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Universities are under mounting pressure to partner with societal stakeholders and organizations to collaboratively create and implement sustainability-advancing knowledge, tools, and societal transformations. Simultaneously, an increasing number of societal organizations are reaching out to partner with universities to achieve organizational objectives and increase the effectiveness of strategies to further societal sustainability. Using a conceptual framework of “sustainability co-creation”, this study empirically examines the historical and ongoing experiences of five organizations in Japan that actively partner with universities to enhance sustainability activities and strategies to transform society. We examine motivations for partnering with universities, innovative models of practice, factors hampering the co-creative potential of the university, and desired changes to overcome these. Our empirical study leads to the proposal of a typology that might assist in categorizing and understanding key attributes of differing types of sustainability co-creation. We build our typology from two perspectives: First, in terms of the primary objective of the co-creation (ranging from knowledge production to the transformation of society), and second, in terms of the approach taken (ranging from either socially or technologically-centered). We then reflect on the organizations’ experiences to offer several strategies that could increase the effectiveness of the university when partnering with stakeholders in sustainability co-creation. We also highlight several factors effecting the university’s capacity to move beyond knowledge production towards implementation measures to transform society with external stakeholders.

This book builds up on the experience and lessons learnt by academics at the Graduate Program in Sustainability Science, Global Leadership Initiative (GPSS-GLI) at the University of Tokyo. A number of scholars in the new field of sustainability science describe how field methods and exercises are carried out in this discipline, together with the theoretical basis for such exercises. Case studies of various countries around the world where these exercises are carried out are showcased, emphasizing the various socio-economic considerations and problems facing humanity and possible ways forward to build more sustainable and resilient societies. The final objective is to enrich the field of sustainability science by describing the novel aspects used in the field exercises carried out by practitioners of this cross-disciplinary field.

The world is facing a multitude of pressing problems, including environmental degradation, natural disasters, and social inequity, to name but a few. These challenges are also complex and uncertain in nature, though it is crucial for humanity to attempt to solve them in order to achieve sustainable societies. The Graduate Program in Sustainability Science-Global Leadership Initiative (GPSS-GLI) of the University of Tokyo is an academic program which looks forward to facing these challenges. The program has a strong focus on field exercises, which attempt to introduce students to the real situations being experienced by people. Students are encouraged to deal with complexity by engaging the issue from a holistic (“top-down”) and transboundary (“bottom-up”) perspective. Having a holistic view and transboundary perspective may provide a basis to deal with the complexities and uncertainties present in sustainability issues, where it is difficult to provide solutions by thinking only of fixed end-targets. Through such efforts it is hoped that students can understand and propose solutions on how to achieve more sustainable and resilience societies. The present chapter will serve as an introduction to the rest of the chapters in this book, briefly outlining the general philosophy of the GPSS-GLI regarding Global Field Exercises (GFEs) and Exercises in Resilience (ERs).

Few studies have investigated the subcellular effects of low temperature on gene expression in shrimp and most other crustaceans. Before gene expression analysis is conducted, suitable housekeeping genes (HKGs) must be confirmed to account for differences in reverse transcription process efficiency among samples. Thus, this study aimed to verify five frequently used HKGs, namely 18S ribosomal RNA (18S rRNA), ATPase, histone 3, β-actin, and glyceraldehyde 3-phosphate dehydrogenase (gapdh) for use in experiments for assessing the molecular-scale effects of cryopreservation on coral banded shrimp ( Stenopus hispidus ) embryos. To conduct chilling studies, we subjected S. hispidus  embryos to incubation at either 26 °C (control) or 5 °C for 0, 4, 8, 16, or 32 h. The software tools GeNorm, NormFinder, and Bestkeeper were employed to identify the most suitable HKG. GeNorm identified histone 3 and 18S rRNA as the most stable genes. By contrast, NormFinder determined that 18S rRNA is a stable gene for eye-formation and pre-hatch stage samples. Finally, Bestkeeper determined that gapdh and β-actin are the most suitable genes. This study is the first to identify suitable HKGs for studying shrimp embryos at low temperatures. Its findings can aid future research on evaluating the effects of cryopreservation on gene expression in crustaceans.

In existing festival research, trust is often placed at the post-experience stage by scholars, and there is no research to explore the relationship between festivalscape, trust, and experience. In addition, the main function of trust is to reduce uncertainty and risk perception. Existing festival-related research scenarios do not have typical risks, which will limit the findings of the research. This research focuses on festivals under covid-19 pandemic, which is a typical risky scenario. Exploring the antecedent variables and the consequences of the trust of the festival participants in this context may therefore make up the deficiency of existing research. Several interesting findings have been made: the perception of the festivalscape during the pandemic has been significantly simplified. Epidemic prevention measures and staff may increase trust, but trust cannot have a direct and significant impact on festival satisfaction. While staff positively strengthen trust, they will have a significant negative impact on satisfaction.

Numerous physical, chemical, and biological factors influence coral resilience in situ, yet current models aimed at forecasting coral health in response to climate change and other stressors tend to focus on temperature and coral abundance alone. To develop more robust predictions of reef coral resilience to environmental change, we trained an artificial intelligence (AI) with seawater quality, benthic survey, and molecular biomarker data from the model coral Pocillopora acuta obtained during a research expedition to the Solomon Islands. This machine-learning (ML) approach resulted in neural network models with the capacity to robustly predict (R2 = ~0.85) a benchmark for coral stress susceptibility, the “coral health index,” from significantly cheaper, easier-to-measure environmental and ecological features alone. A GUI derived from an ML desirability analysis was established to expedite the search for other climate-resilient pocilloporids within this Coral Triangle nation, and the AI specifically predicts that resilient pocilloporids are likely to be found on deeper fringing fore reefs in the eastern, more sparsely populated region of this under-studied nation. Although small in geographic expanse, we nevertheless hope to promote this first attempt at building AI-driven predictive models of coral health that accommodate not only temperature and coral abundance, but also physiological data from the corals themselves.

Given the previously documented importance of lipid concentration and composition in the successful cryopreservation of gorgonian corals, these parameters were assessed in oocytes of five species of scleractinian coral; Platygyra daedalea, Echinopora gemmacea, Echinophyllia aspera, Oxypora lacera and Astreopora expansa. Wax esters, phosphatidylethanolamine, phosphatidylcholine, and fatty acids were all measured at detectable levels, and the latter were produced at significantly elevated quantities in E. gemmacea, E. aspera, and O. lacera. On the other hand, phosphatidylethanolamine, phosphatidylcholine, and wax ester were found at significantly higher concentrations in A. expansa oocytes. Triacylglycerol was not present in any species. Interestingly, the total lipid content of oocytes from all five scleractinians was significantly lower than that of oocytes of two gorgonian species, Junceella juncea and Junceella fragilis. As higher total lipid concentrations may be correlated with greater degrees of cellular membrane fluidity at lower temperatures, it stands to reason that gorgonian coral oocytes may be more likely to survive the cryopreservation process than oocytes of scleractinian corals.

Vitrification and ultrarapid laser warming are crucial for the cryopreservation of animal embryos, oocytes, and other cells of medicinal, genetic, and agricultural value. In the present study, we focused on alignment and bonding techniques for a special cryojig that combines a jig tool and jig holder into one piece. This novel cryojig was used to obtain a high laser accuracy of 95% and a successful rewarming rate of 62%. The experimental results indicated that our refined device improved laser accuracy in the warming process after long-term cryo-storage through vitrification. We anticipate that our findings will lead to cryobanking applications that use vitrification and laser nanowarming to preserve cells and tissues from a wide range of species.

Coral reefs are suffering on a global scale due to human impacts, thereby necessitating cryopreservation efforts. The objective of this study was to develop a suitable vitrification and laser warming protocol for larvae of the scleractinian coral Seriatopora caliendrum , which inherit their dinoflagellate algal symbionts vertically. Toxicity experiments were conducted with the cryoprotectants (CPAs) ethylene glycol (EG), propylene glycol (PG), dimethyl sulfoxide (DMSO), glycerol (GLY), and methanol (METH; listed in order from least to most toxic), and larvae were subjected to vitrification and laser warming using 2 M EG + 1 M PG and 2 M EG + 1 M DMSO. Vitrification and laser warming (300 V, 10 ms pulse width, 2 mm beam diameter) using a vitrification solution of 2 M EG + 1 M PG, 40% w/v Ficoll, and 10% v/v gold nanobars (GNB) at a final concentration of 1.2 × 10 18 GNB/mL and a characteristic wavelength of 535 nm resulted in larvae with vitality and settlement percentages of 55 and 9%, respectively. This represents the first successful instance of cryopreservation of coral larvae that proceeded to settle upon warming, and suggests that the vitrification and ultra-fast laser warming approach may be applicable to other threatened marine species.