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To improve the removal efficiency of rural domestic sewage, a two-stage multi-soil-layer sewage treatment system with an “aeration section + non-aeration section” was designed, and its treatment performance was observed under different influent loads and aeration intensities. The experiment ran for a total of 150 days, and the results showed that the two-stage multi-soil-layer (MSL) system could effectively reduce the effluent concentration of sewage to meet discharge standards. Under the operating conditions of an influent hydraulic load of 1000 L·m−2·d−1 and an air–water ratio of 4:1, the final effluent average concentrations of COD, NH3-N, TN, and TP were 106.5 mg·L−1, 7.4 mg·L−1, 13.9 mg·L−1, 0.12 mg·L−1, and 18.6 mg·L−1, respectively, with average removal rates of 85.3%, 82%, 72.5%, 96%, and 85%. A longer hydraulic retention time and ideal anoxic conditions were ensured by designing a certain effluent height in the system. Adding aeration to the system allowed for a synchronous nitrification–denitrification reaction under reasonable influent loads, ultimately enabling the effluent to meet discharge standards.

Background Classifying and characterizing pulmonary lesions are critical for clinical decision-making process to identify optimal therapeutic strategies. The purpose of this study was to develop and validate a radiomics nomogram for distinguishing between benign and malignant pulmonary lesions based on robust features derived from diffusion images. Material and methods The study was conducted in two phases. In the first phase, we prospectively collected 30 patients with pulmonary nodule/mass who underwent twice EPI-DWI scans. The robustness of features between the two scans was evaluated using the concordance correlation coefficient (CCC) and dynamic range (DR). In the second phase, 139 patients who underwent pulmonary DWI were randomly divided into training and test sets in a 7:3 ratio. Maximum relevance minimum redundancy, least absolute shrinkage and selection operator, and logistic regression were used for feature selection and construction of radiomics signatures. Nomograms were established incorporating clinical features, radiomics signatures, and ADC (0, 800) . The diagnostic efficiency of different models was evaluated using the area under the curve (AUC) and decision curve analysis. Results Among the features extracted from DWI and ADC images, 42.7% and 37.4% were stable (both CCC and DR ≥ 0.85). The AUCs for distinguishing pulmonary lesions in the test set for clinical model, ADC, ADC radiomics signatures, and DWI radiomics signatures were 0.694, 0.802, 0.885, and 0.767, respectively. The nomogram exhibited the best differentiation performance (AUC = 0.923). The decision curve showed that the nomogram consistently outperformed ADC value and clinical model in lesion differentiation. Conclusion Our study demonstrates the robustness of radiomics features derived from lung DWI. The ADC radiomics nomogram shows superior clinical net benefits compared to conventional clinical models or ADC values alone in distinguishing solitary pulmonary lesions, offering a promising tool for noninvasive, precision diagnosis in lung cancer.

With the continuous development of ecological city construction under the concept of “ecological civilization”, the creation of ecologically livable cities has become more and more important. Since urban livability involves a wide range of social life, the establishment of urban livability index evaluation system can help analyze urban livability and residents’ quality of life, and then optimize and control urban spatial structure to promote high-quality urban development. This study takes Wuhan, Hubei Province, as an example, and establishes a comprehensive index evaluation system based on 12 indicators in three criteria levels: economic, social, and environmental, and calculates a comprehensive score of urban livability for each district in Wuhan City, and conducts a comprehensive evaluation of the 13 administrative districts under Wuhan City. Based on the final scores, we analyze the livability and friendliness of each district in Wuhan, analyze the spatial pattern of the results, discuss the strengths and weaknesses of the livability of each district in Wuhan, make scientific reference suggestions to promote the coordinated development of each district, guide the rational allocation of resources, and provide a reference for decision-making to promote the livable communities in each district in Wuhan.

The problems of large deformations, failures, and fractures that agricultural tillage tools may encounter during the cultivation process has long been a concern in the field of agricultural machinery design and manufacturing. It is important to establish a more accurate numerical model to effectively predict tools’ plastic deformation failures and ductile fracture failures. This research develops a numerical model for predicting the plastic deformation failure and ductile fracture failure of agricultural tillage tools using the smoothed particle hydrodynamics (SPH) method and the Johnson–Cook constitutive model. The model uses the Drucker–Prager criterion to describe the elastic–plastic constitutive behavior of the soil, the von Mises criterion to describe the Johnson–Cook constitutive model of the tool, and the coupling condition with the Lennard-Jones repulsive force to describe the interaction between the tool and soil. The numerical results show that the proposed model can effectively simulate the interaction between the tool and soil, as well as the tool’s plastic deformation failure and ductile fracture failure during the agricultural cultivation process. It can also predict the variation trend of the cutting force of the tool. This helps to provide a new approach for the numerical simulation of such problems.

The occurrence of slope instability disasters seriously endangers the safety of people’s lives and property in China. Therefore, it is essential to study the slope instability process and the interaction between soil and retaining walls. In this paper, the smoothed-particle hydrodynamics (SPH) method, based on the elastoplastic constitutive model of rock and soil, was used to simulate the entire process of slope instability and the interaction between soil and retaining walls. The model, based on the classical elastic–plastic theory, includes linear elastic deformation and plastic deformation following the non-associated flow rule under the Drucker–Prager (DP) yield criterion. By considering the plastic characteristics of geotechnical materials, this method can accurately simulate the slope movement process. The model was established, calculated, and compared with a slope example, thus verifying its feasibility. Furthermore, the motion response of the retaining wall under different conditions was studied, which provides a new numerical simulation platform for the stability checking of the retaining wall and motion analysis after the interaction between the retaining wall and slope soil.

The present study was conducted to evaluate the impact of dietary fully oxidised β-carotene (OxBC, C40H60O15) supplementation during the perinatal period on immune status and productivity in a sow model. At day 85 of pregnancy, 150 sows were allocated to one of three dietary treatments with fifty sows per treatment. The three experimental diets were supplemented with 0, 4 or 8 mg/kg OxBC in the basal diet. The feeding trial was conducted from gestation day 85 until day 21 of lactation. Dietary OxBC supplementation greatly enhanced colostrum IgM, IgA and IgG levels, and the IgM and IgG content of 14-d milk. Dietary OxBC supplementation decreased the TNF-α and IL-8 levels in colostrum, as well as the TNF-α and IL-18 levels in 14-d milk. There was also a tendency towards an increase in the soluble CD14 level in 14-d milk. Although dietary treatments did not affect average daily feed intake nor backfat thickness loss during lactation, dietary OxBC supplementation tended to enhance litter weight and individual piglet weight at weaning. There was a trend towards increased lactose concentration in 14-d milk with increasing dietary OxBC. It is concluded that dietary supplementation with OxBC during the perinatal period enhances the lactose concentration of sow milk and the immune status of sows, which is reflected by improved cytokine status and immunoglobulin concentrations in colostrum and milk, and thus tending to increase litter weight and individual piglet weight at weaning. The results also provide a scientific nutritional reference for perinatal mothers due to the biological similarity between pigs and humans.

Endometrioid endometrial carcinoma (EEC) is the most dominant subtype of endometrial cancer. Aberrant transcriptional regulation has been implicated in EEC. Herein, we characterized mRNA and miRNA transcriptomes by RNA sequencing in EEC to investigate potential molecular mechanisms underlying the pathogenesis. Total mRNA and small RNA were simultaneously sequenced by next generation sequencing technology for 3 pairs of stage I EEC and adjacent non-tumorous tissues. On average, 52,716,765 pair-end 100 bp mRNA reads and 1,669,602 single-end 50 bp miRNA reads were generated. Further analysis indicated that 7 miRNAs and 320 corresponding target genes were differentially expressed in the three stage I EEC patients. Six of all the seven differentially expressed miRNAs were targeting on eleven differentially expressed genes in the cell cycle pathway. Real-time quantitative PCR in sequencing samples and other independent 21 pairs of samples validated the miRNA-mRNA differential co-expression, which were involved in cell cycle pathway, in the stage I EEC. Thus, we confirmed the involvement of hsa-let-7c-5p and hsa-miR-99a-3p in EEC and firstly found dysregulation of hsa-miR-196a-5p, hsa-miR-328-3p, hsa-miR-337-3p, and hsa-miR-181c-3p in EEC. Moreover, synergistic regulations among these miRNAs were detected. Transcript sequence variants such as single nucleotide variant (SNV) and short insertions and deletions (Indels) were also characterized. Our results provide insights on dysregulated miRNA-mRNA co-expression and valuable resources on transcript variation in stage I EEC, which implies the new molecular mechanisms that underlying pathogenesis of stage I EEC and supplies opportunity for further in depth investigations.

Endometrial carcinoma is one of the most common malignancies in the female reproductive system. It is well-known that estrogen plays an important role in the pathogenesis of endometrioid endometrial carcinoma (EEC), and induces the cancer suppressor gene PTEN deletion. However, how estrogen affects PTEN expression remains unknown. In the present study, we found in 40 EEC specimens, miR-200c level was higher in most cancer areas than that in the adjacent normal endometrium, while PTEN and PTENP1 were lower. Moreover, the expression of PTEN/PTENP1 and miR-200c also showed a converse relationship in EEC cell lines. In addition, we demonstrated that miR-200c bound directly to PTEN and PTENP1, and PTENP1 could reverse miR-200c inhibition function to PTEN using a dual-luciferase reporter and RNA binding protein immunoprecipitation (RIP) assays. Next, 17β-estradiol (E2) treatment could improve miR-200c and drop the PTEN level, which caused a consequential increase of the phospho-PI3K-AKT pathway genes. When we stably knocked down estrogen receptor α (ERα) expression in the EEC cell line, the effects of E2 on miR-200c and PTEN declined. In addition, it was demonstrated that E2 might modulate cell proliferation, migration and invasion relying on the expression of miR-200c. Taken together, it can be concluded that estrogen improves the miR-200c level by combining with ER, PTENP1 and PTEN could be inhibited by miR-200c, and then activate the PI3K-AKT pathway. This work provided a new mechanism of EEC development and a new potential therapeutic target.

This study aimed to evaluate the effects of dietary supplementation of fermentation product (SCFP) during late pregnancy and lactation on sow productivity, colostrum and milk composition, and antioxidant status of sows in a subtropical climate. The study was a 2 × 2 factorial treatment design where the first factor was environmental THI level [Low THI (08:00-19:00: 70.76 ± 0.45, 19:00-08:00: 67.91 ± 0.18, L-THI) or High THI (08:00-19:00: 75.14 ± 0.98, 19:00-08:00: 68.35 ± 0.18, H-THI], and the second factor was dietary treatment (supplemented with or without 3 kg/t SCFP). A total of 120 sows were randomly allotted to the four treatments ( = 30). The feeding trial was conducted from 85-days post-breeding until 21-days post-partum. Compared with L-THI group, sows from H-THI group had lesser individual piglet birth weight, individual piglet weight at weaning, preweaning average daily gain of piglets, average daily feed intake of sows during lactation, and protein percentage in 14-days milk. Additionally, sows from H-THI group had lesser antioxidant status, indicated by lesser serum total antioxidant capacity (T-AOC), and superoxide dismutase (SOD) activity at parturition; lesser serum T-AOC and glutathione peroxidase (GSH-Px) activity at 14-days post-partum, as well as lesser SOD activity in colostrum. Compared with sows fed the control diet, sows fed the SCFP diet had greater number of piglets weaned, litter weight at weaning, and preweaning average daily gain of piglets. Moreover, sows fed the SCFP diet had improved antioxidant status as indicated by higher serum T-AOC at parturition, and lesser malondialdehyde (MDA) content in colostrum and 21-days milk. In conclusion, H-THI negatively affected the productivity, milk composition, antioxidant status, and lactation feed intake of sows. Dietary supplementation of SCFP partially alleviated the adverse effects of H-THI, by improving lactation performance and antioxidant status of sows without influencing reproductive performance and colostrum and milk composition in a subtropical climate.

Lactose plays a crucial role in controlling milk volume by inducing water toward into the mammary secretory vesicles from the mammary epithelial cell cytoplasm, thereby maintaining osmolality. In current study, we determined the expression of several lactose synthesis related genes, including glucose transporters (glucose transporter 1, glucose transporter 8, sodium-glucose cotransporter 1, sodium-glucose cotransporter 3, and sodium-glucose cotransporter 5), lactose synthases (α-lactalbumin and β1,4-galactosyltransferase), and hexokinases (hexokinase-1 and hexokinase-2) in sow mammary gland tissue at day 17 before delivery, on the 1st day of lactation and at peak lactation. The data showed that glucose transporter 1 was the dominant glucose transporter within sow mammary gland and that expression of each glucose transporter 1, sodium-glucose cotransporter 1, hexokinase-1, hexokinase-2, α-lactalbumin, and β1,4-galactosyltransferase were increased ( p  < 0.05) when the sows transited from late pregnancy to peak lactation. AKT1 over-expressed mammary epithelial cells were then constructed, and the results indicated that AKT1 increases ( p  < 0.01) the expression of hexokinase-1 and glucose transporter 1. In summary, lactose synthesis was significantly elevated with the increase of milk production and AKT1 could positively regulate lactose synthesis.