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This is a practical guide to mutation breeding in oil palm, representing completely novel work supported by the Plant Breeding and Genetics Section of the Joint FAO/IAEA Division (Vienna, Austria). Oil palm is the top oil crop and the only major crop and only oil crop not to have been improved by plant mutation breeding. The manual is hands-on, providing step-by-step illustrated methods in mutation induction, mutation detection and mutant line development for oil palm improvement.Presenting sound practices based on scientific innovation and knowledge, this guide provides techniques integrated with expertise and is authored by practitioners actively engaged in oil palm seed production and breeding. Promoting green, eco-friendly agriculture, this book features coverage of:Radio-sensitivity testingChallenges and opportunities for mutation breedingProtocol for developing mutant generations for mutant screeningServices in irradiation treatmentsThe only available resource containing protocols and guidelines on how oil palm can be manipulated for mutation breeding, this book is essential reading for oil palm breeders, seed producers and plantation companies, oil palm traders, students and research institutes across the world. It provides a resource for training, a knowledge base for people new to oil palm and a reference guide for managers, to ensure best practices in maximising sustainability and production of this important crop..

Plant growth and development are highly regulated processes that are coordinated by hormones including the brassinosteroids (BRs), a group of steroids with structural similarity to steroid hormones of mammals. Although it is well understood how BRs are produced and how their signals are transduced, BR targets, which directly confer the hormone’s growth-promoting effects, have remained largely elusive. Here, we show that BRs regulate the biosynthesis of gibberellins (GAs), another class of growth-promoting hormones, in Arabidopsis thaliana. We reveal that Arabidopsis mutants deficient in BR signaling are severely impaired in the production of bioactive GA, which is correlated with defective GA biosynthetic gene expression. Expression of the key GA biosynthesis gene GA20ox1 in the BR signaling mutant bri1-301 rescues many of its developmental defects. We provide evidence that supports a model in which the BR-regulated transcription factor BES1 binds to a regulatory element in promoters of GA biosynthesis genes in a BR-induced manner to control their expression. In summary, our study underscores a role of BRs as master regulators of GA biosynthesis and shows that this function is of major relevance for the growth and development of vascular plants.