Explore the vast range of titles available.

Ovarian cancer is a highly aggressive malignancy with limited therapeutic options and a poor prognosis. Deubiquitinating enzymes (DUBs) have emerged as critical regulators of protein ubiquitination and proteasomal degradation, influencing various cellular processes relevant to cancer pathogenesis. In this study, the research progress between ovarian cancer and DUBs was mapped and visualized using bibliometrics, and the expression patterns and biological roles of DUBs in ovarian cancer were summarized. Studies related to DUBs in ovarian cancer were extracted from the Web of Science Core Collection (WoSCC) database. VOSviewer 1.6.20, CiteSpace 6.3.R1, and R4.3.3 were used for bibliometric analysis and visualization. For analysis 243 articles were included in this study. The number of publications on DUBs in ovarian cancer has gradually increased each year. China, the United States, and the United Kingdom are at the center of this field of research. The Johns Hopkins University, Genentech, and Roche Holding are the main research institutions. David Komander, Zhihua Liu, and Richard Roden are the top authors in this field. The top five journals with the largest publication volumes in this field are , , , , and . Keyword burst analysis identified five research areas: \"deubiquitinating enzyme,\" \"expression,\" \"activation,\" \"degradation,\" and \"ubiquitin.\" In addition, we summarized the expression profiles and biological roles of DUBs in ovarian cancer, highlighting their roles in tumor initiation, growth, chemoresistance, and metastasis. An overview of the research progress is provided in this study on DUBs in ovarian cancer over the last three decades. It offers insight into the most cited papers and authors, core journals, and identified new trends.

PurposeBolted joint is the most important connection method in aircraft composite/metal stacked connections due to its large load transfer capacity and high manufacturing reliability. Aircraft components are subjected to complex hybrid variable loads during service, and the mechanical properties of composite/metal bolted joint directly affect the overall safety of aircraft structures. Research on composite/metal bolted joint and their mechanical properties has also become a topic of general interests. This article reviews the current research status of aeronautical composite/metal bolted joint and its mechanical properties and looks forward to future research directions.Design/methodology/approachThis article reviews the research progress on static strength failure and fatigue failure of composite/metal bolted joint, focusing on exploring failure analysis and prediction methods from the perspective of the theoretical models. At the same time, the influence and correlation mechanism of hole-making quality and assembly accuracy on the mechanical properties of their connections are summarized from the hole-making processes and damage of composite/metal stacked structures.FindingsThe progressive damage analysis method can accurately analyze and predict the static strength failure of composite/metal stacked bolted joint structures by establishing a stress analysis model combined with composite material performance degradation schemes and failure criteria. The use of mature metal material fatigue cumulative damage models and composite material fatigue progressive damage analysis methods can effectively predict the fatigue of composite/metal bolted joints. The geometric errors such as aperture accuracy and holes perpendicularity have the most significant impact on the connection performance, and their mechanical responses mainly include ultimate strength, bearing stiffness, secondary bending effect and fatigue life.Research limitations/implicationsCurrent research on the theoretical prediction of the mechanical properties of composite/metal bolted joints is mainly based on ideal fits with no gaps or uniform gaps in the thickness direction, without considering the hole shape characteristics generated by stacked drilling. At the same time, the service performance evaluation of composite/metal stacked bolted joints structures is currently limited to static strength and fatigue failure tests of the sample-level components and needs to be improved and verified in higher complexity structures. At the same time, it also needs to be extended to the mechanical performance research under more complex forms of the external loads in more environments.Originality/valueThe mechanical performance of the connection structure directly affects the overall structural safety of the aircraft. Many scholars actively explore the theoretical prediction methods for static strength and fatigue failure of composite/metal bolted joints as well as the impact of hole-making accuracy on their mechanical properties. This article provides an original overview of the current research status of aeronautical composite/metal bolted joint and its mechanical properties, with a focus on exploring the failure analysis and prediction methods from the perspective of theoretical models for static strength and fatigue failure of composite/metal bolt joints and looks forward to future research directions.

Nature has evolved strategies to encode information within a single biopolymer to program biomolecular interactions with characteristic stoichiometry, orthogonality and reconfigurability. Nevertheless, synthetic approaches for programming molecular reactions or assembly generally rely on the use of multiple polymer chains (for example, patchy particles). Here we demonstrate a method for patterning colloidal gold nanoparticles with valence bond analogues using single-stranded DNA encoders containing polyadenine (polyA). By programming the order, length and sequence of each encoder with alternating polyA/non-polyA domains, we synthesize programmable atom-like nanoparticles (PANs) with n -valence that can be used to assemble a spectrum of low-coordination colloidal molecules with different composition, size, chirality and linearity. Moreover, by exploiting the reconfigurability of PANs, we demonstrate dynamic colloidal bond-breaking and bond-formation reactions, structural rearrangement and even the implementation of Boolean logic operations. This approach may be useful for generating responsive functional materials for distinct technological applications. Single-stranded DNA encoders containing polyadenine domains endow colloidal gold nanoparticles with programmable bond valence, orthogonality and reconfigurability, thus achieving post-synthetic control over colloidal structures.

Large volumes of product reviews generated by online users have important strategic value for product development. Prior studies often focus on the influence of reviews on customers' purchasing decisions through the word-of-mouth effect. However, little is known about how product developers respond to these reviews. This study adopts a big data analytical approach to investigate the impact of online customer reviews on customer agility and subsequently product performance. We develop a singular value decomposition-based semantic keyword similarity method to quantify customer agility using large-scale customer review texts and product release notes. Using a mobile app data set with over 3 million online reviews, our empirical study finds that review volume has a curvilinear relationship with customer agility. Furthermore, customer agility has a curvilinear relationship with product performance. Our study contributes to innovation literature by demonstrating the influence of firms' capability of utilizing online customer reviews and its impact on product performance. It also helps reconcile inconsistencies found in literature regarding the relationships among the three constructs.

DNA nanostructures are among the most fascinating self-assembled nanostructures in diverse areas of science and technology, because of their nanoscale precision in biomolecule and nanoparticle organization. The implementation of dynamic and spatial regulation in structural morphology and hierarchical assembly upon specific external stimuli will greatly expand their applications in biocomputation, clinical diagnosis, and cancer therapy. Recently, noncanonical nucleic acids, particularly DNA triplexes, i-motifs, and G-quadruplexes, have become powerful tools for biosensing and mechanical switching. Developments in incorporating stimuli-responsive noncanonical nucleic acids into DNA nanostructures provide a promising approach to regulating the spatial organization and hierarchical assembly of DNA nanostructures. In this review, we briefly introduce recent progress in constructing DNA nanostructures with dynamic regulation of the structural transformation and programmable assembly pathways at the nanometer scale by noncanonical nucleic acids and discuss their potential applications and challenges.DNA double helix exploiting Watson–Crick base-pairing lays the foundation of DNA nanotechnology. However, other forms of nucleic acids (e.g., triplex, i-motif, and G-quadruplex) exhibiting noncanonical base-base interactions bring about novel functionality. Here, we review the interplay of naturally occurring noncanonical nucleic acids and artificial DNA nanostructures in biomedical applications that have not been possible by duplex formation alone.

With the development of nanotechnology, significant progress has been made in the design, and manufacture of nanoparticles (NPs) for use in clinical treatments. Recent increases in our understanding of the central role of macrophages in the context of inflammation and cancer have reinvigorated interest in macrophages as drug targets. Macrophages play an integral role in maintaining the steady state of the immune system and are involved in cancer and inflammation processes. Thus, NPs tailored to accurately target macrophages have the potential to transform disease treatment. Herein, we first present a brief background information of NPs as drug carriers, including but not limited to the types of nanomaterials, their biological properties and their advantages in clinical application. Then, macrophage effector mechanisms and recent NPs-based strategies aimed at targeting macrophages by eliminating or re-educating macrophages in inflammation and cancer are summarized. Additionally, the development of nanocarriers targeting macrophages for disease diagnosis is also discussed. Finally, the significance of macrophage-targeting nanomedicine is highlighted, with the goal of facilitating future clinical translation.

Circulating tumor cells (CTCs) enable noninvasive liquid biopsy and identification of cancer. Various approaches exist for the capture and release of CTCs, including microfluidic methods and those involving magnetic beads or nanostructured solid interfaces. However, the concomitant cell damage and fragmentation that often occur during capture make it difficult to extensively characterize and analyze living CTCs. Here, we describe an aptamer-trigger-clamped hybridization chain reaction (atcHCR) method for the capture of CTCs by porous 3D DNA hydrogels. The 3D environment of the DNA networks minimizes cell damage, and the CTCs can subsequently be released for live-cell analysis. In this protocol, initiator DNAs with aptamer-toehold biblocks specifically bind to the epithelial cell adhesion molecule (EpCAM) on the surface of CTCs, which triggers the atcHCR and the formation of a DNA hydrogel. The DNA hydrogel cloaks the CTCs, facilitating quantification with minimal cell damage. This method can be used to quantitively identify as few as 10 MCF-7 cells in a 2-µL blood sample. Decloaking of tumor cells via gentle chemical stimulus (ATP) is used to release living tumor cells for subsequent cell culture and live-cell analysis. We also describe how to use the protocol to encapsulate and release cells of cancer cell lines, which can be used in preliminary experiments to model CTCs. The whole protocol takes ~2.5 d to complete, including downstream cell culture and analysis. An aptamer-trigger-clamped hybridization chain reaction (atcHCR) captures tumor cells in a protective, porous, 3D DNA hydrogel. Subsequent addition of ATP facilitates release of the tumor cells for use in downstream assays.

Patients with acute kidney injury (AKI) frequently require kidney transplantation and supportive therapies, such as rehydration and dialysis. Here, we show that radiolabelled DNA origami nanostructures (DONs) with rectangular, triangular and tubular shapes accumulate preferentially in the kidneys of healthy mice and mice with rhabdomyolysis-induced AKI, and that rectangular DONs have renal-protective properties, with efficacy similar to the antioxidant N -acetylcysteine—a clinically used drug that ameliorates contrast-induced AKI and protects kidney function from nephrotoxic agents. We evaluated the biodistribution of DONs non-invasively via positron emission tomography, and the efficacy of rectangular DONs in the treatment of AKI via dynamic positron emission tomography imaging with 68 Ga-EDTA, blood tests and kidney tissue staining. DNA-based nanostructures could become a source of therapeutic agents for the treatment of AKI and other renal diseases. DNA origami nanostructures with different shapes can accumulate preferentially in the kidney, with some being renal-protective, as shown in healthy mice and in a mouse model of acute kidney injury.

Type-B aflatoxins (AFB1 and AFB2) frequently contaminate food, especially nuts and fried figs, and seriously threaten human health; hence, it is necessary for the newly rapid and sensitive detection methods to prevent the consumption of potentially contaminated food. Here, a lateral flow aptasensor for the detection of type-B aflatoxins was developed. It is based on the use of fluorescent dye Cy5 as a label for the aptamer, and on the competition between type-B aflatoxins and the complementary DNA of the aptamer. This is the first time that the complementary strand of the aptamer has been used as the test line (T-line) to detect type-B aflatoxins. In addition, the truncated aptamer was used to improve the affinity with type-B aflatoxins in our study. Therefore, the lengths of aptamer and cDNA probe were optimized as key parameters for higher sensitivity. In addition, binding buffer and organic solvent were investigated. The results showed that the best pair for achieving improved sensitivity and accuracy in detecting AFB1 was formed by a shorter aptamer (32 bases) coupled with the probe complementary to the AFB1 binding region of the aptamer. Under the optimal experimental conditions, the test strip showed an excellent linear relationship in the range from 0.2 to 20 ng/mL with a limit of detection of 0.16 ng/mL. This aptamer-based strip was successfully applied to the determination of type-B aflatoxins in spiked and commercial peanuts, almonds, and dried figs, and the recoveries of the spiked samples were from 93.3%−112.0%. The aptamer-complementary strand-based lateral flow test strip is a potential alternative tool for the rapid and sensitive detection of type-B aflatoxins in nuts and dried figs. It is of help for monitoring aflatoxins to avoid the consumption of unsafe food.

Background Daqu , the saccharification, fermentation, and aroma-producing agents for Baijiu brewing, is prepared using a complex process. Aging is important for improving the quality of Daqu , but its impact has rarely been studied. This study investigated changes in the physicochemical properties, flavor compounds, and microbial communities during aging of Daqu with a roasted sesame-like flavor. Results The physicochemical properties changed continuously during aging to provide a high esterifying activity. Aging removed unpleasant flavor compounds and helped to stabilize the flavor compounds in mature Daqu . A high-throughput sequencing approach was used to analyze the changing composition of the microbial communities during aging. Aging helped to modify the microbial population to produce better Baijiu by eliminating low-abundance microbial communities and optimizing the proportion of predominant microbial communities. Nine genera of prokaryotic microbes formed the core microbiota in Daqu after aging. Regarding eukaryotic microbes, Zygomycota, the predominant community, increased in the first 2 months, then decreased in the third month of aging, while Ascomycota, the subdominant community, showed the opposite behavior. Absidia , Trichocomaceae_norank and Rhizopus were the predominant genera in the mature Daqu . Conclusions Significant correlations between microbiota and physicochemical properties or flavor compounds were observed, indicating that optimizing microbial communities is essential for aging Daqu . This study provides detailed information on aging during Daqu preparation.