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Objective This study aimed to observe the impact of Tthoracic paravertebral nerve blockade(TPVB) at left T7 level on the α7nAChR-dependent cholinergic anti-inflammatory pathway in patients undergoing thoracoscopic lobectomy. Methods Scheduled thoracoscopic lung surgery patients at the First Affiliated Hospital of Nanchang University from August to September 2023 were divided into two groups according to the surgical site. The experimental group underwent left T7 paravertebral nerve blockade (LTPVB group), while the control group underwent right T7 paravertebral nerve blockade (RTPVB group). Relevant clinical data were collected, and Doppler ultrasound was used to measure the resistive index (RI) of the splenic artery before and after blockade. Additionally, perioperative α7nAChR levels and the expression levels of the inflammatory factors IL-1β, IL-6, and TNF-α were determined. Results There were no significant differences in general conditions, perioperative blood pressure, heart rate, or pain VAS scores between the two groups ( p  > 0.05). Splenic Doppler ultrasound showed that compared to before blockade, the RI of the splenic artery in the LTPVB group significantly decreased ( p  < 0.05). The α7nAChR levels at 12 h and 24 h postoperatively were significantly increased ( p  < 0.05) in both groups, and the levels of IL-1β, IL-6, and TNF-α gradually increased over time in both groups. However, the levels were significantly lower in the LTPVB group compared to the RTPVB group at 12 h and 24 h postoperatively ( p  < 0.05). Conclusion TPVB at left T7 can activate the α7nAChR-dependent cholinergic anti-inflammatory pathway, thereby alleviating the postoperative inflammatory response in patients undergoing thoracoscopic lobectomy.

Objective To investigate the effect of lidocaine on the expression of voltage-dependent anion channel 1 (VDAC1) in breast invasive carcinoma (BRCA) and its impact on the apoptosis of breast cancer cells. Methods We collected clinical data from patients with invasive breast cancer from 2010 to 2020 in the First affiliated hospital of Nanchang University, evaluated the prognostic value of VDAC1 gene expression in breast cancer, and detected the expression of VDAC1 protein in breast cancer tissues and paracancerous tissues by immunohistochemical staining of paraffin sections. Also, we cultured breast cancer cells (MCF-7) to observe the effect of lidocaine on the apoptosis of MCF-7 cells. Results Analysis of clinical data and gene expression data of BRCA patients showed VDAC1 was a differentially expressed gene in BRCA, VDAC1 may be of great significance for the diagnosis and prognosis of BRCA patients. Administration of lidocaine 3 mM significantly decreased VDAC1 expression, the expression of protein Bcl-2 was significantly decreased ( p  < 0.05), and the expression of p53 increased significantly ( p  < 0.05). Lidocaine inhibited the proliferation of MCF-7 breast cancer cells, increased the percentage of G2 / M phase cells and apoptosis. Conclusion Lidocaine may inhibit the activity of breast cancer cells by inhibiting the expression of VDAC1, increasing the apoptosis in breast cancer cells.

Transitional separating flow induced by a rectangular plate subjected to uniform incoming flow at Reynolds number (based on the incoming velocity and half plate height) 2000 is investigated using direct numerical simulation. The objective is to unveil the long-lasting mystery of low-frequency flapping motion (FM) in flow separation. At a fixed streamwise-vertical plane or from the perspective of previous experimental studies using pointwise or planar measurements, FM manifests as a low-frequency periodic switching between low and high velocities covering the entire separation bubble. The results indicate that in three-dimensional space, FM reflects an intricate evolution of streamwise elongated streaky structures under the influence of separated shear layer and mean flow reversal. The FM is an absolute instability, and is initiated through a lift-up mechanism boosted by mean flow deceleration near the crest of the separating streamline. At this particular location, the shear bends the vortex filament abruptly, so that one end is vertically struck into the first half of the separation bubble, whereas the other end is extended in the streamwise direction in the second half of the separation bubble. These two ends of vortex filament are mutually sustained and also stretched by the vertical acceleration and streamwise acceleration in the first and second halves of the separation bubble, respectively. This process periodically switches the low-velocity (or high-velocity) streaky structure to a high-velocity (or low-velocity) streaky structure encompassing the entire separation bubble, and thus flaps the separated shear layer up and down in the vertical direction. A ‘large vortex’ shedding manifests when the streaky structure switches signs. This large vortex is fundamentally different from the spanwise vortex shedding residing in the shear layer originated from the Kelvin–Helmholtz instability and successive vortex amalgamation. It is also believed that the three-dimensional evolution of streaky structures in the form of FM is applicable for both geometry- and pressure-induced separating flows.

Turbulent separation bubbles over and behind a two-dimensional forward–backward-facing step submerged in a deep turbulent boundary layer are investigated using a time-resolved particle image velocimetry. The Reynolds number based on the step height and free-stream velocity is 12 300, and the ratio of the streamwise length to the height of the step is 2.36. The upstream turbulent boundary layer thickness is 4.8 times the step height to ensure a strong interaction of the upstream turbulence structures with the separated shear layers over and behind the step. The velocity measurements were performed in streamwise–vertical planes at the channel mid-span and streamwise–spanwise planes at various vertical distances from the wall. The unsteady characteristics of the separation bubbles and their associated turbulence structures are studied using a variety of techniques including linear stochastic estimation, proper orthogonal decomposition and variable-interval time averaging. The results indicate that the low-frequency flapping motion of the separation bubble over the step is induced by the oncoming large-scale alternating low- and high-velocity streaky structures. Dual separation bubbles appear periodically over the step at a higher frequency than the flapping motion, and are attributed to the inherent instability in the rear part of the mean separation bubble. The separation bubble behind the step exhibits a flapping motion at the same frequency as the separation bubble over the step, but with a distinct phase delay. At instances when an enlarged separation bubble is formed in front of the step, a pair of vertical counter-rotating vortices is formed in the immediate vicinity of the leading edge.

The effects of large-scale motion (LSM) on the spatio-temporal dynamics of separated shear layers induced by a forward-facing step submerged in a thick turbulent boundary layer (TBL) are investigated using a time-resolved particle image velocimetry. The Reynolds number based on the free-stream velocity and step height was 13 200. The oncoming TBL was developed over a cube-roughened surface and the thickness was 6.5 times the step height. The step height was chosen to coincide with the elevation where the dominant frequency of streamwise fluctuating velocity in the TBL occurred. At this elevation, the local turbulence intensity was 14.5 %. Distinct regions of elevated Reynolds stresses were observed upstream and downstream of the leading edge of the step. The unsteady dynamics of the separation bubbles upstream and downstream of the step was investigated using the reverse flow area. Both separation bubbles exhibit low-frequency flapping motion, and the dominant frequency of the downstream separation bubble is identical to the dominant frequency of the streamwise fluctuating velocity in the oncoming TBL at the step height. As the low-velocity region of LSM passes over the step, the downstream separation bubble is enlarged and subsequently undergoes a high-frequency oscillation. Turbulence motions were partitioned into low-, medium- and high-frequency regimes based on spectral analysis of the Reynolds stresses. The contributions from these partitioned turbulence motions are used to elucidate the effects of LSM on the elevated Reynolds stresses in the shear layers upstream and downstream of the step.

The spatio-temporal characteristics of turbulent separations beneath semi-submerged bluff bodies with different undersurface roughness conditions are studied using a time-resolved particle image velocimetry. The Reynolds number based on the free-stream velocity and submergence depth was fixed to 14 400. Three different undersurface conditions – smooth, sandpaper roughness and cube roughness – were examined. The results showed that wall roughness reduces the mean reattachment length, and suppresses the Reynolds stresses in the second half of the mean separation bubble. The Kelvin–Helmholtz instability is observed at the leading edge of the smooth bluff body, but is bypassed in the rough cases. In the first half of the mean separation bubble, the frequencies in the separated shear layer migrate to lower values in a discrete manner through the vortex pairing mechanism. Consequently, multiple vortex shedding motions at different frequencies are nested in the separated shear layer, and the cores of shed vortices are aligned near the isopleth of free-stream velocity. The shed vortex is accompanied with multiple vortices along the edge of mean flow reversal in the upstream locations. These vortices are influenced significantly by wall roughness. A low-frequency flapping motion manifests as enlargement/shrinkage of reverse flow areas in the first half of the mean separation bubble. The frequencies of flapping motion in the smooth and sandpaper cases are similar, but are relatively lower than that in the cube roughness case. This flapping motion is associated with an extremely large vortex shed from the mean reattachment point to the free-stream region.

The spatio-temporal dynamics of separation bubbles induced by surface-mounted bluff bodies with different spanwise widths and submerged in a thick turbulent boundary layer is experimentally investigated. The streamwise extent of the bluff bodies is fixed at 2.36 body heights and the spanwise aspect ratio ($AR$), defined as the ratio between the width and height, is increased from 1 to 20. The thickness of the upstream turbulent boundary layer is 4.8 body heights, and the dimensionless shear and turbulence intensity evaluated at the body height are 0.23 % and 15.8 %, respectively, while the Reynolds number based on the body height and upstream free-stream velocity is 12 300. For these upstream conditions and limited streamwise extent of the bluff bodies, two distinct and strongly interacting separation bubbles are formed over and behind the bluff bodies. A time-resolved particle image velocimetry is used to simultaneously measure the velocity field within these separation bubbles. Based on the dynamics of the mean separation bubbles over and behind the bluff bodies, the flow fields are categorized into three-dimensional, transitional and two-dimensional regimes. The results indicate that the low-frequency flapping motions of the separation bubble on top of the bluff body with$\\mathit{AR}=1$are primarily influenced by the vortex shedding motion, while those with larger aspect ratios are modulated by the large-scale streamwise elongated structures embedded in the oncoming turbulent boundary layer. For$\\mathit{AR}=1$and 20, the flapping motions in the wake region are strongly influenced by those on top of the bluff bodies but with a time delay that is dependent on the$AR$. Moreover, an expansion of the separation bubble on the top surface tends to lead to an expansion and contraction of separation bubbles in the wake of$\\mathit{AR}=20$and 1, respectively. As for the transitional case of$\\mathit{AR}=8$, the separation bubbles over and behind the body are in phase over a wide range of time difference. The dynamics of the shear layer in the wake region of the transitional case is remarkably more complex than the limiting two-dimensional and three-dimensional configurations.

This study aimed to observe the impact of Tthoracic paravertebral nerve blockade(TPVB) at left T7 level on the [alpha]7nAChR-dependent cholinergic anti-inflammatory pathway in patients undergoing thoracoscopic lobectomy. Scheduled thoracoscopic lung surgery patients at the First Affiliated Hospital of Nanchang University from August to September 2023 were divided into two groups according to the surgical site. The experimental group underwent left T7 paravertebral nerve blockade (LTPVB group), while the control group underwent right T7 paravertebral nerve blockade (RTPVB group). Relevant clinical data were collected, and Doppler ultrasound was used to measure the resistive index (RI) of the splenic artery before and after blockade. Additionally, perioperative [alpha]7nAChR levels and the expression levels of the inflammatory factors IL-1[beta], IL-6, and TNF-[alpha] were determined. There were no significant differences in general conditions, perioperative blood pressure, heart rate, or pain VAS scores between the two groups (p > 0.05). Splenic Doppler ultrasound showed that compared to before blockade, the RI of the splenic artery in the LTPVB group significantly decreased (p < 0.05). The [alpha]7nAChR levels at 12 h and 24 h postoperatively were significantly increased (p < 0.05) in both groups, and the levels of IL-1[beta], IL-6, and TNF-[alpha] gradually increased over time in both groups. However, the levels were significantly lower in the LTPVB group compared to the RTPVB group at 12 h and 24 h postoperatively (p < 0.05). TPVB at left T7 can activate the [alpha]7nAChR-dependent cholinergic anti-inflammatory pathway, thereby alleviating the postoperative inflammatory response in patients undergoing thoracoscopic lobectomy.

Separating and reattaching turbulent flows induced by a forward-facing step subjected to an incoming fully developed turbulent channel flow are studied using direct numerical simulation. The step height is one quarter of the channel height, and the Reynolds number based on friction velocity and half-channel height at the inlet is 180. The three-dimensional spatio-temporal characteristics of separation bubbles upstream and downstream of the step are analysed with particular attention to the effects of impinging hairpin structures and the topology of principal stretching. Immediately upstream of the step, the fluctuating vorticity parallel to the mean streamlines is significant. On the frontal surface of the step, strong spanwise skin friction appears in the form of alternating positive and negative values in vertical strips. Over the step, the principal stretching switches orientation along a curve emanating from the leading edge, which is termed the principal stretching line (PSL). The reverse flows upstream and downstream of the step possess dominant and harmonic frequencies that mirror those of the incoming flow. As a hairpin structure leans over the step, the associated vorticity is deformed by the principal stretching. Specifically, PSL marks the lower bound of the deformed hairpin legs, and an opposite-signed pair of counter-rotating quasi-streamwise vortices are induced near the top surface of the step. Consequently, the separation bubbles upstream of and over the step are enlarged and suppressed, respectively. For a sufficiently strong hairpin structure interacting with the step, an open-type separation occurs upstream of the step, while dual separation bubbles appear over the step.

Nanothermometers enable the detection of temperature changes at the microscopic scale, which is crucial for elucidating biological mechanisms and guiding treatment strategies. However, temperature monitoring of micron-scale structures in vivo using luminescent nanothermometers remains challenging, primarily due to the severe scattering effect of biological tissue that compromises the imaging resolution. Herein, a lanthanide luminescence nanothermometer with a working wavelength beyond 1500 nm is developed to achieve high-resolution temperature imaging in vivo. The energy transfer between lanthanide ions (Er 3+ and Yb 3+ ) and H 2 O molecules, called the environment quenching assisted downshifting process, is utilized to establish temperature-sensitive emissions at 1550 and 980 nm. Using an optimized thin active shell doped with Yb 3+ ions, the nanothermometer’s thermal sensitivity and the 1550 nm emission intensity are enhanced by modulating the environment quenching assisted downshifting process. Consequently, minimally invasive temperature imaging of the cerebrovascular system in mice with an imaging resolution of nearly 200 μm is achieved using the nanothermometer. This work points to a method for high-resolution temperature imaging of micron-level structures in vivo, potentially giving insights into research in temperature sensing, disease diagnosis, and treatment development. The strong scattering of biological tissue causes challenges when monitoring temperature changes at the microscale. Here, the authors propose a nanothermometer based on lanthanide luminescence, enabling minimally invasive imaging of the cerebrovascular system of mice at nearly 200 μm resolution.