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Maintaining stable crop yields is crucial for sustainable agriculture. This study investigated the impact of various fertilization regimes, combined with regional climate variables, on the yield stability of cabbage (Brassica oleracea L. var. capitata) in southern Taiwan. Conducted from 2011 to 2023 on well-maintained soils that were established in 1988, the study examined two crop rotation systems (R1 and R2) and three fertilization treatments: chemical fertilizer, integrated fertilizer, and organic fertilizer. Despite the consistent annual application of fertilizers, the regression analysis revealed a significant decline in cabbage yields across all six treatment combinations, mainly due to reduced weights of single cabbages. To identify contributing factors, the yield data were analyzed alongside weather and soil data, collected over more than a decade. A Pearson correlation analysis showed that increased sunshine duration, solar radiation, and higher relative humidity were significantly negatively correlated with cabbage yields in both the R1 and R2 rotation systems. Additionally, the regression tree analysis indicated that solar radiation exceeding 16.917 MJ m−2 per day was associated with lower yields. A further analysis of the total nitrogen accumulation revealed increasing nitrogen concentrations in the outer leaves of cabbages during this period, potentially contributing to the reduced head yields. These findings highlight that fertilization had a minimal influence on yield, even in well-established soils. Mitigating the effects of weather variables is, therefore, critical to reducing their adverse impact on crop yields.

This study compared the extractability of Cd, Cu, Ni, Pb, and Zn by 8 extraction protocols for 22 representative rural soils in Taiwan and correlated the extractable amounts of the metals with their uptake by Chinese cabbage for developing an empirical model to predict metal phytoavailability based on soil properties. Chemical agents in these protocols included dilute acids, neutral salts, and chelating agents, in addition to water and the Rhizon soil solution sampler. The highest concentrations of extractable metals were observed in the HCl extraction and the lowest in the Rhizon sampling method. The linear correlation coefficients between extractable metals in soil pools and metals in shoots were higher than those in roots. Correlations between extractable metal concentrations and soil properties were variable; soil pH, clay content, total metal content, and extractable metal concentration were considered together to simulate their combined effects on crop uptake by an empirical model. This combination improved the correlations to different extents for different extraction methods, particularly for Pb, for which the extractable amounts with any extraction protocol did not correlate with crop uptake by simple correlation analysis.

A platform for rapid and automated imaging and laser manipulation of zebrafish larvae is presented. It should permit large-scale chemical and genetic screens in this vertebrate organism. We demonstrate a high-throughput platform for cellular-resolution in vivo chemical and genetic screens on zebrafish larvae. The system automatically loads zebrafish from reservoirs or multiwell plates, and positions and rotates them for high-speed confocal imaging and laser manipulation of both superficial and deep organs within 19 s without damage. We performed small-scale test screening of retinal axon guidance mutants and neuronal regeneration assays in combination with femtosecond laser microsurgery.

Uncertainty remains over composting time and maturity degree for compost production. The objectives of this study were to establish maturity indicators for composting based on spectral and chemical components and to provide a reference for future composting management. Several indicators of composting were assessed for three commercial composts at 0, 7, 15, 30, 45, and 60 days during the germination of Chinese cabbage, including (1) central temperature, (2) moisture content, (3) pH, (4) electrical conductivity, (5) C/N ratio, (6) E4/E6 ratio, (7) fluorescence humification index (HIX), and (8) germination index (GI). We evaluated the optimal composting time using these indicators, reflecting the changes in hog manure, chicken manure, and agricultural by-product composts throughout their composting process to provide a basis for maturity time. The results showed that the E4/E6 ratio, C/N ratio, humic acid (HA), fulvic acid (FA), and germination rate, which reached a stable status after 30 days of composting, could be the indicators of “early-stage” maturity. In contrast, central temperature, electrical conductivity, HIX, and GI reached stable values after 45 days of composting and thus could be more suitable indicators of full maturity. Based on our results, we recommend a minimum composting time of 30 days to achieve primary maturity, while fully matured compost may be obtained after 45 days.

Using beneficial microorganisms, such as purple non-sulfur bacteria (PNSB), has shown enormous potential for improving plant growth and agricultural production. However, the full extent of their benefits and interactions with agricultural practices is yet to be fully understood. The present study aimed to investigate the use of PNSB in crop rotation practice, focusing on its impact on rice growth and yield. The experiment was conducted over two rice cropping seasons, with djulis grown between the rice as a rotation crop. The study shows that PNSB treatment increased the concentration of 5-aminolevulinic acid (5-ALA) in plants, indicating enhanced photosynthesis. Moreover, when combined with crop rotation, PNSB remarkably improved soil fertility. These combined benefits resulted in substantial increases in tiller numbers (163%), leaf chlorophyll content (13%), and lodging resistance (66%), compared to the untreated plants. The combined treatment also resulted in higher productive tillers per hill (112%), average grain per hill (65%), and grain fertility (26%). This led to increased grain yield (65%), shoot dry weight (15%), and harvest index (37%). The findings clearly suggest that the incorporation of PNSB in crop rotation strategies can significantly augment the growth and yield of rice crops. These insights, pivotal for sustainable rice cultivation, hold the potential to simultaneously tackle the pressing issues of global food security and climate change.

Background: The study aimed to look into the effectiveness of a 6-month health coaching intervention for HbA1c and healthy diet in the treatment of patients with type 2 diabetes. Methods: The study was carried out via a two-armed, randomized controlled trial that included 114 diabetic patients at a medical center in Taiwan. During the 6-month period, the intervention group had health coaching and usual care for 6 months, and the control group had usual care only. The outcome variables were HbA1c level and healthy diet for follow-up measurement in the third and sixth month. Results: The study discovered a significant decrease in HbA1c and health diet improvement after the 6-month health coaching. Patients in the intervention group decreased their daily intake of whole grains, fruits, meats and protein, and fats and oils while increasing their vegetables intake. Conclusions: Health coaching may be conducive to the blood sugar control and healthy diet of patients with type 2 diabetes. Further study on health coaching with higher-quality evidence is needed.

Recreational sports are important leisure activities. However, most studies investigating barrier factors and motivation for participation in recreational sports have been limited to specific areas (e.g., a city or school) or demographic groups (e.g., adolescents). Therefore, this study set out to gain a more comprehensive understanding of the behavioral and socioeconomic factors influencing interest in recreational sports participation in Taiwan, as well as to evaluate the effect of any urban-rural divide. This study analyzed data collected by the \"Taiwan Social Change Survey\" (program five, wave 3) \"Leisure Life\" questionnaire. We used hierarchical linear modeling to assess respondent interest in recreational sports participation and evaluated the influence of behavioral factors, socioeconomic factors, and residence location (urban/rural). Of the 2,146 participants in this study, 50.3% were male, and the average age was 43.9 years. Location of residence (urban/rural) accounted for 35.3% of the variation in interest in recreational sports participation, while the remaining 64.7% came from the individual level. Participants who lived in rural settings were less interested in recreational sports than their urban counterparts. Gender, educational attainment, participation frequency, health-motivated interest, and appearance-motivated interest were also associated with interest in recreational sports participation. Different communication strategies may be needed to effectively reach different demographic groups. We suggest that future public health campaigns aiming to increase recreational sports participation include tailored interventions and messages to effectively encourage leisure physical activities among all, regardless of demographic boundaries.

Acanthamoeba spp. are free-living amoebae found in various water sources, including domestic tap water. These amoebae are known to cause Acanthamoeba keratitis, a severe corneal infection that can lead to vision loss. Although Acanthamoeba keratitis is commonly associated with water exposure, the environmental factors that enhance Acanthamoeba adhesion to corneal epithelial cells remain poorly understood. In this study, we examined the effects of domestic tap water on Acanthamoeba adhesion and found that exposure to tap water significantly increased the number of adherent trophozoites, potentially enhancing their cytopathic effects on corneal cells. We hypothesized that the calcium ions present in tap water may play a key role in regulating amoebal adhesion. To investigate this further, we analyzed the expression levels of several adhesion-related genes following exposure to different water conditions. Our findings suggest that tap water facilitates Acanthamoeba attachment and may contribute to disease progression. Understanding the role of calcium and other potential regulatory factors in adhesion may provide new insights into Acanthamoeba keratitis pathogenesis and aid in the development of preventive strategies. Les Acanthamoeba spp. sont des amibes libres présentes dans diverses sources d’eau, notamment l’eau du robinet. Ces amibes sont connues pour provoquer la kératite à Acanthamoeba , une infection cornéenne grave pouvant entraîner une perte de vision. Bien que la kératite à Acanthamoeba soit fréquemment associée à l’exposition à l’eau, les facteurs environnementaux qui favorisent l’adhérence d’ Acanthamoeba aux cellules épithéliales cornéennes restent mal compris. Dans cette étude, nous avons examiné les effets de l’eau du robinet sur l’adhérence d’ Acanthamoeba et constaté que l’exposition à cette eau augmentait significativement le nombre de trophozoïtes adhérents, renforçant potentiellement leurs effets cytopathiques sur les cellules cornéennes. Nous avons émis l’hypothèse que les ions calcium présents dans l’eau du robinet pourraient jouer un rôle clé dans la régulation de l’adhérence des amibes. Afin d’approfondir cette hypothèse, nous avons analysé les niveaux d’expression de plusieurs gènes liés à l’adhérence après exposition à différentes conditions d’eau. Nos résultats suggèrent que l’eau du robinet favorise l’adhérence d’ Acanthamoeba et pourrait contribuer à la progression de la maladie. Comprendre le rôle du calcium et d’autres facteurs de régulation potentiels dans l’adhérence pourrait apporter de nouvelles perspectives sur la pathogenèse de la kératite à Acanthamoeba et contribuer à l’élaboration de stratégies préventives.

Acanthamoeba are a free-living protozoan whose pathogenic strain can cause severe human diseases, such as granulomatous encephalitis and keratitis. As such, the pathogenic mechanism between humans and Acanthamoeba is still unknown. In our previous study, we identified the secreted Acanthamoeba M28 aminopeptidase (M28AP) and then suggested that M28AP can degrade human C3b and iC3b for inhibiting the destruction of Acanthamoeba spp. with the human immune response. We constructed the produced the recombinant M28AP from a CHO cell, which is a mammalian expression system, to characterize the biochemical properties of Acanthamoeba M28AP. The recombinant M28AP more rapidly hydrolyzed Leu-AMC than Arg-AMC and could be inhibited by EDTA treatment. We show that recombinant M28AP can be delivered into the individual cell line and cause cell line apoptosis in a co-culture model. In conclusion, we successfully investigated the potential molecular characteristics of M28AP.

Introduction: along with the rapidly aging population in many countries around the world, the global prevalence of diabetes and suffering from diabetes-related depression have risen in middle-aged and elderly adults. However, given that psychological stress is deeply influenced by culture, gender inequality in these statistics is often exhibited and increases with age. The aim of this study was to explore the gender difference in diabetes distress among middle-aged and elderly diabetic patients. Methods: 395 participants from four hospitals were recruited for a cross-sectional survey. The Taiwan Diabetes Distress Scale (TDDS) was used to measure diabetes distress. Linear regression was conducted to assess the gender difference in different types of diabetes distress. Results: there was significant gender difference in each diabetes distress domain. In particular, men who had received diabetes education in the past six months seemed to be more concerned about diabetes complications and felt pressured to communicate with doctors. In addition, women seemed to be more affected by diabetes distress because of their marital status, especially for married women. Conclusions: diabetes distress seems to have significant gender differences; however, more longitudinal research is needed on the causal relationship between gender and diabetes distress.