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Studying the carbon effect of land use in watersheds is important for mitigating global warming, promoting coordinated emission reduction in different regions within the watersheds, and realizing high-quality development of the watersheds. Although a number of scholars have carried out relevant studies in the past, they mainly focused on carbon emissions, rarely involved the carbon balance formed by carbon sources and sinks, and lacked relevant studies on the development of low-carbon economy sub-region. Based on this, this study takes the Yellow River Basin as an example, explores the spatial and temporal evolution of carbon emissions from land use in counties in the Yellow River Basin from 1980 to 2020, and predicts the spatial pattern of carbon income and expenditure from land use under natural conditions in 2030 and 2060 using the PLUS model; and then superimposes on the main functional area planning, divides 735 counties in the Yellow River Basin into six low-carbon economic development subregions, and analyzes their economic development The model of their economic development is analyzed. The results show that: (1) the spatial and temporal differentiation of land use carbon balance in the Yellow River Basin has changed greatly over the past 40 years, (2) the spatial distribution pattern of land use carbon balance in the natural context in 2030 and 2060 is more similar to that in 1990, (3) the carbon emission reduction potentials and pattern optimization of the different low-carbon economic development subregions differ greatly, and they have different low-carbon economic development patterns. The results of this study provide a theoretical basis for scientifically and rationally formulating economic policies for low-carbon development in the counties of the Yellow River Basin, and also provide an important reference for related studies in other similar basins or regions in the world.

Notoginseng ( Panax notoginseng ), a valuable herbal medicine, has high death rates in continuous cropping systems. Variation in the soil microbial community is considered the primary cause of notoginseng mortality, although the taxa responsible for crop failure remains unidentified. This study used high-throughput sequencing methods to characterize changes in the microbial community and screen microbial taxa related to the death rate. Fungal diversity significantly decreased in soils cropped with notoginseng for three years. The death rate and the fungal diversity were significantly negatively correlated, suggesting that fungal diversity might be a potential bioindicator of soil health. Positive correlation coefficients revealed that Burkholderiales, Syntrophobacteraceae, Myrmecridium , Phaeosphaeria , Fusarium and Phoma were better adapted to colonization of diseased plants. The relative abundance of Fusarium oxysporum ( R  = 0.841, P  < 0.05) and Phaeosphaeria rousseliana ( R  = 0.830, P  < 0.05) were positively associated with the death rate. F. oxysporum was a pathogen of notoginseng root-rot that caused seedling death. Negative correlation coefficients indicated that Thermogemmatisporaceae, Actinosynnemataceae, Hydnodontaceae, Herpotrichiellaceae and Coniosporium might be antagonists of pathogens and the relative abundance of Coniosporium perforans was negatively correlated with the death rate. Our findings provide a dynamic overview of the microbial community and present a clear scope for screening beneficial microbes and pathogens of notoginseng.

Background Ganoderma lucidum , a well-known medicinal fungus in traditional Chinese medicine, exhibits a superior growth rate in its dikaryotic mycelia compared to monokaryons, and this is essential for its fruiting body formation and medicinal utilization. However, the biological process landscape contributing to the dikaryon growth advantage remains largely unexplored. This study performed Tandem Mass Tag (TMT)-based proteomic analysis of the dikaryotic mycelia and two compatible monokaryotic mycelia to elucidate the mechanism underlying this growth advantage. Results Dikaryotic mycelia showed superior growth rates over monokaryons in both solid and liquid cultures. Proteomic analysis revealed a remodeled proteome of the dikaryon, with elevated expression levels of proteins involved in biosynthesis and processing of protein and RNA, cell structure, and cell division, compared to monokaryons, in both global proteins (3990) and differentially expressed proteins (983) analysis. In contrast, metabolism related proteins, particularly those involved in energy production and conversion, expressed at lower levels in dikaryon compared to monokaryons. Nonetheless, the energy-consuming proteins showed high expression in dikaryon. Conclusions These results indicate diverse remodeled biological processes coordinately contribute to the dikaryon growth advantage, especially the transfer from energy metabolism to biosynthesis and growth with high energy utilization efficiency. This is the first discovery of metabolic remodeling in dikaryotic mycelia of G. lucidum , presenting favorable implications for the breeding of G. lucidum .

Heavy metal contamination in herbal medicines is a global threat to human beings especially at levels above known threshold concentrations. The concentrations of five heavy metals cadmium (Cd), lead (Pb), arsenic (As), mercury (Hg) and copper (Cu) were investigated using Inductively Coupled Plasma Optical Mass Spectrometry (ICP-MS) with 1773 samples around the world. According to Chinese Pharmacopoeia, 30.51% (541) samples were detected with at least one over-limit metal. The over-limit ratio for Pb was 5.75% (102), Cd at 4.96% (88), As at 4.17% (74), Hg at 3.78% (67), and of Cu, 1.75% (31). For exposure assessment, Pb, Cd, As, and Hg have resulted in higher than acceptable risks in 25 kinds of herbs. The maximal Estimated Daily Intake of Pb in seven herbs, of Cd in five, of Hg in four, and As in three exceeded their corresponding Provisional Tolerable Daily Intakes. In total 25 kinds of herbs present an unacceptable risk as assessed with the Hazard Quotient or Hazard Index. Additionally, the carcinogenic risks were all under acceptable limits. Notably, As posed the highest risk in all indicators including Estimated Daily Intake, Hazard Index, and carcinogenic risks. Therefore further study on enrichment effect of different states of As and special attention to monitoring shall be placed on As related contamination.

Sophora flavescens are widely used for their pharmacological effects. As its main pharmacological components, alkaloids and flavonoids are distributed in the root tissues wherein molecular mechanisms remain elusive. In this study, metabolite profiles are analyzed using metabolomes to obtain biomarkers detected in different root tissues. These biomarkers include alkaloids, phenylpropanoids, and flavonoids. The high-performance liquid chromatography analysis results indicate the differences in principal component contents. Oxymatrine, sophoridine, and matrine contents are the highest in the phloem, whereas trifolirhizin, maackiain, and kushenol I contents are the highest in the xylem. The transcript expression profiles also show tissue specificity in the roots. A total of 52 and 39 transcripts involved in alkaloid and flavonoid syntheses are found, respectively. Among them, the expression levels of LYSA1, LYSA2, AO2, AO6, PMT1, PMT17, PMT34, and PMT35 transcripts are highly and positively correlated with alkaloids contents. The expression levels of 4CL1, 4CL3, 4CL12, CHI5, CHI7, and CHI9 transcripts are markedly and positively correlated with flavonoids contents. Moreover, the quantitative profiles of alkaloids and flavonoids are provided, and the pivotal genes regulating their distribution in S. flavescens are determined . These results contribute to the existing data for the genetic improvement and target breeding of S. flavescens .

Hydroxypyridinones (HOPOs) have been used in the chelation therapy of iron and actinide metals. Their application in metal-based radiopharmaceuticals has also been increasing in recent years. This review article focuses on how multidentate HOPOs can be used in targeted radiometal-based diagnostic and therapeutic radiopharmaceuticals. The general structure of radiometal-based targeted radiopharmaceuticals, a brief description of siderophores, the basic structure and properties of bidentate HOPO, some representative HOPO multidentate chelating agents, radiopharmaceuticals based on HOPO multidentate bifunctional chelators for gallium-68, thorium-227 and zirconium-89, as well as the future prospects of HOPO multidentate bifunctional chelators in other metal-based radiopharmaceuticals are described and discussed in turn. The HOPO metal-based radiopharmaceuticals that have shown good prospects in clinical and preclinical studies are gallium-68, thorium-227 and zirconium-89 radiopharmaceuticals. We expect HOPO multidentate bifunctional chelators to be a very promising platform for building novel targeted radiometal-based diagnostic and therapeutic radiopharmaceuticals.

Background The programmed cell death-1/programmed cell death-ligand 1 (PD-1/PD-L1) pathway has been shown to be involved in trauma-induced immunosuppression and to influence CD4 + T cell differentiation. MicroRNA (miR)-21 is a critical player in immune responses. However, it remains largely unknown whether miR-21 is regulated by PD-1 and influences CD4 + T-cell lineage choice after gastric cancer resection. Methods In the present study, we analyzed the percentages of T-helper (Th)-17/regulatory T (Treg) cells and PD-1/PD-L1 expression on peripheral blood mononuclear cells (PBMCs) during the perioperative period. We also detected the secretion of interleukin (IL)-17 and transforming growth factor (TGF)-β1 using enzyme-linked immunosorbent assays (ELISAs). Furthermore, PBMCs isolated from patients were transfected with or without adenovirus-short hairpin-PD-1 (Ad-sh-PD1), pre-miR-21 or adenovirus–green fluorescent protein (Ad-GFP), and the percentages of Th17/Treg cells and related transcription factors were measured. Results In patients who underwent gastric cancer resection, the number of Th17 cells decreased, whereas the number of Treg cells increased, accompanied by an increased expression of PD-1/PD-L1. In addition, the expression of RORγt and IL-17 decreased, whereas the expression of Foxp3 and TGF-β1 increased. In vitro, silencing PD-1 via Ad-sh-PD1 promoted the expression of miR-21 and increased the percentage of Th17 cells, but decreased the percentage of Treg cells. The overexpression of miR-21 increased the percentage of Th17 cells but decreased the percentage of Treg cells. Conclusions Our study demonstrated that gastric cancer resection altered the balance of Th17/Treg cells and increased PD-1/PD-L1 expression. In the in vitro experiments, the transfection of Ad-sh-PD1 ameliorated Th17/Treg cell imbalance partially by increasing the expression of miR-21.

Secretory proteins are cotranslationally or posttranslationally translocated across lipid membranes via a protein-conducting channel named SecY in prokaryotes and Sec61 in eukaryotes. The vast majority of secretory proteins in bacteria are driven through the channel posttranslationally by SecA, a highly conserved ATPase. How a polypeptide chain is moved by SecA through the SecY channel is poorly understood. Here, we report electron cryomicroscopy structures of the active SecA–SecY translocon with a polypeptide substrate. The substrate is captured in different translocation states when clamped by SecA with different nucleotides. Upon binding of an ATP analog, SecA undergoes global conformational changes to push the polypeptide substrate toward the channel in a way similar to how the RecA-like helicases translocate their nucleic acid substrates. The movements of the polypeptide substrates in the SecA–SecY translocon share a similar structural basis to those in the ribosome–SecY complex during cotranslational translocation.

Several kinds of cancer surgeries with propofol-based total intravenous anesthesia (TIVA) have been shown to have better outcomes than those with sevoflurane-based inhalational anesthesia (INHA). However, the effects of this anesthetic technique have not been investigated in patients with gastric cancer. In this study, the authors retrospectively examined the link between the choice of anesthetic technique and overall survival in patients undergoing gastric cancer resection. We conducted a retrospective analysis of the database of all patients undergoing gastric cancer resection for gastric cancer between 2007 and 2012. Patients who received TIVA or INHA were administered patient-controlled intravenous analgesia for 72-120 hours postoperatively. Survival was estimated using the Kaplan-Meier log-rank test, and associations between anesthetic technique and outcomes were analyzed using Cox proportional hazards regressions after propensity matching. A total of 2,856 anesthetics using INHA or TIVA were delivered in the study period. After propensity matching, 897 patients remained in each group. According to Kaplan-Meier analysis, the use of TIVA was associated with improved survival ( <0.001). TIVA was associated with a hazard ratio (HR) of 0.67 (95% confidence interval [CI]: 0.58-0.77) for death in univariate analysis and 0.65 (95% CI: 0.56-0.75) after a multivariate analysis of known confounders in the matched group. Cancer stage (HR =0.74, 95% CI: 0.64-0.86, <0.001) and degree of differentiation (HR =1.28, 95% CI: 1.11-1.47, <0.001) were also associated with survival in the univariate analysis in the matched group. In the multivariable Cox model, cancer stage (HR =0.72, 95% CI: 0.62-0.84, <0.001) and degree of differentiation (HR =1.23, 95% CI: 1.07-1.42, <0.001) were associated with survival in the matched group. These results indicate that TIVA may be associated with improved survival in gastric cancer patients who undergo resection.

Lauric acid is a bioactive root exudate component in crown daisy. Mi-flp-18 is a pivotal gene regualting nematode chemotaxis and infection. Lauric acid regulates the nematode chemotaxis and disrupts the Mi-flp-18 expression in a concentration-dependent manner