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Water inrush from confined aquifers in coal seam floor poses a great threat to the safety production of coal mines, while the aquiclude in coal seam floor is a key factor to avoid water inrush disasters. Therefore, an objective and accurate evaluation on the water-resisting ability of the aquiclude can effectively identify potential risk areas of water inrush and plays an important role in preventing such disasters. In this study, Longfeng coal mine was taken as an example. And based on the availability of data and the representativeness of factors, thickness of effective aquiclude, brittle rock thickness of aquiclude, rock quality designation of aquiclude, consumption of drilling fluid, mudstone ratio of aquiclude and fault fracture zone were selected as evaluation factors for water-resisting ability. Besides, the non-linear mathematical method—entropy weight method was combined to construct a comprehensive evaluation model of the water-resisting ability of aquiclude in coal seam floor. On this basis, the information processing and spatial display functions of GIS technology were used to make a thematic map of influencing factors and the thematic map was compositely superimposed. Finally, the evaluation on the water-resisting ability of aquiclude in coal seam floor in Longfeng coal mine was realized. The multi-source information fusion evaluation method based on GIS and entropy weight can comprehensively and objectively reflect the feature that the water-resisting ability of aquiclude in coal seam floor was controlled by multiple factors and had complex formation mechanisms. Also, the evaluation results were displayed intuitively and visually and can provide a scientific basis for the prevention and control of water inrush disasters from coal seam floor.

The use of cement-based composites (CBC) with high thermal conductivity for geothermal well cementing is extremely important for the efficient development and use of geothermal energy. Accurate prediction of thermal conductivity can save a lot of experimental costs and time. At present, there is no specific calculation model for the thermal conductivity of CBC. In this study, the microstructure, thermal conductivity model and influencing factors of CBC were investigated by experimental tests, theoretical analysis and numerical simulation. The results showed that the cement-based material could be simplified into a two-layer structure of hydrated and unhydrated layers. Mathematical and numerical models based on the coupled Series model and the Maxwell–Eucken model were established to calculate the thermal conductivity for CBC. The mathematical and numerical models were found to be more accurate by comparison with the conventional models and experimental test results. The cubic packing was more favorable than the spherical packing to improve the thermal conductivity of CBC. The plate material had significant anisotropy. The thermal conductivity of CBC showed a rapid decrease followed by a slow decrease, a decrease followed by a slow increase and finally a rapid decrease, a rapid increase followed by an up and down fluctuation and finally a plateau, respectively, with the increase of filler particle diameter, spacing and curing temperature. Based on these results, the effective methods and future research directions were proposed to maximize the thermal conductivity of geothermal well cementing materials in actual engineering applications. The research findings can provide some technical references for the efficient development of geothermal energy and research on CBC with high thermal conductivity.

当前，我国农村生活污水治理水平低与农业缺水并存，学界呼吁跳出单纯污水处理的传统思维，探索适度处理与资源利用相结合的新模式。近年来，我国农村生活污水治理工作得到重视、成效显著，但仍面临理念滞后、治理体系不完善、标准规范欠缺、系统谋划不足等问题。本文在梳理农村生活污水资源利用研究进展的基础上，分析其工作成效和现实困境，并分别从系统观念、考核办法、标准制（修）订、举措创新、试点示范等方面，提出资源利用实现路径，以期为我国农村生活污水治理提供技术支撑。The lack of domestic sewage governance in Chinese rural areas and the shortage of water for agriculture have led the academic community to call for breaking away from the traditional thinking of pure sewage treatment and developing a new model that combines the moderate treatment of rural domestic sewage with resource use. China's rural domestic sewage governance has recently received attention, and remarkable progress has been achieved, but prominent problems, such as the lag in governance, the imperfections in the governance system, the lack of standards and specifications, and the lack of systematic planning, need to be addressed. This paper summarizes the research progress on the resource use of rural domestic sewage, analyzes its progress and practical difficulties, and proposes a pat

ASH1L potentially contributes to Tourette syndrome (TS) and other neuropsychiatric disorders, as our previous studies have shown. It regulates essential developmental genes by counteracting polycomb-mediated transcriptional repression, which restricts chromatin accessibility at target genes. ASH1L is highly expressed in the adult brain, playing a crucial role in the early stage. However, it remains unclear how ASH1L mutations carried by patients with TS participate in regulating neuronal growth processes leading to TS traits. Five TS families recruited in our study underwent comprehensive physical examinations and questionnaires to record clinical phenotypes and environmental impact factors. We validated the variants via Sanger sequencing and constructed two mutants near the catalytic domain of ASH1L. We conducted molecular modeling, in vitro assays, and primary neuron cultures to find the role of ASH1L in neuronal development and its correlation with TS. In this study, we validated five pathogenic ASH1L rare variants and observed symptoms in patients with simple tics and behavioral comorbidities. Mutations near the catalytic domain of TS patients cause mental state abnormalities and disrupt ASH1L function by destabilizing its spatial conformation, leading to decreased activity of catalytic H3K4, thereby affecting the neurite growth. We need to conduct larger-scale studies on TS patients and perform additional neurological evaluations on mature neurons. We first reported the effects of ASH1L mutations in TS patients, including phenotypic heterogeneity, protein function, and neurological growth. This information contributes to understanding the neurodevelopmental pathogenesis of TS in patients with ASH1L mutations.

Zeolitic imidazolate framework‐67 (ZIF‐67) has emerged as a promising carrier for active substances (AS) due to its biocompatibility and acid‐triggered degradation behavior. Conventional loading strategies often involve multi‐step post‐synthetic modifications or one‐pot processes employing toxic organic solvents. This study presents a facile one‐pot synthesis method for encapsulating AS into ZIF‐67 in deionized water within 2 h under ambient conditions. This method exhibits broad applicability, enabling effective encapsulation of both hydrophobic and hydrophilic molecules with molecular weights ranging from low to high, with loading efficiencies between 12.17% and 56.11%. While free compounds were released rapidly in a pH‐independent manner, both CA@ZIF‐67 and EGCG@ZIF‐67 proved pH‐responsive sustained release profiles, with their mechanisms best described by the first‐order model and Fickian diffusion, respectively. Moreover, these composites demonstrated synergistic and pH‐dependent antibacterial activity against Escherichia coli and Staphylococcus aureus, showing significantly enhanced efficacy in acidic conditions. Furthermore, the formation process, morphological evolution, and release behavior of AS@ZIF‐67 composites were elucidated through Monte Carlo simulations, modified attachment energy theory, and molecular dynamics simulations. This green one‐pot strategy offers a versatile platform for applications in controlled release, antimicrobial systems, and targeted delivery. Zeolitic imidazolate framework‐67 (ZIF‐67) was successfully synthesized in deionized water for 2 h in this work; meanwhile, various active substances could be encapsulated in one‐pot with the same process, resulting in high loading rates. The study delved into the growth mechanism, morphological difference and loading capacity. Additionally, the in vitro synergistic antibacterial performance of AS@ZIF‐67 was verified due to its pH‐responsive release performance.

Overexpression of Medicago SOC1a leads to precocious primary shoot axis elongation. Abstract Medicago flowering, like that of Arabidopsis, is promoted by vernalization and long days, but alternative mechanisms are predicted because Medicago lacks the key regulators CO and FLC. Three Medicago SOC1-like genes, including MtSOC1a, were previously implicated in flowering control, but no legume soc1 mutants with altered flowering were reported. Here, reverse transciption-quantitative PCR (RT-qPCR) indicated that the timing and magnitude of MtSOC1a expression was regulated by the flowering promoter FTa1, while in situ hybridization indicated that MtSOC1a expression increased in the shoot apical meristem during the floral transition. A Mtsoc1a mutant showed delayed flowering and short primary stems. Overexpression of MtSOC1a partially rescued the flowering of Mtsoc1a, but caused a dramatic increase in primary stem height, well before the transition to flowering. Internode cell length correlated with stem height, indicating that MtSOC1a promotes cell elongation in the primary stem. However, application of gibberellin (GA3) caused stem elongation in both the wild type and Mtsoc1a, indicating that the mutant was not defective in gibberellin responsiveness. These results indicate that MtSOC1a may function as a floral integrator gene and promotes primary stem elongation. Overall, this study suggests that apart from some conservation with the Arabidopsis flowering network, MtSOC1a has a novel role in regulating aspects of shoot architecture.

Poa alpigena Lindm. is a dominant forage grass that is widely distributed on the Qinghai-Tibetan Plateau and is often used in the restoration of degraded grasslands. Soil microorganisms are major players in the cycling of materials in terrestrial ecosystems. In this study, based on high-throughput sequencing, the rhizosphere and non-rhizosphere soils of Poa alpigena L. on Bird Island, Qinghai Lake, were used to investigate the effects of Poa alpigena L. on the composition and structure of soil microbial communities, and to establish associated soil microbial gene pools. Results revealed that microorganisms in the soil of Poa alpigena L. on Bird Island belonged to 62 phyla, 112 classes, 245 orders, 518 families, 1610 genera, and 5704 species. The dominant soil bacteria in rhizosphere and non-rhizosphere soils were Proteobacteria (49.62%, 47.13%) and Actinobacteria (30.31% and 31.67%), whereas the dominant fungi were Ascomycota (3.15% and 3.37%) and Basidiomycota (0.98% and 1.06%). Alpha diversity analysis revealed that the microbial richness and diversity in non-rhizosphere soil were significantly higher than those in rhizosphere soil, mainly influenced by soil water content and total nitrogen content. Furthermore, on the basis of LEfSe analysis, Alphaproteobacteria and Betaproteobacteria were identified as prominent differential taxa for rhizosphere and non-rhizosphere soils, respectively. The key differential metabolic pathways of rhizosphere soil microorganisms were those associated with the ATP-binding cassette (ABC) transporter, basal metabolism, and cytochrome P450 metabolism, whereas those of non-rhizosphere soil microorganisms included the gene expression-related pathways, methane metabolism, and pathway associated with degradation of aromatic compounds. These findings indicated that the rhizosphere soil of Poa alpigena L. is selective for microorganisms that play important roles in the oxidation of methane and regulation of the greenhouse effect on Bird Island, and that the soil environment on this island may be subject to contamination with aromatic compounds.

Against the backdrop of the global trend toward delayed childbearing, elucidating the mechanisms underlying uterine aging has emerged as a critical biomedical priority for addressing age‐related implantation failure. Through unbiased global metabolomic profiling of peri‐implantation uteri across different ages in mice, we identified nicotinamide adenine dinucleotide (NAD+) depletion as a hallmark metabolic feature of endometrial aging. Single‐cell RNA sequencing further revealed an expansion of senescent stromal cell populations, which was accompanied by a decline in NAD+ levels. Supplementation with NAD+ precursors alleviated age‐related stromal senescence and endometrial dysfunction, thereby restoring the uterus' implantation competence. Mechanically, we demonstrate that CD38 derived from myeloid serves as a principal driver of uterine NAD+ depletion; this process accelerates stromal senescence and impairs uterine receptivity. These findings establish CD38 as a central physiological integrator that links NAD+ metabolism to uterine function and highlight it as a promising target for rejuvenation strategies aimed at improving reproductive outcomes in women of advanced maternal age. An increased population of CD38+ macrophages, accompanied by a marked decline in stromal cell NAD+ levels, serves as key hallmarks of endometrial aging in aged mice. Pharmacological and genetic interventions revealed that macrophage‐derived CD38 is a principal driver of uterine NAD+ depletion, thereby accelerating stromal cell senescence and impairing uterine receptivity.

Objective Tourette syndrome (TS) is a childhood neurodevelopmental disorder caused by various genetic and environmental factors and presents with apparent genetic heterogeneity. As ASH1L potentially contributes to neurodevelopmental diseases, especially in TS, we aim to investigate the susceptibility of ASH1L on TS in the Chinese Han population. Methods Three tag single nucleotide polymorphisms (SNPs) (rs5005770, rs12734374, and rs35615695) in ASH1L were screened in 271 TS nuclear family trios and 337 healthy subjects by the TaqMan assays real time. A case–control study combined with family‐based analysis was applied to study the genetic susceptibility of common variants of ASH1L. Results The results revealed a significant over‐transmission of rs35615695 and rs5005770 (for rs35615695, transmission disequilibrium test, χ2 = 57.375, p = .000, HHRR, χ2 = 4.807, p = .028; for rs5005770, HRR, χ2 = 4.116, p = .042, HHRR, χ2 = 8.223, p = .004) in family‐based study. Furthermore, rs5005770 and rs35615695 still remained significant after Bonferroni correction (p < .017). However, the two SNPs (rs5005770 and rs35615695) were found not to be associated with TS in case–control study. Conclusions Our study suggests that ASH1L may contribute to TS susceptibility in the Han Chinese population and involved in TS development as a risk factor. Tourette syndrome is a childhood neurodevelopmental disorder, caused by a variety of genetic and environmental factors and presents with obvious genetic heterogeneity. As ASH1L potentially contributes to neurodevelopmental diseases, especially in TS, we aim to investigate the susceptibility of ASH1L on TS in the Chinese Han population. Three tag single nucleotide polymorphisms (SNPs) (rs5005770, rs12734374, and rs35615695) in ASH1L were screened in 271 TS nuclear family trios and 337 healthy subjects by the TaqMan assays real time. A combination of case–control study with family‐based analysis was applied to study the genetic susceptibility of common variants of ASH1L. Our study suggests that Ash1L may contribute to TS susceptibility in the Han Chinese population and as a risk factor involved in the development of TS.

Tourette syndrome (TS) is caused by complex genetic and environmental factors and is characterized by tics. Histidine decarboxylase (HDC) mutation is a rare genetic cause with high penetrance in patients with TS. HDC‐knockout (KO) mice have similar behavioral and neurochemical abnormalities as patients with TS. Therefore, HDC‐KO mice are considered a valuable TS pathophysiological model as it reveals the underlying pathological mechanisms that cannot be obtained from patients with TS, thus advancing the development of treatment strategies for TS and other tic disorders. This review summarizes some of the recent research hotspots and progress in HDC‐KO mice, aiming to deepen our understanding of brain mechanisms relevant to TS. Furthermore, we encapsulate the possible brain nerve cell changes in HDC‐KO mice and their potential roles in TS to provide multiple directions for the future research on tics. Tourette syndrome (TS) is caused by complex genetic and environmental factors and is characterized by tics. Histidine decarboxylase (HDC) mutation is a rare genetic cause with high penetrance in patients with TS. HDC‐knockout (KO) mice have similar behavioral and neurochemical abnormalities as patients with TS. Therefore, HDC‐KO mice are considered a valuable TS pathophysiological model as it reveals the underlying pathological mechanisms that cannot be obtained from patients with TS, thus advancing the development of treatment strategies for TS and other tic disorders. This review summarizes some of the recent research hotspots and progress in HDC‐KO mice, aiming to deepen our understanding of brain mechanisms relevant to TS. Furthermore, we also encapsulate the possible brain nerve cell changes in HDC‐KO mice and their potential roles in TS, to provide multiple directions for the future research on tics.