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Whipworms ( Trichuris spp) are ubiquitous parasites of humans and domestic and wild mammals that cause chronic disease, considerably impacting human and animal health. Egg hatching is a critical phase in the whipworm life cycle that marks the initiation of infection, with newly hatched larvae rapidly migrating to and invading host intestinal epithelial cells. Hatching is triggered by the host microbiota; however, the physical and chemical interactions between bacteria and whipworm eggs, as well as the bacterial and larval responses that result in the disintegration of the polar plug and larval eclosion, are not completely understood. Here, we examined hatching in the murine whipworm, Trichuris muris , and investigated the role of specific bacterial and larval structures and molecules in this process. Using scanning and transmission electron microscopy, we characterised the physical interactions of both fimbriated ( Escherichia coli , Salmonella typhimurium and Pseudomonas aeruginosa ) and non-fimbriated ( Staphylococcus aureus) bacteria with the egg polar plugs during the induction/initiation stage, and visualised the effects of structural changes in the polar plugs, leading to larval eclosion. Further, we found that protease inhibitors blocked whipworm hatching induced by both fimbriated and non-fimbriated bacteria in a dose-dependent manner, suggesting the partial involvement of bacterial enzymes in this process. In addition, we identified the minimal egg developmental timing required for whipworm hatching, and transcriptomic analysis of T . muris eggs through embryonation revealed the specific upregulation of serine proteases (S01A family) in fully embryonated eggs containing ‘hatch-ready’ L1 larvae. Finally, we demonstrated that inhibition of serine proteases with the serine-protease inhibitor Pefabloc ablated T . muris egg hatching induced by bacteria. Collectively, our findings unravel the temporal and physicochemical bacterial-egg interactions leading to whipworm hatching and indicate serine proteases of both bacterial and larval origin mediate these processes.

In Colombia, the cutaneous leishmaniasis (CL) is the most common manifestation across the army personnel. Hence, it is mandatory to determine the species associated with the disease as well as the association with the clinical traits. A total of 273 samples of male patients with CL were included in the study and clinical data of the patients was studied. PCR and sequencing analyses (Cytb and HSP70 genes) were performed to identify the species and the intra-specific genetic variability. A georeferenced database was constructed to identify the spatial distribution of Leishmania species isolated. The identification of five species of Leishmania that circulate in the areas where army personnel are deployed is described. Predominant infecting Leishmania species corresponds to L. braziliensis (61.1%), followed by Leishmania panamensis (33.5%), with a high distribution of both species at geographical and municipal level. The species L. guyanensis, L. mexicana and L. lainsoni were also detected at lower frequency. We also showed the identification of different genotypes within L. braziliensis and L. panamensis. In conclusion, we identified the Leishmania species circulating in the areas where Colombian army personnel are deployed, as well as the high intra-specific genetic variability of L. braziliensis and L. panamensis and how these genotypes are distributed at the geographic level.

Grounded in an ecodevelopment perspective, in the current study we examined unique and moderating effects of daily COVID-19 prevalence (social contexts) on effects of COVID-19 related risk and protective factors such as emotional distress (individual contexts) and employment (working from home and unemployment status; family contexts) on family functioning among 160 recent immigrant families in Israel. In general, results indicate several unique effects of COVID-19 related factors (such as COVID-19 emotional distress, unemployment, and remote work arrangements) on both parents’ and adolescents’ reports of family functioning. However, results indicated that there were more significant associations between COVID-19 factors (e.g., emotional distress and COVID-19 prevalence) and family functioning indicators with adolescents, than with parents. The effects of COVID-19 factors (e.g., emotional distress and remote work arrangements) were moderated by daily COVID-19 prevalence (new cases and deaths). We discuss ways in which interventionists can contribute to pandemic-related research to promote optimal family functioning among immigrant families.

An often-stated ecomorphological assumption that has the status of ‘textbook knowledge’ is that the dimensions of the digestive tract correlate with diet, where herbivores—consuming diets of lower digestibility—have longer intestinal tracts than faunivores—consuming diets of higher digestibility. However, statistical approaches have so far failed to demonstrate this link. Here, we collated data on the length of intestinal sections and body mass of 519 mammal species, and test for various relationships with trophic, climatic and other biological characteristics. All models showed a strong phylogenetic signal. Scaling relationships with body mass showed positive allometry at exponents greater than 0.33, except for the caecum, which is particularly large in smaller species. Body mass was more tightly linked to small intestine than to large intestine length. Adding a diet proxy to the relationships increased model fit for all intestinal sections, except for the small intestine when accounting for phylogeny. Thus, the diet has a main effect on the components of the large intestine, with longer measures in herbivores. Additionally, measures of habitat aridity had a positive relationship with large intestine length. The small intestine was longer in species from colder habitats at higher latitudes, possibly facilitating the processing of peak intake rates during the growing season. This study corroborates intuitive expectations on digestive tract anatomy, while the dependence of significant results on large sample sizes and inclusion of specific taxonomic groups indicates that the relationships cannot be considered fixed biological laws.

Controlling for factors such as criminal violence and poverty, we tested if drier than usual growing season weather was a predictor of emigration from El Salvador, Guatemala, and Honduras to the US between 2012 and 2018. We focus on growing season weather because agriculture is a primary transmission pathway from the effects of climate change upon migration. We secured the migration apprehensions data for our analysis through a FOIA request to US Customs and Border Protection. Border Patrol intake interviews recorded the original home location of families that arrived at the southern US border. We used this geographic information to measure recent weather patterns and social circumstances in the area that each family departed. We found 70.7% more emigration to the US when local growing seasons in Central America were recently drier than the historical average since 1901.

The National Army of Colombia is present in all of the national territory, focused in sylvatic zones where they are exposed continually to potential risk of transmission of Trypanosoma cruzi, the etiological agent of the Chagas disease. People of this study were active personal that were born and lived during their first years in endemic areas of transmission through domiciled vectors as Rhodnius prolixus. The main aim of this study was to estimate the prevalence of Chagas disease in the active military population of the National Army, gathered in five departments. An observational and descriptive study with cross-sectional analysis was carried out. Blood sample each patient in order to apply serological diagnosis by two different Enzyme Linked ImmunoSorbent Assay tests, following the algorithm of National Institute of Health, Colombia. In cases of serum results with inconsistencies, a Hemagglutination Inhibition test and Indirect Immunofluorescence assay test were performed to solve inconclusiveness. Positive samples by two different tests were considered seropositive. Additionally, to each positive sample by at least one serological test, we did extraction of DNA for molecular diagnosis. 295 serums were analyzed and two of them were positive in order to get a prevalence of 0.68%. Two samples analyzed by molecular diagnosis were negative. The prevalence was < 1% It is probable the infection in the seropositive individuals occurred before enlisting in the military service due to origin locations with transmission such as Casanare and Boyacá. These findings allow defining the prevention and control programs of chronic cases and reduction in the disease burden.

Semiconductor lasers are very sensitive to optical feedback. Here we study experimentally the effect of optical feedback on the spatial coherence of a diode laser using the speckle technique. Speckle is a noisy structure arising from the interference of coherent waves as they propagate through a diffusive medium. Using a multimode fibre as the diffusive medium, we observe that, during the laser turn-on, without feedback, the speckle contrast increases gradually (revealing a gradual increase in spatial coherence), but, with sufficiently strong feedback, the speckle contrast increases sharply (revealing an abrupt increase in spatial coherence). For pump currents above the threshold, high-contrast regions alternate with low-contrast regions. We also observe that, under appropriate current modulation, high-contrast regions are suppressed. Our findings may find application in laser-based illumination systems, because optical feedback can be used in combination with current modulation to reduce speckles over a wide range of pump currents.

Vascularized composite allotransplantation (VCA) represents a clinical challenge for transplant therapy, as it involves different tissues with unique immunogenicity. Even when receiving immunosuppressive therapy, they are more vulnerable to severe hypoxia, microvascular damage, and ultimately the rejection or chronic graft dysfunction after transplantation. This study aimed to develop a surgical protocol for VCA of the ear in a porcine biomodel in the absence of immunosuppression, maintaining the in vitro co-culture of the allograft and assessing their relationship with allograft survival. We employed four crossbred pigs and four outbred mini pig biomodels ( Sus scrofa ), as donors and recipients, to perform four VCAs. Blood samples were taken from each biomodel for crossmatch testing and SLA haplotype identification. Bone marrow samples were taken from each recipient for subsequent co-culture. In vitro culture and co-culture conditions were maintained and assessed. Histological analysis using hematoxylin and eosin staining was performed on the allograft that lasted the longest time showing the smallest macroscopic signs of rejection. A surgical protocol for Vascularized Composite Allograft (VCA) ear transplantation in a porcine biomodel was developed, including the skin. The presence of SLA-DRB1*01:02 and SLA-DRB1*06:01 haplotypes in the recipient and donor, respectively, showed concordance with positive crossmatch tests. In the allograft with the highest survival time, no histological signs of hyperacute rejection were found ten days after transplantation in the anastomosis area. The results obtained from this protocol can provide valuable recommendations for translational applications in face transplantation and regenerative medicine.

In general, flow measurement systems in production units only report the daily total production rates. As there is no precise control of individual production of each well, the current well flow rates and their parameters are determined when production tests are conducted. Because production tests are performed periodically (e.g., once a month), information about the wells is limited and operational decisions are made using data that are not updated. Meanwhile, well properties and parameters from the production test are typically used in multiphase flow models to forecast the expected production. However, this is done deterministically without considering the different sources of uncertainties in the production tests. This study aims to introduce uncertainties in oil flow rate forecast. To do this, it is necessary to identify and quantify uncertainties from the data obtained in the production tests, consider them in production modeling, and propagate them by using multiphase flow simulation. This study comprises two main areas: data analytics and multiphase flow simulation. In data analytics, an algorithm is developed using R to analyze and treat the data from production tests. The most significant stochastic variables are identified and data deviation is adjusted to probability distributions with their respective parameters. Random values of the selected variables are then generated using Monte Carlo and Latin Hypercube Sampling (LHS) methods. In multiphase flow simulation, these possible values are used as input. By nodal analysis, the simulator output is a set of oil flow rate values, with their interval of occurrence probabilities. The methodology is applied, using a representative Brazilian offshore field as a case study. The results show the significance of the inclusion of uncertainties to achieve greater accuracy in the multiphase flow analysis of oil production.

Background Leishmaniasis is one of the most important infectious diseases affecting the Colombian National Army due to the high number of reported cases and exposure throughout military operations in endemic areas. The main aim of this study was to estimate the geographical distribution along with the genetic diversity and treatment outcome of Leishmania species in Colombian military personnel. Methods Skin lesion samples by smear and aspirate were collected in 136 patients having parasitological cutaneous leishmaniasis (CL) diagnosis. DNA was extracted, the nuclear marker heat shock protein 70 ( HSP70 ) was amplified by PCR and sequenced. Leishmania species were identified by BLASTn. The geo-spatial distribution of the identified parasites was determined according to the possible site of infection. Gene tree was constructed by maximum likelihood (ML), diversity indices ( π, h ) were estimated and haplotype network was constructed under the Templeton-Crandall-Sing algorithm in order to determine the geographic relationships of the genetic variants of Leishmania species circulating in Colombian military population. Results The species were identified in 77.94% of the samples, with a predominance of L. braziliensis (65.09%), followed by L. panamensis (31.13%), L. naiffi by the first time reported in Colombia in two patients (1.89%) as well as L. lindenbergi in a single patient (0.945%) with possible infection in the municipality of Miraflores, Guaviare and L. infantum in a single patient (0.945%) notified with CL in the municipality of Tumaco, Nariño. The phylogenetic analysis was consistent according to bootstrap, showing four strongly differentiated clades. Conclusions The geo-spatial distribution suggested that L. braziliensis has a greater abundance, while L. panamensis has a greater dispersion. The phylogenetic relationships of Leishmania species in Colombian military personnel was estimated with the confirmation of two new species circulating without prior report in the country and a species with no background for CL in the Colombian army. A substantial genetic diversity of Leishmania braziliensis was defined. This study contributes through the understanding of the molecular epidemiology to the CL transmission in Colombia.