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Hepatocellular carcinoma (HCC) is a major leading cause of cancer-related death worldwide. Alpha fetoprotein (AFP) is reactivated in a majority of hepatocellular carcinoma (HCC) and associated with poor patient outcomes. Although increasing evidence has shown that AFP can regulate HCC cell growth, the precise functions of AFP in hepatocarcinogenesis and the associated underlying mechanism remain incompletely understood. In this study, we demostrated that depleting AFP significantly suppressed diethylnitrosamine (DEN)-induced liver tumor progression in an AFP gene-deficient mouse model. Similarly, knocking down AFP expression inhibited human HCC cell proliferation and tumor growth by inducing apoptosis. AFP expression level was inversely associated with the apoptotic rate in mouse and human HCC specimens. Investigation of potential cross-talk between AFP and apoptotic signaling revealed that AFP exerted its growth-promoting effect by suppressing the Fas/FADD-mediated extrinsic apoptotic pathway. Mechanistically, AFP bound to the RNA-binding protein HuR, increasing the accumulation of HuR in the cytoplasm and subsequent inhibition of Fas mRNA translation. In addition, we found that inhibiting AFP enhanced the cytotoxicity of therapeutics to AFP-positive HCC cells by activating HuR-mediated Fas/FADD apoptotic signaling. Conclusion: Our study defined the pro-oncogenic role of AFP in HCC progression and uncovered a novel antiapoptotic mechanism connecting AFP to HuR-mediated Fas translation. Our findings suggest that AFP is involved in the pathogenesis and chemosensitivity of HCC and that blockade of AFP may be a promising strategy to treat advanced HCC.

This study employed the molecular dynamics simulation method to systematically investigate the dynamic response behavior of the molten titanium liquid-vapor interface under high-frequency (50 GHz) and large-amplitude (5%) transverse mechanical cyclic impact. Based on the theory of driven-damped oscillators, we analyzed the steady-state forced oscillation characteristics of dynamic surface tension. Through frequency analysis, the dependence of the system response on the impact frequency was revealed. And by using the liquid stratification method, we investigated the space-time correlation characteristics of the bulk and surface atomic dynamics. This study mainly found that the average value of dynamic surface tension increased by 7% compared to the equilibrium state, confirming that high-frequency mechanical impact has a regulatory effect on surface tension. Meanwhile, the peak and valley of instantaneous fluctuations reached 14% and 5% of the equilibrium state respectively, presenting a significant nonlinear oscillation characteristic. Theoretical analysis indicates that there is a coupling effect between the generalized natural frequency and the damping constant. Experimental observations show that the atomic dynamics behavior of the outermost layer is significantly different from that of the bulk liquid. This study has deepened our understanding of the dynamics of the liquid-vapor interface under extreme conditions. It provides new theoretical basis for understanding the multi-scale behavior of liquid metals and has guiding significance for the surface control of high-frequency mechanical impacts and industrial applications.

This study employed the molecular dynamics simulation method to systematically investigate the dynamic response behavior of the molten titanium liquid-vapor interface under high-frequency (50 GHz) and large-amplitude (5%) transverse mechanical cyclic impact. Based on the theory of driven-damped oscillators, we analyzed the steady-state forced oscillation characteristics of dynamic surface tension. Through frequency analysis, the dependence of the system response on the impact frequency was revealed. And by using the liquid stratification method, we investigated the space-time correlation characteristics of the bulk and surface atomic dynamics. This study mainly found that the average value of dynamic surface tension increased by 7% compared to the equilibrium state, confirming that high-frequency mechanical impact has a regulatory effect on surface tension. Meanwhile, the peak and valley of instantaneous fluctuations reached 14% and 5% of the equilibrium state respectively, presenting a significant nonlinear oscillation characteristic. Theoretical analysis indicates that there is a coupling effect between the generalized natural frequency and the damping constant. Experimental observations show that the atomic dynamics behavior of the outermost layer is significantly different from that of the bulk liquid. This study has deepened our understanding of the dynamics of the liquid-vapor interface under extreme conditions. It provides new theoretical basis for understanding the multi-scale behavior of liquid metals and has guiding significance for the surface control of high-frequency mechanical impacts and industrial applications.

The hypothetical discussion describes the design of a personal computer innovation-based display structure tailored specifically for subordinates enrolled in an educational well-being course. Using a sophisticated approach, this structure intends to raise understudy devotion, urge dynamic studying, and enhance profitability. The displaying framework promotes reflective thinking, resolute thinking, and the acquisition of practical qualities associated with psychological wellness through the use of intuitive multimedia, competitive elements, and tailored input. This innovative displaying structure allows students to grow a more substantial grasp of mental health concerns while growing their personal psychic flourishing in a consistent and enticing teaching environment through the integration of cutting-edge technology with informative content. Modern instructional tactics stay frequently grounded in scheduled exam-based schooling besides infrequently actually caring about the root cause of pupils' questions, although flipping psychological training methods have emerged in some sectors. The primary goal of this concept is to combine digital intelligence with flipping laboratory brain studies. This essay focuses on the characteristics of fraudulent learning as well as the symbolic significance of flipping their house. What exactly are the advantages of the smart display stage over traditional learning? The following piece chooses crucial 1, the classroom, important 2, the school, main 3, the group, and key 4, from our institution's undergraduates. The Al and Bl groups guide an academic year of artificial intellect, along with the concept of inverted classrooms for neuroscience. Bundles A2 and B2 depict the under study in accordance with normal training. According to the study data, the size of gatherings Al and Bl increased respectively. Gatherings Al and Bl had higher midterm and final normal scores respectively, than assemblages A2 and B2, and their scores have increased by a rate. The influence of artificial intelligence and flipping residence mind study on mental health in education programs may additionally energize subordinates' benefit and forward the advancement of understudy's display throughout autonomous education.

Background Most studies have showed that maternal depression is associated with pregnancy complications. However, there were limited evidences in Chinese population. We examined the associations of antenatal depression symptoms with pregnancy outcomes, especially for low birth weight. Methods A total of 1377 singleton pregnant women were recruited from Nanshan Maternity & Child Healthcare Hospital of Shenzhen in this prospective cohort study. Depression symptoms were assessed by the Edinburgh postnatal depression scale (EPDS) questionnaire in the second trimester of gestation; cut-points for the indication of antenatal depression were ≧12 scores in this study. Socio-demographic data, life-style and pregnancy outcomes were collected through Shenzhen Maternity & Child Healthcare database. The risks of adverse outcomes in pregnant women with antenatal depression were determined by multivariate logistic regression and represented as odds ratio(OR) and 95% confidence interval (CI). Results Of the 1377 subjects, the prevalence of antenatal depression was 19.1%. The EPDS scores were 13.8 ± 2.0 and 6.5 ± 2.9 ( P  < 0.001) in subjects with and without antenatal depression, respectively. After adjustment for maternal age, education, parity, pre-pregnancy body mass index (BMI), residential area, fetal gender, an EPDS score ≥ 12 (versus. < 12) was associated with an increased risk for low birth weight (odds ratio: 2.05, 95% CI: 1.12–4.64), but not for preterm birth, large for gestational age, small for gestational age or macrosomia. Conclusion Pregnant women presenting antenatal depressive symptoms are at elevated risk of low birth weight. Mental health problems of pregnancy should be addressed for the prevention of low birth weight.

There are currently no studies exploring omicron infection and male sexual function and semen quality. Our aim was to estimate changes in sexual function and semen quality in men recovering from infection since the COVID-19 Omicron pandemic started in China in late 2022. We collected 1540 questionnaires and assessed male function before infection and acute phase after infection by using International Index of Erectile Function-5, Premature Ejaculation Diagnostic Tool, and Arizona Sexual Experience Scale. We also collected the before and after semen analysis results of 247 male patients. During the acute phase after infection, the proportion of erectile dysfunction was significantly higher than before infection, but ejaculatory function was not significantly altered; In addition, semen analysis showed significant difference in semen concentration, semen activity and PR a+b sperm forward movement after infection compared to pre-infection.: COVID-19 Omicron can affect erectile function as well as sexual experience in male patients in the acute phase. Decreased sexual function due to COVID-19 Omicron may be related to body temperature and anxiety during infection.

There are currently no studies exploring omicron infection and male sexual function and semen quality. Our aim was to estimate changes in sexual function and semen quality in men recovering from infection since the COVID-19 Omicron pandemic started in China in late 2022. We collected 1540 questionnaires and assessed male function before infection and acute phase after infection by using International Index of Erectile Function-5, Premature Ejaculation Diagnostic Tool, and Arizona Sexual Experience Scale. We also collected the before and after semen analysis results of 247 male patients. During the acute phase after infection, the proportion of erectile dysfunction was significantly higher than before infection, but ejaculatory function was not significantly altered; In addition, semen analysis showed significant difference in semen concentration, semen activity and PR a+b sperm forward movement after infection compared to pre-infection.: COVID-19 Omicron can affect erectile function as well as sexual experience in male patients in the acute phase. Decreased sexual function due to COVID-19 Omicron may be related to body temperature and anxiety during infection.

Animal pose estimation is very useful in analyzing animal behavior, monitoring animal health and moving trajectories, etc. However, occlusions, complex backgrounds, and unconstrained illumination conditions in wild-animal images often lead to large errors in pose estimation, i.e., the detected key points have large deviations from their true positions in 2D images. In this paper, we propose a method to improve animal pose estimation accuracy by exploiting 3D prior constraints. Firstly, we learn the 3D animal pose dictionary, in which each atom provides prior knowledge about 3D animal poses. Secondly, given the initially estimated 2D animal pose in the image, we represent its latent 3D pose with the learned dictionary. Finally, the representation coefficients are optimized to minimize the difference between the initially estimated 2D pose and the 2D projection of the latent 3D pose. Furthermore, we construct 2D and 3D animal pose datasets, which are used to evaluate the algorithm’s performance and learn the 3D pose dictionary, respectively. Our experimental results demonstrate that the proposed method makes good use of the 3D pose knowledge and can effectively improve 2D animal pose estimation.

Conversion of sputum from positive to negative is one of the indicators to evaluate the efficacy of anti-tuberculosis treatment (ATT). We investigate the factors associated with delayed sputum conversion after 2 or 5 months of ATT from the perspectives of bacteriology and genomics. A retrospective study of sputum conversion in sputum positive 1782 pulmonary tuberculosis (PTB) was conducted from 2021 to 2022 in Beijing, China. We also designed a case-matched study including 24 pairs of delayed-sputum-conversion patients (DSCPs) and timely-sputum-conversion patients (TSCPs), and collect clinical isolates from DSCPs before and after ATT and initial isolates of TSCPs who successfully achieved sputum conversion to negative after 2 months of ATT. A total of 75 strains were conducted drug sensitivity testing (DST) of 13 anti-TB drugs and whole-genome sequencing (WGS) to analyze the risk factors of delayed conversion and the dynamics changes of drug resistance and genomics of Mycobacterium tuberculosis (MTB) during ATT. We found TSCPs have better treatment outcomes and whose initial isolates show lower levels of drug resistance. Clinical isolates of DSCPs showed dynamically changing of resistance phenotypes and intra-host heterogeneity. Single nucleotide polymorphism (SNP) profiles showed large differences between groups. The study provided insight into the bacteriological and genomic variation of delayed sputum conversion. It would be helpful for early indication of sputum conversion and guidance on ATT.

Background China is a high‐burden country for multidrug‐resistant tuberculosis/rifampin‐resistant tuberculosis (MDR/RR‐TB). Fluoroquinolones (FQs) are key drugs for the treatment of patients with MDR/RR‐TB. However, research on the resistance of FQs in Beijing is limited. Methods We collected clinical isolates from all patients with pulmonary TB in Beijing from January 2016 to December 2021, conducted drug‐sensitivity tests and sequencing for levofloxacin (LFX) and moxifloxacin (MFX), and collected the treatment plans and outcomes of the patients. Results A total of 8512 clinical isolates were collected from patients with pulmonary TB, and 261 RR‐TB strains were screened. The proportions of drug‐sensitive and drug‐resistant strains significantly differed by age group and treatment history. The rates of LFX and MFX resistance were 27.6% (72/261) and 36.4% (95/261), respectively. The detection rates of MDR‐TB and pre‐extensively drug‐resistant TB (pre‐XDR‐TB) were 73.2% (191/261) and 36.4% (95/261), respectively, and the trends were significant (χ2 trend = 9.995, p = 0.002; χ2 trend = 12.744, p = 0.026). Among the 261 RR‐TB strains, 14.9% (24/261) were sensitive to LFX but resistant to MFX. Among the four patients with LFX‐resistant TB who received LFX treatment failed in three patients(Fisher's exact test, p = 0.009). The common mutation sites were 94 and 90 in gyrA. A novel mutation Ala90Ser was discovered. Conclusions FQs resistance trends in RR‐TB patients in Beijing are striking. Strains showed incomplete cross‐resistance to LFX and MFX. Testing for FQs resistance and developing a reasonable treatment plan are recommended. Attention should be given to the changing trends in MDR‐TB and pre‐XDR‐TB. We collected the clinical isolates from all patients with pulmonary TB in Beijing from January 2016 to December 2021, A total of 8,512 clinical isolates were collected, and 261 RR‐TB strains were screened. We conducted the drug sensitivity tests and sequencing for LFX and MFX. The MICs of the strains were mainly distributed at 0.25 μg/mL for both drugs, and among the 261 strains of RR‐TB, 27.2% were resistant to both LFX and MFX, 0.4% were resistant to LFX but sensitive to MFX, 9.2% were sensitive to LFX but resistant to MFX, and 63.2% were sensitive to both LFX and MFX. The MDR‐TB detection rate over this period significantly declined after rebounding in 2019 and 2020, and the pre‐XDR‐TB detection rate slowly decreased from 2016 to 2018 and then continued to decline after a significant increase in 2019. By 2021, the rate had recovered to the 2018 level. After sequencing, we found that the common mutation sites were Asp94, Ala90, Asp91, and Asp88.