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Background Aucubin (Au), an iridoid glycoside from natural plants, has antioxidative and anti-inflammatory bioactivities; however, its effects on a traumatic brain injury (TBI) model remain unknown. We explored the potential role of Au in an H 2 O 2 -induced oxidant damage in primary cortical neurons and weight-drop induced-TBI in a mouse model. Methods In vitro experiments, the various concentrations of Au (50 μg/ml, 100 μg/ml, or 200 μg/ml) were added in culture medium at 0 h and 6 h after neurons stimulated by H 2 O 2 (100 μM). After exposed for 12 h, neurons were collected for western blot (WB), immunofluorescence, and M29,79-dichlorodihydrofluorescein diacetate (DCFH-DA) staining. In vivo experiments, Au (20 mg/kg or 40 mg/kg) was administrated intraperitoneally at 30 min, 12 h, 24 h, and 48 h after modeling. Brain water content, neurological deficits, and cognitive functions were measured at specific time, respectively. Cortical tissue around focal trauma was collected for WB, TdT-mediated dUTP Nick-End Labeling (TUNEL) staining, Nissl staining, quantitative real time polymerase chain reaction (q-PCR), immunofluorescence/immunohistochemistry, and enzyme linked immunosorbent assay (ELISA) at 72 h after TBI. RNA interference experiments were performed to determine the effects of nuclear factor erythroid-2 related factor 2 (Nrf2) on TBI mice with Au (40 mg/kg) treatment. Mice were intracerebroventricularly administrated with lentivirus at 72 h before TBI establishment. The cortex was obtained at 72 h after TBI and used for WB and q-PCR. Results Au enhanced the translocation of Nrf2 into the nucleus, activated antioxidant enzymes, suppressed excessive generation of reactive oxygen species (ROS), and reduced cell apoptosis both in vitro and vivo experiments. In the mice model of TBI, Au markedly attenuated brain edema, histological damages, and improved neurological and cognitive deficits. Au significantly suppressed high mobility group box 1 (HMGB1)-mediated aseptic inflammation. Nrf2 knockdown in TBI mice blunted the antioxidant and anti-inflammatory neuroprotective effects of the Au. Conclusions Taken together, our data suggest that Au provides a neuroprotective effect in TBI mice model by inhibiting oxidative stress and inflammatory responses; the mechanisms involve triggering Nrf2-induced antioxidant system.

Primordial follicle assembly in the mouse occurs during perinatal ages and largely determines the ovarian reserve that will be available to support the reproductive life span. The development of primordial follicles is controlled by a complex network of interactions between oocytes and ovarian somatic cells that remain poorly understood. In the present research, using single-cell RNA sequencing performed over a time series on murine ovaries, coupled with several bioinformatics analyses, the complete dynamic genetic programs of germ and granulosa cells from E16.5 to postnatal day (PD) 3 were reported. Along with confirming the previously reported expression of genes by germ cells and granulosa cells, our analyses identified 5 distinct cell clusters associated with germ cells and 6 with granulosa cells. Consequently, several new genes expressed at significant levels at each investigated stage were assigned. By building single-cell pseudotemporal trajectories, 3 states and 1 branch point of fate transition for the germ cells were revealed, as well as for the granulosa cells. Moreover, Gene Ontology (GO) term enrichment enabled identification of the biological process most represented in germ cells and granulosa cells or common to both cell types at each specific stage, and the interactions of germ cells and granulosa cells basing on known and novel pathway were presented. Finally, by using single-cell regulatory network inference and clustering (SCENIC) algorithm, we were able to establish a network of regulons that can be postulated as likely candidates for sustaining germ cell-specific transcription programs throughout the period of investigation. Above all, this study provides the whole transcriptome landscape of ovarian cells and unearths new insights during primordial follicle assembly in mice.

Background: Non-alcoholic fatty liver disease (NAFLD) is a chronic advanced liver disease that is highly related to metabolic disorders and induced by a high-fat diet (HFD). Recently, epigallocatechin gallate (EGCG) has been regarded as a protective bioactive polyphenol in green tea that has the ability to protect against non-alcoholic fatty liver disease, but the molecular mechanism remains poorly deciphered. Ferroptosis plays a vital role in the progression of non-alcoholic fatty liver disease, but experimental evidence of ferroptosis inhibition by epigallocatechin gallate is limited. Hence, our study aimed to investigate the effect and mechanisms of epigallocatechin gallate on hepatic ferroptosis to mitigate hepatic injury in high-fat diet-fed mice. Methods: Fifty male C57BL/6 mice were fed either a standard chow diet (SCD), a high-fat diet, or a high-fat diet and administered epigallocatechin gallate or ferrostatin-1 (a ferroptosis-specific inhibitor) for 12 weeks. Liver injury, lipid accumulation, hepatic steatosis, oxidative stress, iron overload, and ferroptosis marker proteins were examined. In vitro , steatotic L-02 cells were used to explore the underlying mechanism. Results: In our research, we found that epigallocatechin gallate notably alleviated liver injury and lipid accumulation, oxidative stress, hepatic steatosis, decreased iron overload and inhibited ferroptosis in a high-fat diet-induced murine model of non-alcoholic fatty liver disease. In vitro experiments, using ferrostatin-1 and a mitochondrial reactive oxygen species (MtROS) scavenger (Mito-TEMPO), we found that epigallocatechin gallate remarkably alleviated oxidative stress and inhibited ferroptosis by reducing the level of mitochondrial reactive oxygen species in steatotic L-02 cells. Conclusion: Taken together, our results revealed that epigallocatechin gallate may exert protective effects on hepatic lipotoxicity by inhibiting mitochondrial reactive oxygen species-mediated hepatic ferroptosis. Findings from our study provide new insight into prevention and treatment strategies for non-alcoholic fatty liver disease pathological processes.

This paper constructs a two-layer road data asset revenue allocation model based on a modified Shapley value approach. The first layer allocates revenue to three roles in the data value realization process: the original data collectors, the data processors, and the data product producers. It fully considers and appropriately adjusts the revenue allocation to each role based on data risk factors. The second layer determines the correction factors for different roles to distribute revenue among the participants within those roles. Finally, the revenue values of the participants within each role are synthesized to obtain a consolidated revenue distribution for each participant. Compared to the traditional Shapley value method, this model establishes a revenue allocation evaluation index system, uses entropy weighting and rough set theory to determine the weights, and adopts a fuzzy comprehensive evaluation and numerical analysis to assess the degree of contribution of participants. It fully accounts for differences in both the qualitative and quantitative contributions of participants, enabling a fairer and more reasonable distribution of revenues. This study provides new perspectives and methodologies for the benefit distribution mechanism in road data assets, which aid in promoting the market-based use of road data assets, and it serves as an important reference for the application of data assetization in the road transportation industry.

DNA methylation is a key epigenetic modification in mammals and plays important roles in muscle development. We sampled longissimus dorsi muscle (LDM) from a well-known elite native breed of Chinese Qinchuan cattle living within the same environment but displaying distinct skeletal muscle at the fetal and adult stages. We generated and provided a genome-wide landscape of DNA methylomes and their relationship with mRNA and miRNA for fetal and adult muscle studies. Integration analysis revealed a total of 77 and 1,054 negatively correlated genes with methylation in the promoter and gene body regions, respectively, in both the fetal and adult bovine libraries. Furthermore, we identified expression patterns of high-read genes that exhibit a negative correlation between methylation and expression from nine different tissues at multiple developmental stages of bovine muscle-related tissue or organs. In addition, we validated the MeDIP-Seq results by bisulfite sequencing PCR (BSP) in some of the differentially methylated promoters. Together, these results provide valuable data for future biomedical research and genomic and epigenomic studies of bovine skeletal muscle that may help uncover the molecular basis underlying economically valuable traits in cattle. This comprehensive map also provides a solid basis for exploring the epigenetic mechanisms of muscle growth and development.

Background Fuzhong buffalo, a native breed of Guangxi Zhuang Autonomous Region, is traditionally used as a draft animal to provide farm power in the rice cultivation. In addition, the Fuzhong buffalo also prepared for the bullfighting festival organized by the locals. The detection of the selective signatures in its genome can help in elucidating the selection mechanisms in its stamina and muscle development of a draft animal. Results In this study, we analyzed 27 whole genomes of buffalo (including 15 Fuzhong buffalo genomes and 12 published buffalo genomes from Upper Yangtze region). The ZHp, ZFst, π-Ratio, and XP-EHH statistics were used to identify the candidate signatures of positive selection in Fuzhong buffalo. Our results detected a set of candidate genes involving in the pathways and GO terms associated with the response to exercise (e.g., ALDOA , STAT3 , AKT2 , EIF4E2 , CACNA2D2 , TCF4 , CDH2 ), immunity (e.g., PTPN22 , NKX2-3 , PIK3R1 , ITK , TMEM173 ), nervous system (e.g., PTPN21 , ROBO1 , HOMER1 , MAGI2 , SLC1A3 , NRG3 , SNAP47 , CTNNA2 , ADGRL3 ). In addition, we also identified several genes related to production and growth traits (e.g., PHLPP1 , PRKN , MACF1 , UCN3, RALGAPA1 , PHKB , PKD1L ). Our results depicted several pathways, GO terms, and candidate genes to be associated with response to exercise, immunity, nervous system, and growth traits. Conclusions The selective sweep analysis of the Fuzhong buffalo demonstrated positive selection pressure on potential target genes involved in behavior, immunity, and growth traits, etc. Our findings provided a valuable resource for future research on buffalo breeding and an insight into the mechanisms of artificial selection.

Despite the increasing use of mixed ownership (domestic and foreign shareholders) in Chinese state-owned enterprises (SOEs), the role and performance of foreign ownership is still a controversial topic. In this paper we empirically examine the effects of foreign shareholders’ social responsibility on the international competitiveness of SOEs and the moderating effect of R&D innovation. Drawing on a sample of Chinese listed SOEs with international market operations over the period 2011–2019, we find empirical evidence supporting that foreign shareholders’ social responsibility is effective in enhancing the international competitiveness of Chinese SOEs. We also find that R&D innovation strengthens the effect between foreign shareholders’ social responsibility and enterprises’ international competitiveness. These findings are valid after controlling for robustness and endogeneity factors. Furthermore, we find that the promoting effect of foreign shareholders’ social responsibility on Chinese SOEs’ international competitiveness, as well as the moderating effect of R&D innovation, is more pronounced among Chinese local SOEs, business SOEs, and manufacturing SOEs. The research in this paper contributes to the current debate and deepens our understanding regarding the role and performance of foreign shareholders in the process of mixed-ownership reform.

Land use is an embodiment of human socio-economic activities and represents a bridge between these activities and natural systems. Rural residential land represents a space for rural residents to reside in and exhibits spatial characteristics that evolve over time, which is proof of rural socio-economic development. As one of the most developed regions in China, cities along the Beijing–Hangzhou Canal experienced significant changes in rural residential land use from 1990 to 2020. This paper analyses the spatial differentiation of rural residential land in 21 cities on the Grand Canal. Then, it explores the driving factors of this land using spatial grid analysis and the geographic detector model. According to the spatial differentiation characteristics and the driving factors of rural residential land, the study proposed an improved potential model for rural residential land improvement. Lastly, it proposes three different forms of rural residential land based on the results. The study found that (1) the change in rural residential land in the northern part of the Grand Canal was more volatile than that in the southern part. The change in rural residential land from 1990 to 2020 conformed to the pattern of cultivated land–rural residential land–urban construction land. (2) Based on the driving factors of rural residential land, the land is divided into one-dimensional cities, two-dimensional cities, and three-dimensional cities. Circular, linear, and scattered cities of different sizes were affected by socio-economic factors, transportation accessibility, and the natural environment, respectively. (3) Based on the potential scale of rural residential land consolidation, different types of development strategies were proposed through research, including constructing large-scale villages, relocating and reconstructing new villages, and constructing high-quality villages, respectively. Enhancing the scientific planning of rural residential land and its efficiency and tapping into the potential of land consolidation can offer the protection of agricultural land and the integration of urban and rural areas in the new era.

Abiotic stresses such as drought, extreme temperature, and salinity can negatively impact seed germination and plant growth and have become major limitations to crop production. Most crops are vulnerable to abiotic stress factors during their early growth phase, especially during seed germination and seedling emergence. Rapid crop seed germination and seedling establishment is known to provide competitive advantages over weeds and improve yields. Seed osmopriming is defined as a pre-sowing treatment in which seeds are soaked in osmotic solutions to undergo the first stage of germination, but radicle protrusion has not occurred. The process of osmopriming involves prior exposure of seeds in low-water-potential solutions. Osmopriming can generate a series of pre-germination metabolic activities, increase the antioxidant system activities, and prepare the seed for radicle protrusion. Polyethylene glycol (PEG) is a popular osmopriming agent that can alleviate the negative impacts of abiotic stresses. This review summarizes research findings on crop responses to seed priming with PEG under abiotic stresses. The challenges, limitations, and opportunities of using PEG for crop seed priming are discussed with the goal of providing insights into future research towards effective application of seed priming in crop production.

Understanding the long-term dynamics and driving factors behind small and micro wetlands is critical for their management and future sustainability. This study explored the impacts of natural and anthropogenic factors on the spatiotemporal evolution of these areas in Wuxi area using the support vector machine (SVM) classification method and the geographic detector model based on Landsat satellite image data from 1985 to 2020. The results revealed that: (1) Natural wetlands were prominent in Wuxi area, with an average proportion of 70%, and although they exhibited a downward trend over the last ten years, the scale of natural small and micro wetlands increased 1.5-fold—from 4349.59 hm2 in 1985 to 10,841.59 hm2 in 2020. (2) The small and micro wetlands in Wuxi area had obvious seasonal variations, with most being 0.1–1 hm2 and 1–3 hm2, respectively. From the perspective of spatial distribution, they were primarily distributed in Yixing district, which accounts for 34% of Wuxi area. (3) The distribution of small and micro wetlands was systematically affected by natural and human activities. The main factors that affected the distribution of small and micro wetlands were the average annual temperature and GDP, with the interactions between all factors being nonlinear and bi-linear. The influences of natural factors on small and micro wetlands were weakened, with human activities steadily emerging as the dominant factor that affected their distribution. The results of this study can provide supportive data and a scientific basis for the ecological restoration and protection of wetlands.