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Sulfur isotope composition of organic tissues is a commonly used tool for gathering information about provenance and diet in archaeology and paleoecology. However, the lack of maps predicting sulfur isotope variations on the landscape limits the possibility to use this isotopic system in quantitative geographic assignments. We compiled a database of 2,680 sulfur isotope analyses in the collagen of archaeological human and animal teeth from 221 individual locations across Western Europe. We used this isotopic compilation and remote sensing data to apply a multivariate machine-learning regression, and to predict sulfur isotope variations across Western Europe. The resulting model shows that sulfur isotope patterns are highly predictable, with 65% of sulfur isotope variations explained using only 4 variables representing marine sulfate deposition and local geological conditions. We used this novel sulfur isoscape and existing strontium and oxygen isoscapes of Western Europe to apply triple isotopes continuous-surface probabilistic geographic assignments to assess the origin of a series of teeth from local animals and humans from Brittany. We accurately and precisely constrained the origin of these individuals to limited regions of Brittany. This approach is broadly transferable to studies in archaeology and paleoecology as illustrated in a companion paper (Colleter et al. 2021).

A preventive excavation performed in 2018 prior to development work led to the discovery of more than 213 subjects buried from the 4th to the 11th centuries in the 1850 m2 dug area. This is a cemetery located in Olonne-sur-Mer in France (46.53723, −1.77603). The complex is limited to the south by a ditch. To the north, no limits were observed during the excavation and, to the west, ancient archaeological surveys suggest an extension of the burial area. Biological analysis of the skeletons reveals a demographic characterizing a natural community, with an under-representation of children under 5 and with subjects under 20 appearing to be grouped together in the center of the area. The place where the youngest are buried often testifies to a strategic position in Christian contexts (near church doors, under sub stillicidio gutters, etc.). Funeral practices are characterized by numerous skeletal alterations, especially in the western area of the site where their concentrations are particularly significant. These are not ossuaries but rather supernumerary bones present in the fills of graves of subjects in place or old tombs where no skeletons in place are preserved. These alterations mark the areas where burials are most frequent. The 3D reconstruction is coupled with geostatistical analyses (heatmap and Moran’s index), considering the digging of the land, the concentration of residual artefacts found in the graves, but also the biological characteristics of the sample and the funeral practices uncovered. From 2D entities generated with GIS software, the process of the elevation and sculpture of the volumes is innovative, because even if it is carried out by precise but classical computer graphics techniques, it is led by advanced taphonomical and anthropological reflections. This makes it possible to propose empty spaces, a potential gathering area for the village community and circulation paths. These elements are essential in order to go beyond the storytelling often proposed in archaeology and propose a vision based on the coherence of the observed facts. Even when the archaeological remains are only sunken (no preserved elevation), the integration of multisource archaeological data (biological anthropology, funerary, artefacts and pit size) allows relevant 3D reconstructions as a formidable tool for discussing past occupations. Three-dimensional technologies make it possible to recreate a lost environment to allow a better understanding of the site. They are didactic and help to share data between researchers and/or the public, especially when they are invisible such as the presence of empty space.

Zinc (Zn) isotope ratios of dental enamel are a promising tracer for dietary reconstruction in archeology, but its use is still in its infancy. A recent study demonstrated a high risk of Zn contamination from nitrile, and latex gloves used during chemical sample preparation. Here we assess the potential impact of the use of such gloves during enamel sampling on the Zn isotope composition of teeth from a population of early Holocene hunter gatherers from Lapa do Santo, Lagoa Santa, Minas Gerais, Brazil. We first examined the amount of Zn and its isotopic composition released from the gloves used in this study by soaking them in weak nitric acid and water. We compared Zn isotope ratios obtained from teeth that were sampled wearing nitrile, latex or no gloves. Finally, we performed a linear mixed model (LMM) to investigate post hoc the relationship between the gloves used for sampling and the Zn isotope variability in dental enamel. We found that the gloves used in this study released a similar amount of Zn compared to previous work, but only in acidic solution. Zn isotope ratios of teeth and the LMM identified no sign of significant Zn coming from the gloves when teeth were handled for enamel sampling. We hypothesize that Zn in gloves is mostly released by contact with acids. We found that the main source of Zn isotope variability in the Lapa do Santo population was related to the developmental stage of the tooth tissues sampled. We report identical results for two individuals coming from a different archeological context. Tooth enamel formed in utero and/or during the two first years of life showed higher Zn isotope ratios than enamel formed after weaning. More work is required to systematically investigate if Zn isotopes can be used as a breastfeeding tracer.

Mass graves are usually key historical markers with strong incentive for archeological investigations. The identification of individuals buried in mass graves has long benefitted from traditional historical, archaeological, anthropological and paleopathological techniques. The addition of novel methods including genetic, genomic and isotopic geochemistry have renewed interest in solving unidentified mass graves. In this study, we demonstrate that the combined use of these techniques allows the identification of the individuals found in two Breton historical mass graves, where one method alone would not have revealed the importance of this discovery. The skeletons likely belong to soldiers from the two enemy armies who fought during a major event of Breton history: the siege of Rennes in 1491, which ended by the wedding of the Duchess of Brittany with the King of France and signaled the end of the independence of the region. Our study highlights the value of interdisciplinary approaches with a particular emphasis on increasingly accurate isotopic markers. The development of the sulfur isoscape and testing of the triple isotope geographic assignment are detailed in a companion paper [13].

Here we report Sr and Zn isotope ratios of teeth of medieval to early modern Breton people a population whose diet is known from historical, archeological and collagen isotope data. Most of the population, buried in the Dominican convent of Rennes, France, consists of parliamentary nobles, wealthy commoners and ecclesiastics, who had a diet rich in animal products. Our aim is to assess how the Zn isotope ratios of their teeth compare to those of other French historical populations previously studied, which were characterized by cereal-based diets, and those of modern French individuals, who daily eat animal products. We describe a clear offset (∼0.35‰) between local and non-local human individuals in Zn isotope ratios. The δ 66 Zn tooth values of local individuals overlap that of modern French people, and are lower than those of local carnivores. Non-local δ 66 Zn values are similar to those of historical individuals analyzed previously. We conclude the lower Zn isotope ratios of local humans relative to the associated fauna can be explained by the consumption of carnivorous fish and pork, in agreement with historical, zooarchaeological and collagen (C, N, S) isotope data. Zn isotopes could therefore be a tracer of fish and/or substantial meat consumption in ancient populations.

The evolution of funeral practices from the Middle Ages through the Modern era in Europe is generally seen as a process of secularization. The study, through imaging and autopsy, of two mummies, five lead urns containing hearts, and more than six hundred skeletons of nobles and clergymen from a Renaissance convent in Brittany has led us to reject this view. In addition to exceptional embalming, we observed instances in which hearts alone had been extracted, a phenomenon that had never before been described, and brains alone as well, and instances in which each spouse's heart had been placed on the other's coffin. In some identified cases we were able to establish links between the religious attitudes of given individuals and either ancient Medieval practices or more modern ones generated by the Council of Trent. All of these practices, which were a function of social status, were rooted in religion. They offer no evidence of secularization whatsoever.

Archaeological 3D digitization of skeletal elements is an essential aspect of the discipline. Objectives are various: archiving of data (especially before destructive sampling for biomolecular studies for example), study or for pedagogical purposes to allow their manipulation. As techniques are rapidly evolving, the question that arises is the use of appropriate methods to answer the different questions and guarantee sufficient quality of information. The combined use of different 3D technologies for the study of a single Mesolithic bone fragment from Brittany (France) is here an opportunity to compare different 3D digitization methods. This oldest human bone of Brittany, a clavicle constituted of two pieces, was dug up from the mesolithic shell midden of Beg-er-Vil in Quiberon and dated from ca. 8200 to 8000 years BP. They are bound to post-mortem processing, realized on fresh bone in order to remove the integuments, which it is necessary to better qualify. The clavicle was studied through a process that combines advanced 3D image acquisition, 3D processing, and 3D printing with the goal to provide relevant support for the experts involved in the work. The bones were first studied with a metallographic microscopy, scanned with a CT scan, and digitized with photogrammetry in order to get a high quality textured model. The CT scan appeared to be insufficient for a detailed analysis; the study was thus completed with a µ-CT providing a very accurate 3D model of the bone. Several 3D-printed copies of the collarbone were produced in order to support knowledge sharing between the experts involved in the study. The 3D models generated from µCT and photogrammetry were combined to provide an accurate and detailed 3D model. This model was used to study desquamation and the different cut marks, including their angle of attack. These cut marks were also studied with traditional binoculars and digital microscopy. This last technique allowed characterizing their type, revealing a probable meat cutting process with a flint tool. This work of crossed analyses allows us to document a fundamental patrimonial piece, and to ensure its preservation. Copies are also available for the regional museums.

Diet is a key to evaluating social and health inequalities over time, as it reflects disparities in access to resources often linked to socioeconomic and gender factors. Stable carbon and nitrogen isotopes, while semi-quantitative, typically limit intersite comparisons, as the results are tied to local baseline isotope values. In this study, we overcome this limitation by applying the interdecile ratio—a metric from economics—to isotope data from 12,281 individuals across 393 European sites over millennia. Our isotope-based dietary inequality index reveals the nonlinear evolution of dietary disparities over time and across different geographical areas. Sex-based disparities are evident throughout all time periods. Male individuals are consistently overrepresented in the upper deciles, indicating greater access to animal proteins, while females dominate the lower deciles, reflecting more restricted access. Neolithic societies exhibit homogeneous diets at the population level, but animal protein consumption tends to differ between men and women. As expected, Bronze Age carbon interdecile indexes mark increasing dietary inequality, likely linked to agricultural advances and social hierarchies. Dietary disparities peak during Antiquity, although the gap between the sexes narrows slightly. This diachronic analysis highlights the complex interactions between diet, social structures, and gender and provides a robust framework for comparative studies of health inequalities in archaeology.

Zinc (Zn) isotope ratios of dental enamel are a promising tracer for dietary reconstruction in archeology, but its use is still in its infancy. A recent study demonstrated a high risk of Zn contamination from nitrile, and latex gloves used during chemical sample preparation. Here we assess the potential impact of the use of such gloves during enamel sampling on the Zn isotope composition of teeth from a population of early Holocene hunter gatherers from Lapa do Santo, Lagoa Santa, Minas Gerais, Brazil. We first examined the amount of Zn and its isotopic composition released from the gloves used in this study by soaking them in weak nitric acid and water. We compared Zn isotope ratios obtained from teeth that were sampled wearing nitrile, latex or no gloves. Finally, we performed a linear mixed model (LMM) to investigate post hoc the relationship between the gloves used for sampling and the Zn isotope variability in dental enamel. We found that the gloves used in this study released a similar amount of Zn compared to previous work, but only in acidic solution. Zn isotope ratios of teeth and the LMM identified no sign of significant Zn coming from the gloves when teeth were handled for enamel sampling. We hypothesize that Zn in gloves is mostly released by contact with acids. We found that the main source of Zn isotope variability in the Lapa do Santo population was related to the developmental stage of the tooth tissues sampled. We report identical results for two individuals coming from a different archeological context. Tooth enamel formed in utero and/or during the two first years of life showed higher Zn isotope ratios than enamel formed after weaning. More work is required to systematically investigate if Zn isotopes can be used as a breastfeeding tracer.

Occasionally mentioned in written sources since the Early Middle Ages, embalming with evisceration spreads considerably and becomes quite usual practice for aristocratic elites from the late 13th century to the early 19th century. Apothecaries prepare aromatic powders including many plant organs and exudates supposed to preserve the body. Numerous encyclopaedias and treatises of medicine list these ingredients and show that the recipes are very diverse. Moreover, several studies of embalmed bodies have demonstrated the potential of archaeobotanical analyses coupling pollen and macro-remains for identifying this material. This paper aims to compile a synthesis of written and archaeological sources from France and Italy, and to assess the relevance of an interdisciplinary approach for a better understanding of this aristocratic burial practice. It demonstrates that both types of sources and approaches are strongly complementary and highlights their inherent advantages and methodological limitations. In order to understand which criteria were determinant in the composition of the embalming powders, their medicinal, odoriferous and symbolic properties are discussed. Finally, this work proposes some methodological perspectives and triggers new research avenues on the history of pharmacy in Late Medieval and modern Europe.