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Solute carrier family 25 (SLC25) encodes transport proteins at the inner mitochondrial membrane and functions as carriers for metabolites. Although SLC25 genetic variants correlate with human metabolic diseases, their roles in colon cancer remain unknown. Cases of colon cancer were retrieved from The Cancer Genome Atlas, and the transcriptionally differentially expressed members (DEMs) of SLC25 were identified. DNA level alterations, clinicopathological characteristics, and clinical survival were also investigated. A risk score model based on the DEMs was constructed to further evaluate their prognostic values in a clinical setting. The results were preliminarily validated using bioinformatic analysis of datasets from the Gene Expression Omnibus, immunohistochemical evaluations in clinical specimens, and functional experiments in colon cancer-derived cell lines. Thirty-seven DEMs were identified among 53 members of SLC25. Eight of 37 DEMs were introduced into a risk score model using integrated LASSO regression and multivariate Cox regression. Validated by GSE395282 and GSE175356, DEMs with high-risk scores were associated with the phenotypes of increasing tumor immune infiltration and decreasing glycolysis and apoptosis contents. SLC25A5 was downregulated in cancer, and its upregulation was related to better overall survival in patients from public datasets and in clinical cases. High SLC25A5 expression was an independent prognostic factor for 79 patients after surgical treatment. A negative correlation between CD8 and SLC25A5 was determined in specimens from 106 patients with advanced colon cancer. SLC25A5 attenuated cell proliferation, upregulated the expression of programmed cell death-related signatures, and exerted its biological function by inhibiting the MAPK signaling pathway. Our study reveals that mitochondrial SLC25 has prognostic value in patients with colon cancer. The bioinformatic analyses by following verification in situ and in vitro provide direction for further functional and mechanistic studies on the identified member of SLC25.

Although checkpoint blockade is a promising approach for the treatment of hepatocellular carcinoma (HCC), subsets of patients expected to show a response have not been established. As T cell-mediated tumor killing (TTK) is the fundamental principle of immune checkpoint inhibitor therapy, we established subtypes based on genes related to the sensitivity to TKK and evaluated their prognostic value for HCC immunotherapies. Genes regulating the sensitivity of tumor cells to T cell-mediated killing (referred to as GSTTKs) showing differential expression in HCC and correlations with prognosis were identified by high-throughput screening assays. Unsupervised clustering was applied to classify patients with HCC into subtypes based on the GSTTKs. The tumor microenvironment, metabolic properties, and genetic variation were compared among the subgroups. A scoring algorithm based on the prognostic GSTTKs, referred to as the TCscore, was developed, and its clinical and predictive value for the response to immunotherapy were evaluated. In total, 18 out of 641 GSTTKs simultaneously showed differential expression in HCC and were correlated with prognosis. Based on the 18 GSTTKs, patients were clustered into two subgroups, which reflected distinct TTK patterns in HCC. Tumor-infiltrating immune cells, immune-related gene expression, glycolipid metabolism, somatic mutations, and signaling pathways differed between the two subgroups. The TCscore effectively distinguished between populations with different responses to chemotherapeutics or immunotherapy and overall survival. TTK patterns played a nonnegligible role in formation of TME diversity and metabolic complexity. Evaluating the TTK patterns of individual tumor will contribute to enhancing our cognition of TME characterization, reflects differences in the functionality of T cells in HCC and guiding more effective therapy strategies.

Aflatoxin B1 (AFB1) is a ubiquitous mycotoxin produced by toxicogenic Aspergillus species. AFB1 has been reported to cause serious adverse health effects, such as cancers and abnormal development and reproduction, in animals and humans. AFB1 is also a potent genotoxic mutagen that causes DNA damage in vitro and in vivo. However, the link between DNA damage and abnormal development and reproduction is unclear. To address this issue, we examined the DNA damage, germline apoptosis, growth, and reproductive toxicity following exposure to AFB1, using Caenorhabditis elegans as a study model. Results found that AFB1 induced DNA damage and germline apoptosis, and significantly inhibited growth and reproduction of the nematodes in a concentration-dependent manner. Exposure to AFB1 inhibited growth or reproduction more potently in the DNA repair-deficient xpa-1 nematodes than the wild-type N2 strain. According to the relative expression level of pathway-related genes measured by real-time PCR, the DNA damage response (DDR) pathway was found to be associated with AFB1-induced germline apoptosis, which further played an essential role in the dysfunction of growth and reproduction in C. elegans.

Morus, a plant genus from the family of Moraceae, most plants of which are used as traditional medicines in Asian counties. The root barks of Morus plants are normally called as Sang-Bai-Pi (SBP) in Chinese and used for the treatment of inflammatory and respiratory diseases. Decades of research on phytochemistry of SBP have led to the identification of various compounds, such as Diels-Alder-type adducts, flavonoids, benzofurans, stilbenes, polyhydroxylated alkaloids, etc. These compounds have showed a wide range of bioactive features including anti-inflammatory, anti-oxidative, anti-microbic, etc. This review focus on the bioactive compounds and their pharmacological effects of SBP which will help us fully understand the effective substances of SBP, and pave our way to further explore medicinal uses of SBP and comprehensive utilization of Morus species.

Developmental craniofacial deformity often has serious infection on the patient's chewing, language ability, swallowing, breathing and other related functions. Currently, internal and linear Distraction Osteogenesis (DO) are main surgery solutions of bone transportation and recovery in clinic. In this paper, we present a computer system called LinDOS for maxillofacial system reconstruction and visualization, maxillofacial tissues stress distribution analysis, as well as linear distraction osteogenesis simulation. Both design architecture and the function modules of the system are described in detail. The system can be employed as an assistant tool of maxillofacial preoperative planning and postoperative prediction for maxillofacial surgeons.

Sigma factors of the extracytoplasmic function (ECF) subfamily are important regulators of stress responses in bacteria. This work described the characterization of ECF sigma factor SigT in Streptomyces coelicolor. We found the absence of sigT almost abolished the production of the antibiotics actinorhodin (Act) under nitrogen stress. Under nitrogen-limited conditions, significantly reduced Act production and linked actII-ORF4 transcription with respect to wild type were observed in the sigT-null mutant. Using reporter (xylE) fusion to sigT promoter, we demonstrated that sigT was induced by nitrogen limitation in a SigT-dependent manner. Transcriptional analyses showed that SigT controlled the expression of relA, the ppGpp synthetase gene, and consequently affected the Act production upon nitrogen starvation. Co-transcription analysis revealed that sigT was co-transcribed with rstB (gene upstream of sigT) but not with rstA (gene downstream of sigT). Phenotypic and transcriptional results suggested RstA may modulate the activity of SigT positively.[PUBLICATION ABSTRACT]

The negative factors of the colored asphalt mixture extremely correlate to its high-temperature behavior. With the Grey Relation Analysis, this paper makes a thorough analysis of the affective factors on the high-temperature behavior of the colored asphalt mixture, the result of which shows that the affective factors can be classified as the softening point of colored asphalt and the aggregate ratio of mixture. The research findings will provide a beneficial reference for the design of colored asphalt mixture.

This article adopts resistance method measuring saturation, and do research about the impact of polymer resistance - saturation curve measurement. Results showed that in the resistance method for detection of residual oil distribution, there are significant effects of the experiment of water salinity on measuring the resistance and standard curve. When the polymer water ionic concentration is small, resistances of polymers are double impacted by the salinity of formation water and the polymer concentration. Experiments show that as the polymer concentration increased, the resistance value decreases. In view of the configuration of CNOOC formation water polymer, concentration has no effect on resistance of basic, the influence of polymer for measuring resistance value and relation curve is mainly depended on formation water salinity, and consistent with the influence of formation water salinity.

Advancement of biomedical applications of carbonaceous nanomaterials is hampered by their biopersistence and pro-inflammatory action in vivo. Here, we used myeloperoxidase knockout B6.129X1-MPO (MPO k/o) mice and showed that oxidation and clearance of single walled carbon nanotubes (SWCNT) from the lungs of these animals after pharyngeal aspiration was markedly less effective whereas the inflammatory response was more robust than in wild-type C57Bl/6 mice. Our results provide direct evidence for the participation of MPO - one of the key-orchestrators of inflammatory response - in the in vivo pulmonary oxidative biodegradation of SWCNT and suggest new ways to control the biopersistence of nanomaterials through genetic or pharmacological manipulations.