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Black soybeans have a long history in China, with rich nutrition and efficacy. The extraction of its active ingredients is mostly focused on methods such as ultrasound, microwave, Soxhlet extraction, and distillation. These methods rely heavily on solvents, which are not environmentally friendly and healthy, and limit the development and utilization of their functional compositions. This study utilized supercritical fluid extraction technology to prepare functional components of black soybeans and optimized the supercritical CO 2 fluid extraction process of black soybean components through response surface methodology. The qualitative and quantitative analyses of fatty acids in black soybean extracts were mainly conducted, and the free radical scavenging ability of the extracts was studied. The results showed that the black soybean compositions extracted by supercritical fluid were rich, mainly including unsaturated fatty acids and terpenes and had a certain scavenging ability for DPPH and hydroxyl radicals. This study provides a theoretical reference for the industrial preparation of black soybean extractions and also provides ideas for the preparation of functional compositions of other medicinal and edible homologous substances.

Black soybeans have a long history in China, with rich nutrition and efficacy. The extraction of its active ingredients is mostly focused on methods such as ultrasound, microwave, Soxhlet extraction, and distillation. These methods rely heavily on solvents, which are not environmentally friendly and healthy, and limit the development and utilization of their functional compositions. This study utilized supercritical fluid extraction technology to prepare functional components of black soybeans and optimized the supercritical CO[sub.2] fluid extraction process of black soybean components through response surface methodology. The qualitative and quantitative analyses of fatty acids in black soybean extracts were mainly conducted, and the free radical scavenging ability of the extracts was studied. The results showed that the black soybean compositions extracted by supercritical fluid were rich, mainly including unsaturated fatty acids and terpenes and had a certain scavenging ability for DPPH and hydroxyl radicals. This study provides a theoretical reference for the industrial preparation of black soybean extractions and also provides ideas for the preparation of functional compositions of other medicinal and edible homologous substances.

Minor-adjustment of the retention of peptides, induced by varying the mobile phase flow-rate（MPF-R）, is a new dynamic separation method for simultaneously and rapidly identifying and improving the selectivity of hidden and overlapping peptide peaks. It can also-stabilize the reverse elution order of some pair-peaks under gradient elution in reverse phase liquid chromatography. The retention characteristics of peptides under gradient elution in RPLC was firstly found to be dominated by two variables of the steady region（SR） and migration region（MR）. The changes in peptide retention induced by varying the MPF-R can be attributed to changes in the rate of bond breaking of multiple molecular interactions of peptides from the SR and of the mass transfer of peptides from the stationary phase to the mobile phase in the MR. The two dynamic variables were also found to independently depend on the type of peptide. Desirable results were obtained using six standard oligopeptides and a real sample of trypsin-digested lysozyme.It is expected that the quality control of peptide drugs, high dispersion of peptide peaks in peptide mapping and ＂bottom-up MS＂in proteomics will be improved by this method, even enabling peptide purification on a preparative scale in industry.

Background：Core fucosylation （CF）,catalyzed by α-1,6 fucosyltransferase （Fut8） in mammals,plays an important role in pathological processes through posttranslational modification of key signaling receptor proteins,including transforming growth factor （TGF）-β receptors and platelet-derived growth factor （PDGF） receptors.However,its effect on peritoneal fibrosis is unknown.Here,we investigated its influence on epithelial-mesenchymal transition （EMT） of rat peritoneal mesothelial cells （PMCs） in vitro induced by a high-glucose （HG） culture solution.Methods：Rat PMCs were first cultured in a HG （2.5%） culture solution to observe the CF expression level （fluorescein isothiocyanate-lens culinaris agglutinin）,we next established a knockdown model of rat PMCs in vitro with Fut8 small interfering RNA （siRNA） to observe whether inhibiting CF decreases the messenger RNA （mRNA） expression and protein expression of Fut8 and reverses EMT status.Rat PMCs were randomly divided into control group,mock group （transfected with scrambled siRNA）,Fut8 siRNA group,HG group,HG ＋ mock group,and HG ＋ Fut8 siRNA group.Finally,we examined the activation of TGF-β/Smad2/3 signaling and PDGF/extracellular signal-regulated kinase （ERK） signaling to observe the influence of CF on them.Results：CF,Fut8 mRNA,and protein expression were all significantly upregulated in HG-induced EMT model than those in the control rat PMCs （P 〈 0.05）.Fut8 siRNA successfully blocked CF of TGF-β receptors and PDGF receptors and attenuated the EMT status （E-cadherin and α-SMA and phenotypic changes） in HG-induced rat PMCs.In TGF-β/Smad2/3 signaling,Fut8 siRNA did not suppress the protein expression of TGF-3 receptors and Smad2/3;however,it significantly suppressed the phosphowlation of Smad2/3 （relative expression folds of HG ＋ Fut8 group vs.HG group：7.6 ± 0.4 vs.15.1 ± 0.6,respectively,P 〈 0.05）.In PDGF/ERK signaling,Fut8 siRNA did not suppress the protein expression of PDGF receptors and ERK,but it significantly suppressed the phosphorylation of ERK （relative expression folds of HG ＋ Fut8 group vs.HG group：8.7 ± 0.9 vs.15.6 ± 1.2,respectively,P 〈 0.05）.Blocking CF inactivated the activities of TGF-β and PDGF signaling pathways,and subsequently blocked EMT.Conclusions：These results demonstrate that CF contributes to rat PMC EMT.and that blocking it attenuates EMT.CF regulation is a potential therapeutic target of peritoneal fibrosis.

Heavy metals (HMs) normally occur in nature and are rapidly released into ecosystems by anthropogenic activities, leading to a series of threats to plant productivity as well as human health. Phytoremediation is a clean, eco-friendly, and cost-effective method for reducing soil toxicity, particularly in weedy plants (invasive plant species (IPS)). This method provides a favorable tool for HM hyperaccumulation using invasive plants. Improving the phytoremediation strategy requires a profound knowledge of HM uptake and translocation as well as the development of resistance or tolerance to HMs. This review describes a comprehensive mechanism of uptake and translocation of HMs and their subsequent detoxification with the IPS via phytoremediation. Additionally, the improvement of phytoremediation through advanced biotechnological strategies, including genetic engineering, nanoparticles, microorganisms, CRISPR-Cas9, and protein basis, is discussed. In summary, this appraisal will provide a new platform for the uptake, translocation, and detoxification of HMs via the phytoremediation process of the IPS.

Multi-view inverse synthetic aperture radar (ISAR) image sequences provide multi-dimensional observation information about space targets, enabling precise attitude estimation that is fundamental to both non-cooperative target monitoring and critical space operations including active debris removal and space collision avoidance. However, directly utilizing all images within an ISAR sequence for attitude estimation can result in a substantial data preprocessing workload and reduced algorithm efficiency. Given the inherent overlap and redundancy in the target information provided by these ISAR images, this paper proposes a novel space target attitude estimation method based on the selection of multi-view ISAR image sequences. The proposed method begins by establishing an ISAR imaging projection model, then characterizing the target information differences through variations in imaging plane normal, and proposing an image selection method based on the uniform sampling across elevation and azimuth angles of the imaging plane normal. On this basis, the method utilizes a high-resolution network (HRNet) to extract the feature points of typical components of the space target. This method enables simultaneous feature point extraction and matching association within ISAR images. The attitude estimation problem is subsequently modeled as an unconstrained optimization problem. Finally, the particle swarm optimization (PSO) algorithm is employed to solve this optimization problem, thereby achieving accurate attitude estimation of the space target. Experimental results demonstrate that the proposed methodology effectively filters image data, significantly reducing the number of images required while maintaining high attitude estimation accuracy. The method provides a more informative sequence than conventional selection strategies, and the tailored HRNet + PSO estimator resists performance degradation in sparse-data conditions, thereby ensuring robust overall performance.

Background NAFLD is tightly associated with various diseases such as diabetes, cardiovascular disease, kidney disease, and cancer. Previous studies had investigated the association between NAFLD and various extrahepatic cancers, but the available data to date is not conclusive. The aim of this study was to investigate the association between NAFLD and various extrahepatic cancers comprehensively. Methods Searches were conducted of various electronic databases (PubMed, EMBASE, Medline, and the Cochrane Library) to identify observational studies published between 1996 and January 2020 which investigated the association between NAFLD and extrahepatic cancers. The pooled OR/HR/IRR of the association between NAFLD and various extrahepatic cancers were analyzed. Results A total of 26 studies were included to investigate the association between NAFLD and various extrahepatic cancers. As the results shown, the pooled OR values of the risk of colorectal cancer and adenomas in patients with NAFLD were 1.72 (95%CI: 1.40–2.11) and 1.37 (95%CI: 1.29–1.46), respectively. The pooled OR values of the risk of intrahepatic cholangiocarcinoma and extrahepatic cholangiocarcinoma in patients with NAFLD were 2.46 (95%CI: 1.77–3.44) and 2.24 (95%CI: 1.58–3.17), respectively. The pooled OR value of the risk of breast cancer in patients with NAFLD was 1.69 (95%CI: 1.44–1.99). In addition, NAFLD was also tightly associatied with the risk of gastric cancer, pancreatic cancer, prostate cancer, and esophageal cancer. Conclusions NAFLD could significantly increase the development risk of colorectal adenomas and cancer, intrahepatic and extrahepatic cholangiocarcinoma, breast, gastric, pancreatic, prostate, and esophageal cancer. NAFLD could be considered as one of the influencing factors during the clinical diagnosis and treatment for the extrahepatic cancers.

Background A growing number of studies reported the connection between the level of serum ferritin (SFL) and non-alcoholic fatty liver disease (NAFLD). However, such connection was still disputable. The aim of our meta-analysis was to estimate SFL between the groups as below: patients with NAFLD against control group; non-alcoholic steatohepatitis (NASH) patients against control group; non-alcoholic fatty liver (NAFL) patients against a control group and NASH patients vs NAFL patients. Methods We screened the studies in PubMed, EMBASE, the Cochrane Database and the Cochrane Central register controlled trials from the beginning to July 10, 2016 to find the studies indicated the connection between SFL and NAFLD (NAFL and/or NASH). Fourteen published studies which evaluate the SFL in NAFLD patients were selected. Results Higher SFL was noticed in NAFLD patients against control group (standardized mean difference [SMD] 1.01; 95% CI 0.89, 1.13), NASH patients against control group (SMD 1.21; 95% CI 1.00, 1.42), NAFL patients against control group (SMD 0.51; 95% CI 0.24, 0.79) and NASH patients against NAFL patients (SMD 0.63; 95% CI 0.52, 0.75). These results remained unaltered actually after the elimination of studies which were focused on paediatric or adolescent populations. Higher SFL was presented in NAFLD patients against the control group (SMD 1.08; 95% CI 0.95, 1.20) in adults and NASH patients against NAFL patients in adults (SMD 0.74; 95% CI 0.62, 0.87). The connection between SFL and NASH against NAFL group in paediatric or adolescent populations was observed inconsistently (SMD 0.10; 95% CI -0.18, 0.38). Conclusions The level of SFL was elevated in patients with NAFLD (NAFL and/or NASH) compared with the controls. Compared with NAFL, The level of SFL was increased in NASH. The result remained unaltered actually after the elimination of studies focused on paediatric or adolescent populations.

Background Present evidences suggested that TRIB1 rs17321515 polymorphism was tightly associated with the increased risk of NAFLD and CHD. CHD is one of the main complications of NAFLD, whether TRIB1 rs17321515 polymorphism could affect the risk of CHD in general population and NAFLD patients in Chinese Han population was remain unknown. The present study was designed to investigate the association between TRIB1 rs17321515 polymorphism and the risk of CHD in general population and NAFLD patients in Chinese Han population, and investigate the effect of TRIB1 rs17321515 polymorphism on serum lipid levels. Patients and methods TRIB1 rs17321515 gene polymorphism was genotyped using the polymerase chain reaction (PCR) in healthy controls ( n  = 175), CHD patients ( n  = 155), NAFLD patients ( n  = 146), and NAFLD+CHD patients ( n  = 156). Serum lipid profiles were determined using biochemical methods. Statistical analyses were performed using SPSS 24.0 statistical software. Results The TRIB1 rs17321515 AA+GA genotypes were the significant risk factors for the CHD in general population (OR = 1.788; 95% CI: 1.104–2.897; P  = 0.018) and in the NAFLD patients (OR = 1.760; 95% CI: 1.071–2.891; P  = 0.026). After adjusted for age, gender, and body mass index, the risk for CHD in general population (OR = 1.857; 95% CI: 1.116–3.089; P  = 0.017) and NAFLD patients was still significant (OR = 1.723; 95% CI: 1.033–2.873; P  = 0.037). In addition, TRIB1 rs17321515 A carriers possess the higher lipid profiles in the included subjects. Conclusions TRIB1 rs17321515 AA+GA genotypes were significant associated with the risk of CHD in general population and in NAFLD patients in Chinese Han population. The rs17321515 A allele increases the serum lipid profiles in included subjects.

Invasive alien species are a growing threat to natural systems, the economy, and human health. Active surveillance and responses that readily suppress newly established colonies are effective actions to mitigate the noxious consequences of biological invasions. 'Aedes (Hulecoeteomyia) koreicus (Edwards)', a mosquito species native to East Asia, has spread to parts of Europe and Central Asia since 2008. In the last decade, 'Ae. koreicus' has been shown to be a competent vector for chikungunya virus and Dirofilaria immitis. However, information about the current and potential distribution of 'Ae. koreicus' is limited. Therefore, to understand the changes in their global distribution and to contribute to the monitoring and control of 'Ae. koreicus', in this study, the MaxEnt model was used to predict and analyze the current suitable distribution area of 'Ae. koreicus' in the world to provide effective information.