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A novel strain Vibrio aphrogenes sp. nov. strain CA-1004T isolated from the surface of seaweed collected on the coast of Mie Prefecture in 1994 [1] was characterized using polyphasic taxonomy including multilocus sequence analysis (MLSA) and a genome based comparison. Both phylogenetic analyses on the basis of 16S rRNA gene sequences and MLSA based on eight protein-coding genes (gapA, gyrB, ftsZ, mreB, pyrH, recA, rpoA, and topA) showed the strain could be placed in the Rumoiensis clade in the genus Vibrio. Sequence similarities of the 16S rRNA gene and the multilocus genes against the Rumoiensis clade members, V. rumoiensis, V. algivorus, V. casei, and V. litoralis, were low enough to propose V. aphrogenes sp. nov. strain CA-1004T as a separate species. The experimental DNA-DNA hybridization data also revealed that the strain CA-1004T was separate from four known Rumoiensis clade species. The G+C content of the V. aphrogenes strain was determined as 42.1% based on the genome sequence. Major traits of the strain were non-motile, halophilic, fermentative, alginolytic, and gas production. A total of 27 traits (motility, growth temperature range, amylase, alginase and lipase productions, and assimilation of 19 carbon compounds) distinguished the strain from the other species in the Rumoiensis clade. The name V. aphrogenes sp. nov. is proposed for this species in the Rumoiensis clade, with CA-1004T as the type strain (JCM 31643T = DSM 103759T).

[This corrects the article DOI: 10.1371/journal.pone.0180053.].

Theranostics is a term coined by combining the words “therapeutics” and “diagnostics,” referring to single chemical entities developed to deliver therapy and diagnosis simultaneously. Neuroendocrine tumors are rare cancers that occur in various organs of the body, and they express neuroendocrine factors such as chromogranin A and somatostatin receptor. Somatostatin analogs bind to somatostatin receptor, and when combined with diagnostic radionuclides, such as gamma‐emitters, are utilized for diagnosis of neuroendocrine tumor. Somatostatin receptor scintigraphy when combined with therapeutic radionuclides, such as beta‐emitters, are effective in treating neuroendocrine tumor as peptide receptor radionuclide therapy. Somatostatin receptor scintigraphy and peptide receptor radionuclide therapy are some of the most frequently used and successful theranostics for neuroendocrine tumor. In Japan, radiopharmaceuticals are regulated under a complex law system, creating a significant drug lag, which is a major public concern. It took nearly 10 years to obtain the approval for somatostatin receptor scintigraphy and peptide receptor radionuclide therapy use by the Japanese government. In 2021, 111Lu‐DOTATATE (Lutathera), a drug for peptide receptor radionuclide therapy, was covered by insurance in Japan. In this review, we summarize the history of the development of neuroendocrine tumor theranostics and theranostics in general, as therapeutic treatment for cancer in the future. Furthermore, we briefly address the Japanese point of view regarding the development of new radiopharmaceuticals. Theranostics for neuroendocrine tumor.

Plastoquinone plays a crucial role in the photosynthetic electron transport system as an electron carrier, transferring electrons from photosystem II to cytochrome b 6 f complexes. Certain cyanobacteria acylate plastoquinone derivatives, plastoquinol, the reduced form of plastoquinone, and/or plastoquinone-C, the hydroxylated form of plastoquinone to synthesize newly found cyanobacterial lipids, acylplastoquinol and acylplastoquinone-C, the latter of which is known as plastoquinone-B in seed plants. The cyanobacterial genes, slr2103 in Synechocystis sp. PCC 6803 and its ortholog in Synechococcus sp. PCC 7002, encode a bifunctional acyltransferase for the synthesis of both acylplastoquinol and plastoquinone-B. Despite conservation of slr2103 orthologs across a wide range of cyanobacteria, only four cyanobacterial strains, including the two mentioned above, have been identified as producing acylplastoquinol and/or plastoquinone-B. Moreover, the extent to which acylplastoquinone species are distributed in eukaryotic photosynthetic organisms that lack slr2103 orthologs remains largely unknown. Using LC-MS/MS 2 analysis of total cellular lipids, this study demonstrates that acylplastoquinol and plastoquinone-B are conserved not only in cyanobacteria with slr2103 orthologs but also in eukaryotic photosynthetic organisms lacking these orthologs, including primary and secondary endosymbiotic algae, and a seed plant. Notably, in eukaryotic photosynthetic organisms as well as in cyanobacteria, these acylplastoquinone species are predominantly esterified with saturated fatty acids. The evolutionary conservation of these acylplastoquinone species suggests replacement of slr2103 orthologs by alternative gene(s) responsible for their synthesis at least once after the primary endosymbiotic event in the evolution of photosynthetic organisms. The persistent conservation of acylplastoquinone species throughout the evolution likely reflects their critical physiological roles.

Mammalian artificial chromosomes derived from native chromosomes have been applied to biomedical research and development by generating cell sources and transchromosomic (Tc) animals. Human artificial chromosome (HAC) is a precedent chromosomal vector which achieved generation of valuable humanized animal models for fully human antibody production and human pharmacokinetics. While humanized Tc animals created by HAC vector have attained significant contributions, there was a potential issue to be addressed regarding stability in mouse tissues, especially highly proliferating hematopoietic cells. Mouse artificial chromosome (MAC) vectors derived from native mouse chromosome 11 demonstrated improved stability, and they were utilized for humanized Tc mouse production as a standard vector. In mouse, however, stability of MAC vector derived from native mouse chromosome other than mouse chromosome 11 remains to be evaluated. To clarify the potential of mouse centromeres in the additional chromosomes, we constructed a new MAC vector from native mouse chromosome 10 to evaluate the stability in Tc mice. The new MAC vector was transmitted through germline and stably maintained in the mouse tissues without any apparent abnormalities. Through this study, the potential of additional mouse centromere was demonstrated for Tc mouse production, and new MAC is expected to be used for various applications.

Polyphosphate is prevalent in living organisms. To obtain insights into polyphosphate synthesis and its physiological significance in cyanobacteria, we characterize sll0290 , a homolog of the polyphosphate-kinase-1 gene, in the freshwater cyanobacterium Synechocystis sp. PCC 6803. The Sll0290 protein structure reveals characteristics of Ppk1. A Synechocystis sll0290 disruptant and sll0290 -overexpressing Escherichia coli transformant demonstrated loss and gain of polyphosphate synthesis ability, respectively. Accordingly, sll0290 is identified as ppk1 . The disruptant (Δ ppk1 ) grows normally with aeration of ordinary air (0.04% CO 2 ), consistent with its photosynthesis comparable to the wild type level, which contrasts with a previously reported high-CO 2 (5%) requirement for Δ ppk1 in an alkaline hot spring cyanobacterium, Synechococcus OS-B’. Synechocystis Δ ppk1 is defective in polyphosphate hyperaccumulation and survival competence at the stationary phase, and also under sulfur-starvation conditions, implying that sulfur limitation is one of the triggers to induce polyphosphate hyperaccumulation in stationary cells. Furthermore, Δ ppk1 is defective in the enhancement of total phosphorus contents under sulfur-starvation conditions, a phenomenon that is only partially explained by polyphosphate hyperaccumulation. This study therefore demonstrates that in Synechocystis , ppk1 is not essential for low-CO 2 acclimation but plays a crucial role in dynamic P-metabolic regulation, including polyP hyperaccumulation, to maintain physiological fitness under sulfur-starvation conditions.

Background The white matter damage inducing the reemergence of grasp reflexes and their potential lateralization remains unstudied. Idiopathic normal pressure hydrocephalus (iNPH), a subcortical dementia, is an ideal model for these investigations. We aim to understand the contributions of white matter to the inhibition of grasp reflexes in patients with iNPH. Methods A total of 48 patients (mean age at admission: 77.8 ± 5.2 years; 56% male) with probable iNPH were retrospectively enrolled in this study. The intensity of grasp reflexes was assessed using a four-category classification. A voxel-based morphometric analysis of the fractional anisotropy (FA) maps was conducted to identify responsible regions related to the grasp reflex. The white matter fibers passing through these regions were tracked using fiber-tracking data from fifty age-/sex-matched healthy subjects from the Lifespan Human Connectome Project Aging Study. Fibers with an inter-subject overlap rate > 50% were defined as reliable tracts for further discussion. Results Positive grasp reflex was identified in 60% (29/48) of probable iNPH cases. The voxel-based multiple regression analysis revealed that the reflex intensity scores were negatively correlated with FA values in the right frontal subcortical white matter near the anterior horn of the right lateral ventricle. The white matter fibers project through this structure mainly to the posterior parts of the right superior, middle, and inferior frontal gyri; the bilateral presupplementary motor areas; the right dorsal anterior cingulate cortex; the ventral lateral nuclei of the bilateral thalami; the pulvinar inferior nucleus of the right thalamus; the lateral part of the right putamen; and the right subthalamic nucleus. Conclusion The inhibition of grasp reflexes is achieved via a right-lateralized prefrontal cortex–basal ganglia–thalamocortical ‘stopping’ network.

•ECoG high‐gamma analysis reveals distinct spatiotemporal reading dynamics.•Kanji reading activates bilateral ventral occipitotemporal cortex early.•Kana reading shows prolonged activation in the left dorsal pathway.•Distinct neural circuits mediate Kanji versus Kana processing. Reading engages complex neural networks integrating visual, phonological, and semantic information. The dual-stream model posits ventral and dorsal pathways for lexical and sublexical processing in the left hemisphere and is well-supported in alphabetic languages. However, its applicability to non-alphabetic scripts remains unclear. The Japanese writing system, comprising Kana (syllabograms) and Kanji (morphograms) with distinct orthographic, phonological, and semantic properties, provides a unique framework to investigate neural dissociation between phonological and orthographic-semantic processing. Previous studies suggest that Kanji relies on the ventral route for whole-word recognition and semantic processing, whereas Kana depends mainly on the dorsal route for phonological decoding via grapheme-to-phoneme conversion; however, their spatiotemporal dynamics remain unknown. Using high-gamma power analysis from electrocorticography recordings in 14 patients with epilepsy and subdural implants, we examined the spatiotemporal neural dynamics of Kana and Kanji reading. Participants completed a visual lexical decision task with Kana and Kanji words and pseudowords. Across 912 electrodes, differential high-gamma power analysis showed that Kanji activated bilateral occipitotemporal fusiform regions early (120–550 ms) and the left inferior temporal gyrus (150–240 ms). Conversely, Kana showed prolonged late activation (270–750 ms) in the left-lateralised superior temporal, supramarginal, and inferior frontal gyri, especially during pseudoword processing. These findings indicate that Kanji relies on bilateral ventral stream earlier, while Kana depends on the left dorsal stream, with slower processing reflecting the extra grapheme-to-phoneme conversion. This underscores the value of non-alphabetic languages in elucidating both universal and script-specific neural mechanisms, advancing a cross-linguistic understanding of the reading network. [Display omitted]

Background There has been insufficient evidence regarding a treatment strategy for patients with non-occlusive mesenteric ischemia (NOMI) due to the lack of large-scale studies. We aimed to evaluate the clinical benefit of strategic planned relaparotomy in patients with NOMI using detailed perioperative information. Methods We conducted a multicenter retrospective cohort study that included NOMI patients who underwent laparotomy. In-hospital mortality, 28-day mortality, incidence of total adverse events, ventilator-free days, and intensive care unit (ICU)–free days were compared between groups experiencing the planned and on-demand relaparotomy strategies. Analyses were performed using a multivariate mixed effects model and a propensity score matching model after adjusting for pre-operative, intra-operative, and hospital-related confounders. Results A total of 181 patients from 17 hospitals were included, of whom 107 (59.1%) were treated using the planned relaparotomy strategy. The multivariate mixed effects regression model indicated no significant differences for in-hospital mortality (61 patients [57.0%] in the planned relaparotomy group vs. 28 patients [37.8%] in the on-demand relaparotomy group; adjusted odds ratio [95% confidence interval] = 1.94 [0.78–4.80]), as well as in 28-day mortality, adverse events, and ICU-free days. Significant reduction in ventilator-free days was observed in the planned relaparotomy group. Propensity score matching analysis of 61 matched pairs with comparable patient severity did not show superiority of the planned relaparotomy strategy. Conclusions The planned relaparotomy strategy, compared with on-demand relaparotomy strategy, did not show clinical benefits after the initial surgery of patients with NOMI. Further studies estimating potential subpopulations who may benefit from this strategy are required.