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Transcriptome-wide survey divulged a total of 181 ABC transporters in G. glabra which were phylogenetically classified into six subfamilies. Protein–Protein interactions revealed nine putative GgABCBs (-B6, -B14, -B15, -B25, -B26, -B31, -B40, -B42 &-B44) corresponding to five AtABCs orthologs (-B1, -B4, -B11, -B19, &-B21). Significant transcript accumulation of ABCB6 (31.8 folds), - B14 (147.5 folds), - B15 (17 folds), -B25 (19.7 folds), - B26 (18.31 folds), -B31 (61.89 folds), -B40 (1273 folds) and - B42 (51 folds) was observed under the influence of auxin. Auxin transport-specific inhibitor, N-1-naphthylphthalamic acid, showed its effectiveness only at higher (10 µM) concentration where it down regulated the expression of ABCBs , PINs (PIN FORMED) and TWD1 (TWISTED DWARF 1) genes in shoot tissues, while their expression was seen to enhance in the root tissues. Further, qRT-PCR analysis under various growth conditions ( in-vitro , field and growth chamber), and subjected to abiotic stresses revealed differential expression implicating role of ABCBs in stress management. Seven of the nine genes were shown to be involved in the stress physiology of the plant. GgABCB6 , 15 , 25 and ABCB31 were induced in multiple stresses, while GgABCB26 , 40 & 42 were exclusively triggered under drought stress. No study pertaining to the ABC transporters from G. glabra is available till date. The present investigation will give an insight to auxin transportation which has been found to be associated with plant growth architecture; the knowledge will help to understand the association between auxin transportation and plant responses under the influence of various conditions.

Understanding the population trends and seasonal fluctuations of the Indian flying fox, Pteropus medius , is crucial for developing effective conservation strategies. This study investigates these trends and fluctuations at eight permanent roosting sites within the semi-arid habitat of Kurukshetra district, Haryana, over two periods: April 2021–March 2022 and October 2022–March 2023. Significant monthly population fluctuations were observed, with peak colony sizes during the mating (September–November) and parturition periods (March–April), and the lowest populations during the peak summer months (May–July). There were significant differences ( P ≤ 0.05) in mean population sizes between seasons and roosting sites. However, there were no significant differences ( P ≥ 0.05) in mean population size between the two study years. Vijay Colony had the highest average population size, while Jyotisar had the lowest. Population fluctuations showed a negative correlation with ambient temperature ( r = –0.113, p ≥ 0.05; r = –0.069, p ≥ 0.05) and precipitation ( r = –0.117, p ≥ 0.05; r = –0.202, p ≤ 0.05), and a positive correlation with humidity ( r = 0.123, p ≥ 0.05; r = 0.669, p ≤ 0.05). Additionally, the study identified significant threats to P. medius , including habitat destruction from tree cutting for commercial purposes, habitat disturbances from human activities, electrocution from power lines, and extreme summer heat. These findings highlight the urgent need for targeted conservation measures to mitigate these threats and protect the P. medius population in the study area.

To achieve appropriate interaction rates in recent neutrino oscillation studies, high atomic number nuclear targets were utilized. Because of the nuclear effects in the experimental observable, the utilization of these complicated targets produced systematic uncertainties that needed to be assessed accurately to constrain the discovery. We made an effort to calculate the nuclear effects in the Ar and H targets, which are intended to be employed at the DUNE far and near detectors, respectively, through our simulation effort. The DUNE flux is peaking around 2.5 GeV, and CCRES is the dominant process at this energy. So, this work will be focused only on the CCQE and CCRES interactions and the simulations will be done using two different neutrino event generators. We reported the ratio of the oscillation probability (P(Ar)/P(H) as a function of reconstructed neutrino energy for CCRES channels to quantify the systematic errors in the observables.

An account of eight species of genus Hyphoderma (H. clavatum, H. definitum, H. echinocystis, H. litschaueri, H. nemorale, H. subpraetermissum, H. tibia, and H. transiens) is presented, which is based on collections made from Uttarakhand state during 2009–2014. All these species are cited and fully described for the first time from India.

The recent advancements in sequencing technologies and informatic tools promoted a paradigm shift to decipher the hidden biological mysteries and transformed the biological issues into digital data to express both qualitative and quantitative forms. The transcriptomic approach, in particular, has added new dimensions to the versatile essence of plant genomics through the large and deep transcripts generated in the process. This has enabled the mining of super families from the sequenced plants, both model and non-model, understanding their ancestry, diversity, and evolution. The elucidation of the crystal structure of the WRKY proteins and recent advancement in computational prediction through homology modeling and molecular dynamic simulation has provided an insight into the DNA–protein complex formation, stability, and interaction, thereby giving a new dimension in understanding the WRKY regulation. The present review summarizes the functional aspects of the high volume of sequence data of WRKY transcription factors studied from different species, till date. The review focuses on the dynamics of structural classification and lineage in light of the recent information. Additionally, a comparative analysis approach was incorporated to understand the functions of the identified WRKY transcription factors subjected to abiotic (heat, cold, salinity, senescence, dark, wounding, UV, and carbon starvation) stresses as revealed through various sets of studies on different plant species. The review will be instrumental in understanding the events of evolution and the importance of WRKY TFs under the threat of climate change, considering the new scientific evidences to propose a fresh perspective.

Main conclusion ABCs transport diverse compounds; with plant’s most abundant ABCG and ABCB subfamilies. ABCBs are multi-functional transporter proteins having role in plant adaptation. ATP-binding cassette (ABC) proteins have been known for the transportation of various structurally diverse compounds in all kingdoms of life. Plants possess a particularly high number of ABC transporters compared to other eukaryotes: the most abundant being ABCG followed by the ABCB subfamilies. While members of the ABCB subfamily are primarily known for auxin transportation, however, studies have shown their involvement in variety of other functions viz . growth and development, biotic and abiotic stresses, metal toxicity and homeostasis, cellular redox state stability, stomatal regulation, cell shape maintenance, and transport of secondary metabolites and phytohormones. These proteins are able to perform various biological processes due to their widespread localization in the plasma membrane, mitochondrial membrane, chloroplast, and tonoplast facilitating membrane transport influenced by various environmental and biological cues. The current review compiles published insights into the role of ABCB transporters, and also provides brief insights into the role of ABCB transporters in a medicinal plant, where the synthesis of its bioactive secondary metabolite is linked to the primary function of ABCBs, i.e., auxin transport. The review discusses ABCB subfamily members as multi-functional protein and comprehensively examines their role in various biological processes that help plants to survive under unfavorable environmental conditions. Graphical abstract

Even though breast milk is the universally accepted infant feeding method, practices of pre lacteal feeding, late initiation of breast milk, early initiation of complementary feeding are still prevalent in many countries including India. This study assessed mothers’ awareness, attitudes and practices regarding exclusive breast feeding up to first six months of infancy. Breastfeeding yields important long-term health benefits for infants and their mothers, including positive impact on children's cognitive development and well-being. Workplace barriers contribute to low rates ofbreastfeeding. Employment of mothers outside home, especially full-time employment has a negative influence on duration of breastfeeding. Women frequently attribute early weaning to unsupportive work environments. Lack of privacy and adequate time to express breast milk are cited as barriers. Other limitations include employers' perception that the presence of infants in the workplace reduces mothers' productivity, regulations and other rules that bar children from the workplace, and a lack of child care close to the workplace. Field recording on infant’s clinical assessments, anthropometric measurements and immunization schedule family behaviours was carried out.

Plumbagin is the major secondary metabolite found in Plumbago species, it exhibit a wide variety of pharmaceutical activities. The initial focus of this study was to establish a suitable protocol for direct shoot regeneration from nodal explants on Murashige and Skoog (MS) medium fortified with 6-benzylaminopurine (BAP) and α-naphthalene-acetic acid (NAA). Single shoot regenerated from the nodal explants when cultured on BAP alone, whereas multiple shoots regenerated with BAP and NAA. The maximum number of shoots were obtained on medium fortified with 5.5 µM BAP and 3.0 µM NAA. Furthermore, the shoot cultures were treated with biotic elicitors (lysates of Agrobacterium rhizogenes and Trichoderma viride and yeast extract). The application of elicitors was optimized by the Response Surface Methodology coupled with Box-Behnken design (RSM-BBD). The experimental and predicted values were analyzed and the elicitors were found to be stastically significant. Plumbagin was quantified by liquid chromatography/electrospray ionization tandem. The highest plumbagin yield was found to be 18-fold higher as compared to the control when treated with 1.5% A. rhizogenes lysate, 2.25% T. viride lysate and 2% yeast extract. The best model for optimum production of plumbagin was experimentally verified and the correlation between the predicted (1.751%) and actual values (1.732%) was found to be 98.7% for plumbagin production. The present study suggest that the elicitors used enhance the production of plumbagin considerably. It is the first ever study to report the successful utilization of lysates enhancing plumbagin production in the shoot cultures.Key messageIn vitro production of plumbagin from Plumbago zeylenica. Analysis of plumbagin by LC-MS. Application of RSM to optimize the enhanced plumbagin yield by using eliciters.

A laboratory experiment was conducted to study the degradation dynamics of halosulfuron-methyl residues in sandy loam and clay loam soil. The herbicide formulation was applied at 0.034 and 0.068 mg kg− 1 equivalent to field application dose of 67.5 and 135 g a.i. ha− 1 as single and double dose respectively. Soil samples were collected on 0 (1 h), 1, 3, 7, 10, 15, 30 and 45 days after treatments. Extraction was done using modified QuEChERS method. Residues were estimated by UPLC coupled with quadrupole Dalton mass detector. Average recoveries ranged from 85.5% to 94.5% for both soils at different fortification levels of 0.005 to 0.1 mg kg− 1 with limit of detection (LOD) and limit of quantification (LOQ) as 0.001 and 0.005 mg kg− 1, respectively. Dissipation followed first order kinetics with half-life of 8.4 to 10.7 days in both soil at two doses. The residues reached below LOQ of 0.005 mg kg− 1 after 45 days of herbicide application.

Understanding the pion generation and the consequences of final-state interactions (FSI) are critical for the data processing in all neutrino experiments. The energy utilized in modern neutrino research of the resonance (RES) generation processes contributes significantly to the pion production. If a pion is absorbed in the nuclear matter after its production, the event may become unrecognizable from a quasielastic (QE) scattering process and act as a background. For oscillation experiments, estimating this background is critical, and it necessitates solid theoretical models for both pion generation at the primary vertex and after FSI. The number of pions created after FSI differs greatly from the number produced at the primary vertex due to FSI. Because neutrino detectors can only detect final-state particles, FSI obscures the proper information about particles created at the primary vertex. A detailed study of FSI is required to overcome this problem, which theoretical models incorporated in Monte Carlo (MC) neutrino event generators can provide. They should give theoretical results concerning the neutrino interactions for various researches, acting as a connection among both theoretical models and experimental data. In this paper, we provide simulated events for the pion creation in {\\nu} {\\mu} charge current (CC) interactions on a 40 Ar target in the Deep Underground Neutrino Experiment (DUNE) setup for two distinct MC generators: GENIE and NuWro. In comparison to GENIE (v-3.00.06), NuWro (v-19.02.2) is more opaque (less responsive) to the charge exchange and absorption processes; pions are more likely to be absorbed than produced during the intranuclear transport.