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On 6 April 2009, a moderate earthquake (Mw = 6.3; Ml = 5.8) struck the Abruzzo region in central Italy, causing more than 300 fatalities and heavy damage to L'Aquila and surrounding villages. Coseismic surface effects have been thoroughly documented by timely field surveys as well as by remote sensing analyses of satellite images. The outstanding quality of geological, seismological, geodetic, and interferometric synthetic aperture radar (InSAR) information arguably represents the best ever data set made available immediately after a moderate seismic event. Based on this data set, we aim at testing the capability of coupled geological and InSAR data to map surface faulting patterns associated with moderate earthquakes. Coseismic ground ruptures have been mapped at a scale of 1:500 in the whole epicentral area. Traces of surface ruptures have been inferred from linear phase discontinuities identified in the interferogram. A very good agreement between the two methods resulted in the characterization of the main surface rupture along the Paganica fault. The same approach applied to ground ruptures hypothesized along other capable fault segments provided more questionable results. Thus, the combined field and InSAR approach appeared useful for detecting continuous surface ruptures exceeding 1 km in length and showing displacements greater than a few centimeters. These are the typical faulting parameters for moderate earthquakes (6.0 < Mw < 6.5) in central Apennines. For continuous ground cracks shorter than a few hundred meters and/or that show displacements smaller than 1–2 cm, the described approach may be less helpful, most probably due to the limited resolution of the data.

The review of all available contemporary documents, i.e., technical and photographic reports, newspapers, and other archive material, of the 1908, M 7.1, Messina–Reggio Calabria earthquake has allowed to recognize and classify a large number (365) of independent environmental effects of the earthquake and the subsequent tsunami. The effects have been categorized as slope movement, ground settlement, ground vertical movement and coastal retreat, liquefaction, ground crack, hydrological anomaly, gas emission, light, and rumble. No undisputable evidence of surface faulting has been pointed out. Nevertheless, the widespread and rather homogeneous subsidence of the coastal area of Messina (40–70 cm) might have been caused by slip along a NNE–SSW-trending, east-dipping fault, corresponding to the here proposed Messina Lineament. Likewise, slips along the Reggio Calabria, Armo and Motta San Giovanni faults, suggested by coastal subsidence and slides, ground cracks, and leveling data, cannot be ruled out. A large part of the achieved information has allowed to evaluate the epicentral intensity and local intensities at 74 sites, by applying the environmental seismic intensity scale ESI 2007. This study has led to the most comprehensive picture of the distribution and characteristics of coseismic environmental effects of that earthquake, a crucial knowledge to better estimate the future impact of a similar earthquake on a region that has seen a broad, and often poorly concerned, urban and infrastructural development since then.

We present a 1:25,000 scale map of the coseismic surface ruptures following the 30 October 2016 M w 6.5 Norcia normal-faulting earthquake, central Italy. Detailed rupture mapping is based on almost 11,000 oblique photographs taken from helicopter flights, that has been verified and integrated with field data (>7000 measurements). Thanks to the common efforts of the Open EMERGEO Working Group (130 people, 25 research institutions and universities from Europe), we were able to document a complex surface faulting pattern with a dominant strike of N135°-160° (SW-dipping) and a subordinate strike of N320°-345° (NE-dipping) along about 28 km of the active Mt. Vettore-Mt. Bove fault system. Geometric and kinematic characteristics of the rupture were observed and recorded along closely spaced, parallel or subparallel, overlapping or step-like synthetic and antithetic fault splays of the activated fault systems, comprising a total surface rupture length of approximately 46 km when all ruptures were considered.

SOCS3 (suppressor of cytokine signaling 3) protein is a crucial regulator of cytokine-induced inflammation, and its administration has been shown to have therapeutic effects. Recently, we designed a chimeric proteomimetic of SOCS3, mimicking the interfacing regions of a ternary complex composed of SOCS3, JAK2 (Janus kinase 2) and gp130 (glycoprotein 130) proteins. The derived chimeric peptide, KIRCONG chim, demonstrated limited mimetic function owing to its poor water solubility. We report investigations concerning a PEGylated variant of KIRCONG mimetic, named KIRCONG chim, bearing a PEG (Polyethylene glycol) moiety as a linker of noncontiguous SOCS3 regions. Its ability to bind to the catalytic domain of JAK2 was evaluated through MST (MicroScale Thermophoresis), as well as its stability in biological serum assays. The structural features of the cyclic compounds were investigated by CD (circular dichroism), nuclear magnetic resonance (NMR), and molecular dynamic (MD) studies. To evaluate the cellular effects, we employed a PLGA-nanoparticle as a delivery system after characterization using DLS and SEM techniques. KIRCONG chim PEG-revealed selective penetration into triple-negative breast cancer (TNBC) MDA-MB-231 cells with respect to the human breast epithelial cell line (MCF10A), acting as a potent inhibitor of STAT3 phosphorylation. Overall, the data indicated that miniaturization of the SOCS3 protein is a promising therapeutic approach for aberrant dysregulation of JAK/STAT during cancer progression.

Tuberculosis (TB) remains the first cause of death from infection caused by a bacterial pathogen. Chemotherapy does not eradicate (Mtb) from human lungs, and the pathogen causes a latent tuberculosis infection that cannot be prevented by the currently available Bacille Calmette Guerin (BCG) vaccine, which is ineffective in the prevention of pulmonary TB in adults. HLA-E-restricted CD8+ T lymphocytes are essential players in protective immune responses against Mtb. Hence, expanding this population or may be crucial for vaccination or immunotherapy against TB. The enzymatically inactive Bordetella pertussis adenylate cyclase (CyaA) toxoid is an effective tool for delivering peptide epitopes into the cytosol of antigen-presenting cells (APC) for presentation and stimulation of specific CD8+ T-cell responses. In this study, we have investigated the capacity of the CyaA toxoid to deliver Mtb epitopes known to bind HLA-E for the expansion of human CD8+ T cells . Our results show that the CyaA-toxoid containing five HLA-E-restricted Mtb epitopes causes significant expansion of HLA-E-restricted antigen-specific CD8+ T cells, which produce IFN-γ and exert significant cytotoxic activity towards peptide-pulsed macrophages. HLA-E represents a promising platform for the development of new vaccines; our study indicates that the CyaA construct represents a suitable delivery system of the HLA-E-binding Mtb epitopes for and expansion of HLA-E-restricted CD8+ T cells inducing a predominant Tc1 cytokine profile with a significant increase of IFN-γ production, for prophylactic and immunotherapeutic applications against Mtb.

We investigated the presence and diversity of cetaceans in the Mediterranean Sea, analysing the data collected by 32 different research units, over a period of 15 years (2004–2018), and shared on the common web-GIS platform named Intercet. We used the encounter rate, the species prevalence, and the Shannon diversity index as parameters for data analysis. The results show that cetacean diversity, in the context of the Mediterranean basin, is generally quite low when compared with the eastern Atlantic, as few species, namely the striped dolphin, the bottlenose dolphin, the fin whale, and the sperm whale, dominate over all the others. However, some areas, such as the Alboran Sea or the north-western Mediterranean Sea, which includes the Pelagos Sanctuary (the Specially Protected Area of Mediterranean Interest located in the northern portion of the western basin), show higher levels of diversity and should be considered hotspots to be preserved. Primary production and seabed profile seem to be the two main drivers influencing the presence and distribution of cetaceans, with the highest levels of diversity observed in areas characterized by high levels of primary production and high bathymetric variability and gradient. This collective work underlines the importance of data sharing to deepen our knowledge on marine fauna at the scale of the whole Mediterranean Sea and encourages greater efforts in the networking process, also to accomplish the requirements of the Marine Strategy Framework Directive, with particular reference to Descriptor 1: biological diversity is maintained.

Tuberculosis (TB) remains one of the most important health challenges worldwide. Control of the TB epidemic has not yet been achieved because of the lack of an effective vaccine and rapid and sensitive diagnostic approaches, as well as the emergence of drug-resistant forms of M. tuberculosis. Cellular immunity has a pivotal role against M. tuberculosis infection, but the role of humoral immunity is still controversial. We analyzed the frequency, absolute counts, and phenotypic and functional subsets of B lymphocytes in the peripheral blood of patients with active TB and subjects with latent infection compared to healthy donors. Moreover, we analyzed serum levels of total Ig and their IgA, IgM, and IgG isotypes and the titers of preexisting antibodies against a pool of common viral pathogens. FlowCT and unsupervised clusterization analysis show that patients with active TB and LTBI subjects have modest non-significant reduction in the numbers of circulating B lymphocytes as compared to healthy donors. Moreover, LTBI subjects had high percentages of atypical B cell population and lower percentages of naive and switched memory B cells. These findings were supported by gene expression and GSEA analysis. Moreover, there were no differences between active TB patients, LTBI subjects and HD, either in serum levels of total Ig isotypes or in preexisting IgG antibody titers, to ten different antigens from eight common pathogenic viruses, clearly demonstrating that either active or latent M. tuberculosis infection preserves the antibody production capacity of long-lived plasma cells. Thus, our results agree with previous studies reporting unaltered B cell frequencies in the blood of active TB patients and LTBI individuals as compared to healthy controls.