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Background Motor symptoms of spinal cord injury (SCI) considerably impair quality of life and are associated with a high risk of secondary diseases. So far, no pharmacological treatment is available for these symptoms. Therefore, we conducted a randomized, double‐blinded, placebo‐controlled study in dogs with spontaneous SCI due to disc herniation to test whether a reduction of spinal inhibitory activity by intramuscular injections of tetanus neurotoxin (TeNT) alleviates motor symptoms such as muscle atrophy or gait function. Methods To this end, 25 dogs were treated with injections of either TeNT or placebo into their paretic hindlimb muscles. Effects of TeNT on muscle thickness were assessed by ultrasound, while effects on gait function were measured using the modified functional scoring system in dogs. Results Four weeks after the TeNT injections, muscle thickness of the gluteus medius muscle (before median 1.56 cm [inter‐quartile range IQR 1.34–1.71 cm] and after median 1.56 cm [IQR 1.37–1.85 cm], P‐value 0.0133) as well as of the rectus femoris muscle (before median 0.76 cm [IQR 0.60–0.98 cm] and after median 0.93 cm [IQR 0.65–1.05 cm], P‐value 0.0033) significantly increased in the TeNT group. However, there was no difference in gait function between the TeNT and placebo groups. The treatment was well tolerated by all dogs without any signs of generalized tetanus symptoms or any spreading of effects beyond the lumbar level of the injected hindlimbs. Conclusions With regard to the beneficial effects on muscle thickness, intramuscular injections of TeNT represent the first pharmacological approach that focally reverses muscle atrophy in SCI. Moreover, the study data support the safety of this treatment when TeNT is used at low dose.

Study designCase series on four dogs.ObjectivesTo determine the alleviation of motor symptoms in spinal cord injury (SCI) by tetanus neurotoxin (TeNT).SettingDifferent Berlin veterinary clinics, Germany.MethodsWe report on the effect of intramuscular injections of low-dose TeNT into paretic hind limb muscles 2–157 weeks after SCI due to lumbar disc herniation in a clinical case series on four dogs. All dogs underwent unsuccessful or incomplete surgical decompression prior to TeNT treatment. TeNT was injected on a compassionate basis. Stance, gait ability and the diameter of the rectus femoris muscle were assessed as parameters.ResultsAll four dogs improved their stance and three of these dogs improved in gait at 4 and 6 weeks after TeNT injections without evidence of side effects or spreading of TeNT effects. At the same time, the size of the rectus femoris muscle diameter increased considerably as compared with baseline (baseline: 100%; 4 weeks: 148.7% ± 10.9%; 6 weeks: 137.1% ± 7.9%).ConclusionsFacilitation of α-motor neurons by TeNT injections into paretic hind limb muscles of four dogs improved standing and/or gait abilities and partly reversed muscle atrophy after SCI. The absence of generalized or painful muscle spasms supports the safety of low-dose TeNT. Therefore, TeNT might evolve as a promising therapeutic option for muscle paresis of central origin, e.g. in individuals with SCI, stroke or multiple sclerosis.

There is a paucity of information on the clinical course and outcome of young cats with polyneuropathy. The aim of the study was to describe the clinical features, diagnostic investigations, and outcome of a large cohort of cats with inflammatory polyneuropathy from several European countries. Seventy cats with inflammatory infiltrates in intramuscular nerves and/or peripheral nerve biopsies were retrospectively included. Information from medical records and follow up were acquired via questionnaires filled by veterinary neurologists who had submitted muscle and nerve biopsies (2011–2019). Median age at onset was 10 months (range: 4–120 months). The most common breed was British short hair (25.7%), followed by Domestic short hair (24.3%), Bengal cat (11.4%), Maine Coon (8.6%) and Persian cat (5.7%), and 14 other breeds. Male cats were predominantly affected (64.3%). Clinical signs were weakness (98.6%) and tetraparesis (75.7%) in association with decreased withdrawal reflexes (83.6%) and, less commonly, cranial nerve signs (17.1%), spinal pain/hyperesthesia (12.9%), and micturition/defecation problems (14.3%). Onset was sudden (30.1%) or insidious (69.1%), and an initial progressive phase was reported in 74.3%. Characteristic findings on electrodiagnostic examination were presence of generalized spontaneous electric muscle activity (89.6%), decreased motor nerve conduction velocity (52.3%), abnormal F-wave studies (72.4%), pattern of temporal dispersion (26.1%) and unremarkable sensory tests. The clinical course was mainly described as remittent (49.2%) or remittent-relapsing (34.9%), while stagnation, progressive course or waxing and waning were less frequently reported. Relapses were common and occurred in 35.7% of the cats' population. An overall favorable outcome was reported in 79.4% of patients. In conclusion, young age at the time of diagnosis and sudden onset of clinical signs were significantly associated with recovery ( p < 0.05). Clinical and electrodiagnostic features and the remittent-relapsing clinical course resembles juvenile chronic inflammatory demyelinating polyneuropathy (CIDP), as seen in human (children/adolescents), in many aspects.

Inflammatory polyradiculoneuropathy (IMPN) is one of the causes of sudden onset of neuromuscular signs such as para-/tetraparesis in young cats. Even though most cases have a favorable outcome, persistent deficits, relapses, and progressive courses are occasionally seen. As clinical presentation does not always appear to predict outcome and risk of recurrence, this study was initiated to screen for prognostic biopsy findings in a large cohort of histologically confirmed IMPN cases with clinical follow-up. In total, nerve and muscle specimens of 107 cats with biopsy diagnosis of presumed autoreactive inflammatory polyneuropathy and 22 control cases were reviewed by two blinded raters for a set of 36 histological parameters. To identify patterns and subtypes of IMPN, hierarchical k-means clustering of 33 histologic variables was performed. Then, the impact of histological parameters on IMPN outcome was evaluated via an univariate analysis to identify variables for the final multivariate model. The data on immediate outcome and follow-up were collected from submitting neurologists using a purpose-designed questionnaire. Hierarchical k-means clustering sorted the tissues into 4 main categories: cluster 1 (44/129) represents a purely inflammatory IMPN picture, whereas cluster 2 (47/129) was accompanied by demyelinating features and cluster 3 (16/129) by Wallerian degeneration. Cluster 4 (22/129) reflects normal tissues from non-neuropathic control cats. Returned questionnaires provided detailed information on outcome in 63 animals. They were categorized into recovered and non-recovered. Thereby, fiber-invasive infiltrates by mononuclear cells and mild fiber loss in intramuscular nerve branches correlated with higher probabilities of recovery. Remyelination in semithin sections, on the other hand, is correlated with a less favorable outcome. Animals grouping in cluster 1 had a tendency to a higher probability of recovery compared to other clusters. In conclusion, diagnosis of feline IMPN from nerve and muscle biopsies allowed for the identification of histologic features that were positively or negatively correlated with outcome.

Sedimentary features can significantly influence flow and transport pathways in fine-grained valley fills. The spatial extent and properties of these features should be analyzed in order to determine residence times, flow pathways, and the reaction potential for contaminants. In the Ammer floodplain close to Tübingen, we examined peat layers and a gravel channel in order to determine their influence on the regional hydrogeology and hydrochemistry. Towards this end, we selected and combined geophysical and hydrogeological investigation techniques. Surface geoelectrical measurements revealed the spatial extent of relevant features, while subsequent borehole geophysical and direct-push surveys, including the in-situ detection of the sediment color, were used to assess their geometry and internal heterogeneity. At representative points, the hydraulic and biogeochemical properties of the sediments and the groundwater could be determined by targeted sampling and hydraulic tests. The general methodology of delineating relevant sub-units followed by high-resolution profiling may also be applied to investigate larger-scale valley fills.

It's time for sustainable, environmental performance The Common Agricultural Policy (CAP) of the European Union (EU) is one of the world's largest agricultural policies and the EU's longest-prevailing one. Originally focused mostly on supporting production and farm income, the CAP has progressively integrated instruments to support the environment. Nonetheless, there is considerable agreement among EU citizens that the CAP still does not do enough to address ongoing environmental degradation and climate change (92% of nonfarmers, 64% of farmers) ( 1 ). In May and June 2018, the European Commission (EC) published the financial plan and legislative proposal for the CAP post-2020 ( 2 ), prompting numerous proposed amendments that the newly elected European Parliament (EP) will now have to consider. With an eye toward the next and final reform stages, including budget discussions and “trilogue” negotiations between the EC, the Council, and the EP to begin in autumn 2019, we examine whether the proposed post-2020 CAP can address key sustainability issues and meet societal demands for higher environmental performance.

In Europe and, increasingly, the rest of the world, the key policy tool for the control of air pollution is the critical load, a level of pollution below which there are no known significant harmful effects on the environment. Critical loads are used to map sensitive regions and habitats, permit individual polluting activities, and frame international negotiations on transboundary air pollution. Despite their fundamental importance in environmental science and policy, there has been no systematic attempt to verify a critical load with field survey data. Here, we use a large dataset of European grasslands along a gradient of nitrogen (N) deposition to show statistically significant declines in the abundance of species from the lowest level of N deposition at which it is possible to identify a change. Approximately 60% of species change points occur at or below the range of the currently established critical load. If this result is found more widely, the underlying principle of no harm in pollution policy may need to be modified to one of informed decisions on how much harm is acceptable. Our results highlight the importance of protecting currently unpolluted areas from new pollution sources, because we cannot rule out ecological impacts from even relatively small increases in reactive N deposition.

The Radar Vegetation Index (RVI) is a well-established microwave metric of vegetation cover. The index utilizes measured linear scattering intensities from co- and cross-polarization and is normalized to ideally range from 0 to 1, increasing with vegetation cover. At long wavelengths (L-band) microwave scattering does not only contain information coming from vegetation scattering, but also from soil scattering (moisture & roughness) and therefore the standard formulation of RVI needs to be revised. Using global level SMAP L-band radar data, we illustrate that RVI runs up to 1.2, due to the pre-factor in the standard formulation not being adjusted to the scattering mechanisms at these low frequencies. Improvements on the RVI are subsequently proposed to obtain a normalized value range, to remove soil scattering influences as well as to mask out regions with dominant soil scattering at L-band (sparse or no vegetation cover). Two purely vegetation-based RVIs (called RVII and RVIII), are obtained by subtracting a forward modeled, attenuated soil scattering contribution from the measured backscattering intensities. Active and passive microwave information is used jointly to obtain the scattering contribution of the soil, using a physics-based multi-sensor approach; simulations from a particle model for polarimetric vegetation backscattering are utilized to calculate vegetation-based RVI-values without any soil scattering contribution. Results show that, due to the pre-factor in the standard formulation of RVI the index runs up to 1.2, atypical for an index normally ranging between zero and one. Correlation analysis between the improved radar vegetation indices (standard RVI and the indices with potential improvements RVII and RVIII) are used to evaluate the degree of independence of the indices from surface roughness and soil moisture contributions. The improved indices RVII and RVIII show reduced dependence on soil roughness and soil moisture. All RVI-indices examined indicate a coupled correlation to vegetation water content (plant moisture) as well as leaf area index (plant structure) and no single dependency, as often assumed. These results might improve the use of polarimetric radar signatures for mapping global vegetation.

Fungal communities play a key role in ecosystem functioning. However, only little is known about their composition in plant roots and the soil of biomass plantations. The goal of this study was to analyze fungal biodiversity in their belowground habitats and to gain information on the strategies by which ectomycorrhizal (ECM) fungi form colonies. In a 2-year-old plantation, fungal communities in the soil and roots of three different poplar genotypes (Populus × canescens, wildtype and two transgenic lines with suppressed cinnamyl alcohol dehydrogenase activity) were analyzed by 454 pyrosequencing targeting the rDNA internal transcribed spacer 1 (ITS) region. The results were compared with the dynamics of the root-associated ECM community studied by morphotyping/Sanger sequencing in two subsequent years. Fungal species and family richness in the soil were surprisingly high in this simple plantation ecosystem, with 5944 operational taxonomic units (OTUs) and 186 described fungal families. These findings indicate the importance that fungal species are already available for colonization of plant roots (2399 OTUs and 115 families). The transgenic modification of poplar plants had no influence on fungal root or soil communities. Fungal families and OTUs were more evenly distributed in the soil than in roots, probably as a result of soil plowing before the establishment of the plantation. Saprophytic, pathogenic, and endophytic fungi were the dominating groups in soil, whereas ECMs were dominant in roots (87%). Arbuscular mycorrhizal diversity was higher in soil than in roots. Species richness of the root-associated ECM community, which was low compared with ECM fungi detected by 454 analyses, increased after 1 year. This increase was mainly caused by ECM fungal species already traced in the preceding year in roots. This result supports the priority concept that ECMs present on roots have a competitive advantage over soil-localized ECM fungi.

Local vegetation trends in the Sahel of Mali and Senegal from Geoland Version 1 (GEOV1) (5 km) and the third generation Global Inventory Modeling and Mapping Studies (GIMMS3g) (8 km) Fraction of Absorbed Photosynthetically Active Radiation (FAPAR) time series are studied over 29 years. For validation and interpretation of observed greenness trends, two methods are applied: (1) a qualitative approach using in-depth knowledge of the study areas and (2) a quantitative approach by time series of biomass observations and rainfall data. Significant greening trends from 1982 to 2010 are consistently observed in both GEOV1 and GIMMS3g FAPAR datasets. Annual rainfall increased significantly during the observed time period, explaining large parts of FAPAR variations at a regional scale. Locally, GEOV1 data reveals a heterogeneous pattern of vegetation change, which is confirmed by long-term ground data and site visits. The spatial variability in the observed vegetation trends in the Sahel area are mainly caused by varying tree- and land-cover, which are controlled by human impact, soil and drought resilience. A large proportion of the positive trends are caused by the increment in leaf biomass of woody species that has almost doubled since the 1980s due to a tree cover regeneration after a dry-period. This confirms the re-greening of the Sahel, however, degradation is also present and sometimes obscured by greening. GEOV1 as compared to GIMMS3g made it possible to better characterize the spatial pattern of trends and identify the degraded areas in the study region.