Explore the vast range of titles available.

Chromophobe renal cell carcinoma (chRCC) is a rare subtype of renal cell carcinoma (RCC) and is the most common form of non-clear cell renal cell carcinoma in young women. Compared to clear cell renal cell carcinoma (ccRCC), chRCC usually has an excellent prognosis, indicating the need for a reliable differential diagnosis, especially to distinguish it from eosinophilic variants of ccRCC. Another important differential diagnosis is renal oncocytoma (RO), which remains a major challenge even for experienced pathologists. The treatment of RO typically involves active surveillance, with surgical resection indicated if there is significant tumor growth. In contrast, for chRCC, the approach depends on tumor size, with either partial or radical nephrectomy being required. This review therefore summarizes key unique features and recent findings on this tumor, aiming to ensure a reliable differential diagnosis, thereby facilitating appropriate treatment selection and prognosis assessment. The histology of chRCC, including both for the classic and the eosinophilic subtype, is characterized by the appearance of raisinoid cell nuclei with perinuclear halos on microscopic imaging. In rare cases, signs of sarcomatoid, glandular and/or anaplastic dedifferentiation can also be observed, which significantly worsens the prognosis. The immunohistochemical marker phospho-S6 can be used to detect these changes. In addition to other routinely used markers such as C-Kit, CK7, EpCAM, CAIX and Claudin 7, we recommend the use of progesterone receptors as markers, as many chRCC express them and are thus progesterone-sensitive. This progesterone sensitivity could indicate that chRCC, similar to breast cancer, may represent a contraindication for the use of hormonal contraceptives. In addition to immunohistochemistry, molecular features of chRCC such as genetic, epigenetic, transcriptomic and proteomic alterations can be considered in the differential diagnosis. In this review, we therefore outline the most important established alterations in this context. In the treatment of metastatic chRCC, checkpoint inhibitors and tyrosine kinase inhibitors have demonstrated efficacy and may represent a promising new approach for managing dedifferentiated, aggressive or metastatic chRCC. This review aims to present recent therapeutic advances and provide innovative approaches for future clinical treatment decisions.

Due to its dire impacts on marine life, public health, and socio-economic services, oil spills require an immediate response. Effective action starts with good knowledge of the ocean dynamics and circulation, from which Lagrangian methods derive key information on the dispersal pathways present in the contaminated region. However, precise assessments of the capacity of Lagrangian methods in real contamination cases remain rare and limited to large slicks spanning several hundreds of km. Here we address this knowledge gap and consider two medium-scale (tens of km wide) events of oil in contrasting conditions: an offshore case (East China Sea, 2018) and a recent near-coastal one (East Mediterranean, 2021). Our comparison between oil slicks and Lagrangian diagnostics derived from near-real-time velocity fields shows that the calculation of Lagrangian fronts is, in general, more robust to errors in the velocity fields and more informative on the dispersion pathways than the direct advection of a numerical tracer. The inclusion of the effect of wind is also found to be essential, being capable of suddenly breaking Lagrangian transport barriers. Finally, we show that a usually neglected Lagrangian quantity, the Lyapunov vector, can be exploited to predict the front drifting speed, and in turn, its future location over a few days, on the basis of near-real-time information alone. These results may be of special relevance in the context of next-generation altimetry missions that are expected to provide highly resolved and precise near-real-time velocity fields for both open ocean and coastal regions.

Pufferfishes are among the best-known marine organisms that accumulate marine biotoxins such as Tetrodotoxin (TTX). In the Mediterranean Sea, the silver-cheeked toadfish Lagocephalus sceleratus is the most reported TTX-bearer, causing many fatal and non-fatal cases. In Lebanon, no previous studies have measured TTX levels although the possibility of TTX-poisoning is high since L. sceleratus is caught in different sizes and can be mistaken with other small fishes. Hence, this study reports TTX and its analogue 4,9-anhydro TTX in L. sceleratus collected from Lebanese waters in the Eastern Mediterranean Sea. The results show that TTX concentrations in fish tissues varied between 0.10 and 252.97 µg/g, while those of 4,9-anhydro TTX oscillated between 0.01 and 43.01 µg/g. Internal organs of L. sceleratus were the most toxic parts of its body, with the highest TTX levels found in gonads (mainly ovaries) and liver, followed by the muscles and skin with concentrations always exceeding the safety level. Toxicity fluctuations of L. sceleratus, its expansion, ecological and economic effects were also elucidated. Based on the present findings, it has been confirmed that L. sceleratus constitutes a health, ecological and economic risks, and therefore its trade in seafood markets should be banned to avoid any potential intoxication.

Coastal water quality preservation is very important for the well-being of both the local community and the marine environment. Fecal Bacteria (FC) contamination of coastal water is a serious environmental threat. Therefore, the analysis of FC is used to assess coastal waters for potential contamination of biota (e.g., shellfish) and threats to public health and is considered a very relevant pollution-indicating method for coastal waters. For that reason, a data-driven model was created based on several water quality parameters monitored throughout the Lebanese coastline. The created Artificial Neural Network (ANN) managed to capture well the tendency of the FC dynamics ( R =0.86 between the real and predicted data), while the sensitivity analysis revealed that the salinity is the most influential parameter on the FC parameter. Additionally, the water samples were categorized into safe and non-safe, and the ANN managed to classify the water quality data samples with high accuracy into these two categories (F1 score=94.24%). Therefore, the created ANN can act as a complementary management tool regarding coastal/bathing water safety indication.

Despite emerging and increasing concerns related to marine micro and macroplastics, no systematic surveys have been undertaken yet in the Lebanese marine area. To understand the spatio-temporal variation of plastic litter (macro and microplastics) in the Lebanese marine environment and to determine the sources of pollution, this study investigated the characteristics of plastic pollution in sea surface waters during wet and dry seasons in 22 sites of Beirut and Tyre regions. A total of 23,023 items were identified and assessed according to the shape, color, and concentration; moreover, the risk of microplastics (MPs) contamination was explored based on a risk assessment model. The obtained results demonstrated that the average macroplastics concentration was 0.45 ± 0.6 items/m 3 . The average microplastics concentration was found to be 20.1 ± 21.8 and 3.78 ± 5.2 items/m 3 in spring and fall respectively. During fall, MPs fragments were dominant in Beirut (97%) and Tyre (91%), and no pellets were observed. During spring, filaments were most encountered in Beirut (76.5%). The most dominant marine litter color was blue followed by black and white. The Pollution Load Index (PLI) values showed a moderate contamination of the Lebanese coast with MPs (PLI: 5.79 ± 3.93) except for several sites in Beirut that showed high values of PLI, highlighting the local influence of cities and rivers on MPs concentration. This study serves as an important baseline for understanding the characteristics of the seasonal variation of MPs along the Lebanese marine environment; it will help stakeholders and countries to take proactive and reactive actions to face plastic litter pollution in the Lebanese coastal area.

The Rhodes Gyre is a cyclonic persistent feature of the general circulation of the Levantine Basin in the eastern Mediterranean Sea. Although it is located in the most oligotrophic basin of the Mediterranean Sea, it is a relatively high primary production area due to strong winter nutrient supply associated with the formation of Levantine Intermediate Water. In this study, a 3D coupled hydrodynamic–biogeochemical model (SYMPHONIE/Eco3M-S) was used to characterize the seasonal and interannual variability of the Rhodes Gyre's ecosystem and to estimate an annual organic carbon budget over the 2013–2020 period. Comparisons of model outputs with satellite data and compiled in situ data from cruises and Biogeochemical-Argo floats revealed the ability of the model to reconstruct the main seasonal and spatial biogeochemical dynamics of the Levantine Basin. The model results indicated that during the winter mixing period, phytoplankton first progressively grow sustained by nutrient supply. Then, short episodes of convection driven by heat loss and wind events, favoring nutrient injections, organic carbon export, and inducing light limitation on primary production, alternate with short episodes of phytoplankton growth. The estimate of the annual organic carbon budget indicated that the Rhodes Gyre is an autotrophic area, with a positive net community production in the upper layer (0–150 m) amounting to 31.2 ± 6.9 gCm-2yr-1. Net community production in the upper layer is almost balanced over the 7-year period by physical transfers, (1) via downward export (16.8 ± 6.2 gCm-2yr-1) and (2) through lateral transport towards the surrounding regions (14.1 ± 2.1 gCm-2yr-1). The intermediate layer (150–400 m) also appears to be a source of organic carbon for the surrounding Levantine Sea (7.5 ± 2.8 gCm-2yr-1) mostly through the subduction of Levantine Intermediate Water following winter mixing. The Rhodes Gyre shows high interannual variability with enhanced primary production, net community production, and exports during years marked by intense heat losses and deep mixed layers. However, annual primary production appears to be only partially driven by winter vertical mixing. Based on our results, we can speculate that future increase of temperature and stratification could strongly impact the carbon fluxes in this region.

The eastern Mediterranean surface circulation is highly energetic and composed of structures interacting stochastically. However, some main features are still debated, and the behavior of some fine-scale dynamics and their role in shaping the general circulation is yet unknown. In the following paper, we use an unsupervised neural network clustering method to analyze the long-term variability of the different mesoscale structures. We decompose 26 years of altimetric data into clusters reflecting different circulation patterns of weak and strong flows with either strain or vortex-dominated velocities. The vortex-dominated cluster is more persistent in the western part of the basin, which is more active than the eastern part due to the strong flow along the coast, interacting with the extended bathymetry and engendering continuous instabilities. The cluster that reflects a weak flow dominated the middle of the basin, including the Mid-Mediterranean Jet (MMJ) pathway. However, the temporal analysis shows a frequent and intermittent occurrence of a strong flow in the middle of the basin, which could explain the previous contradictory assessment of MMJ existence using in-situ observations. Moreover, we prove that the Levantine Sea is becoming more and more energetic as the activity of the main mesoscale features is showing a positive trend.

Fil: Paparazzo, Flavio Emiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Centro Nacional Patagónico. Centro para el Estudio de Sistemas Marinos; Argentina

Solid lipid nanoparticles and nanostructure lipid carriers were used to entrap hesperetin and broaden confined knowledge of application of nanocarriers as the functional ingredients in food sectors. The produced nanocarriers using a high mechanical shear method were subjected to size and zeta potential analysis. The developed nanosize carriers had the encapsulation efficiency ranging from 39.90 to 63.08 %. Differential scanning calorimetry, X-ray diffraction, and Fourier transform infrared spectroscopy were also employed to study thermal behavior, crystalline state, and chemical structure. The release behavior of hesperetin in simulated gastrointestinal conditions was investigated and kinetically modeled. The modeling results indicated that the release phenomenon is mostly governed by combination of Fickian and dissolution mechanisms. Stability of the nanocarriers, as analyzed for up to 30 days, at 6 and 25 °C in aqueous suspension, showed no detectable hesperetin leakage. Cryoprotectant effect of different compounds (i.e., glucose, sorbitol, glycerin, lactose, and sucrose) was also examined. Finally, the potential capability of nanocarriers for food fortification was studied using milk as a model food. The fortified milk samples were subjected to sensory analysis and results betokened that the developed nanocarriers did not show any significant difference with blank milk sample and could well mask the bitter taste, after taste, and obviate poor solubility of hesperetin.