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Currently, it is still a tough task for dentists to remineralize dentine in deep caries. The aim of this study was to remineralize demineralized dentine in a tooth model of deep caries using nanocomplexes of carboxymethyl chitosan/amorphous calcium phosphate (CMC/ACP) based on mimicking the stabilizing effect of dentine matrix protein 1 (DMP1) on ACP in the biomineralization of dentine. The experimental results indicate that CMC can stabilize ACP to form nanocomplexes of CMC/ACP, which is able to be processed into scaffolds by lyophilization. In the single-layer collagen model, ACP nanoparticles are released from scaffolds of CMC/ACP nanocomplexes dissolved and then infiltrate into collagen fibrils via the gap zones (40 nm) to accomplish intrafibrillar mineralization of collagen. With this method, the completely demineralized dentine was partially remineralized in the tooth mode. This is a bottom-up remineralizing strategy based on non-classical crystallization theory. Since nanocomplexes of CMC/ACP show a promising effect of remineralization on demineralized dentine via biomimetic strategy, thereby preserving dentinal tissue to the maximum extent possible, it would be a potential indirect pulp capping (IPC) material for the management of deep caries during vital pulp therapy based on the concept of minimally invasive dentistry (MID).

Achieving oriented and ordered remineralization on the surface of demineralized dental enamel, thereby restoring the satisfactory mechanical properties approaching those of sound enamel, is still a challenge for dentists. To mimic the natural biomineralization approach for enamel remineralization, the biological process of enamel development proteins, such as amelogenin, was simulated in this study. In this work, carboxymethyl chitosan (CMC) conjugated with alendronate (ALN) was applied to stabilize amorphous calcium phosphate (ACP) to form CMC/ACP nanoparticles. Sodium hypochlorite (NaClO) functioned as the protease which decompose amelogenin in vivo to degrade the CMC-ALN matrix and generate HAP@ACP core-shell nanoparticles. Finally, when guided by 10 mM glycine (Gly), HAP@ACP nanoparticles can arrange orderly and subsequently transform from an amorphous phase to well-ordered rod-like apatite crystals to achieve oriented and ordered biomimetic remineralization on acid-etched enamel surfaces. This biomimetic remineralization process is achieved through the oriented attachment (OA) of nanoparticles based on non-classical crystallization theory. These results indicate that finding and developing analogues of natural proteins such as amelogenin involved in the biomineralization by natural macromolecular polymers and imitating the process of biomineralization would be an effective strategy for enamel remineralization. Furthermore, this method represents a promising method for the management of early caries in minimal invasive dentistry (MID).

Remineralization of enamel plays a crucial role in the progression of carious process and the management of early caries lesion. Based on the influence of phosphorylated proteins in biomineralization, the objective of this study was to synthesize nano-complexes of phosphorylated chitosan and amorphous calcium phosphate (Pchi–ACP), and evaluate their ability to remineralize enamel subsurface lesions in vitro. Pchi was synthesized using a previously established chemical method. The biomimetic remineralizing solution containing nano-complexes of Pchi–ACP was prepared by adding CaCl 2 and K 2 HPO 4 into Pchi–ACP solution (0.5 % w/v) in sequence. The final concentrations of calcium and phosphate ions were 10 and 6 mM, respectively. The nano-complexes of Pchi–ACP were characterized by Fourier-transform infrared (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM), and selected area electron diffraction (SAED). During testing the enamel lesions were treated with Pchi–ACP and fluoridated remineralizing solutions, respectively. The remineralizing of enamel lesions was examined with field emission electron microscope (FE-SEM) and Micro-CT. ACP was stabilized by Pchi to form nano-complexes that were soluble in water. The size of Pchi–ACP nano-complexes particles was determined to be less than 50 nm. XRD and SAED results confirmed their amorphous phases. FE-SEM and Micro-CT results showed that the remineralizing effect of Pchi–ACP on enamel lesions was similar to that of fluoride. However, the remineralizing rate of Pchi–ACP treatment was significantly higher than that of fluoride treatment ( P  < 0.05). This study highlighted the potential of nanoparticles functionalized with a natural analogue involved in biomineralization, to remineralize early enamel caries.

In order to study the compressive properties of ultra-high performance concrete (UHPC) mixed with coarse aggregate and steel slag powder under different confining pressures, provide technical parameters for the application of UHPC in constrained structures such as steel pipes or stirrups, 20 UHPC specimens were made with 5 different mix proportions. Triaxial compressive tests were carried out under four confining pressures (0 MPa, 5 MPa, 10 MPa and 15 MPa) by using Rock Top full stress multi-field coupling triaxial apparatus, and the stress-strain curves of UHPC specimens were obtained, and the failure of UHPC specimens was analysed. The results show that under uniaxial condition, the UHPC test block of steel slag powder is in the form of longitudinal splitting failure, when the confining pressure is 5 MPa and 10 MPa, the test block is in the form of oblique shear failure, and when the confining pressure is 15 MPa, the test block is in the form of horizontal shear failure of multiple cracks in the middle. The stress-strain curve of UHPC steel slag powder is basically straight in the front section. When there is no confining pressure, the curve has obvious peaks, and the curve drops rapidly after the peak point. When the confining pressure value σ 2  ≥ 5 MPa, with the increase of confining pressure value, the rising section and peak point of the curve increase, and the downward trend after the peak point slows down. The peak stress and strain increase with the increase of confining pressure, but the growth rate slows down slightly and the deformation ability increases. With the increase of crushed stone content, the peak stress and strain of the specimen gradually decrease under the same confining pressure. Adding steel fibre can effectively strengthen the bond between cement matrix and make the specimen have good ductility.

Electrochromic materials were discovered in the 1960s when scientists observed reversible changes between the light and dark states in WO 3 thin films under different voltages. Since then, researchers have identified various electrochromic material systems, including transition metal oxides, polymer materials, and small molecules. However, the electrochromic phenomenon has rarely been observed in non-metallic elemental substances. Herein, we propose the development of non-metallic iodine electrodeposition-based electrochromic dynamic windows using a water-in-salt electrolyte containing iodine ions. The unique electrolyte environment and solvation structure of the water-in-salt electrolyte suppress the dissolution and shuttle effect of iodine, thereby achieving a different reaction pathway compared to traditional electrolytes. This pathway involves a reversible solid-liquid transition between solid iodine and solvated iodide ions. The iodine electrodeposition-based electrochromic dynamic window demonstrates a high optical contrast of 76.0% with near colour neutrality and excellent cycling stability. A practical 400 cm 2 complementary dynamic window is fabricated to demonstrate good electrochromic performance, including high optical contrast, a near colour-neutral opaque state, fast response time, uniform modulation, and polarity-switchable functionality. Several electrochromic material systems have been described but the electrochromic phenomenon has rarely been observed in non-metallic elemental substances. Here, the authors describe iodine electrodeposition-based electrochromic windows using a water-in-salt electrolyte containing iodine ions, achieving optical contrast, near colour neutrality, and excellent cycling stability.

Laterite is the predominant zonal soil in China’s southernmost tropical rainforest and monsoon forest regions, where typhoons are the primary source of precipitation. These storms pose significant risks of land and soil degradation due to heavy rainfall. In recent years, a substantial area of sloping land has been converted to agricultural use in these regions, predominantly for the cultivation of crops grown in laterite soil. These activities contribute to soil erosion, exacerbate environmental challenges, and hinder the pursuit of sustainable development. There is a paucity of research reports on the processes and mechanisms of runoff and sediment on sugarcane-cropped slopes in regions with laterite soil under heavy rainfall conditions. In this study, four different heavy rainfall scenarios of 75, 100, 125, and 150 mm/h were designed to assess the impact on sugarcane growth at four key stages and to measure the resulting effects on initial runoff time, surface runoff, and sediment yield from laterite soil slopes under controlled laboratory conditions. The results showed that the Horton model explained much of the variation in infiltration rate on the sugarcane-cropped laterite slopes. The cumulative sediment yield on the sugarcane-cropped laterite slopes followed a second-degree polynomial function. The initial runoff time, infiltration intensity, runoff intensity, and sediment yield were all linearly related to the leaf area index (LAI) and rainfall intensity on the sugarcane-cropped slope surface. The leaf area index exerted a greater influence on the initial runoff time and infiltration intensity than rainfall intensity. However, rainfall intensity exerted a greater influence on the runoff intensity and sediment yield than the leaf area index. Compared with the bare sloping land, the average sediment yield was reduced by 12.2, 33.1, 58.2, and 64.9% with the sugarcane growth stages of seedling, tillering, elongation, and maturity, respectively.

RationaleNeuroinflammation may inhibit oligodendrocyte and astrocyte differentiation, which causes demyelination and synaptic degeneration. The myelin component nervonic acid (NA) may improve demyelinating and neurodegenerative diseases.ObjectivesThis study firstly explored relationships between glial cell dysfunction and demyelination or synaptic degeneration in schizophrenia patients, and secondly determined nervonic acid therapeutic effects in a preclinical schizophrenia model of mice.MethodsPlasma samples were collected from 18 male healthy controls and 18 male schizophrenic patients (diagnosed by DSM-V) at aged 18–55. Mouse brain samples were collected from a maternal immune activation (MIA) model of schizophrenia via injecting 5 mg/kg polyinosinic-polycytidylic acid. Male mouse offspring (age 2.5 months, n = 12) were treated by clozapine (15 mg/kg/day) or fed 0.5% NA for 6 weeks. Cytokine and dopamine (DA) concentrations, and glial phenotypes and myelin markers were measured in both human plasma and mouse brain samples.ResultsIn patient plasma, increased proinflammatory cytokines were associated with reactive microglia (Iba-1) up-regulation, while decreased anti-inflammatory cytokines were related to microglia (CD206) downregulation. Decreased astrocyte marker (p11) concentrations were accompanied by reduced concentrations of oligodendrocyte and synaptic markers. However, NA and DA contents were increased. Compared with control mice, SZ-like behaviors appeared in MIA male mice. Changes in microglia and astrocytes markers, and cytokine concentrations in the frontal cortex were consistent with those observed in patients’ plasma. Hippocampal oligodendrocyte and synaptic marker expression were also decreased. DA content and DA/metabolite (DAPOC) were increased in MIA mouse brains. Most of these changes were normalized by both clozapine and NA. Even though some NA effects were more pronounced than clozapine, only clozapine restored cytokine function.ConclusionThe data suggest a possible therapeutic route for schizophrenia patients.

Non-communicable diseases have replaced infectious diseases as the leading cause of death among urban residents; the percentage of years of life lost because of such diseases as a fraction of all-cause years of life lost increased from 50·0% (95% CI 48·5–53·0) in 1990 to 77·3% (76·5–78·1) in 2015.4 Health inequality also increased in urban areas.5 China has acted to address urban health challenges by passing strict environmental regulations and investing heavily in urban infrastructure.In traditional chinese medicine, human health is seen as the consequence of harmonisation between human beings and their environments and between the various parts of the human body and the focus is on disease prevention rather than treatment.16 Concepts from traditional Chinese medicine, such as the maintenance of regular daily activities (rest, diet, and exercise) and avoidance of negative health effects from environmental factors (eg, Fengshui) have become essential parts of Chinese culture.In 2007 alone, particulate matter with a diameter of less than 2·5 μm (PM2·5) affected the productivity of about 72 million workers in 30 Chinese provinces, causing an estimated economic loss of ¥346·3 billion (US$44·4 billion, about 1·1% of the national gross domestic product [GDP]).19 Total economic losses attributed to the public health effects of pollution from particulate matter with a diameter of less than 10 μm (PM10) and sulphur dioxide (SO2) pollution in 74 cities were estimated to be as high as ¥439·8 billion ($70·9 billion, about 2·3% of these cities' GDP) in the first half of 2015.20 China will also have a massive future burden of non-communicable diseases—most of which will affect urban areas—that will strain future health systems and limit economic growth in the country.Total annual premature deaths from such diseases are expected to increase from 3·11 million in 2013 to 3·52 million in 2030.21 Between 2012 and 2030, economic losses attributable to five key non-communicable diseases–ischaemic heart disease, cerebrovascular disease, diabetes, breast cancer, and chronic obstructive pulmonary disease–will total $23·03 trillion USD,22 which is more than twice of China's total GDP in 2015 ($11·07 trillion).

Chronic stress is the primary environmental risk factor for major depressive disorder (MDD), and there is compelling evidence that neuroinflammation is the major pathomechanism linking chronic stress to MDD. Mitogen-activated protein kinase (MAPK) phosphatase-1 (MKP-1) is a negative regulator of MAPK signaling pathways involved in cellular stress responses, survival, and neuroinflammation. We examined the possible contributions of MKP-1 to stress-induced MDD by comparing depression-like behaviors (anhedonia, motor retardation, behavioral despair), neuroinflammatory marker expression, and MAPK signaling pathways among rats exposed to chronic unpredictable mild stress (CUMS), overexpressing MKP-1 in the hippocampus, and CUMS-exposed rats underexpressing MKP-1 in the hippocampus. Rats exposed to CUMS exhibited MKP-1 overexpression, greater numbers of activated microglia, and enhanced expressions of neuroinflammatory markers (interleukin [IL]-6, [IL]-1β, tumor necrosis factor [TNF]-ɑ, and decreased phosphorylation levels of ERK and p38 in the hippocampus as well as anhedonia in the sucrose preference test, motor retardation in the open field, and greater immobility (despair) in the forced swimming tests. These signs of neuroinflammation and depression-like behaviors and phosphorylation levels of ERK and p38 were also observed in rats overexpressing MKP-1 without CUMS exposure, while CUMS-induced neuroinflammation, microglial activation, phosphorylation levels of ERK and p38, and depression-like behaviors were significantly reversed by MKP-1 knockdown. Moreover, MKP-1 knockdown promoted the activation of the MAPK isoform ERK, implying that the antidepressant-like effects of MKP-1 knockdown may be mediated by the ERK pathway disinhibition. These findings suggested that hippocampal MKP-1 is an essential regulator of stress-induced neuroinflammation and a promising target for antidepressant development.

BackgroundDifferential diagnosis of benign and malignant thyroid imaging reporting and data system category 4 (TI-RADS-4) nodules can be difficult using conventional ultrasound (US). This study aimed to evaluate whether multimodal ultrasound imaging can improve differentiation and characterization of benign and malignant TI-RADS-4 nodules.MethodsMultimodal ultrasound imaging, including US, superb microvascular imaging (SMI), and real-time elastography (RTE), were performed on 196 TI-RADS-4 nodules (78, benign; 118, malignant) in 170 consecutive patients. The sensitivity, specificity, accuracy, false negative rate (FNR), and false positive rate (FPR) of each single method and that of multimodal US imaging were determined by comparison with surgical pathology results.ResultsThe sensitivity, specificity, accuracy, FNR, and FPR for US were 65.25%, 69.23%, 66.84%, 34.75%, 30.77%, respectively; for SMI were 77.97%, 93.59%, 84.18%, 22.03%, 6.41%, respectively; RTE, 80.51%, 84.62%, 82.14%, 19.49%, 15.38%; and for multimodal US imaging were 94.08%, 87.18%, 91.33%, 6.93%, 12.82%, respectively. The areas under the received operating characteristic curve for US, SMI, RTE, and multimodal US imaging in evaluating benign and malignant TI-RADS-4 nodules were 67.2%, 84.40%, 86.60%, and 95.50%, respectively.ConclusionsThe initial clinical results suggest that multimodal US imaging improves the diagnostic accuracy of TI-RADS-4 nodules and provides additional information for differentiating malignant and benign nodules.