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To enhance wrist impairment rehabilitation efficiency, self-rehabilitation training using healthy-side forearm sEMG was introduced, improving patient engagement and proprioception. A sEMG-based movement recognition and muscle force estimation algorithm was proposed to transmit the estimated results to a wrist rehabilitation robot. Dominant eigenvalues of raw forearm EMG signals were selected to construct a movement recognition model that included a BPNN, a voting decision, and an intensified algorithm. An experimental platform for muscle force estimation was established to measure sEMG under various loads. The linear fitting was performed between mean absolute values (MAVs) and external loads to derive static muscle force estimation models. A dynamic muscle force estimation model was established through linear fitting average MAVs. Volunteers wore EMG sensors and performed six typical movements to complete the verification experiment. The average accuracy of only BPNN was 90.7%, and after the addition of the voting decision and intensified algorithm, it was improved to 98.7%. In the resistance training, the measured and estimated muscle forces exhibited similar trends, with RMSE of 4.2 N for flexion/extension and 5.8 N for ulnar/radial deviation. Under two different speeds and loads, the theoretical and estimated values of dynamic muscle forces showed similar trends with almost no phase difference, and the estimation accuracy was better during flexion movements compared to radial deviations. The proposed algorithms had strong versatility and practicality, aiming to realize the self-rehabilitation trainings of patients.

In order to elucidate whether deep freezing could maintain the quality of snowflake beef, three different deep freezing temperatures (−18 °C, −40 °C, and −60 °C) were used in order to evaluate the changes in tissue structures, quality characteristics and spoilage indexes, and their comparative effects on the quality of snowflake beef. Compared to samples frozen at −18 °C, those stored at −40 °C and −60 °C took a shorter time to exceed the maximum ice crystallization zone (significantly reduced by 2–6 h). In terms of short-term storage, samples frozen at −40 °C and −60 °C had better tissue structure and lower drip loss rate than those frozen at −18 °C; significant differences between groups in drip loss were observed between −18 °C and −60 °C. Moreover, a better bright red color and lower shear force were maintained at −40 °C and −60 °C, with significant differences in shear force between the −18 °C group and the other two groups on day 60. Although there were significant effects on the inhibition of lipid and protein oxidation at −40 °C and −60 °C; no significant variation was observed between these two groups throughout storage. A similar phenomenon was found in flavor, with 1-pentanol identified as an important potential indicator of flavor change in snowflake beef during storage. This study demonstrated that −40 °C and −60 °C had favorable impacts on the quality maintenance of snowflake beef compared to −18 °C. These findings provide a theoretical basis for effective stability of snowflake beef quality during frozen storage.

N⁶-methyladenosine (m⁶A) regulates stability and translation of messenger RNA (mRNA) in various biological processes. In this work, we show that knockout of the m⁶A writer Mettl3 or the nuclear reader Ythdc1 in mouse embryonic stem cells increases chromatin accessibility and activates transcription in an m⁶A-dependent manner. We found that METTL3 deposits m⁶A modifications on chromosome-associated regulatory RNAs (carRNAs), including promoter-associated RNAs, enhancer RNAs, and repeat RNAs. YTHDC1 facilitates the decay of a subset of these m⁶A-modified RNAs, especially elements of the long interspersed element-1 family, through the nuclear exosome targeting–mediated nuclear degradation. Reducing m⁶A methylation by METTL3 depletion or site-specific m⁶A demethylation of selected carRNAs elevates the levels of carRNAs and promotes open chromatin state and downstream transcription. Collectively, our results reveal that m⁶A on carRNAs can globally tune chromatin state and transcription.

Purpose. This study investigated the prognostic value of the albumin-to-fibrinogen ratio (AFR) in patients with sepsis as a consequence of infection at various sites. Methods. A total of 300 patients with sepsis caused by various infection sites, who met the diagnostic criteria for sepsis hospitalized in the intensive care unit, were enrolled in this study. The observational endpoint was 28-day mortality. Cox proportional hazard regression analysis was performed to determine the potential prognostic factors for 28-day mortality in these septic patients. Receiver operating characteristic (ROC) curve analysis was used to evaluate and compare the prognostic factors for 28-day mortality. Results. Of 300 participants, 147 died, corresponding to a 28-day mortality of 49% (147/300). Baseline Acute Physiology and Chronic Health Evaluation (APACHE II) score (hazard ratio (HR) 1.18 (95% confidence interval (CI) 1.07–1.30); P<0.001), baseline lactic acid level (HR 1.27 (95% CI 1.08–1.50); P=0.005), the presence of septic shock (HR 21.44 (95% CI 2.51–182.76); P=0.005), and baseline AFR (HR 0.70 (95% CI 0.62–0.80); P<0.001) were independent prognostic factors for 28-day mortality in patients with sepsis according to multivariate Cox analysis. Baseline AFR was an effective predictor of 28-day mortality, with an area under the ROC curve (AUC) of 0.700, and a specificity and sensitivity of 90.8% and 42.1%, respectively. A low baseline AFR level was associated with increased 28-day sepsis-related mortality. The quadruple index, which included the APACHE II score, lactic acid, septic shock, and AFR, showed a more accurate predictive value for septic patients than the APACHE II score, lactic acid, septic shock, and AFR alone, with an AUC of 0.922, and specificity and sensitivity of 86.9% and 83.6%, respectively. Moreover, the triple index, which included the APACHE II score, lactic acid, and septic shock, showed a significantly lower prognostic value for 28-day mortality compared with the ROC curve of the quadruple index and triple index, with an AUC of 0.877 and specificity and sensitivity of 77.8% and 82.3%, respectively. Conclusions. The results of this study demonstrate that AFR is an independent protective factor for predicting 28-day mortality in patients with sepsis due to various infection sites. AFR combined with the APACHE II score, lactic acid, and septic shock showed a higher prognostic value for sepsis prognosis.

Background: The integration of mining and urban spaces in coal-resource-based cities holds significant implications for urban transformation and sustainable development. However, existing research lacks an in-depth analysis of its characteristics and driving factors. Methods: This study takes the central urban area of Huaibei City as a case, utilizing historical documents, POI data, and spatial analysis methods to explore the evolution patterns and influencing factors of mining–urban spatial integration. Standard deviation ellipse analysis was employed to examine historical spatial changes, while a binary logistic regression model and principal component analysis were constructed based on 300 m × 300 m grid units to assess the roles of 11 factors, including location, transportation, commerce, and natural environment. Results: The results indicate that mining–urban spatial integration exhibits characteristics of lag, clustering, transportation dominance, and continuity. Commercial activity density, particularly leisure, dining, and shopping facilities, serves as a core driving factor. Road network density, along with the areas of educational and residential zones, positively promotes integration, whereas water surface areas (such as subsidence zones) significantly inhibit it. Among high-integration areas, Xiangshan District stands as the most economically prosperous city center; Lieshan–Yangzhuang mining area blends traditional and modern elements; and Zhuzhuang–Zhangzhuang mining area reflects the industrial landscape post-transformation. Conclusions: The study reveals diverse integration patterns under the synergistic effects of multiple factors, providing a scientific basis for optimizing spatial layouts and coordinating mining–urban development in coal-resource-based cities. Future research should continue to pay attention to the dynamic changes of spatial integration of mining cities, explore more effective integrated development models, and promote the rational and efficient use of urban space and the sustainable development of cities.

Long noncoding RNAs (lncRNAs) play important roles in various biological processes such as proliferation, cell death and differentiation. Here, we show that a liver-enriched lncRNA, named liver fibrosis-associated lncRNA1 (lnc-LFAR1), promotes liver fibrosis. We demonstrate that lnc-LFAR1 silencing impairs hepatic stellate cells (HSCs) activation, reduces TGFβ-induced hepatocytes apoptosis in vitro and attenuates both CCl 4 - and bile duct ligation-induced liver fibrosis in mice. Lnc-LFAR1 promotes the binding of Smad2/3 to TGFβR1 and its phosphorylation in the cytoplasm. Lnc-LFAR1 binds directly to Smad2/3 and promotes transcription of TGFβ, Smad2, Smad3, Notch2 and Notch3 which, in turn, results in TGFβ and Notch pathway activation. We show that the TGFβ1/Smad2/3/lnc-LFAR1 pathway provides a positive feedback loop to increase Smad2/3 response and a novel link connecting TGFβ with Notch pathway. Our work identifies a liver-enriched lncRNA that regulates liver fibrogenesis and suggests it as a potential target for fibrosis treatment. Activated hepatic stellate cells are the principal contributors to liver fibrosis by secreting a variety of pro-fibrogenic cytokines . Here Zhang et al. demonstrate that a liver-enriched lncRNA, lnc-LFAR1, promotes liver fibrosis and HSC activation by activating TGFβ and Notch signaling.

Van der Waals (vdW) gaps with ångström-scale heights can confine molecules or ions to an ultimately small scale, providing an alternative way to tune material properties and explore microscopic phenomena. Modulation of the height of vdW gaps between two-dimensional (2D) materials is challenging due to the vdW interaction. Here we report a general approach to control the vdW gap by preadsorption of water molecules on the material surface. By controlling the saturation vapour pressure of water vapour, we can precisely control the adsorption level of water molecules and vary the height of the vdW gaps of MoS 2 homojunctions from 5.5 Å to 53.6 Å. This technique can be further applied to other homo- and heterojunctions, constructing controlled vdW gaps in 2D artificial superlattices and in 2D/3D and 3D/3D heterojunctions. Engineering the vdW gap has great practical potential to modulate the device performance, as evidenced by the vdW-gap-dependent diode characteristics of the MoS 2 /gap/MoS 2 junction. Our work introduces a general strategy of molecular preadsorption that can extend to various precursors, creating more tunability and variability in vdW material systems. By preadsorption of water molecules on a material surface, a controllable ångström-scale van der Waals (vdW) gap is created, which can be applied to other vdW material systems with controllable gaps.

Background There is a complex relationship between social anxiety and sleep quality. However, network analysis studies of associations between social anxiety and sleep quality are lacking, particularly among patients with breast cancer. The current study aimed to extend this research to a sample of patients with breast cancer and to examine symptom-level associations between social anxiety and sleep quality using network analysis. Methods Network analysis was conducted to explore their associations and identify bridge items of social anxiety and sleep quality. Results The network structure revealed 9 important edges between social anxiety and sleep quality. “Subjective sleep quality” had the highest EI value in the network. “Working difficulty under watching” and “Sleep disorders” had the highest BEI values in their own communities. Conclusion There are complex pathological correlation pathways between social anxiety and sleep quality in breast cancer patients. “Subjective sleep quality”, “Working difficulty under watching” and “Sleep disorders” have the potential to be intervention targets for sleep disorder-social anxiety comorbidity. Medical staff can take corresponding interventions according to the the centrality indices and bridge centrality indicators identified in this study, which is likely to effectively reduce the comorbidity of sleep disorders and social anxiety.

Postmenopausal osteoporosis (PMOP) is a prevalent form of primary osteoporosis, affecting over 40% of postmenopausal women. Previous studies have suggested a potential association between single nucleotide polymorphisms (SNPs) in glucagon-like peptide-1 receptor (GLP-1R) and PMOP in postmenopausal Chinese women. However, available evidence remains inconclusive. Therefore, this study aimed to investigate the possible association between GLP-1R SNPs and PMOP in Han Chinese women. Thus, we conducted a case-control study with 152 postmenopausal Han Chinese women aged 45–80 years, including 76 women with osteoporosis and 76 without osteoporosis. Seven SNPs of the GLP-1R were obtained from the National Center of Biotechnology Information and Genome Variation Server. We employed three genetic models to assess the association between GLP-1R genetic variants and osteoporosis in postmenopausal women, while also investigating SNP-SNP and SNP-environment interactions with the risk of PMOP. In this study, we selected seven GLP-1R SNPs (rs1042044, rs2268641, rs10305492, rs6923761, rs1126476, rs2268657, and rs2295006). Of these, the minor allele A of rs1042044 was significantly associated with an increased risk of PMOP. Genetic model analysis revealed that individuals carrying the A allele of rs1042044 had a higher risk of developing osteoporosis in the dominant model ( P = 0.029, OR = 2.76, 95%CI: 1.09–6.99). Furthermore, a multiplicative interaction was found between rs1042044 and rs2268641 that was associated with osteoporosis in postmenopausal women ( P interaction = 0.034). Importantly, this association remained independent of age, menopausal duration, family history of osteoporosis, and body mass index. However, no significant relationship was observed between GLP-1R haplotypes and PMOP. In conclusion, this study suggests a close association between the A allele on the GLP-1R rs1042044 and an increased risk of PMOP. Furthermore, this risk was significantly augmented by an SNP-SNP interaction with rs2268641. These results provide new scientific insights into the development of personalized prevention strategies and treatment approaches for PMOP.

Manganese ferrite (MnFe.sub.2O.sub.4) supported on activated alumina beads (MFO-AABs) was synthesized by solvothermal method and used to activate periodate (PI) to degrade crystal violet (CV) and other organic dye pollutants. Under the optimum conditions (MFO-AABs dosage of 10 beads and PI dosage of 0.4 g/L), the removal efficiency of 10 mg/L CV reached 98.3% in 120 min. This surpassed the sum of removal efficiencies when MFO-AABs and PI presented respectively, indicating that MFO-AABs and PI worked synergistically. The removal efficiency of CV was almost unaffected in the presence of SO.sub.4.sup.2-(aq.) while slightly inhibited by Cl.sup.-(aq.), HCO.sub.3.sup.-(aq.) or HPO.sub.4.sup.2-(aq.). The effect of initial pH was studied in the range of 3-9, showing that the removal performance of CV was better under acidic and neutral conditions. Reusability tests showed that the removal efficiency of CV declined slightly after MFO-AABs was reused for 4 times, which was attributed to the accumulation of degradation products on the catalyst. Through calcination to remove these adsorbed degradation products, the catalytic ability could be recovered. Quenching experiments showed that .sup.1O.sub.2 played a major role in the reaction process. The contribution of non-radical electron transfer was ruled out by a series of electrochemical tests. In addition, IO.sub.4.sup.-(aq.) was stoichiometrically converted to IO.sub.3.sup.-(aq.) without producing potentially toxic iodine species such as I.sup.-(aq.), I.sub.2/I.sub.3.sup.-(aq.) and HOI. Combining liquid chromatography-mass spectrometry (LC-MS) and density functional theory (DFT) calculations, the possible degradation pathway of CV was proposed with 12 degradation products. Finally, the analysis of potential toxicity was carried out by theoretical calculations as well as experiments with Vigna radiata, revealing the decreased potential toxicity after the degradation process.