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An exploration of tourist locales that have been restored or adapted to preserve some aspect of the history of the American South.

Mitigating loss of genetic diversity is a major global biodiversity challenge 1 , 2 , 3 – 4 . To meet recent international commitments to maintain genetic diversity within species 5 , 6 , we need to understand relationships between threats, conservation management and genetic diversity change. Here we conduct a global analysis of genetic diversity change via meta-analysis of all available temporal measures of genetic diversity from more than three decades of research. We show that within-population genetic diversity is being lost over timescales likely to have been impacted by human activities, and that some conservation actions may mitigate this loss. Our dataset includes 628 species (animals, plants, fungi and chromists) across all terrestrial and most marine realms on Earth. Threats impacted two-thirds of the populations that we analysed, and less than half of the populations analysed received conservation management. Genetic diversity loss occurs globally and is a realistic prediction for many species, especially birds and mammals, in the face of threats such as land use change, disease, abiotic natural phenomena and harvesting or harassment. Conservation strategies designed to improve environmental conditions, increase population growth rates and introduce new individuals (for example, restoring connectivity or performing translocations) may maintain or even increase genetic diversity. Our findings underscore the urgent need for active, genetically informed conservation interventions to halt genetic diversity loss. A comprehensive meta-analysis of global terrestrial and marine genetic diversity covering more than three decades of research demonstrates rapid loss of genetic diversity and identifies conservation interventions that could mitigate this process.

Accurately estimating effective population size (Ne) is essential for understanding evolutionary processes and guiding conservation efforts. This study investigates Ne estimation methods in spatially structured populations using a population of moor frog (Rana arvalis) as a case study. We assessed the behaviour of Ne estimates derived from the linkage disequilibrium (LD) method as we changed the spatial configuration of samples. Moor frog eggs were sampled from 25 breeding patches (i.e., separate vernal ponds, ditches or parts of larger fens) within a single population, revealing an isolation‐by‐distance pattern and a local spatial genetic structure. Varying buffer sizes around each patch were used to examine the impact of sampling window size on the estimation of effective number of breeders (Nb). Our results indicate a downward bias in LD Nb estimates with increasing buffer size, suggesting an underestimation of Nb. The observed bias is attributed to LD resulting from including genetically divergent individuals (mixture‐LD) confounding LD due to drift. This emphasises the significance of considering even subtle spatial genetic patterns. The implications of these findings are discussed, emphasising the need to account for spatial genetic structure to accurately assess population viability and inform conservation efforts. This study contributes to our understanding of the challenges associated with Ne estimation in spatially structured populations and underscores the importance of refining methodologies to address population‐specific spatial dynamics for effective conservation planning and management.

Within the HIV clinical trial field, there are gaps in existing ethical regulations in relation to post-trial care. There is need to develop post-trial care guidelines that are flexible and sensitive to local contexts and to the specific needs of different groups of participants, particularly in low income contexts. Evidence regarding HIV trial closure and post-trial care is required to underpin the development of appropriate policies in this area. This article reports research from Uganda that develops a new model of ‘Facilitated Transition’ to conceptualize the transition process of HIV positive trial participants from ‘research’ to ‘usual care’ health facilities after trial conclusion. This was a qualitative grounded theory study that included 21 adult HIV positive post-trial participants and 22 research staff, undertaken between October 2014 and August 2015. The findings showed that trial closure is a complex process for HIV positive participants which includes three phases: the pre-closure, trial-closure, and post-trial phases. The model highlights a range of different needs of research participants and suggests specific and person-centred interventions that can be delivered at different phases with the aim of improving health outcomes and experiences for trial participants in low income settings during trial closure. Further research needs to be done to verify the model in other contexts and for other conditions.

Once upon a time, it was impossible to drive through the South without coming across signs to \"See Rock City\" or similar tourist attractions. From battlegrounds to birthplaces, and sites in between, heritage tourism has always been part of how the South attracts visitors--and defines itself--yet such sites are often understudied in the scholarly literature. As the contributors to this volume make clear, the narrative of southern history told at these sites is often complicated by race, influenced by local politics, and shaped by competing memories. Included are essays on the meanings of New Orleans cemeteries; Stone Mountain, Georgia; historic Charleston, South Carolina; Yorktown National Battlefield; Selma, Alabama, as locus of the civil rights movement; and the homes of Mark Twain, Margaret Mitchell, and other notables. Destination Dixie reveals that heritage tourism in the South is about more than just marketing destinations and filling hotel rooms; it cuts to the heart of how southerners seek to shape their identity and image for a broader touring public--now often made up of northerners and southerners alike.

Background Facilitation is a promising implementation intervention, which requires theory-informed evaluation. This paper presents an exemplar of a multi-country realist process evaluation that was embedded in the first international randomised controlled trial evaluating two types of facilitation for implementing urinary continence care recommendations. We aimed to uncover what worked (and did not work), for whom, how, why and in what circumstances during the process of implementing the facilitation interventions in practice. Methods This realist process evaluation included theory formulation, theory testing and refining. Data were collected in 24 care home sites across four European countries. Data were collected over four time points using multiple qualitative methods: observation (372 h), interviews with staff ( n  = 357), residents ( n  = 152), next of kin ( n  = 109) and other stakeholders ( n  = 128), supplemented by facilitator activity logs. A combined inductive and deductive data analysis process focused on realist theory refinement and testing. Results The content and approach of the two facilitation programmes prompted variable opportunities to align and realign support with the needs and expectations of facilitators and homes. This influenced their level of confidence in fulfilling the facilitator role and ability to deliver the intervention as planned. The success of intervention implementation was largely dependent on whether sites prioritised their involvement in both the study and the facilitation programme. In contexts where the study was prioritised (including release of resources) and where managers and staff support was sustained, this prompted collective engagement (as an attitude and action). Internal facilitators’ (IF) personal characteristics and abilities, including personal and formal authority, in combination with a supportive environment prompted by managers triggered the potential for learning over time. Learning over time resulted in a sense of confidence and personal growth, and enactment of the facilitation role, which resulted in practice changes. Conclusion The scale and multi-country nature of this study provided a novel context to conduct one of the few trial embedded realist-informed process evaluations. In addition to providing an explanatory account of implementation processes, a conceptual platform for future facilitation research is presented. Finally, a realist-informed process evaluation framework is outlined, which could inform future research of this nature. Trial registration Current controlled trials ISRCTN11598502 .

Background This study explores with patients, carers and health care professionals if, when and how Advance Care Planning conversations about patients’ preferences for place of care (and death) were facilitated and documented. Methods The study adopted an exploratory case study design using qualitative interviews, across five services delivering palliative care to cancer and non-cancer patients within an urban and rural English region. The study recruited 18 cases made up of patients (N = 18; 10 men; 8 women; median age 75); nominated relatives (N = 11; 7 women; 4 men; median age 65) and healthcare professionals (N = 15) caring for the patient. Data collection included: 18 initial interviews (nine separate interviews with patients and 9 joint interviews with patients and relatives) and follow up interviews in 6 cases (involving a total of 5 patients and 5 relatives) within one year of the first interview. Five group interviews were conducted with 15 healthcare professionals; 8 of whom also participated in follow up interviews to review their involvement with patients in our study. Results Patients demonstrated varying degrees of reticence, evasion or reluctance to initiate any conversations about end of life care preferences. Most assumed that staff would initiate such conversations, while staff were often hesitant to do so. Staff-identified barriers included the perceived risks of taking away hope and issues of timing. Staff were often guided by cues from the patient or by intuition about when to initiate these discussions. Conclusions This study provides insights into the complexities surrounding the initiation of Advance Care Planning involving conversations about end of life care preferences with patients who are identified as having palliative care needs, in particular in relation to the risks inherent in the process of having conversations where mortality must be acknowledged. Future research is needed to examine how to develop interventions to help initiate conversations to develop person centred plans to manage the end of life.

Adult bones have a notable regenerative capacity. Over 40 years ago, an intrinsic activity capable of initiating this reparative response was found to reside within bone itself, and the term bone morphogenetic protein 1 (BMP) was coined to describe the molecules responsible for it. A family of BMP proteins was subsequently identified 2 , 3 , 4 , but no individual BMP has been shown to be the initiator of the endogenous bone repair response. Here we demonstrate that BMP2 is a necessary component of the signaling cascade that governs fracture repair. Mice lacking the ability to produce BMP2 in their limb bones have spontaneous fractures that do not resolve with time. In fact, in bones lacking BMP2, the earliest steps of fracture healing seem to be blocked. Although other osteogenic stimuli are still present in the limb skeleton of BMP2-deficient mice, they cannot compensate for the absence of BMP2. Collectively, our results identify BMP2 as an endogenous mediator necessary for fracture repair.

Background Despite the increasing number of epigenomic studies in plants, little is known about the forces that shape the methylome in long-lived woody perennials. The Lombardy poplar offers an ideal opportunity to investigate the impact of the individual environmental history of trees on the methylome. Results We present the results of three interconnected experiments on Lombardy poplar. In the first experiment, we investigated methylome variability during a growing season and across vegetatively reproduced generations. We found that ramets collected over Europe and raised in common conditions have stable methylomes in symmetrical CG-contexts. In contrast, seasonal dynamics occurred in methylation patterns in CHH context. In the second experiment, we investigated whether methylome patterns of plants grown in a non-parental environment correlate with the parental climate. We did not observe a biological relevant pattern that significantly correlates with the parental climate. Finally, we investigated whether the parental environment has persistent carry-over effects on the vegetative offspring’s phenotype. We combined new bud set observations of three consecutive growing seasons with former published bud set data. Using a linear mixed effects analysis, we found a statistically significant but weak short-term, parental carry-over effect on the timing of bud set. However, this effect was negligible compared to the direct effects of the offspring environment. Conclusions Genome-wide cytosine methylation patterns in symmetrical CG-context are stable in Lombardy poplar and appear to be mainly the result of random processes. In this widespread poplar clone, methylation patterns in CG-context can be used as biomarkers to infer a common ancestor and thus to investigate the recent environmental history of a specific Lombardy poplar. The Lombardy poplar shows high phenotypic plasticity in a novel environment which enabled this clonal tree to adapt and survive all over the temperate regions of the world.

Medium‐sized carnivores, including the synanthropic stone marten (Martes foina Erxleben, 1777), have shown remarkable adaptability to urbanised and fragmented landscapes, facilitating their spread across mainland Europe. This study investigates the recolonisation of a highly urbanised region by stone martens within two decades, examining spatial and temporal genome‐wide data (using genotyping by sequencing) to reveal colonisation dynamics, sources, and barriers influencing their expansion. Using genotypes from 5536 SNPs across 376 stone martens collected between 1995 and 2013, our findings indicate that stone martens successfully expanded through urban environments, yet dispersal was neither entirely random nor strictly distance‐dependent. The initial southeastern stronghold (E1) showed the lowest genetic diversity and limited spatial expansion, while other population sources contributed to recolonisation, highlighting a complex, multi‐source expansion. Gene flow in the early stages was largely confined to E1, progressing northward and eventually enabling exchange with a second eastern lineage (E2). Meanwhile, the western lineage displayed higher connectivity, occasionally crossing barriers like motorways. Motorways, however, significantly shaped recolonisation patterns, reducing gene flow, while other elements such as built‐up areas, secondary roads or waterways showed an additional though very small effect. Over the study period, genetic patch size increased, indicating longer dispersal distances. Gene flow strengthened within both eastern (E1 and E2) and western populations. Still, the western population diverged into two subclusters (W1 and W2) of which one became more differentiated. This suggests limited genetic homogenisation in the near future. This study provides insights into the genetic and ecological dynamics of carnivore recolonisation in highly fragmented landscapes. This study investigates the recolonisation of a highly urbanised region by stone martens within two decades, examining spatial and temporal genome‐wide data (using genotyping by sequencing) to reveal colonisation dynamics, sources, and barriers influencing their expansion. Our findings indicate that stone martens successfully expanded through urban environments, yet dispersal was not entirely random nor strictly distance‐dependent. This study underscores the adaptive responses of stone martens to urbanisation, providing insights into the genetic and ecological dynamics of carnivore recolonisation in fragmented landscapes.