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Exchange magnons are essential for unprecedented miniaturization of GHz electronics and magnon-based logic. However, their efficient excitation via microwave fields is still a challenge. Current methods including nanocontacts and grating couplers require advanced nanofabrication tools which limit the broad usage. Here, we report efficient emission and detection of exchange magnons using micron-sized coplanar waveguides (CPWs) into which we integrated ferromagnetic (m) layers. We excited magnons in a broad frequency band with wavelengths λ down to 100 nm propagating over macroscopic distances in thin yttrium iron garnet. Applying time- and spatially resolved Brillouin light scattering as well as micromagnetic simulations we evidence a significant wavelength conversion process near mCPWs via tunable inhomogeneous fields. We show how optimized mCPWs can form microwave-to-magnon transducers providing phase-coherent exchange magnons with λ of 37 nm. Without any nanofabrication they allow one to harvest the advantages of nanomagnonics by antenna designs exploited in conventional microwave circuits. Magnons - collective excitations of electron spins - promise compact and fast electronics. However, the generation of short wave magnons is still quite challenging. Here, the authors demonstrate that by introducing a ferromagnetic layer, conventional coplanar waveguides can be used to efficiently generate such magnons.

Systemic acquired resistance (SAR) is induced by pathogens and confers protection against a broad range of pathogens. Several SAR signals have been characterized, but the nature of the other unknown signalling by small metabolites in SAR remains unclear. Glutathione (GSH) has long been implicated in the defence reaction against biotic stress. However, the mechanism that GSH increases plant tolerance against virus infection is not entirely known. Here, a combination of a chemical, virus‐induced gene‐silencing‐based genetics approach, and transgenic technology was undertaken to investigate the role of GSH in plant viral resistance in Nicotiana benthamiana. Tobacco mosaic virus (TMV) infection results in increasing the expression of GSH biosynthesis genes NbECS and NbGS, and GSH content. Silencing of NbECS or NbGS accelerated oxidative damage, increased accumulation of reactive oxygen species (ROS), compromised plant resistance to TMV, and suppressed the salicylic acid (SA)‐mediated signalling pathway. Application of GSH or l‐2‐oxothiazolidine‐4‐carboxylic acid (a GSH activator) alleviated oxidative damage, decreased accumulation of ROS, elevated plant local and systemic resistance, enhanced the SA‐mediated signalling pathway, and increased the expression of ROS scavenging‐related genes. However, treatment with buthionine sulfoximine (a GSH inhibitor) accelerated oxidative damage, elevated ROS accumulation, compromised plant systemic resistance, suppressed the SA‐mediated signalling pathway, and reduced the expression of ROS‐regulating genes. Overexpression of NbECS reduced oxidative damage, decreased accumulation of ROS, increased resistance to TMV, activated the SA‐mediated signalling pathway, and increased the expression of the ROS scavenging‐related genes. We present molecular evidence suggesting GSH is essential for both local and systemic resistance of N. benthamiana to TMV through a differential modulation of SA and ROS. Glutathione is required for both local and systemic resistance of Nicotiana benthamiana to tobacco mosaic virus infection through a differential modulation of salicylic acid signalling and reactive oxygen species.

In the age of labor shortage, increasing the throughput of warehouses is a good issue. In the recent two decades, automatic warehouses designed to reduce human labor have therefore become a very hot research topic. Tricycle forklifts being able to carry heavy goods can play important roles in automatic warehouses. Meanwhile, Robot Operating System (ROS) is a very famous and popular platform for developing the software of robotics. Its powerful communication function makes lots of warehouse information exchange easy. Therefore, ROS installed as the communication backbone of warehouse is very popular. However, the software modules of ROS do not offer tricycle forklifts. Therefore, in this research, the model of a tricycle forklift developed for ROS systems in warehouse applications is constructed. In spite of the developed model, the existing software modules must be modified for compatible connection such that the tricycle forklift can be navigated and controlled by constructed ROS. For the function of Simultaneous Localization And Mapping (SLAM) and the control of self-guided navigation, the constructed system is verified by Gazebo simulation. In addition, the experiments of a real tricycle forklift to demonstrate the developed ROS for enough accuracy of warehouse application are also included.

Skyrmions and antiskyrmions are topologically protected spin structures with opposite vorticities. Particularly in coexisting phases, these two types of magnetic quasi-particles may show fascinating physics and potential for spintronic devices. While skyrmions are observed in a wide range of materials, until now antiskyrmions were exclusive to materials with D 2d symmetry. In this work, we show first and second-order antiskyrmions stabilized by magnetic dipole–dipole interaction in Fe/Gd-based multilayers. We modify the magnetic properties of the multilayers by Ir insertion layers. Using Lorentz transmission electron microscopy imaging, we observe coexisting antiskyrmions, Bloch skyrmions, and type-2 bubbles and determine the range of material properties and magnetic fields where the different spin objects form and dissipate. We perform micromagnetic simulations to obtain more insight into the studied system and conclude that the reduction of saturation magnetization and uniaxial magnetic anisotropy leads to the existence of this zoo of different spin objects and that they are primarily stabilized by dipolar interaction. Antiskyrmions are topological spin textures with negative vorticity. Like skyrmions, they have considerable technological promise, but have only been stabilised in Heusler compounds. Here, Heigl et al. succeed in stabilising first and second order antiskyrmions in a new class of materials.

Alpha‐momorcharin (α‐MMC), a member of the plant ribosomal inactivating proteins (RIPs) family, has been proven to exhibit important biological properties in animals, including antiviral, antimicrobial, and antitumour activities. However, the mechanism by which α‐MMC increases plant resistance to viral infections remains unclear. To study the effect of α‐MMC on plant viral defence and how α‐MMC increases plant resistance to viruses, recombinant DNA and transgenic technologies were employed to investigate the role of α‐MMC in Nicotiana benthamiana resistance to tobacco mosaic virus (TMV) infection. Treatment with α‐MMC produced through DNA recombinant technology or overexpression of α‐MMC mediated by transgenic technology alleviated TMV‐induced oxidative damage and reduced the accumulation of reactive oxygen species (ROS) during TMV‐green fluorescent protein infection of N. benthamiana. There was a significant decrease in TMV replication in the upper leaves following local α‐MMC treatment and in α‐MMC‐overexpressing plants relative to control plants. These results suggest that application or overexpression of α‐MMC in N. benthamiana increases resistance to TMV infection. Finally, our results showed that overexpression of α‐MMC up‐regulated the expression of ROS scavenging‐related genes. α‐MMC confers resistance to TMV infection by means of modulating ROS homeostasis through controlling the expression of antioxidant enzyme‐encoding genes. Overall, our study revealed a new crosstalk mechanism between α‐MMC and ROS during resistance to viral infection and provides a framework to understand the molecular mechanisms of α‐MMC in plant defence against viral pathogens. Application or overexpression of α‐MMC in Nicotiana benthamiana increased resistance to TMV infection by means of modulating ROS homeostasis through controlling the expression of antioxidant enzyme‐encoding genes.

Research on grief among family caregivers of individuals with dementia has seen a notable increase. Our objective was to synthesize the relationship between coping factors and pre-death grief (PDG). (Prospero protocol: CRD42024560208) We conducted a systematic review of literature from PubMed, Web of Science, Scopus, PsycInfo, and Medline up to July 2024. Included studies encompassed quantitative, qualitative, and mixed methods approaches. During the study selection process, we excluded data on intervention effectiveness and studies not published in English. The quality of the studies was evaluated using the Mixed Methods Appraisal Tool. Evidence was summarized narratively. Participants in this study are family caregivers who take care of dementia patients. We included data from 12 studies in our analysis. The majority of these investigations were carried out in Western countries. The research primarily involved spousal or adult child caregivers and centered on PDG. We included validated measures of PDG in each study. Among the reviewed studies, five reported on coping strategies, while seven addressed coping resources. Overall, the findings indicated that the application of coping strategies, specifically positive coping strategies, is effective in alleviating PDG and mitigating the effects of caregiving burden on PDG. Coping resources - including self-efficacy, sense of coherence, and support from friends and family - appear to have a beneficial impact in reducing PDG. Additionally, the quality of relationships with friends and family members was found to be a significant factor. Moreover, spiritual and religious beliefs, along with community faith, have been identified as crucial elements in alleviating grief experienced by caregivers. Knowing what coping strategies and resources are beneficial to decrease PDG experiences among dementia caregivers.

In chloroplasts, insertion of proteins with multiple transmembrane domains (TMDs) into thylakoid membranes usually occurs in a co-translational manner. Here, we have characterized a thylakoid protein designated FPB1 (Facilitator of PsbB biogenesis1) which together with a previously reported factor PAM68 (Photosynthesis Affected Mutant68) is involved in assisting the biogenesis of CP47, a subunit of the Photosystem II (PSII) core. Analysis by ribosome profiling reveals increased ribosome stalling when the last TMD segment of CP47 emerges from the ribosomal tunnel in fpb1 and pam68 . FPB1 interacts with PAM68 and both proteins coimmunoprecipitate with SecY/E and Alb3 as well as with some ribosomal components. Thus, our data indicate that, in coordination with the SecY/E translocon and the Alb3 integrase, FPB1 synergistically cooperates with PAM68 to facilitate the co-translational integration of the last two CP47 TMDs and the large loop between them into thylakoids and the PSII core complex. The assembly of the Photosystem II proximal antenna CP47 remains a challenging question. Here the authors show that FPB1 and PAM68 act coordinately with Alb3 and the SecY/E translocon to facilitate the co-translational integration of specific regions of CP47 into thylakoids.

For many important crops including sorghum, use of CRISPR/Cas technology is limited not only by the delivery of the gene-modification components into a plant cell, but also by the ability to regenerate a fertile plant from the engineered cell through tissue culture. Here, we report that Wuschel2 (Wus2) -enabled transformation increases not only the transformation efficiency, but also the CRISPR/Cas-targeted genome editing frequency in sorghum ( Sorghum bicolor L .). Using Agrobacterium -mediated transformation, we have demonstrated Wus2 -induced direct somatic embryo formation and regeneration, bypassing genotype-dependent callus formation and significantly shortening the tissue culture cycle time. This method also increased the regeneration capacity that resulted in higher transformation efficiency across different sorghum varieties. Subsequently, advanced excision systems and “altruistic” transformation technology have been developed to generate high-quality morphogenic gene-free and/or selectable marker-free sorghum events. Finally, we demonstrate up to 6.8-fold increase in CRISPR/Cas9-mediated gene dropout frequency using Wus2 -enabled transformation, compared to without Wus2 , across various targeted loci in different sorghum genotypes. Che et al. use Wuschel2-enabled genome transformation to induce somatic embryo formation in sorghum, a grain used in human food. Their approach not only overcomes the genotype-dependent barrier for genetic transformation without the introduction of morphogenic genes, but also increases the frequency of CRISPR/Castargeted genome editing.

Stresses leading to the accumulation of misfolded proteins in the endoplasmic reticulum (ER) elicit a highly conserved ER stress response in plants called the unfolded protein response (UPR). While the response itself is well documented in plants, the components of the signaling pathway are less well known. We have identified three membrane-associated basic domain/leucine zipper (bZIP) factors in Arabidopsis thaliana that are candidates for ER stress sensors/transducers. One of these factors, bZIP28, an ER-resident transcription factor, is activated in response to treatment by tunicamycin (TM), an agent that blocks N-linked protein glycosylation. Following TM treatment, bZIP28 is processed, releasing its N-terminal, cytoplasm-facing domain, which is translocated to the nucleus. Expression of a truncated form of bZIP28, containing only the cytoplasmic domain of the protein, upregulated the expression of ER stress response genes in the absence of stress conditions. Thus, bZIP28 serves as a sensor/transducer in Arabidopsis to mediate ER stress responses related to UPR.

In light of the increased demand for long-term care services in China, there is an ongoing discussion on what factors contribute to older adults’ intended use of long-term care services. This study empirically recruited 239 community-dwelling older adults aged ≥60 years in China and explored factors influencing their intended use of four types of long-term care (basic life care, basic medical care, rehabilitation care, and psychological care) based on the Andersen behavioral model (i.e., predisposing characteristics, enabling resources, and need factors). The results showed that older adults were most likely to use psychological care. Age (as the predisposing characteristic) was the significant predictor of the intended use of four types of care. Regarding the intended use of basic life care, the enabling resources of marital status, household composition, income, as well as need factors of preference for the care setting, were influential. Moreover, income and need factors of self-rated physical health status were only two variables associated with the intended use of basic medical care. Concerning the intended use of rehabilitation care, household composition, income, self-rated physical health status, and preference for the care setting were significant predictors. The intended use of psychological care was influenced by enabling resources of marital status, household composition, and need factors of self-rated physical health status, preference for the care setting, and preference for the caregiver. These results can promote the sensitivity of policymakers and caregivers to the community-dwelling older adults’ intended use of long-term care and contribute to the delivery of appropriate care services by public policy.