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result(s) for
"Chen, Gan-Lin"
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Functional relationship between photosynthetic leaf gas exchange in response to silicon application and water stress mitigation in sugarcane
by
Wu, Kai-Chao
,
Verma, Krishan K.
,
Li, Yang-Rui
in
Bio-modelling
,
BIOLOGY
,
Biomedical and Life Sciences
2021
Background
Water stress is one of the serious abiotic stresses that negatively influences the growth, development and production of sugarcane in arid and semi-arid regions. However, silicon (Si) has been applied as an alleviation strategy subjected to environmental stresses.
Methods
In this experiment, Si was applied as soil irrigation in sugarcane plants to understand the mitigation effect of Si against harmful impact of water stress on photosynthetic leaf gas exchange.
Results
In the present study we primarily revealed the consequences of low soil moisture content, which affect overall plant performance of sugarcane significantly. Silicon application reduced the adverse effects of water stress by improving the net photosynthetic assimilation rate (
A
net
) 1.35–18.75%, stomatal conductance to water vapour (gs) 3.26–21.57% and rate of transpiration (E) 1.16–17.83%. The mathematical models developed from the proposed hypothesis explained the functional relationships between photosynthetic responses of Si application and water stress mitigation.
Conclusions
Silicon application showed high ameliorative effects on photosynthetic responses of sugarcane to water stress and could be used for mitigating environmental stresses in other crops, too, in future.
Journal Article
Unlocking the role of silicon against biotic stress in plants
by
Liang, Qiang
,
Verma, Krishan K.
,
Xu, Lin
in
Agricultural ecosystems
,
Agricultural production
,
Agrochemicals
2024
The requirement for agricultural crops continues to enhance with the continuous growth of the human population globally. Plant pathogenic diseases outbreaks are enhancing and threatening food security and safety for the vulnerable in different regions worldwide. Silicon (Si) is considered a non-essential element for plant growth. It regulates the biological functions, plant development and productivity, and balance the defense mechanism in response to fungal, bacterial and pest attacks. The optimum crop yield can be achieved by applying Si in agricultural systems through different methods to replace or minimize the use of synthetic fertilizers. This approach can be effective on crop production during limited resources, extreme climates, pests and diseases, and environmental pollution. Silicon can be applied as foliar spray, priming of seeds, soil water irrigation, soil amendment and soilless medium (hydroponic) to enhance plant performance and stress tolerance capacity during stress conditions. This article summarized the effective roles of Si and the ability to perform in agroecosystems for better crop production, food security and safety for sustainable agriculture in the future.
Journal Article
Low-complexity detection for MIMO visible light communication system using generalized spatial modulation
2025
In this paper, the detection algorithms of two generalized spatial modulation (GSM) schemes in a multiple-input multiple-output (MIMO) visible light communication (VLC) system are developed and analyzed. The considered optical GSM schemes are GSM with Transmit Diversity (GSM-TD) and GSM with Multi-Stream (GSM-MS). By allowing each activated LED to transmit independent temporal symbols simultaneously, GSM-MS benefits from higher bits per channel use (bpcu) than the GSM-TD scheme. Existing maximum likelihood (ML) detection algorithm is computationally prohibitive; hence, we develop a two-stage (identification followed by detection) receiver that decodes the spatial and temporal symbols in a sequential manner. In the first stage, we propose two algorithms, namely subspace tracking and spatial matched filtering (SMF), to identify the indices of activated LEDs, while in the second stage, effective matched filter (EMF) and decorrelating detector are employed to extract temporal symbols for GSM-TD and GSM-MS scheme, respectively. The performance assessment, including the computation load, correct identification probability and the average symbol error rate (SER), is comprehensively evaluated by computation simulation. It is shown that the complexity of the proposed two detection algorithms is extensively reduced compared with the ML algorithm. It is also demonstrated that ML detector offers the best SER performance; nevertheless, SER of the proposed algorithms approaches ML detector in high signal-to-noise ratio (SNR) or large receiver array size scenarios.
Journal Article
Comparative Metabolomic and Transcriptomic Analyses Identify Candidate Genes Associated with Flavonoid Accumulation and Phenylpropanoid Metabolism in Large-Fruited Hawthorn ( Malus doumeri (Bois) Chev.)
by
Ran, Lu-Xia
,
Dai, Xiao-Hua
,
Wei, Xiang-Ying
in
Antioxidants
,
Biological activity
,
Biosynthesis
2026
Large-fruited hawthorn (
(Bois) Chev.) is valued for its health-promoting properties, largely attributed to its rich flavonoid content. However, little is known about the specific composition of flavonoids and the molecular mechanisms regulating their biosynthesis. The present study employed non-targeted metabolomic and transcriptomic approaches to investigate two
germplasms (G8 and G9) that exhibited significantly different total flavonoid contents. The results indicated that the major and differential metabolites primarily include flavonoids and isoflavonoids. Differentially expressed genes were significantly enriched in phenylpropanoid and flavone and flavonol biosynthesis pathways. Integrated analysis identified several structural genes and transcription factors, including
(LOC114821133, LOC103403337, LOC103454980),
(LOC103427630), and
(LOC103422512), that were significantly upregulated in the high-flavonoid genotype (G9). qRT-PCR validation confirmed the RNA-Seq expression patterns, suggesting the potential involvement of these genes in the biosynthesis of phenylpropanoid-related metabolites, such as [6]-gingerol. Applied experiments further demonstrated that freeze-drying preserved high metabolite contents and antioxidant activity. Collectively, these findings provide insights into the compositional characteristics of the major flavonoids in
and the biosynthesis of phenylpropanoid-derived metabolites. This study provides data support for future mechanistic validation and evaluation of processing technology applicability.
Journal Article
Developing mathematical model for diurnal dynamics of photosynthesis in Saccharum officinarum responsive to different irrigation and silicon application
by
Wu, Kai-Chao
,
Li, Yang Rui
,
Verma, Krishan K.
in
Abiotic stress
,
Agricultural Science
,
Biomass
2020
In the dynamic era of climate change, agricultural farming systems are facing various unprecedented problems worldwide. Drought stress is one of the serious abiotic stresses that hinder the growth potential and crop productivity. Silicon (Si) can improve crop yield by enhancing the efficiency of inputs and reducing relevant losses. As a quasi-essential element and the 2nd most abundant element in the Earth’s crust, Si is utilized by plants and applied exogenously to combat drought stress and improve plant performance by increasing physiological, cellular and molecular responses. However, the physiological mechanisms that respond to water stress are still not well defined in Saccharum officinarum plants. To the best of our knowledge, the dynamics of photosynthesis responsive to different exogenous Si levels in Saccharum officinarum has not been reported to date. The current experiment was carried out to assess the protective role of Si in plant growth and photosynthetic responses in Saccharum officinarum under water stress conditions. Saccharum officinarum cv. ‘GT 42’ plants were subjected to drought stress conditions (80–75%, 55–50% and 35–30% of soil moisture) after ten weeks of normal growth, followed by the soil irrigation of Si (0, 100, 300 and 500 mg L −1 ) for 8 weeks. The results indicated that Si addition mitigated the inhibition in Saccharum officinarum growth and photosynthesis, and improved biomass accumulation during water stress. The photosynthetic responses (photosynthesis, transpiration and stomatal conductance) were found down-regulated under water stress, and it was significantly enhanced by Si application. No phytotoxic effects were monitored even at excess (500 mg L −1 ). Soil irrigation of 300 mg L −1 of Si was more effective as 100 and 500 mg L −1 under water stress condition. It is concluded that the stress in Saccharum officinarum plants applied with Si was alleviated by improving plant fitness, photosynthetic capacity and biomass accumulation as compared with the control. Thus, this study offers new information towards the assessment of growth, biomass accumulation and physiological changes related to water stress with Si application in plants.
Journal Article
Mitigating Climate Change for Sugarcane Improvement: Role of Silicon in Alleviating Abiotic Stresses
by
Solomon, Sushil
,
Verma, Krishan K.
,
Li, Yang-Rui
in
Abiotic stress
,
Agricultural land
,
Agricultural production
2020
Climatic variables are the main factors determining the crop productivity, and these undergo changes in the course of time. Though silicon (Si) is not an essential element for the plants, its significant role has been observed in various plant species. Use of silicon could stimulate plant growth and mitigate multiple stresses such as salinity, drought, heat, extreme temperature, metal toxicity and nutritional imbalance as well as the stress linked with changes in global climatic variables. Silicon can also delay leaf senescence. In addition, Si is not harmful to plants even when present in excess. Eco-friendly techniques would be needed for addressing the growing demands for food grains and other agricultural production. The use of Si would become a sustainable strategy for increasing crop yield, improving quality and mitigating abiotic stresses in the near future. This review highlights our current understanding on the use of silicon to mitigate abiotic stresses and enhance crop production.
Journal Article
Effects of Harvesting Periods and Cultivar on the Physicochemical and Sensory Properties of Two Coffee Bean Varieties
2025
Coffee (Coffea sp.) bean variety and harvesting periods are factors that directly affect its overall quality. In this study, we investigated the effects of four different harvesting periods (December, January, February and March) on the physicochemical and sensory properties of two coffee bean Catimor varieties (7963 and T8667) planted in the same orchard. Physiological characteristics were significantly affected by the delay in harvest periods, specifically the physicochemical properties of each coffee bean variety between the periods. For the green 7963 variety, the defect rate decreased from 11.08% to 4.19% while chlorogenic acid content increased from 3.78% to 4.99% as the harvest period was delayed. The 7963 variety harvested in February and March and T8667 variety harvested in February had the best quality performance, and their cupping scores were significantly higher than those harvested in other periods. Furthermore, a high correlation was found between physical attributes (defect rate, thousand-grain weight, and green bean size), chemical components (lipids, proteins, chlorogenic acid, caffeine, and trigonelline) and cupping scores.
Journal Article
The Hawthorn (Crataegus spp.) Value Chain: An Integrated Analysis of Resource Availability, Phytochemical Characterization, and Therapeutic Applications
2026
Hawthorn is widely distributed across China, including Shanxi, Henan, Hebei, Shandong, and Shaanxi provinces. It is rich in functional components and nutritional elements, making it a crucial raw material for medicinal and food products. This review provides comprehensive information of the distribution of hawthorn germplasm resources in China and compares the differences in nutrient composition, chemical substances, and functional activities among different species. Furthermore, it offers a statistical analysis of the diversified processing and applications of hawthorn in China. Finally, the review identifies current challenges in the agro-food industries and states the future outlook of the industry. By systematically integrating research findings into a comprehensive “resource–characterization–application” framework, the study addresses the current fragmentation and lack of systematic organization in hawthorn research. It seeks to provide a scientific basis for directional breeding, strategic planning of production areas, precise product development, and high-quality development of the hawthorn industry in years to come.
Journal Article
Application of Enzyme Engineering and Synthetic Biology for Modulated Transformation of Fructooligosaccharides (FOSs) to Elucidate the Catalytic Mechanism of Fructofuranosidases
2026
Fructooligosaccharides (FOSs) are plant-based prebiotics widely utilized in the food and pharmaceutical industries. As a major sugar-producing region, Guangxi holds significant potential for enzymatic production of FOS from sucrose. This study engineered a mutant enzyme, 142P-242K, to address the low catalytic activity characteristic of wild-type enzymes. The mutation upregulated the FOS conversion efficiency from 29 to 52%, respectively. Optimal enzymatic activity was observed at 45 °C, pH 6.0, and in the presence of 1 mM Na+. Mechanistic investigations revealed that modifications to the catalytic domain pocket and shifts in substrate affinity were the primary factors driving enhanced FOS production. The accumulation of 1-Kestose (GF2) was attributed to the enhanced flexibility of the 142P-242K loop, which facilitates substrate access to the active site. However, the synthesis of nystose (GF3) from GF2 is hindered by the hydrophobic nature of the active site and strong hydrogen bonds binding GF2. Comparing the enzyme’s ability to produce FOS using sugarcane juice, sugarcane molasses, and adsorption-heating sugarcane molasses, it was determined that heat-adsorbed molasses yielded the highest FOS concentration (30.77%). This study offers a practical and cost-effective strategy for enzyme modification and efficient valorization of molasses.
Journal Article
Application of Microbial Fermentation in Caffeine Degradation and Flavor Modulation of Coffee Beans
2025
Coffee is one of the most widely consumed beverages worldwide, primarily due to the stimulating effects attributed to its caffeine content. However, excessive intake of caffeine results in negative effects, including palpitations, anxiety, and insomnia. Therefore, low-caffeine coffee has captivated growing consumer interest, highlighting its significant market potential. Traditional decaffeination methods often lead to non-selective extraction, resulting in a loss of desirable flavor compounds, thereby compromising coffee quality. In recent years, microbial fermentation has emerged as a promising, targeted, and safe approach for reducing caffeine content during processing. Additionally, mixed-culture fermentation further enhances coffee flavor and overcomes the drawbacks of monoculture fermentation, such as low efficiency and limited flavor profiles. Nonetheless, several challenges are yet to be resolved, including microbial tolerance to caffeine and related alkaloids, the safety of fermentation products, and elucidation of the underlying mechanisms behind microbial synergy in co-cultures. This review outlines the variety of microorganisms with the potential to degrade caffeine and the biochemical processes involved in this process. It explores how microbes tolerate caffeine, the safety of metabolites produced during fermentation, and the synergistic effects of mixed microbial cultures on the modulation of coffee flavor compounds, including esters and carbonyls. Future directions are discussed, including the screening of alkaloid-tolerant strains, constructing microbial consortia for simultaneous caffeine degradation for flavor enhancement, and developing high-quality low-caffeine coffee.
Journal Article