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Transcriptome-wide survey divulged a total of 181 ABC transporters in G. glabra which were phylogenetically classified into six subfamilies. Protein–Protein interactions revealed nine putative GgABCBs (-B6, -B14, -B15, -B25, -B26, -B31, -B40, -B42 &-B44) corresponding to five AtABCs orthologs (-B1, -B4, -B11, -B19, &-B21). Significant transcript accumulation of ABCB6 (31.8 folds), - B14 (147.5 folds), - B15 (17 folds), -B25 (19.7 folds), - B26 (18.31 folds), -B31 (61.89 folds), -B40 (1273 folds) and - B42 (51 folds) was observed under the influence of auxin. Auxin transport-specific inhibitor, N-1-naphthylphthalamic acid, showed its effectiveness only at higher (10 µM) concentration where it down regulated the expression of ABCBs , PINs (PIN FORMED) and TWD1 (TWISTED DWARF 1) genes in shoot tissues, while their expression was seen to enhance in the root tissues. Further, qRT-PCR analysis under various growth conditions ( in-vitro , field and growth chamber), and subjected to abiotic stresses revealed differential expression implicating role of ABCBs in stress management. Seven of the nine genes were shown to be involved in the stress physiology of the plant. GgABCB6 , 15 , 25 and ABCB31 were induced in multiple stresses, while GgABCB26 , 40 & 42 were exclusively triggered under drought stress. No study pertaining to the ABC transporters from G. glabra is available till date. The present investigation will give an insight to auxin transportation which has been found to be associated with plant growth architecture; the knowledge will help to understand the association between auxin transportation and plant responses under the influence of various conditions.

Main conclusion ABCs transport diverse compounds; with plant’s most abundant ABCG and ABCB subfamilies. ABCBs are multi-functional transporter proteins having role in plant adaptation. ATP-binding cassette (ABC) proteins have been known for the transportation of various structurally diverse compounds in all kingdoms of life. Plants possess a particularly high number of ABC transporters compared to other eukaryotes: the most abundant being ABCG followed by the ABCB subfamilies. While members of the ABCB subfamily are primarily known for auxin transportation, however, studies have shown their involvement in variety of other functions viz . growth and development, biotic and abiotic stresses, metal toxicity and homeostasis, cellular redox state stability, stomatal regulation, cell shape maintenance, and transport of secondary metabolites and phytohormones. These proteins are able to perform various biological processes due to their widespread localization in the plasma membrane, mitochondrial membrane, chloroplast, and tonoplast facilitating membrane transport influenced by various environmental and biological cues. The current review compiles published insights into the role of ABCB transporters, and also provides brief insights into the role of ABCB transporters in a medicinal plant, where the synthesis of its bioactive secondary metabolite is linked to the primary function of ABCBs, i.e., auxin transport. The review discusses ABCB subfamily members as multi-functional protein and comprehensively examines their role in various biological processes that help plants to survive under unfavorable environmental conditions. Graphical abstract