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is highly susceptible to vibriosis disease. Aims of the study were to identify the pathogen causing vibriosis in through molecular techniques and develop a biocontrol method of the disease by application of herbal extracts. Shrimp samples were collected aseptically from the infected farm and the bacteria were isolated from the infected region of those samples. Based on phenotypic identification, several isolates were identified as sp. 16S rRNA gene sequences of the selected isolates exhibited 100% homology with strain ATCC 17749. An infection challenge test was performed by immersion method with where these isolates caused high mortality in juvenile shrimp with prominent symptoms of hepatopancreatic necrosis. Antibiogram profile of the isolates was determined against eleven commercial antibiotic discs whereas the isolates were found resistant to multiple antibiotics. A total of twenty-one herbal extracts were screened where , and strongly inhibited the growth of in conditions. In conditions, the ethyl acetate extracts of and successfully controlled the vibriosis disease in shrimp at a dose of 10 mg/g feed. This is the first report on molecular identification and biocontrol of in shrimp in Bangladesh.

The opportunistic fish pathogen, Enterococcus faecalis has been reported to cause mass mortality in several fish species in different countries. The objectives of this study were to (i) identify E . faecalis from the diseased fishes through molecular techniques; (ii) assess the antibiotic susceptibility profile of E . faecalis isolates; and (iii) control disease in tilapia fish by treatment with medicinal plant extracts. A total of 48 isolates were phenotypically identified as Enterococcus species from tilapia, stinging catfish and walking catfish cultivated in several fish farms in Gazipur. Ten randomly selected isolates were identified as E. faecalis by 16S rRNA gene sequencing. Artificial infection revealed that most of the isolates caused moderate to high mortality in fishes with characteristic disease symptoms. These isolates exhibited resistance to multiple antibiotics in vitro . Bioassay revealed that organic extracts of Tamarindus indica and Emblica officinalis leaves, Allium sativum bulb, and Syzygium aromaticum bud inhibited the growth of E . faecalis . Methanol extracts of A . sativum and methanol and acetone extracts of S . aromaticum significantly reduced the mortality of fish artificially infected with E . faecalis as both preventive and therapeutic agents. This is the first report on molecular identification, and herbal control of fish pathogenic E . faecalis in Bangladesh.