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Recent research shows that the meat from beef animals finished on pasture has greater concentrations of omega-3 fatty acid (FA) and conjugated linoleic acids (CLA) compared with animals finished on high-concentrate diets. However, little is known about the FA concentrations in forage that might alter these FA in the meat of pasture-finished beef. The objective was to determine the FA variation between and within forage species commonly grown in pastures in the Midwest. A secondary objective was to identify phenotypic characteristics that may be associated with individual FA. The forages analyzed included multiple cultivars of orchardgrass (Dactylis glomerata L.), tall fescue [Lolium arundinaceum (Schreb.) S.J. Darbyshire = Schedonorus phoenix (Scop.) Holub], perennial ryegrass (Lolium perenne L.), and alfalfa [Medicago sativa L. ssp. sativa and falcata (L.) Arcang.]. Grasses had higher amounts of -linolenic (C18:3) acid compared with alfalfa. Conversely, alfalfa had larger amounts of linoleic acid (C18:2) than did the grasses. Correlations between phenotypic traits and specific FA were found; plant total chlorophyll had the greatest correlation to total FA concentration. Overall, there is not a large amount of within-species variation that breeders could use to make large changes in FA concentrations.

Tall fescue [Festuca arundinacea Schreb. syn. Lolium arundinaceum (Schreb.) Darbysh.] is widely used for hay and pasture in the Eastern regions of the United States, with new types of Mediterranean tall fescue possessing superior growth under cool growing conditions. However, they do not survive Midwestern winters. To date, little research has examined the role of endophyte infection on the ability of tall fescue to grow under cold conditions or survive freezing conditions. This study's objectives determined the endophyte's role in tall fescue growth and production of metabolites for nonfreezing conditions. Additionally, the role of endophyte infection on the ability of plants to survive freezing temperatures was determined. This experiment used five genotypes of tall fescue. Half of the plants from each genotype were endophyte infected (E+), with the remaining genotypes being endophyte free (E–). We report for the first time the effects of endophyte status on proline, simple sugars, and fructan accumulation when plants were grown under cold, nonfreezing temperatures and the survivability at various freezing temperatures. Endophyte removal only affected fructan concentrations at 4.5°C; however, plant genotype affected leaf extension rate, proline, sugars, and fructan concentration. The freezing test found no effect of the endophyte on tiller survival, while plant genotype was significant. These data indicate that endophyte removal has little influence on the measured traits, and plant genotype is more critical for survival under cold conditions.