Explore the vast range of titles available.

BACKGROUND: Balanced non-robertsonian translocation (RT), involving acrocentric chromosomes, is a rare event and only a few cases are reported. Most of the RTs are balanced involving acrocentric chromosomes with the breakpoints (q10;q10). MATERIALS AND METHODS: Chromosome analysis was performed as per standard procedure - Giemsa-trypsin banding with 500 band resolution was analyzed for chromosome identification. RESULTS: In the present study, we report a rare balanced non-RTs involving chromosomes 13 and 15 with cytogenetic finding of 46, XX, t(13;15) (p11.2;q22.1). CONCLUSION: To the best of our knowledge, this is the first such report of an unusual non-RT of t(13:15) with (p11.2;q22.1) break points.

Digital ischemia with gangrene is a rare manifestation of chronic myeloid leukemia (CML). We report a rare CML case with severe thrombocytosis presenting clinically as digital ischemia and gangrene, the sole primary symptom. A 40-year-old patient presented with pain and discoloration of the tips of the ring and little fingers of the right hand for 20 days, which later progressed to dry gangrene with a line of demarcation and pre-gangrenous changes in the index and middle fingers. He was a chronic smoker and alcoholic with no other comorbidities. On investigation, he was found to have abnormal leukocytosis (153,000/mm ) and moderate thrombocytosis (969,000/mm ) with anemia. In view of abnormal leukocytosis, he was investigated for leukemia. He was found to be positive for BCR-ABL by reverse transcription polymerase chain reaction (RT-PCR), thus confirming the diagnosis of CML. He received imatinib 400 mg/day and subsequently experienced resolution of symptoms and complete hematological response by the 12th week of therapy.

Purpose Emerging evidence clearly suggests the potential chemopreventive and anti-tumor activity of a well known “natural agent” curcumin. However, studies have shown that curcumin is not readily bioavailable, and thus the tissue bioavailability of curcumin is also poor except for gastrointestinal track. Because of the potential biological activity of curcumin, many studies have attempted for making a better analog of cucumin that is equally effective or better with increased bioavailability, which was the purpose of our current study. Methods We have designed and synthesized new difluoro Knoevenagel condensates of curcumin and Schiff bases along with their copper (II) complexes and evaluated their biological activities with respect to the inhibitory effects on purified rabbit 26S proteasome, and growth inhibition and induction of apoptosis in colon and pancreatic cancer cell lines. Results All copper complexes possess distorted square planar geometries with 1:1 metal to ligand stoichiometry with reversible copper redox couple. The difluoro compound CDF exhibited inhibitory effects on purified rabbit 20S proteasome or cellular 26S proteasome, and caused both growth inhibition of cancer cell lines and induced apoptotic cell death in our preliminary assessment. Conclusion Our results suggest that our newly synthesized classes of curcumin analogs could be useful as chemopreventive and/or therapeutic agents against cancers.

Purpose The purpose of the current study was to assess the effect of newly synthesized Curcumin analogs on COX-2 protein by molecular docking studies and by assessments of the effect of one such analog (CDF) on nuclear factor NF-κB and PGE₂. In addition, we have determined the pharmacokinetics and tissue distribution of CDF in mice compared to Curcumin. Methods Molecular docking on COX-2 protein was assessed by standard computer modeling studies. PGE₂ assay in conditioned media was done utilizing high sensitivity immunoassay kit following manufacturer's instructions, while NF-κB was done by routine EMSA. Serum pharmacokinetics and tissue distribution studies were carried out using the validated high-performance liquid chromatography with tandem mass spectrometry (LC-MS/MS) methods. Results The molecular docking showed that fluorocurcumin analogs do not introduce any major steric changes compared to the parent Curcumin molecule, which was consistent with down-regulation of NF-κB and reduced PGE₂ levels in cells treated with CDF. Pharmacokinetic parameters revealed that CDF had better retention and bioavailability and that the concentration of CDF in the pancreas tissue was 10-fold higher compared to Curcumin. Conclusion Our observations clearly suggest that the bioavailability of CDF is much superior compared to Curcumin, suggesting that CDF would be clinically useful.

Balanced non-robertsonian translocation (RT), involving acrocentric chromosomes, is a rare event and only a few cases are reported. Most of the RTs are balanced involving acrocentric chromosomes with the breakpoints (q10;q10). Chromosome analysis was performed as per standard procedure - Giemsa-trypsin banding with 500 band resolution was analyzed for chromosome identification. In the present study, we report a rare balanced non-RTs involving chromosomes 13 and 15 with cytogenetic finding of 46, XX, t(13;15) (p11.2;q22.1). To the best of our knowledge, this is the first such report of an unusual non-RT of t(13:15) with (p11.2;q22.1) break points.

BACKGROUNDBalanced non-robertsonian translocation (RT), involving acrocentric chromosomes, is a rare event and only a few cases are reported. Most of the RTs are balanced involving acrocentric chromosomes with the breakpoints (q10;q10).MATERIALS AND METHODSChromosome analysis was performed as per standard procedure - Giemsa-trypsin banding with 500 band resolution was analyzed for chromosome identification.RESULTSIn the present study, we report a rare balanced non-RTs involving chromosomes 13 and 15 with cytogenetic finding of 46, XX, t(13;15) (p11.2;q22.1).CONCLUSIONTo the best of our knowledge, this is the first such report of an unusual non-RT of t(13:15) with (p11.2;q22.1) break points.

Obesity is a consequence of a complex interplay between the host genome and the prevalent obesogenic factors among the modern communities. The role of gut microbiota in the pathogenesis of the disorder was recently discovered; however, 16S-rRNA-based surveys revealed compelling but community-specific data. Considering this, despite unique diets, dietary habits and an uprising trend in obesity, the Indian counterparts are poorly studied. Here, we report a comparative analysis and quantification of dominant gut microbiota of lean, normal, obese and surgically treated obese individuals of Indian origin. Representative gut microbial diversity was assessed by sequencing fecal 16S rRNA libraries for each group (n = 5) with a total of over 3000 sequences. We detected no evident trend in the distribution of the predominant bacterial phyla, Bacteroidetes and Firmicutes. At the genus level, the bacteria of genus Bacteroides were prominent among the obese individuals, which was further confirmed by qPCR (P < 0.05). In addition, a remarkably high archaeal density with elevated fecal SCFA levels was also noted in the obese group. On the contrary, the treated-obese individuals exhibited comparatively reduced Bacteroides and archaeal counts along with reduced fecal SCFAs. In conclusion, the study successfully identified a representative microbial diversity in the Indian subjects and demonstrated the prominence of certain bacterial groups in obese individuals; nevertheless, further studies are essential to understand their role in obesity.

In the present study, self-assembled hierarchical CoMn-LDH, CoMn@CuZnS, and CoMn@CuZnFeS heterostructured composites were synthesized for bifunctional applications. As an electrode for a supercapacitor, CoMn-LDH demonstrated superior areal and specific capacitance of 5.323 F cm−2 (279.49 mAh/g) at 4 mA cm−2, comparable to or even higher than other LDHs. The assembled AC//CoMn-LDH hybrid supercapacitor device further demonstrated better stability with 63% original capacitance over 20,000 cycles. Later, as a catalyst, CoMn-LDH, CoMn@CuZnS, and CoMn@CuZnFeS electrodes revealed better performance, with overpotentials of 340, 350, and 366 and −199, −215, and −222 mV to attain 10 mA cm−2 of current density for the oxygen evolution reaction (OER) and hydrogen evolution reaction (HER), respectively. Moreover, for CoMn-LDH, small Tafel slopes of 102 and 128 mV/dec were noticed for OER and HER with good stability compared to heterostructured electrodes.

The verification of mathematical models for multistage reciprocating compressors is crucial for ensuring their accuracy and reliability. In this study, we used different machine learning (ML) models to verify the results of MATLAB-based models of single-stage reciprocating compressors, multistage reciprocating compressors without intercoolers, and multistage reciprocating compressors with intercoolers to simulate the real-world operating conditions of a reciprocating compressor. The verification focuses on key performance indicators, such as the pressure–volume (PV) graph, outlet temperature graph, volumetric efficiency, and pressure ratio graph. The MATLAB model computes thermodynamic parameters, such as the power required, outlet pressure, and outlet temperature for various operating conditions. The MATLAB model produced the following results for single-stage compressor: the outlet pressure increased by 1.6 times the inlet pressure of the compressor, the volume reduced by 20% of the volume at the inlet of the single-stage compressor, and the outlet temperature increased by 30% of the inlet temperature. In the case of a multistage compressor without an intercooler, the outlet pressure increased by about 3.3–3.6 times the inlet pressure of the compressor; the volume reduced by 60% of the volume at the inlet, and the outlet temperature increased by 35% in comparison to the inlet temperature of the multistage compressor without an intercooler. Subsequently, in the case of a multistage compressor with an intercooler at the first stage of compression, the pressure increased by three times the inlet pressure; at the second stage of compression, the pressure increased by six times the inlet pressure of the compressor, the volume was reduced by approximately 80%, and the intercooler maintained the increase in outlet temperature by 30%, limiting it and preventing excessive expansion of air in the compressor and increasing the efficiency of the compressor by 12% in comparison to the multistage compressor without an intercooler. In addition, the results generated by all the machine learning models used in the study were in correlation with the results generated by the MATLAB model for all three compressors, with an accuracy of approximately 90% or more for almost all the models implemented for prediction. By comparing the predicted outputs from the ML model with the MATLAB-generated results, the accuracy and consistency of the simulation were assessed. This study aims to bridge the gap between traditional mathematical modeling and modern data-driven validation techniques to ensure robustness in compressor performance predictions.

Tricholepis glaberrima commonly known as Potato weed widely used in traditional system of medicine for the treatment of diabetes mellitus In the present study, Methanol extract of Tricholepis glaberrima (METG) leaves were subjected to phytochemical investigation and evaluated for antidiabetic activity in STZ induced diabetic rats. METG (100, 200mg/kg) and Glibenclamide (5mg/kg) were administered orally in STZ (60mg/kg, i.p.) induced diabetic rats. In acute oral toxicity (OECD Guide line 423) study, administration of METG no mortality upto 1000mg/kg was observed. OGTT, fasting blood glucose level, body weight, plasma insulin, were evaluated in normal and diabetic rats. preliminary phytochemical investigation revealed the presence of alkaloids, flavonoids, glycosides, tannins as the major constituents in the methanol extract of Tricholepis glaberrima. These results suggest that METG (200mg/kg) showed antihyperglycemic activity in STZ induced diabetic rats.