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Background Cinnamomum camphora has been cultivated as an economically important tree for its medicinal and aromatic properties. Selective breeding has produced Cinnamomum plants for special uses, including spice strains with characteristic flavors and aromas and high-potency medicinal cultivars. The molecular biology underlying terpenoid biosynthesis is still unexplored. Results Gas chromatography-mass spectrometry was used to analyze the differences in contents and compositions of essential oil terpenoids in linalool- and borneol-type chemotypes of C. camphora . The data revealed that the essential oils consist primarily of monoterpenes with only very minor quantities of sesquiterpenes and diterpenes and that the essential oil differs in different chemotypes of C. camphora , with higher yields of (−)-borneol from the borneol-type than from the linalool-type. To study the terpenoid biosynthesis of signature compounds of the major monoterpenes, we performed RNA sequencing to profile the leaf transcriptomes of the two chemotypes of C. camphora . A total of 23.76 Gb clean data was generated from two chemotypes and assembled into 156,184 unigenes. The total length, average length, N50 and GC content of unigenes were 155,645,929 bp, 997 bp, 1430 bp, and 46.5%, respectively. Among them, 76,421 unigenes were annotated by publicly available databases, of which 67 candidate unigenes were identified to be involved in terpenoid biosynthesis in C. camphora . A total of 2863 unigenes were identified to be differentially expression between borneol-type and linalool-type, including 1714 up-regulated and 1149 down-regulated unigenes. Most genes encoding proteins involved in terpenoid precursor MVA and MEP pathways were expressed in similar levels in both chemotypes of C. camphora . In addition, 10 and 17 DEGs were significantly enriched in the terpene synthase activity and oxidoreductase activity terms of their directed acyclic graphs (DAG), respectively. Three monoterpene synthase genes, TPS14-like1, TPS14-like2 and TPS14-like3 were up-regulated in the borneol-type compared to the linalool-type, and their expression levels were further verified using quantitative real-time PCR. Conclusions This study provides a global overview of gene expression patterns related to terpenoid biosynthesis in C. camphora , and could contribute to a better understanding of the differential accumulation of terpenoids in different C. camphora chemotypes.

The WUSCHEL-related homeobox (WOX) gene family is integral to plant growth and development. Here, we identified 14 CcWOX genes from the Cinnamomum camphora genome and analyzed their phylogeny, conserved features, and expression patterns. Phylogenetic inference grouped CcWOX into the Ancient, Intermediate, and WUS clades, consistent with other plant lineages. Expression profiling across seven tissues/organs, together with qRT-PCR validation, revealed tissue-biased expression for several members (e.g., floral or root enrichment), suggesting gene-specific roles during development. Using AlphaFold3, we predicted monomeric structures for CcWOX proteins and an interface model compatible with an interaction between CcWOX3 and CcLBD33. Consistently, bimolecular fluorescence complementation (BiFC) in Nicotiana benthamiana detected nuclear YFP signals for cEYFP-CcWOX3 + nEYFP-CcLBD33 relative to appropriate negative controls, confirming a physical interaction in plant cells. While these findings support a putative WOX–LBD interaction module in C. camphora, the regulatory functions remain to be established. Overall, this work provides a framework for dissecting the CcWOX family in C. camphora and illustrates how AI-assisted structure prediction can be integrated with cell-based assays to accelerate hypothesis generation in plant developmental biology.

Partial discharge (PD) localization is important for monitoring and maintaining high-voltage equipment, which can help to prevent accidents. In this work, an acoustic localization sensor based on microelectromechanical system (MEMS) microphone array is proposed, which can detect and locate the partial discharge through a beam-forming algorithm. The MEMS microphone array consists of eight commercial MEMS microphones (SPV08A0LR5H-1, Knowles Electronics, IL, USA) with an aperture size of about 0.1 m × 0.1 m, allowing for a small hardware size and low cost. In order to optimize the acoustic performance of the array, a random array topology is designed. The simulation analysis indicates that the designed random topology is superior to several commonly used topologies. In terms of the localization algorithm, a deconvolution method called Fourier-based fast iterative shrinkage thresholding algorithm (FFT-FISTA) is applied. Simulation and experiment results demonstrate that FFT-FISTA used in the proposed acoustic localization sensor has significant advantages over the conventional beam-forming algorithm on spatial resolution and sidelobe suppression. Experimental results also show that the average localization error of the proposed scheme is about 0.04 m, which can meet the demands of practical application.

Background Lettuce ( Lactuca sativa L.) cultivated in facilities display low vitamin C (L-ascorbic acid (AsA)) contents which require augmentation. Although UV-B irradiation increases the accumulation of AsA in crops, processes underlying the biosynthesis as well as metabolism of AsA induced by UV-B in lettuce remain unclear. Results UV-B treatment increased the AsA content in lettuce, compared with that in the untreated control. UV-B treatment significantly increased AsA accumulation in a dose-dependent manner up until a certain dose.. Based on optimization experiments, three UV-B dose treatments, no UV-B (C), medium dose 7.2 KJ·m − 2 ·d − 1 (U1), and high dose 12.96 KJ·m − 2 ·d − 1 (U2), were selected for transcriptome sequencing (RNA-Seq) in this study. The results showed that C and U1 clustered in one category while U2 clustered in another, suggesting that the effect exerted on AsA by UV-B was dose dependent. MIOX gene in the myo-inositol pathway and APX gene in the recycling pathway in U2 were significantly different from the other two treatments, which was consistent with AsA changes seen in the three treatments, indicating that AsA accumulation caused by UV-B may be associated with these two genes in lettuce. UVR8 and HY5 were not significantly different expressed under UV-B irradiation, however, the genes involved in plant growth hormones and defence hormones significantly decreased and increased in U2, respectively, suggesting that high UV-B dose may regulate photomorphogenesis and response to stress via hormone regulatory pathways, although such regulation was independent of the UVR8 pathway. Conclusions Our results demonstrated that studying the application of UV-B irradiation may enhance our understanding of the response of plant growth and AsA metabolism-related genes to UV-B stress, with particular reference to lettuce.

Background Cinnamomum camphora is a valuable aromatic oil-producing species with significant economic and industrial importance. Linalool, a monoterpenoid compound and a major component of camphor leaf essential oil, is widely used in cosmetics, food, and pharmaceuticals. While microRNAs (miRNAs) are known to regulate terpenoid biosynthesis, their regulatory role in linalool biosynthesis remains largely unexplored. Results In this study, we performed small RNA and degradome sequencing on three C. camphora samples (H_MAR, H_MAY, and L_MAY) exhibiting significant differences in linalool content. A total of 199 known and 200 novel miRNAs were identified. Among them, 170 differentially expressed miRNAs (DEMs; 83 downregulated and 87 upregulated) were detected between H_MAY and H_MAR, whereas 77 DEMs (45 downregulated and 32 upregulated) were found between H_MAY and L_MAY. Degradome analysis predicted 223 target genes for 52 known miRNAs and 86 targets for 39 novel miRNAs. Network analysis revealed that the miRNA-SPL module may play a critical role in indirectly regulating linalool biosynthesis. Conversely, the miR167- Cca.gene21941 ( GPPS ) module may directly regulate monoterpene biosynthesis in linalool-type C. camphora . Additionally, miR5368 was found to target Cca.gene21642 (DHDDS) and Cca.gene34720 (GGDR), both of which may contribute directly to linalool biosynthesis. Conclusion These findings enhance the understanding of post-transcriptional regulation in linalool biosynthesis and provide insights for developing genetic improvement strategies for C. camphora .

Cinnamomum camphora (Lauraceae), an evergreen arborescent species endemic to East Asian ecosystems, is ecologically and economically prized for three cardinal attributes: decay-resistant xylem, aesthetic canopy architecture, and pharmacologically active terpenoid emissions. The plant-specific Lateral Organ Boundaries Domain (LBD) transcription factors mediate phylogenetically conserved developmental pathways governing lateral organogenesis and secondary metabolism across embryophytes. Despite multiple published C. camphora genome assemblies, functional characterization of LBD transcription factors in this species remains limited. We systematically identified 40 LBD genes through whole-genome analysis and characterized their structural features, evolutionary relationships, and expression patterns. Five are intron-free, while seven genes harbor two or more introns each. Detailed annotation of CcLBD promoter regions identified 33 cis-regulatory elements linked to hormone signaling and stress adaptation. Transcriptional dynamics of the 40 CcLBD genes were profiled across seven tissues of the camphor tree using short-read RNA-Seq, revealing that 22 genes were highly expressed in flowers and 12 were predominantly expressed in roots, suggesting potential roles in reproductive organ development and root formation in C. camphora . Phylogenetic analysis classified all CcLBD proteins into two clades, each harboring a conserved lateral organ boundaries (LOB) domain. Integrative omics analyses (small RNA-seq and degradome data) further implicated miR408 and miR2950c in post-transcriptional regulation of CcLBD5 via mRNA cleavage. These results establish a framework for the functional dissection of LBD-mediated developmental and stress-response pathways in C. camphora .

In this work, a miniaturized, low-cost, low-power and high-sensitivity AlN-based micro-electro-mechanical system (MEMS) hydrophone is proposed for monitoring water pipeline leaks. The proposed MEMS Hydrophone consists of a piezoelectric micromachined ultrasonic transducer (PMUT) array, an acoustic matching layer and a pre-amplifier amplifier circuit. The array has 4 (2 × 2) PMUT elements with a first-order resonant frequency of 41.58 kHz. Due to impedance matching of the acoustic matching layer and the 40 dB gain of the pre-amplifier amplifier circuit, the packaged MEMS Hydrophone has a high sound pressure sensitivity of −170 ± 2 dB (re: 1 V/μPa). The performance with respect to detecting pipeline leaks and locating leak points is demonstrated on a 31 m stainless leaking pipeline platform. The standard deviation (STD) of the hydroacoustic signal and Monitoring Index Efficiency (MIE) are extracted as features of the pipeline leak. A random forest model is trained for accurately classifying the leak and no-leak cases using the above features, and the accuracy of the model is about 97.69%. The cross-correlation method is used to locate the leak point, and the localization relative error is about 10.84% for a small leak of 12 L/min.

Waterlogging is a major abiotic stress that restricts plant growth, productivity, and survival by disrupting root aeration and altering hormonal homeostasis. To elucidate the physiological and molecular responses associated with flooding tolerance in Liriodendron hybrids (Liriodendron chinense × Liriodendron tulipifera), this study investigated its morphological, physiological, and transcriptomic changes under 0, 1, 3, and 6 days of waterlogging. Roots exhibited rapid decay, while leaves showed delayed chlorosis and reduced chlorophyll content. Changes in antioxidant enzyme activities reflected enhanced antioxidant capacity, with superoxide dismutase (SOD) activity decreasing and peroxidase (POD) and catalase (CAT) activities increasing. Hormone measurements indicated organ-specific patterns, including abscisic acid (ABA) accumulation in leaves and decreased indole-3-acetic acid (IAA) and gibberellin (GA) levels in both roots and leaves. Transcriptome profiling revealed extensive transcriptional adjustments in hormone biosynthesis, signaling, and stress-responsive pathways, including divergent regulation of ABA-associated genes in leaves and roots and broad downregulation of auxin- and gibberellin-related genes. Key ABA biosynthetic genes (NCED1, ABA2) and signaling components (PYL4, PP2C, ABF) were upregulated in leaves but downregulated in roots, whereas auxin (YUC6) and gibberellin (GA20ox) genes were generally suppressed. These coordinated physiological and molecular responses suggest organ-differentiated adaptation to waterlogging in Liriodendron hybrids, highlighting candidate pathways and genes for further investigation and providing insights for improving flooding tolerance in woody species.

Cinnamomum camphora , a member of the Lauraceae family, is a valuable aromatic and timber tree that is indigenous to the south of China and Japan. All parts of Cinnamomum camphora have secretory cells containing different volatile chemical compounds that are utilized as herbal medicines and essential oils. Here, we reported the complete sequencing of the chloroplast genome of Cinnamomum camphora using illumina technology. The chloroplast genome of Cinnamomum camphora is 152,570 bp in length and characterized by a relatively conserved quadripartite structure containing a large single copy region of 93,705 bp, a small single copy region of 19,093 bp and two inverted repeat (IR) regions of 19,886 bp. Overall, the genome contained 123 coding regions, of which 15 were repeated in the IR regions. An analysis of chloroplast sequence divergence revealed that the small single copy region was highly variable among the different genera in the Lauraceae family. A total of 40 repeat structures and 83 simple sequence repeats were detected in both the coding and non-coding regions. A phylogenetic analysis indicated that Calycanthus is most closely related to Lauraceae , both being members of Laurales , which forms a sister group to Magnoliids . The complete sequence of the chloroplast of Cinnamomum camphora will aid in in-depth taxonomical studies of the Lauraceae family in the future. The genetic sequence information will also have valuable applications for chloroplast genetic engineering.

The subtropical region of China possesses abundant broad-leaf tree species resources; however, the anatomical properties and microstructure of the wood are still unclear, which restricts the processing and utilization of wood. In this study, 14 broad-leaf trees and four coniferous trees were selected. Wood anatomical indices and wood microanatomy were used to evaluate the wood properties using a comprehensive index method. The results have shown that Dalbergia assamica exhibited the highest wood basic density among the 14 broad-leaved tree species, accompanied by a significant fiber proportion and vessel lumen diameter but a small vessel proportion and a high number of wood rays. Conversely, Parakmeria lotungensis and Michelia chapensis had relatively low wood basic densities, rendering them less suitable as valuable broad-leaved wood sources. Altingia chinensis, Castanopsis kawakamii, and the remaining 11 tree species exhibited medium-level wood basic densities. The 14 broad-leaved tree species had medium-length fibers. Phoebe bournei, Dalbergia assamica, and Castanopsis kawakamii demonstrated relatively high fiber proportion. Altingia chinensis, Dalbergia assamica, and Castanopsis kawakamii exhibited a large number of wood rays, making their wood more susceptible to cracking, whereas other broad-leaved tree species possessed fewer wood rays. The findings have provided a scientific basis for the exploration of precious broad-leaved tree resources and wood use.