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Background To investigate medicinal plants used in the traditional treatment of cutaneous leishmaniasis using multivariate tests coupled with pharmacokinetic predictions in silico. Main body Interviews were conducted with 21 herbalists in Annaba and El Tarf provinces. The dataset comprised 26 variables, and multidimensional analyses were applied using the FactoMineR package. Nineteen species were reported in this survey. Among them, the most important family was Lamiaceae, with five species. According to the RFC index, the most famous species were the aerial parts of Teucrium polium L. (57.14%). Second, a data matrix for the species generated showed higher contribution values in the first dimension owing to the lack of the topical administration form, the presence of preparation mode by decoction and cataplasm, and the association of medicinal plants specifically with olive oil and honey, respectively. The analysis of the first dimension demonstrated a high association for the form of administration and the mode of administration, with correlation ratios η 2 of 6.261E-01, 5.501E-01, and 4.772E-01 ( p  < 0.05), respectively. The cluster analysis identified three clusters differentiated by the administration form per os with a p-value of 7.49E-05 ( p  < 0.05). Later, the ADME profile was tested in silico by identifying the physicochemical properties of the major compounds reported in the literature. Conclusions The results showed that the major compounds of plants with high RFC showed excellent ADME profiles, with good absorption, low toxicity, and favorable distribution, making them promising candidates for the safe treatment of leishmaniasis.

Over the past decade, the therapeutic effects of essential oils have become a research interest. This study describes the chemical composition, antimicrobial, antioxidant, and insecticidal properties of Juniperus procera and Cinnamomum camphora essential oils. Essential oils were extracted by the steam distillation method and identified by gas chromatography-mass spectrometry (GC-MS), revealing the presence of monoterpenes and sesquiterpenes as the main components of J. procera, together with α-pinene, endo-Borneol, and 1, 8-cineole. C. camphora showed high concentrations of α-pinene, trans-Pinocarveol, and 1, 8-cineole. The antimicrobial investigation revealed that both essential oils displayed significant antibacterial efficacy against all tested organisms. J. procera demonstrated antibacterial activity ranging from 30.5 ± 0.70 to 24.0 ± 1.41 mm, surpassing that of C. camphora, with inhibition zones ranging from 19.5 ± 0.70 to 12.5 ± 0.70 mm. The essential oils were comparable to the reference antibiotic (chloramphenicol). Gram-positive bacteria exhibited higher susceptibility than Gram-negative bacteria. The insecticidal effects of essential oils on Aphis craccivora were studied, and results indicate increased death rates in a concentration-dependent manner when exposed to essential oils. The use of J. procera and C. camphora oils at concentrations below lethal levels was found to affect important biological factors in A. craccivora, including generation time, net reproductive rate, intrinsic rate of increase, and finite rate of growth. This suggests the potential of these oils to be effective agents for controlling pests. The multifaceted bioactivities of J. procera and C. camphora's essential oils highlight their promising potential as natural alternatives for antimicrobial and pest control applications.

The growing global demand for phytochemicals as bioactive sources is prompting scientists to develop methods that link their sensory properties to their mechanisms of action in cancer treatment. Recent techniques for tracking the actions of small plant metabolites (SPMs) from single-cell plant sources to their molecular anticancer biomarkers could provide valuable insights in this field. Among the critical methods discussed in this review are the real-time tracking of cell components through stable isotope probing (Sis) and microspectroscopy, which has attracted the attention of biotechnologists. Additionally, the precise pathways required for studying new insights into functional materials are discussed, based on high-resolution and accurate technologies, which could aid their functional categorization. Notably, the molecules under study have recently garnered attention for their anticancer applications due to advancements in effective evaluation techniques that surpass traditional methods. In December 2020, the Food and Drug Administration (FDA) authorized 89 SPMs as safe anticancer natural molecules. In conclusion, by combining spatiotemporal techniques and SPMs’ mechanisms, they could facilitate the development of more exceptional, bio-efficient materials.

Mentha piperita and Lavandula multifida are widely used in Moroccan traditional medicine for the treatment of diabetes and infectious diseases. The aims of this work were the determination of the chemical composition of Mentha piperita (MPEO) and Lavandula multifida (LMEO) essential oils and the evaluation of their antibacterial, antioxidant, and antidiabetic activities. The chemical composition was determined by GC-MS analysis. The antibacterial effects were evaluated against several bacterial strains using disc diffusion, MIC, and MBC methods. The antioxidant activity was evaluated in vitro using DPPH, H2O2, and xanthine oxidase, and the antidiabetic activity was estimated by the inhibitory effects of α-amylase, α-glucosidase, and lipase activities. GC-MS results showed that the main compounds of MPEO were menthone (29.24%), levomenthol (38.73%), and eucalyptol (6.75%). However, eucalyptol (28.11%), 2-bornanone (11.57%), endo-borneol (7.82%), and linalyl acetate (5.22%) are the major compounds of LMEO. The results exhibited important inhibitory effects against some bacterial strains with MIC = MBC = 0.39 mg/mL for MPEO against Staphylococcus aureus ATCC. However, LMEO exhibited remarkable antioxidant and antidiabetic activities compared to MPEO. Indeed, LMEO inhibited DPPH, H2O2, and xanthine oxidase with concentrations of 15.23, 21.52, and 8.89 µg/mL, respectively. Moreover, LMEO exhibited α-amylase and α-glucosidase at IC50 = 85.34 and IC50 = 59.36 µg/mL, respectively. The findings showed that both MPEO and LMEO exhibit promising biological properties. However, the application of these species or their main bioactive compounds requires further investigation.

Herbal plants represent a new source of hypoglycemic antidiabetic drugs; haematological and histopathological studies of methanol extract of Deverra tortuosa in streptozotocin-induced diabetic rats were investigated in vivo. A single intraperitoneal injection of 160 mg/kg bodyweights of streptozotocin was used to cause diabetes. Blood glucose levels were tested with an AccuCheck Advantage II glucometer and blood glucose test strips. After diabetes was confirmed, animals were orally treated with the extract, metformin, and insulin according to the experimental design. After extract therapy, histological alterations in the pancreas of diabetic rats were investigated. When compared to a control group, daily oral administration of D. tortuosa extract (300 mg/kg body weight) plus metformin (100 mg/kg) had a positive effect on blood glucose levels as well as showed an increased number of white blood cells (WBCs) and red blood cells (RBCs). The treatment with the extract for two weeks showed a positive impact on pancreatic histopathological changes in the groups with the diabetic rats. Phenolic fraction of the methanol extract was screened by the liquid chromatography-mass spectroscopy (LC-MS) method, which unveiled the existence of flavonoid compounds and phenolics as kaempferol, rutin, isorhamnetin-3-O-rutinoside, caffeic acid, and 4-hydroxybenzoic acid 4-O-glucoside. The results confirmed the use of the plant as an antidiabetic agent; the research recommended further studies on the plant to use the plant as an antidiabetic drug, where the plant extract also showed improvement in blood parameters.

Drought stress and drastic condition of northern region especial high temperature in summer season affect widely on the chemical component of plant and its biological actively plant grow in these condition adapt to this stress by accumulation different compound, thugia it is tree grow in garden and farms, when it grows in desert habitat show different content, Gc-Ms analysis of the terpenoid fraction of the plant where it is best fraction given high antimicrobial and Larvicidal activity was done and show the presence of many promising diterpene compounds, Ferruginol, Torulosol, dihydro, 18-Oxo-kauran-17-yl acetate and Andrographolide. The antibacterial testing showed that, the tested gram-positive bacteria exhibited good susceptibility to the terpenoid fraction of methanol extract, which was Staphylococcus epidermidis and Staphylococcus aureus, recorded 13.0±0.7 and 11.0±0.0 mm inhibition zones, respectively. Indicating promising antibacterial activity against the gram-positive pathogens. While all the tested gram-negative bacteria recorded weak or no susceptibility. The larvicidal activity of plant was studied on Spodoptera littoralis , The lethal concentration was 27.63 % of terpenoid fraction of Thuja orientalis. The plant can considered as a good herbal insecticide instead of synthetic insect side after further studies where the IC50: Botanical pesticides are highly effective, and ecologically acceptable.

Drought stress and drastic condition of northern region especial high temperature in summer season affect widely on the chemical component of plant and its biological actively plant grow in these condition adapt to this stress by accumulation different compound, thugia it is tree grow in garden and farms, when it grows in desert habitat show different content, Gc-Ms analysis of the terpenoid fraction of the plant where it is best fraction given high antimicrobial and Larvicidal activity was done and show the presence of many promising diterpene compounds, Ferruginol, Torulosol, dihydro, 18-Oxo-kauran-17-yl acetate and Andrographolide. The antibacterial testing showed that, the tested gram-positive bacteria exhibited good susceptibility to the terpenoid fraction of methanol extract, which was Staphylococcus epidermidis and Staphylococcus aureus, recorded 13.0±0.7 and 11.0±0.0 mm inhibition zones, respectively. Indicating promising antibacterial activity against the gram-positive pathogens. While all the tested gram-negative bacteria recorded weak or no susceptibility. The larvicidal activity of plant was studied on Spodoptera littoralis , The lethal concentration was 27.63 % of terpenoid fraction of Thuja orientalis. The plant can considered as a good herbal insecticide instead of synthetic insect side after further studies where the IC50: Botanical pesticides are highly effective, and ecologically acceptable.

Background We aimed to assess serum 25-hydroxyvitamin D3 (25(OH)D3) concentrations in extrapulmonary tuberculosis (EPTB) patients and to evaluate the effect of vitamin D3 supplementation on their treatment course. Methods Serum 25(OH)D3concentrations were measured in 47 newly diagnosed EPTB patients and 42 controls. Vitamin D-deficient EPTB patients were randomly assigned to receive 50,000 IU of vitamin D3 (cholecalciferol) orally once a week for 6 weeks (total 300,000 IU), followed by maintenance doses of 1000 IU a day besides anti-TB drugs or the first line anti-TB treatment only. Follow up serum 25(OH)D3 concentrations were measured after 3 months of starting vitamin D3 supplementation. Both groups were evaluated for clinical, laboratory, and radiological outcomes after treatment. Results Serum 25(OH)D3 concentrations were significantly lower among TB cases (17.1 ± 5.5 nmol/L) compared to healthy controls (51.8 ± 27.3 nmol/L), and vitamin D deficiency was observed in all EPTB patients ( n  = 47). Patients in VD3 supplementation group had significantly higher weight gain and serum albumin level at 2 months and end of treatment, higher hemoglobin concentration at the end of treatment, significantly lower CRP and ESR at 2 months and at the end of treatment. In cases with TB pleurisy, a significant higher rate of full resolution of pleural fluid after 6 months of anti-TB treatment and shorter treatment duration were noted compared to the other group. Conclusions Vitamin D deficiency is prevalent in EPTB patients, in whom, vitamin D supplementation is a useful adjunctive therapy to anti-TB drugs and improves treatment course.

Background: Alzheimer’s disease (AD) is largely linked with oxidative stress, the accumulation of amyloid-β plaques, and hyperphosphorylated τ-protein aggregation. Alterations in dopaminergic and serotonergic neurotransmission have also been implicated in various AD-related symptoms. Methods: To explore new therapeutic agents, a series of bicyclic and tricyclic thieno-oxazepine derivatives were synthesized as potential acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitors. The resultant compounds were purified via HPLC and characterized using spectral analysis techniques. Histopathological examinations, other antioxidants, and anti-inflammatory biomarkers were evaluated, and in silico ADMET calculations were performed for synthetic hybrids. Molecular docking was utilized to validate the new drugs’ binding mechanisms. Results: The most powerful AChE inhibitors were 14 and 16, with respective values of IC50 equal to 0.39 and 0.76 µM. Derivative 15 demonstrated remarkable BChE-inhibitory efficacy, on par with tacrine, with IC50 values of 0.70 µM. Hybrids 13 and 15 showed greater selectivity towards BChE, despite substantial inhibition of AChE. Compounds 13 and 15 reduced escape latency and raised residence time, with almost equal activity to donepezil. Conclusions: According to these findings, the designed hybrids constitute multipotent lead compounds that could be used in the creation of novel anti-AD medications.

Neuropathy is one of most common complications in diabetic patients. Diagnosis of diabetic neuropathy is essential for decreasing the rate of the disability and death. Neuron-specific enolase (NSE) is released from damaged neuronal cells and enters the blood circulation through an injured blood brain barrier. Therefore, serum NSE can reflect the damage of neurons and brain tissue. To evaluate peripheral polyneuropathy and cognitive function in Type 2 Diabetes Mellitus (T2DM) and correlate them with NSE level as a possible biomarker of diabetic neuropathy. Forty five T2DM patients with polyneuropathy were randomly recruited in this study compared to 45 healthy age and sex matched subjects as a control. Patients group were divided into two subgroups, 24 diabetic patients with painful peripheral neuropathy and 21 with painless peripheral neuropathy. All were subjected to clinical assessment by diabetic neuropathy symptom score, Dyck neuropathy grading, Mini-Mental State Examination (MMSE), assessment of HbA1c, NSE biomarker and neurophysiological assessment (nerve conduction study (NCS), event related potential (P300wave) and somatosensory evoked potential (SSEP) of the right median nerve). There were significant decrease in cognitive functions in diabetic patients compared to controls and a significant increase in NSE in diabetic patients. There were no significant difference between patients with painless and painful diabetic neuropathy as regard MMSE, HbA1c and NSE. There were significant correlation of P300 in diabetic patients with HbA1c and NSE. Neurophysiological assessment of diabetic patients by NCS, SSEP and P300 have well evaluation of cognitive functions, painless, and painful diabetic polyneuropathy. NSE is a beneficial biomarker in diabetic patients to pick up neurological complications.