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To determine that 1) an age-dependent loss of inducible autophagy underlies the failure to recover from AKI in older, adult animals during endotoxemia, and 2) pharmacologic induction of autophagy, even after established endotoxemia, is of therapeutic utility in facilitating renal recovery in aged mice. Murine model of endotoxemia and cecal ligation and puncture (CLP) induced acute kidney injury (AKI). Academic research laboratory. C57Bl/6 mice of 8 (young) and 45 (adult) weeks of age. Lipopolysaccharide (1.5 mg/kg), Temsirolimus (5 mg/kg), AICAR (100 mg/kg). Herein we report that diminished autophagy underlies the failure to recover renal function in older adult mice utilizing a murine model of LPS-induced AKI. The administration of the mTOR inhibitor temsirolimus, even after established endotoxemia, induced autophagy and protected against the development of AKI. These novel results demonstrate a role for autophagy in the context of LPS-induced AKI and support further investigation into like interventions that have potential to alter the natural history of disease.

Denervation of skeletal muscles induces severe muscle atrophy, which is preceded by cellular alterations such as increased plasma membrane permeability, reduced resting membrane potential and accelerated protein catabolism. The factors that induce these changes remain unknown. Conversely, functional recovery following denervation depends on successful reinnervation. Here, we show that activation of nicotinic acetylcholine receptors (nAChRs) by quantal release of acetylcholine (ACh) from motoneurons is sufficient to prevent changes induced by denervation. Using in vitro assays, ACh and non-hydrolysable ACh analogs repressed the expression of connexin43 and connexin45 hemichannels, which promote muscle atrophy. In co-culture studies, connexin43/45 hemichannel knockout or knockdown increased innervation of muscle fibers by dorsal root ganglion neurons. Our results show that ACh released by motoneurons exerts a hitherto unknown function independent of myofiber contraction. nAChRs and connexin hemichannels are potential molecular targets for therapeutic intervention in a variety of pathological conditions with reduced synaptic neuromuscular transmission. Denervation of muscle fibres induces muscle atrophy, via mechanisms that remain unclear. Here, the authors show that binding of acetylcoline to its receptor at the neuromuscular junction represses the expression of connexins 43 and 45, which promote atrophy, and is sufficient to prevent denervation-induced loss of myofibre mass.

Panoramic dental radiography is one of the most used images of the different dental specialties. This radiography provides information about the anatomical structures of the teeth. The correct evaluation of these radiographs is associated with a good quality of the image obtained. In this study, 598 patients were consecutively selected to undergo dental panoramic radiography at the Department of Radiology of the Faculty of Dentistry, Universidad Nacional de Asunción. Contrast enhancement techniques are used to enhance the visual quality of panoramic dental radiographs. Specifically, this article presents a new algorithm for contrast, detail and edge enhancement of panoramic dental radiographs. The proposed algorithm is called Multi-Scale Top-Hat transform powered by Geodesic Reconstruction for panoramic dental radiography enhancement (MSTHGR). This algorithm is based on multi-scale mathematical morphology techniques. The proposal extracts multiple features of brightness and darkness, through the reconstruction of the marker (obtained by the Top-Hat transformation by reconstruction) starting from the mask (obtained by the classic Top-Hat transformation). The maximum characteristics of brightness and darkness are added to the dental panoramic radiography. In this way, the contrast, details and edges of the panoramic radiographs of teeth are improved. For the tests, MSTHGR was compared with the following algorithms: Geodesic Reconstruction Multiscale Morphology Contrast Enhancement (GRMMCE), Histogram Equalization (HE), Brightness Preserving Bi-Histogram Equalization (BBHE), Dual Sub-Image Histogram Equalization (DSIHE), Minimum Mean Brightness Error Bi-Histogram Equalization (MMBEBHE), Quadri-Histogram Equalization with Limited Contrast (QHELC), Contrast-Limited Adaptive Histogram Equalization (CLAHE) and Gamma Correction (GC). Experimentally, the numerical results show that the MSTHGR obtained the best results with respect to the Contrast Improvement Ratio (CIR), Entropy (E) and Spatial Frequency (SF) metrics. This indicates that the algorithm performs better local enhancements on panoramic radiographs, improving their details and edges.

Plant-based diets are increasingly popular in contemporary society. While they are suitable for all life stages, there is a potential risk of nutrient deficiencies, particularly in omega-3 fatty acids, vitamins A, D, and B12, zinc, iodine, selenium, choline, and creatine. During pregnancy and lactation, the nutritional demands increase significantly, making proper supplementation essential. Breastfeeding remains the optimal feeding method for infants, provided key nutrient needs are met through supplementation. A bibliographic search in Scopus, Web of Science, and PubMed focused on plant-based diets, supplementation, and key nutrients over the past 10 years. Studies involving diseases or duplicates were excluded, and data were analyzed from European and U.S. sources to assess nutrient supplementation trends. Micronutrient deficiencies during pregnancy and lactation can negatively impact infant neurological development. Vitamin A supports vision and immunity, while vitamin D aids fetal bone mineralization. Deficiency in vitamin B12 can lead to anemia and neurological issues. Zinc, iodine, selenium, omega-3 fatty acids (EPA and DHA), choline, and creatine also play critical roles in development and may require supplementation in plant-based diets. With careful planning, plant-based diets can meet nutritional needs during pregnancy, lactation, and childhood. Supplementation with key micronutrients, including choline and creatine, is essential for neurodevelopment and energy metabolism. Dietitians play a vital role in guiding individualized dietary plans, and further research is needed on optimal supplement dosages and long-term health effects. [Display omitted] •Plant-based diets and pregnancy▪▪—Popular but require careful planning to prevent nutrient deficiencies.•Key nutrients at risk▪—ω3, vitamins A, D, B12, zinc, iodine, selenium, choline and creatine.•Impact on infants▪▪—Deficiencies can affect neurological development, immunity and bone health.•Supplementation is essential▪—Proper intake ensures optimal fetal and infant health.

DNA barcoding, based on mitochondrial markers, is widely applied in species identification and biodiversity studies. The aim of this study was to establish a barcoding reference database of fishes inhabiting the Cube River from Western Ecuador in the Chocó-Darien Global Ecoregion (CGE), a threatened ecoregion with high diversity and endemism, and evaluate the applicability of using barcoding for the identification of fish species. Barcode sequences were obtained from seven orders, 17 families, 23 genera and 26 species, which were validated through phylogenetic analysis, morphological measurements, and literature review. Our results showed that 43% of fish species in this region are endemic, confirmed the presence of known species in the area, and included the addition of three new records of native ( Hoplias microlepis , Rhamdia guatemalensis and Sicydium salvini ) and an introduced species ( Xiphophorus maculatus ) to Ecuador. In addition, eight species were barcoded for the first time. Species identification based on barcoding and morphology showed discrepancy with species lists from previous studies in the CGE, suggesting that the current baseline of western fishes of Ecuador is still incomplete. Because this study analyzed fishes from a relatively small basin (165 km 2 ), more molecular-based studies focusing on fish are needed to achieve a robust sequence reference library of species inhabiting Western Ecuador. The new sequences of this study will be useful for future comparisons and biodiversity monitoring, supporting the application of barcoding tools for studying fish diversity in genetically unexplored regions and to develop well-informed conservation programs.

Draining peatlands for forestry in the northern hemisphere turns their soils from carbon sinks to substantial sources of greenhouse gases (GHGs). To reverse this trend, rewetting has been proposed as a climate change mitigation strategy. We performed a literature review to assess the empirical evidence supporting the hypothesis that rewetting drained forested peatlands can turn them back into carbon sinks. We also used causal loop diagrams (CLDs) to synthesize the current knowledge of how water table management affects GHG emissions in organic soils. We found an increasing number of studies from the last decade comparing GHG emissions from rewetted, previously forested peatlands, with forested or pristine peatlands. However, comparative field studies usually report relatively short time series following rewetting experiments (e.g., 3 years of measurements and around 10 years after rewetting). Empirical evidence shows that rewetting leads to lower GHG emissions from soils. However, reports of carbon sinks in rewetted systems are scarce in the reviewed literature. Moreover, CH 4 emissions in rewetted peatlands are commonly reported to be higher than in pristine peatlands. Long-term water table changes associated with rewetting lead to a cascade of effects in different processes regulating GHG emissions. The water table level affects litterfall quantity and quality by altering the plant community; it also affects organic matter breakdown rates, carbon and nitrogen mineralization pathways and rates, as well as gas transport mechanisms. Finally, we conceptualized three phases of restoration following the rewetting of previously drained and forested peatlands, we described the time dependent responses of soil, vegetation and GHG emissions to rewetting, concluding that while short-term gains in the GHG balance can be minimal, the long-term potential of restoring drained peatlands through rewetting remains promising.

Reactive oxygen species (ROS) are intermediates in oxidation–reduction reactions with the capacity to modify biomolecules and temporarily or permanently alter cell behaviour through signalling pathways under physiological and pathophysiological conditions where there is an imbalance between oxidative factors and the antioxidant response of the organism, a phenomenon known as oxidative stress. Evidence suggests that the differential modulation of ROS-mediated oxidative stress occurs in the pathogenesis and progression of melanoma, and that this imbalance in redox homeostasis appears to be functionally linked to microRNA (miRNA o miRs)-mediated non-mutational epigenetic reprogramming involving genes and transcription factors. The relationship between ROS-mediated stress control, tumour microenvironment, and miRNA expression in melanoma is not fully understood. The aim of this review is to analyse the involvement of miRNAs in the modulation of the signalling pathways involved in ROS-mediated oxidative stress in melanoma. It is hoped that these considerations will contribute to the understanding of the mechanisms associated with a potential epigenetic network regulation, where the modulation of oxidative stress is consolidated as a common factor in melanoma, and therefore, a potential footprint poorly documented.

New asphalt mixtures have been improved by using fibers (polypropylene, polyester, asbestos, carbon, glass, nylon, lignin, coconut, sisal, recycled rubber, PET, wood, bamboo, and cellulose), reducing the temperature and compaction energy for their collocation, minimizing the impact on the environment, increasing the tenacity and resistance to cracking of hot mix asphalt (HMA), preventing asphalt drainage in a Stone Mastic Asphalt (SMA). Hence, this paper aims to evaluate the influence of the chemical (lignin content, ash, viscosity, degree of polymerization, and elemental analysis), morphological (SEM), spectroscopic (FTIR-ATR and XRD), and calorimetric (ATG and DSC) properties of celluloses from bagasse Agave tequilana Weber var. Azul (ABP), corrugated paperboard (CPB) and commercial cellulose fiber (CC) as Schellenberg drainage (D) inhibitors of the SMA. The ABP was obtained through a chemical process by alkaline cooking, while CPB by a mechanical refining process. The chemical, morphological, spectroscopic, and calorimetric properties were similar among the analyzed celluloses, but CPB and ABP cellulose are excellent alternatives to CC cellulose for inhibiting drainage. However, CPB is the most effective at low concentrations. This is attributed to its morphology, which includes roughness, waviness, filament length, orientation, and diameter, as well as its lignin content and crystallinity.

Electric vehicles, particularly those in mass transit systems, make use of accurate power estimations for different routes to calculate powertrain and battery requirements and plan the location and times of charging stations. Hence, chassis dynamometers are a common tool for vehicle designers as they allow for the emulation of vehicle performance and energy consumption by simulating realistic road conditions. In this paper, a method is presented where inertia events and negative slopes can be represented in the dynamometer through a single motor; allowing researchers to perform fast and cheap tests, while also considering the effect of these variables. A dynamic simulation is used to distribute the energy used in three ways: first, accelerating the vehicle by overcoming the forces opposing motion; second, emulating the kinetic energy delivered by the vehicle mass when decelerating; and third, emulating the energy delivered to the vehicle by negative slopes. Tests were carried out on a dynamometer validating the method through an example route, estimating energy consumption and regeneration; this method reduces the error in energy consumption by inertial effects and negative slopes, otherwise not considered in one motor dynamometers, showing a 9.11% difference between total test energy and real bus energy for this route.

The development of vaccine adjuvants is of interest for the management of chronic diseases, cancer, and future pandemics. Therefore, the role of Toll-like receptors (TLRs) in the effects of vaccine adjuvants has been investigated. TLR4 ligand-based adjuvants are the most frequently used adjuvants for human vaccines. Among TLR family members, TLR4 has unique dual signaling capabilities due to the recruitment of two adapter proteins, myeloid differentiation marker 88 (MyD88) and interferon-β adapter inducer containing the toll-interleukin-1 receptor (TIR) domain (TRIF). MyD88-mediated signaling triggers a proinflammatory innate immune response, while TRIF-mediated signaling leads to an adaptive immune response. Most studies have used lipopolysaccharide-based ligands as TLR4 ligand-based adjuvants; however, although protein-based ligands have been proven advantageous as adjuvants, their mechanisms of action, including their ability to undergo structural modifications to achieve optimal immunogenicity, have been explored less thoroughly. In this work, we characterized the effects of two protein-based adjuvants (PBAs) on TLR4 signaling via the recruitment of MyD88 and TRIF. As models of TLR4-PBAs, we used hemocyanin from Fissurella latimarginata (FLH) and a recombinant surface immunogenic protein (rSIP) from Streptococcus agalactiae . We determined that rSIP and FLH are partial TLR4 agonists, and depending on the protein agonist used, TLR4 has a unique bias toward the TRIF or MyD88 pathway. Furthermore, when characterizing gene products with MyD88 and TRIF pathway-dependent expression, differences in TLR4-associated signaling were observed. rSIP and FLH require MyD88 and TRIF to activate nuclear factor kappa beta (NF-κB) and interferon regulatory factor (IRF). However, rSIP and FLH have a specific pattern of interleukin 6 (IL-6) and interferon gamma-induced protein 10 (IP-10) secretion associated with MyD88 and TRIF recruitment. Functionally, rSIP and FLH promote antigen cross-presentation in a manner dependent on TLR4, MyD88 and TRIF signaling. However, FLH activates a specific TRIF-dependent signaling pathway associated with cytokine expression and a pathway dependent on MyD88 and TRIF recruitment for antigen cross-presentation. Finally, this work supports the use of these TLR4-PBAs as clinically useful vaccine adjuvants that selectively activate TRIF- and MyD88-dependent signaling to drive safe innate immune responses and vigorous Th1 adaptive immune responses.