated immunomodulation is complicated and occurs by contact-dependent mechanisms and soluble extracellular vesicle (EV) mediators. EVs released from MSCs contain various growth factors and cytokines with anti-inflammatory effects in the skin immune response. Here, we summarize and discuss the progress to date in targeted therapies that immunomodulate the niche environment of vitiligo, from the clinical trial of JAK inhibitors to the potential of MSCs and MSC-EVs. The available information was collected to highlight the need for further research into the treatment of vitiligo.

With the development of Taiwanese society, the tourist B&B industry has become particularly important, marking the origin of the significant development of the tourism industry. This study focuses on the quality of host–tourist relationships in B&Bs. It is proposed that tourists “discover” the emotions and feelings of B&B hosts through service contact processes. Although researchers have pointed out that frontline service employee personality traits affect the quality of interactions and satisfaction from the consumers’ point of view, very few studies have investigated the relationships between tourists and B&B hosts, the latter playing a double role—both as a host and a service worker. Data were collected from 422 tourists who had utilized B&B services. A quantitative analysis of the questionnaires was conducted through descriptive statistics, K-means clustering, one-way ANOVA and structural equation modeling (SEM), in order to determine the relationships among the three sets of variables. The results of this study reveal that the personality traits of B&B hosts directly affect their emotional labor and the quality of their relationships with tourists. However, the emotional labor of B&B hosts is found not to affect the quality of relationships; in this respect, our findings go counter to those of previous studies.

Unsupervised domain adaptation (UDA) focuses on transferring knowledge from the labeled source domain to the unlabeled target domain, reducing the costs of manual data labeling. The main challenge in UDA is bridging the substantial feature distribution gap between the source and target domains. To address this, we propose Polarized Attention Network Domain Adaptation (PANDA), a novel approach that leverages Polarized Self-Attention (PSA) to capture the intricate relationships between the source and target domains, effectively mitigating domain discrepancies. PANDA integrates both channel and spatial information, allowing it to capture detailed features and overall structures simultaneously. Our proposed method significantly outperforms current state-of-the-art unsupervised domain adaptation (UDA) techniques for semantic segmentation tasks. Specifically, it achieves a notable improvement in mean intersection over union (mIoU), with a 0.2% increase for the GTA→Cityscapes benchmark and a substantial 1.4% gain for the SYNTHIA→Cityscapes benchmark. As a result, our method attains mIoU scores of 76.1% and 68.7%, respectively, which reflect meaningful advancements in model accuracy and domain adaptation performance.

The purpose of this study is to optimize the critical parameters of the directed energy deposition (DED) process using the response surface methodology (RSM) and central composite design (CCD). The experiments investigate the influence of three key process factors, namely laser power, powder feed rate, and scanning speed, on deposition efficiency, deposition rate (DR), and porosity. Additionally, through analysis of variance (ANOVA), the significant factors and interaction effects are identified, and predictive models are developed for quality prediction. The research successfully optimizes the process parameters, which are validated through the fabrication of geometric components, specifically thin-walled nozzles. The study introduces innovative approaches such as “plunge-cutting toolpath” and “hybrid laser head lift height (Z-offset) method” to address the challenges associated with complex geometries. Additionally, the reliability and practicality of the optimized process parameters are confirmed.

Zerumbone, a natural monocyclic sesquiterpene, is the main component of the tropical plant Zingiber zerumbet Smith. Zerumbone induced antiproliferative and apoptotic effects against PC-3 and DU-145, two human hormone-refractory prostate cancer (HRPC) cell lines. Zerumbone inhibited microtubule assembly and induced an increase of MPM-2 expression (specific recognition of mitotic proteins). It also caused an increase of phosphorylation of Bcl-2 and Bcl-xL, two key events in tubulin-binding effect, indicating tubulin-binding capability and mitotic arrest to zerumbone action. Furthermore, zerumbone induced several cellular effects distinct from tubulin-binding properties. First, zerumbone significantly increased, while paclitaxel (as a tubulin-binding control) decreased, Mcl-1 protein expression. Second, paclitaxel but not zerumbone induced Cdk1 activity. Third, zerumbone other than paclitaxel induced Cdc25C downregulation. The data suggest that, in addition to targeting tubulin/microtubule, zerumbone may act on other targets for signaling transduction. Zerumbone induced mitochondrial damage and endoplasmic reticulum (ER) stress as evidenced by the loss of mitochondrial membrane potential and upregulation of GRP-78 and CHOP/GADD153 expression. Zerumbone induced an increase of intracellular Ca 2+ levels, a crosstalk marker between ER stress and mitochondrial insult, associated with the formation of active calpain I fragment. It induced apoptosis through a caspase-dependent way and caused autophagy as evidenced by dramatic LC3-II formation. In summary, the data suggest that zerumbone is a multiple targeting compound that inhibits tubulin assembly and induces a crosstalk between ER stress and mitochondrial insult, leading to apoptosis and autophagy in HRPCs.

To investigate the distribution of lower extremity running injuries and their associated factors. Descriptive and exploratory study. 1004 participants of the 2005 ING Taipei International Marathon. We used a self-developed questionnaire to collect data of previous running injuries and applied multivariate logistic regression modeling to examine relationships between these injuries and associated factors. Of the 893 valid questionnaires, 396 (44.4%) reported having previous lower extremity pain related to running. Knee joint pain was the most common problem (32.5%). Hip pain was associated with the racing group, training duration, and medial arch support. Use of knee orthotics (P = 0.002) and ankle braces (P = 0.007) was related to a higher rate of knee and ankle pain. Participants of the full marathon group who practiced on a synthetic track had a higher incidence of ankle pain. A training duration of >60 min was linked to an increased rate of foot pain (P = 0.003). Our data indicated that running injuries were associated with training duration and use of orthotics. Clinicians can use this information in treating or preventing running associated injuries and pain.