Explore the vast range of titles available.

We discuss redox regulation of retrograde and epigenetic mechanisms controlling gene expression under complex environmental situations and the generation of specific ROS signatures as a key feature of cross-tolerance acquisition. Abstract Retrograde pathways occurring between chloroplasts, mitochondria, and the nucleus involve oxidative and antioxidative signals that, working in a synergistic or antagonistic mode, control the expression of specific patterns of genes following stress perception. Increasing evidence also underlines the relevance of mitochondrion-chloroplast-nucleus crosstalk in modulating the whole cellular redox metabolism by a controlled and integrated flux of information. Plants can maintain the acquired tolerance by a stress memory, also operating at the transgenerational level, via epigenetic and miRNA-based mechanisms controlling gene expression. Data discussed in this review strengthen the idea that ROS, redox signals, and shifts in cellular redox balance permeate the signalling network leading to cross-tolerance. The identification of specific ROS/antioxidative signatures leading a plant to different fates under stress is pivotal for identifying strategies to monitor and increase plant fitness in a changing environment. This review provides an update of the plant redox signalling network implicated in stress responses, in particular in cross-tolerance acquisition. The interplay between reactive oxygen species (ROS), ROS-derived signals, and antioxidative pathways is also discussed in terms of plant acclimation to stress in the short and long term.

Plants are primary resources for oxygen and foods whose production is fundamental for our life. However, diseases and pests may interfere with plant growth and cause a significant reduction of both the quality and quantity of agriculture products. Increasing agricultural productivity is crucial for poverty reduction and food security improvements. For this reason, the 2030 Agenda for Sustainable Development gives a central role to agriculture by promoting a strong technological innovation for advancing sustainable practices at the plant level. To accomplish this aim, recently, wearable sensors and flexible electronics have been extended from humans to plants for measuring elongation, microclimate, and stressing factors that may affect the plant’s healthy growth. Unexpectedly, fiber Bragg gratings (FBGs), which are very popular in health monitoring applications ranging from civil infrastructures to the human body, are still overlooked for the agriculture sector. In this work, for the first time, plant wearables based on FBG technology are proposed for the continuous and simultaneous monitoring of plant growth and environmental parameters (i.e., temperature and humidity) in real settings. The promising results demonstrated the feasibility of FBG-based sensors to work in real situations by holding the promise to advance continuous and accurate plant health growth monitoring techniques.

Wearable devices are widely spreading in various scenarios for monitoring different parameters related to human and recently plant health. In the context of precision agriculture, wearables have proven to be a valuable alternative to traditional measurement methods for quantitatively monitoring plant development. This study proposed a multi-sensor wearable platform for monitoring the growth of plant organs (i.e., stem and fruit) and microclimate (i.e., environmental temperature—T and relative humidity—RH). The platform consists of a custom flexible strain sensor for monitoring growth when mounted on a plant and a commercial sensing unit for monitoring T and RH values of the plant surrounding. A different shape was conferred to the strain sensor according to the plant organs to be engineered. A dumbbell shape was chosen for the stem while a ring shape for the fruit. A metrological characterization was carried out to investigate the strain sensitivity of the proposed flexible sensors and then preliminary tests were performed in both indoor and outdoor scenarios to assess the platform performance. The promising results suggest that the proposed system can be considered one of the first attempts to design wearable and portable systems tailored to the specific plant organ with the potential to be used for future applications in the coming era of digital farms and precision agriculture.

Plant biodiversity offers a valuable source of bioactive molecules to address critical global challenges including antimicrobial resistance (AMR), a major health threat. Wild edible plants (WEPs) have recently gained attention for their ability to accumulate specialized metabolites that are emerging for their efficacy against AMR. Although many studies suggest their potential use in combating infectious diseases, knowledge about the biochemical properties of these plants, their chemical profile and antibacterial activities, remains highly limited. In this scenery, the aim of this study was a bioprospecting of the chemical and antioxidant profile, the antibiofilm and bactericidal properties of six WEPs, largely distributed in Italy and historically used as food, namely: Silene alba , Silene vulgaris , Chenopodium album , Sonchus oleraceus, Glechoma hederacea and Diplotaxis erucoides . We applied an integrated approach, combining analytical chemistry, plant biochemistry and microbiology. These WEPs revealed notable antibiofilm and bactericidal abilities, anti-adherence and cell wall damage properties. These activities were strongly linked to the presence of phenolic compounds and to the antioxidant abilities of these plants. S. alba , S. oleraceus , and G. hederacea showed the highest efficacy. Our findings might encourage their consumption or use, which could improve dietary plant biodiversity, human health, and fight the rise of AMR.

Defined by the World Health Organization (WHO) as indigenous knowledge and practices used for maintaining health and treating illnesses, traditional medicine (TM) represents a rich reservoir of ancient healing practices rooted in cultural traditions and accumulated wisdom over centuries. Five indigenous Kenyan plant species traditionally used in African TM, named Afzelia quanzensis , Azadirachta indica , Gigasiphon macrosiphon , Grewia bicolor , and Lannea schweinfurthii , represent a valuable resource in healing practices, yet their chemical composition and bioactivity remain understudied. To depict a primary bio-chemical characterization of these plants, their antioxidant and antimicrobial features have been evaluated by the use of methods validated in this context. G. bicolor , and G. macrosiphon were found to have great potential as sources of bioactive metabolites, such as chlorophyll a (1456.29 µg/ g DW; 1104.33 µg/ g DW), chlorophyll b (712.48 µg/ g DW; 443.31 µg/ g DW), and carotenoids (369.71 µg/ g DW; 300 µg/ g DW) as well as phenols (31.78 mg GAE/g DW; 27.54 GAE/g DW), and exhibiting high antioxidant activity, according to TEAC, DPPH and FRAP assays. Additionally, L. schweinfurthii and G. macrosiphon demonstrated antimicrobial activity against the Gram-negative bacteria E. coli , as well as against Gram-positive ones, S. aureus and B. subtilis .

Global climate change and exponential population growth pose a challenge to agricultural outputs. In this scenario, novel techniques have been proposed to improve plant growth and increase crop yields. Wearable sensors are emerging as promising tools for the non-invasive monitoring of plant physiological and microclimate parameters. Features of plant wearables, such as easy anchorage to different organs, compliance with natural surfaces, high flexibility, and biocompatibility, allow for the detection of growth without impacting the plant functions. This work proposed two wearable sensors based on fiber Bragg gratings (FBGs) within silicone matrices. The use of FBGs is motivated by their high sensitivity, multiplexing capacities, and chemical inertia. Firstly, we focused on the design and the fabrication of two plant wearables with different matrix shapes tailored to specific plant organs (i.e., tobacco stem and melon fruit). Then, we described the sensors’ metrological properties to investigate the sensitivity to strain and the influence of environmental factors, such as temperature and humidity, on the sensors’ performance. Finally, we performed experimental tests to preliminary assess the capability of the proposed sensors to monitor dimensional changes of plants in both laboratory and open field settings. The promising results will foster key actions to improve the use of this innovative technology in smart agriculture applications for increasing crop products quality, agricultural efficiency, and profits.

The safeguarding of plant health is vital for optimizing crop growth practices, especially in the face of the biggest challenges of our generation, namely the environmental crisis and the dramatic changes in the climate. Among the many innovative tools developed to address these issues, wearable sensors have recently been proposed for monitoring plant growth and microclimates in a sustainable manner. These systems are composed of flexible matrices with embedded sensing elements, showing promise in revolutionizing plant monitoring without being intrusive. Despite their potential benefits, concerns arise regarding the effects of the long-term coexistence of these devices with the plant surface. Surprisingly, a systematic analysis of their influence on plant physiology is lacking. This study aims to investigate the effect of the color and geometric features of flexible matrices on two key plant physiological functions: photosynthesis and transpiration. Our findings indicate that the negative effects associated with colored substrates, as identified in recent research, can be minimized by holing the matrix surface with a percentage of voids of 15.7%. This approach mitigates interference with light absorption and reduces water loss to a negligible extent, making our work one of the first pioneering efforts in understanding the intricate relationship between plant wearables’ features and plant health.

Plants are continuously faced with complex environmental conditions which can affect the oxidative metabolism and photosynthetic efficiency, thus leading to the over-production of reactive oxygen species (ROS). Over a certain threshold, ROS can damage DNA. DNA damage, unless repaired, can affect genome stability, thus interfering with cell survival and severely reducing crop productivity. A complex network of pathways involved in DNA damage response (DDR) needs to be activated in order to maintain genome integrity. The expression of specific genes belonging to these pathways can be used as indicators of oxidative DNA damage and effective DNA repair in plants subjected to stress conditions. Managing ROS levels by modulating their production and scavenging systems shifts the role of these compounds from toxic molecules to key messengers involved in plant tolerance acquisition. Oxidative and anti-oxidative signals normally move among the different cell compartments, including the nucleus, cytosol, and organelles. Nuclei are dynamically equipped with different redox systems, such as glutathione (GSH), thiol reductases, and redox regulated transcription factors (TFs). The nuclear redox network participates in the regulation of the DNA metabolism, in terms of transcriptional events, replication, and repair mechanisms. This mainly occurs through redox-dependent regulatory mechanisms comprising redox buffering and post-translational modifications, such as the thiol-disulphide switch, glutathionylation, and S-nitrosylation. The regulatory role of microRNAs (miRNAs) is also emerging for the maintenance of genome stability and the modulation of antioxidative machinery under adverse environmental conditions. In fact, redox systems and DDR pathways can be controlled at a post-transcriptional level by miRNAs. This review reports on the interconnections between the DDR pathways and redox balancing systems. It presents a new dynamic picture by taking into account the shared regulatory mechanism mediated by miRNAs in plant defense responses to stress.

The seed habit is the most complex and successful method of sexual reproduction in vascular plants. It represents a remarkable moment in the evolution of plants that afterward spread on land. In particular, seed size had a pivotal role in evolutionary success and agronomic traits, especially in the field of crop domestication. Given that crop seeds constitute one of the primary products for consumption, it follows that seed size represents a fundamental determinant of crop yield. This adaptative feature is strictly controlled by genetic traits from both maternal and zygotic tissues, although seed development and growth are also affected by environmental cues. Despite being a highly exploited topic for both basic and applied research, there are still many issues to be elucidated for developmental biology as well as for agronomic science. This review addresses a number of open questions related to cues that influence seed growth and size and how they influence seed germination. Moreover, new insights on the genetic–molecular control of this adaptive trait are presented.

Knowledge of the stress-induced metabolic alterations in tolerant and sensitive plants is pivotal for identifying interesting traits that improve plant resilience toward unfavorable environmental conditions. This represents a hot topic area of plant science, particularly for crops, due to its implication in food security. Two rice varieties showing dissimilar resistance to salt, Baldo and Vialone Nano, have been studied to investigate the mechanisms underpinning tolerance toward salinity, and these studies have focused on the root system. A detailed analysis of the salt stress-dependent modulation of the redox network is here presented. The different phenotype observed after salt exposure in the two rice varieties is coherent with a differential regulation of cell-cycle progression and cell-death patterns observed at root level. Baldo, the tolerant variety, already showed a highly responsive antioxidative capacity in control conditions. Consistently, stressed Baldo plants showed a different pattern of H2O2 accumulation compared to Vialone Nano. Moreover, glutathione metabolism was finely modulated at transcriptional, post-transcriptional, and post-translational levels in Baldo. These results contribute to highlight the role of ROS and antioxidative pathways as a part of a complex redox network activated in rice toward salt stress.