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With the increase in iron/steel production, the higher volume of by-products (slag) generated necessitates its efficient recycling. Because the Linz-Donawitz (LD) slag is rich in silicon (Si) and other fertilizer components, we aim to evaluate the impact of the LD slag amendment on soil quality (by measuring soil physicochemical and biological properties), plant nutrient uptake, and strengthens correlations between nutrient uptake and soil bacterial communities. We used 16 S rRNA illumine sequencing to study soil bacterial community and APIZYM assay to study soil enzymes involved in C, N, and P cycling. The LD slag was applied at 2 Mg ha −1 to Japonica and Indica rice cultivated under flooded conditions. The LD slag amendment significantly improved soil pH, plant photosynthesis, soil nutrient availability, and the crop yield, irrespective of cultivars. It significantly increased N, P, and Si uptake of rice straw. The slag amendment enhanced soil microbial biomass, soil enzyme activities and enriched certain bacterial taxa featuring copiotrophic lifestyles and having the potential role for ecosystem services provided to the benefit of the plant. The study evidenced that the short-term LD slag amendment in rice cropping systems is useful to improve soil physicochemical and biological status, and the crop yield.

Inflammatory Breast Cancer (IBC) is a highly aggressive malignancy with distinct clinical and histopathological features whose molecular basis is unresolved. Here we describe a human IBC cell line, A3250, that recapitulates key IBC features in a mouse xenograft model, including skin erythema, diffuse tumor growth, dermal lymphatic invasion, and extensive metastases. A3250 cells express very high levels of the CCL2 chemokine and induce tumors enriched in macrophages. CCL2 knockdown leads to a striking reduction in macrophage densities, tumor proliferation, skin erythema, and metastasis. These results establish IBC-derived CCL2 as a key factor driving macrophage expansion, and indirectly tumor growth, with transcriptomic analysis demonstrating the activation of multiple inflammatory pathways. Finally, primary human IBCs exhibit macrophage infiltration and an enriched macrophage RNA signature. Thus, this human IBC model provides insight into the distinctive biology of IBC, and highlights potential therapeutic approaches to this deadly disease. Inflammatory breast cancer (IBC) is an aggressive form of breast cancer with a poor prognosis. Here the authors report the characterization of a human IBC cell line recapitulating the clinical and histopathological features of the human disease, and implicating its high level of CCL2 in macrophage infiltration and tumor progression.

Climate-smart rice (CSR) is the need of the hour to sustainably increase rice production, but most CSR practices and technologies have not yet taken hold. Here we have outlined the main barriers to CSR adoption, explained the key dimensions, and prioritized suitable CSR production technologies. A viable strategy for CSR production is realized through integrated agronomic management, co-cropping, and breeding rice for climate resilience, high yields, and low methane emissions.

The importance of assessing the impacts of soil arsenic (As) contamination on microbial properties lay on the fact that microbes are instrumental in nutrient cycling and are therefore indicators of soil quality. In this study, soil chemical extraction methods were used to extract labile and freely exchangeable As (water-soluble As and sodium bicarbonate-extractable As), amorphous/crystalline Fe and Mn oxide-bound As (acid ammonium oxalate-extractable As and hydroxylamine hydrochloride-extractable As), and their impacts on microbial biomass (microbial biomass C, total bacterial and fungal biomass, active bacterial and fungal biomass), enzyme activities representing four major soil biogeochemical cycles, i.e., C (β-glucosidase activity), N (urease activity), P (acid phosphomonoesterase activity), S (acryl-sulfatase activity), and microbial activity (fluorescein diacetate hydrolysis and dehydrogenase activity) were investigated in As-contaminated soils of Ambagarh Chauki block, Chhattisgarh, Central India. The results revealed that the majority of the As in soils resided in the Fe/Mn oxide-bound fraction. The microbial biomass C, total and active fungal biomass, and enzyme activities were significantly inhibited by all the forms of As. However, water-soluble As, even though occupying only a small portion of the total As (0.9–2.9 %), exerted the greatest impact. Interestingly, total and active bacterial biomass was not significantly affected by As toxicity, suggesting their resistance to As. Urease activity was not affected by As pollution.

Lentiviral vectors are widely used as effective gene-delivery vehicles. Optimization of the conditions for efficient lentiviral transduction is of a high importance for a variety of research applications. Presence of positively charged polycations reduces the electrostatic repulsion forces between a negatively charged cell and an approaching enveloped lentiviral particle resulting in an increase in the transduction efficiency. Although a variety of polycations are commonly used to enhance the transduction with retroviruses, the relative effect of various types of polycations on the efficiency of transduction and on the potential bias in the determination of titer of lentiviral vectors is not fully understood. Here, we present data suggesting that DEAE-dextran provides superior results in enhancing lentiviral transduction of most tested cell lines and primary cell cultures. Specific type and source of serum affects the efficiency of transduction of target cell populations. Non-specific binding of enhanced green fluorescent protein (EGFP)-containing membrane aggregates in the presence of DEAE-dextran does not significantly affect the determination of the titer of EGFP-expressing lentiviral vectors. In conclusion, various polycations and types of sera should be tested when optimizing lentiviral transduction of target cell populations.

Iron slag, a byproduct of the steel manufacturing process with a high amount of iron (Fe), magnesium (Mg), manganese (Mn) and zinc (Zn), was used as a seed coating material to improve soybean nutrient quality and maintain yield during cultivation. Soybean yield (grain, aboveground, roots) did not differ significantly from the non-coated seeds, but nutrient concentration in soybeans, such as nitrogen, magnesium and manganese, were significantly increased in the iron-coated treatment, by 6%, 20% and 17%, respectively, than in the non-coated seeds. The application of iron slag as a protective seedcoat improved the nutrient concentrations of soybean seeds after harvest and maintained a good yield, implying that the material could be applied worldwide to improve the nutritional quality of soybeans in large scale production.

While cover crop residue (CR) incorporation offsets soil organic C (SOC) losses caused by plastic-film mulching (PFM), the microbial modulators and mechanisms of SOC accumulation remain poorly understood. Using functional gene microarray, soil enzyme activities, and soil density fractionation approaches, we investigated the mechanism, by incorporating CR (13.0 and 9 Mg ha−1 on a dry weight basis) under PFM in organic maize cropping systems, spanning 2 consecutive years. Compared to no-mulching, PFM without CR amendment significantly decreased SOC stock by 23%, corresponding to an increase in CO2 efflux by 74%, a decrease in light fraction C (LFOC) and heavy fraction C (HFOC) by 13 and 11%, respectively, and an increase in relative abundances of labile as well as recalcitrant C-degrading genes and related soil enzyme activities. However, PFM with CR amendment reduced the SOC stock loss to 11%, corresponding to 36% increase in CO2 efflux, 22% decrease in LFOC but 12% increase in HFOC, and an increase in relative abundances of labile C-degrading genes and related soil enzyme activities but a decrease in relative abundances of recalcitrant C-degrading genes and related soil enzyme activities. Our results, based on microbial functional genes, suggest that reduced degradation of recalcitrant C was responsible for increased mineral-associated C and thus SOC accumulation under PFM in organically cultivated maize.

Many waterbirds used to feed mainly on land but spend a considerable amount of time at waterbody for roosting. These waterbirds are known to bring a considerable amount of nutrients from terrestrial to the aquatic ecosystem with their faeces. In small waterbody, large congregations of waterbirds can cause huge nutrient loading to the lake. This sometimes may exceed 70% of the total lake nutrient budget. The present study intends to investigate the importance of waterbirds in terms of nutrient loading with faecal matter. The study was conducted at a shallow, eutrophic lake with high winter avian congregation and negligible anthropogenic nutrient input. Daily dropping production by individual waterbird, elemental concentration in dropping, individual species count and lake use pattern by waterbirds have been taken into consideration for the nutrient loading estimation by waterbird. Waterbirds were found to be the most important nutrient source to the lake, contributing about 89.5% of total nitrogen (N) and 89.9% of total phosphorous (P). Waterbirds contribute 370 kg year −1 (17.68 g m −2  year −1 ) and 67 kg year −1 (3.21 g m −2  year −1 ) of N and P, respectively, to the waterbody. Among waterbirds, Dendrocygna javanica was found to contribute the most amount of nutrients to the lake. Apart from waterbirds, precipitation was found to be the second important nutrient source to the lake. It was found that the lake receives most of the nutrients during winter due to high winter avian congregations. The present study concludes that waterbirds are the most important nutrient source for the studied lake. This study highlights the potential of waterbirds as a eutrophicating agent and future research should include it in eutrophication research if present at a high number. After all, this kind of study in the tropical/subtropical region is lacking and this study could put a light on the importance of waterbirds in this kind of system.