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Objective We conducted a systematic review and meta-analysis to clarify the effects of different exercise modes (resistance training [RT], whole body vibration training [WBVT], and mixed training [MT, resistance training combined with other exercises such as balance, endurance and aerobic training]) on muscle strength (knee extension strength [KES]) and physical performance (Timed Up and Go [TUG], gait speed [GS] and the Chair Stand [CS]) in older people with sarcopenia. Method All studies published from January 2010 to March 2021 on the effects of exercise training in older people with sarcopenia were retrieved from 6 electronic databases: Pubmed, Cochrane Library, Embase, Web of Science, the China National Knowledge Infrastructure (CNKI), and Wanfang Database. Two researchers independently extracted and evaluated studies that met inclusion and exclusion criteria. Pooled analyses for pre- and post- outcome measurements were performed using Review Manager 5.4 with standardized mean differences (SMDs) and fixed-effect models. Result Twenty-six studies (25 randomized controlled trails [RCTs] and one non-randomized controlled trail) were included in this study with 1191 older people with sarcopenia (mean age 60.6 ± 2.3 to 89.5 ± 4.4). Compared with a control group, RT and MT significantly improved KES (RT, SMD = 1.36, 95% confidence intervals [95% CI]: 0.71 to 2.02, p  < 0.0001, I 2  = 72%; MT, SMD = 0.62, 95% CI: 0.29 to 0.95, p  = 0.0002, I 2  = 56%) and GS (RT, SMD = 2.01, 95% CI: 1.04 to 2.97, p  < 0.0001, I 2  = 84%; MT, SMD = 0.69, 95% CI: 0.29 to 1.09, p  = 0.008, I 2  = 81%). WBVT showed no changes in KES (SMD = 0.65, 95% CI: − 0.02 to 1.31, p  = 0.06, I 2  = 80%) or GS (SMD = 0.12, 95% CI: − 0.15 to 0.39, p  = 0.38, I 2  = 0%). TUG times were significantly improved with all exercise training modes (SMD = -0.66, 95% CI: − 0.94 to − 0.38, p  < 0.00001, I 2  = 60%). There were no changes in CS times with any of the exercise training modes (SMD = 0.11, 95% CI: − 0.36 to 0.57, p  = 0.65, I 2  = 87%). Conclusions In older people with sarcopenia, KES and GS can be improved by RT and MT, but not by WBVT. All three training modes improved TUG times, but not improved CS times.

The NF-κB interacting LncRNA (NKILA) is a recently identified long noncoding RNA (lncRNA) located on chromosome 20q13.31. Studies have indicated that abnormal levels of NKILA expression in different types of cancer can function as either oncogenes or tumour suppressors and play a role in the development of diverse malignancies. Moreover, NKILA expression levels correlate closely with the clinical features and prognosis of cancer patients, underscoring its potential significance in clinical practice. Multiple studies have shown that NKILA acts as a competing endogenous RNA (ceRNA), participating in several crucial signalling pathways and interacting with proteins to regulate gene expression. Furthermore, NKILA affects essential aspects of cancer cell behaviour, such as proliferation, migration, invasion, apoptosis, the epithelial–mesenchymal transition (EMT), and resistance to treatment. NKILA downregulation is associated with increased tumour size, advanced pathological stage, increased lymph node metastasis, and poor patient prognosis. This article reviews the expression patterns, clinical relevance, molecular mechanisms, and biological functions of NKILA, investigates its potential clinical roles as a therapeutic target and diagnostic marker, and evaluates its efficacy in treating various tumours.

Effective pest management is important during the natural growth phases of cotton in the wild. As cotton fields are infested with \"tiny pests\" (smaller than 32×32 pixels) and \"very tiny pests\" (smaller than 16×16 pixels) during growth, making it difficult for common object detection models to accurately detect and fail to make sound agricultural decisions. In this study, we proposed a framework for detecting \"tiny pests\" and \"very tiny pests\" in wild cotton fields, named SRNet-YOLO. SRNet-YOLO includes a YOLOv8 feature extraction module, a feature map super-resolution reconstruction module (FM-SR), and a fusion mechanism based on BiFormer attention (BiFormerAF). Specially, the FM-SR module is designed for the feature map level to recover the important feature in detail, in other words, this module reconstructs the P5 layer feature map into the size of the P3 layer. And then we designed the BiFormerAF module to fuse this reconstruct layer with the P3 layer, which greatly improves the detection performance. The purpose of the BiFormerAF module is to solve the problem of possible loss of feature after reconstruction. Additionally, to validate the performance of our method for \"tiny pests\" and \"very tiny pests\" detection in cotton fields, we have developed a large dataset, named Cotton-Yellow-Sticky-2023, which collected pests by yellow sticky traps. Through comprehensive experimental verification, we demonstrate that our proposed framework achieves exceptional performance. Our method achieved 78.2% mAP on the \"tiny pests\" test result, it surpasses the performance of leading detection models such as YOLOv3, YOLOv5, YOLOv7 and YOLOv8 by 6.9%, 7.2%, 5.7% and 4.1%, respectively. Meanwhile, our results on \"very tiny pests\" reached 57% mAP, which are 32.2% higher than YOLOv8. To verify the generalizability of the model, our experiments on Yellow Sticky Traps (low-resolution) dataset still maintained the highest 92.8% mAP. The above experimental results indicate that our model not only provides help in solving the problem of tiny pests in cotton fields, but also has good generalizability and can be used for the detection of tiny pests in other crops.

This article empirically examines the \"responsibility-performance\" interaction in the process of environmental social responsibility decision-making by enterprises, using the listed companies in the heavy pollution industry as the research sample. We also use the evolutionary game theory to construct a system dynamics model for simulation analysis to explore the evolutionary qualities of the environmental responsibility behavior of heavily polluting enterprises and its relationship with financial performance under the influence of local government environmental regulation. It finds that for environmental social responsibility, a company's expected performance surplus is more likely to promote positive interaction between responsibility and performance than a gap; for regions with relatively negative environmental strategies, environmental social responsibility provides more efficient reputation transformation and motivates companies to invest in it; more competitive industries and enterprises with forward-looking environmental strategies tend to put performance feedback into the undertaking of environmental social responsibility. Therefore, the government and regulatory authorities should urge heavy polluting enterprises to implement environmental responsibility, so that they can fully realize that emission reduction and growth can be achieved at the same time and promote the financial performance of enterprises and environmental responsibility while forming a win-win situation at the ecological level and corporate social level.

Non-alcoholic fatty liver disease (NAFLD), also known as Metabolic dysfunction-associated fatty liver disease (MASLD), has become one of the most common chronic liver diseases worldwide, approximately 30% of adults and 70%~80% of patients with obesity and diabetes suffer from NAFLD. We attempted to find a potential hub gene in NAFLD hepatocyte cell model induced by palmitic acid and oil acid (PAOA), and investigated the function of the hub-gene. We searched and downloaded the GSE122660 dataset from GEO-DataSets, and differentially expressed genes (DEGs) were analyzed using R software. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to identify the significantly activated signaling pathways in steatohepatitis. A protein-protein interaction (PPI) network was constructed to identify hub genes among the DEGs. qRT-PCR, Western blotting, and Oil Red O staining were used to explore the function of hub genes in PAOA-induced hepatocytes in vitro. A total of 255 DEGs were identified in the GSE122660 dataset and were primarily associated with inflammation-and lipid metabolism-related pathways. The tribbles pseudokinase 3 (TRIB3) was highlighted as a hub gene. We found that TRIB3 was upregulated in CDHFDinduced NAFLD mouse liver tissue and hepatocyte cell models. Furthermore, TRIB3 aggravated PAOA-induced lipid accumulation and inflammation in hepatocytes in vitro. The present study identified TRIB3 as a hub gene for steatohepatitis and aggravated lipid accumulation and inflammation in vitro. Therefore, targeting TRIB3 could be a potential pharmacological strategy for NAFLD treatment.

The objective of this study was to systematically review the literature on the effects of cognitive behavioral therapy (CBT) on insomnia and pain in patients with traumatic brain injury (TBI). PubMed, Embase, the Cochrane Library, Cumulative Index to Nursing and Allied Health, and Web of Science databases were searched. Outcomes, including pain, sleep quality, and adverse events, were investigated. Differences were expressed using mean differences (MDs) with 95% confidence intervals (CIs). The statistical analysis was performed using STATA 16.0. Twelve trials with 476 TBI patients were included. The included studies did not indicate a positive effect of CBT on pain. Significant improvements were shown for self-reported sleep quality, reported with the Pittsburgh Self-Reported Sleep Quality Index (MD, -2.30; 95% CI, -3.45 to -1.15; P<0.001) and Insomnia Severity Index (MD, -5.12; 95% CI, -9.69 to -0.55; P=0.028). No major adverse events related to CBT were reported. The underpowered evidence suggested that CBT is effective in the management of sleep quality and pain in TBI adults. Future studies with larger samples are recommended to determine significance. This trial is registered with PROSPERO registration number CRD42019147266.

Hypoxia is an unfavorable environmental condition that produces diverse negative effects in fish. High-density cultures of Epinephelus coioides are more likely to experience hypoxic conditions than those in natural environments. To assess the effects of hypoxia on E. coioides , we examined the related enzyme activities and gene expression after 48 h of hypoxia and 24 h of dissolved oxygen (DO) recovery. Under hypoxic stress (DO: 1.2 ± 0.1 mg/L), the energy supply mode of fish changed from aerobic metabolism to anaerobic metabolism, and the serum glucose content and lactate dehydrogenase activity were significantly upregulated. Total protein, hepatic glycogen, and two key regulatory enzymes (i.e., hexokinase and pyruvate kinase) were differentially expressed in the liver, and mRNA expression of three genes (i.e., LDHA , GLUT1 , and MCT2 ) also showed a high expression trend. In serum, three immune-related enzymes (i.e., alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase) were found to be involved in regulation by hypoxia and showed different levels of changing patterns. Expression of inflammatory genes (i.e., IL-8 , IFNγ , MyD88 , and NF-kB ) were significantly regulated in liver. With prolongation of hypoxic stress, high expression of apoptotic genes (i.e., p53 , Bax , Bcl-2 , and Caspase-9 ) was closely related to the degree of apoptosis in the liver. Our investigation of the changes in energy metabolism, immune response, and apoptosis of E. coioides under hypoxia and reoxygenation (DO, 6.0 ± 0.1 mg/L) provides a theoretical bases for healthy aquaculture and selection of varieties with tolerance to hypoxia.

Several exogenous probiotics are applicable in fish culture; however, challenges in isolation and verification have hindered the full utilization of numerous host probiotics. Therefore, this study aimed to apply the host probiotic Exiguobacterium acetylicum G1-33 to hybrid grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂) cultures and explore its mechanism of action. In total, 360 hybrid grouper were divided into four groups, which were fed the following for 60 days: three received commercial feed with varying concentrations of E. acetylicum G1-33 (106, 108, and 1010 CFU/g), while a control group received commercial feed. The results showed that supplementation with 106 and 108 CFU/g of E. acetylicum G1-33 enhanced gut morphology, upregulated growth-related genes (ghr1, igf-2, s6k1, tor), and promoted growth, with supplementation with 108 CFU/g resulting in the most notable enhancement. However, supplementation with 1010 CFU/g inhibited growth, possibly because of changes in intestinal morphology. Additionally, supplementation with E. acetylicum G1-33 upregulated the expression of immune-related genes (c3, myd88, Cu/Zn-sod, tlr3, and tnf2) in the liver and head kidney but led to an increase in malondialdehyde content, as well as a decrease in alkaline phosphatase and acid phosphatase activities, in the liver and serum, indicating increased oxidative stress. Moreover, supplementation with 106 and 108 CFU/g E. acetylicum G1-33 enhanced the widespread expression of immune-related genes in the head kidney and liver, respectively, and improved resistance to Vibrio harveyi, whereas supplementation with 1010 CFU/g weakened this resistance. In conclusion, E. acetylicum G1-33, particularly at 108 CFU/g, emerged as an effective probiotic, optimizing growth performance and immunity in hybrid grouper. This research is pioneering in its application of E. acetylicum in mariculture, potentially broadening the range of probiotic strategies in aquaculture.

This study aims to examine the effects of the mixture of Bacillus cereus G1–11 and Exiguobacterium acetylicum G1–33, isolated from the gut of hybrid groupers (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂), on the host. The hybrid groupers were divided into a control (C, without any probiotics), B. cereus (BC, 1010 cfu/g), E. acetylicum (EA, 108 cfu/g), compound (mix, a 1:1 mixture of B. cereus and E. acetylicum), and positive reference group (P, Lactobacillus acidophilus, 5 × 108 cfu/L). Each group had four replicates, with 30 fish per replicate (53.30 ± 0.50 g), and were fed for 60 days. The results showed that adding probiotics to the feed significantly improved the weight gain, weight growth rate, specific growth rate, and digestive enzyme activities of hybrid groupers compared to the C group. The compound group was the most significant. In addition, composite probiotics added to feed significantly upregulated the expression levels of several growth-related genes in the liver and muscles. The activities of alkaline phosphatase, catalase, glutathione peroxidase, glutathione transferase, lysozyme, and total antioxidant capacity in the serum and liver were significantly influenced through mixed probiotic feeding. Moreover, the expression levels of several immune-related genes in the liver, spleen, and head kidney were significantly enhanced by adding single and mixed probiotics to feed, with the synergy of mixed probiotics being the best. An analysis of the gut microbiota showed that adding composite bacteria enhanced the richness and diversity of the gut microbiota, significantly increasing the relative abundance of potential probiotics (Cetobacterium and Microbacterium) while decreasing the presence of potential pathogens (Mycoplasma). Overall, our findings highlighted the efficacy of mixed probiotics (B. cereus and E. acetylicum) in enhancing growth performance, nutritional value of hybrid grouper feed, antioxidant capacity, immune response, and intestinal health, in finding the best combination of functional feed additives.

The Zig-zag eel ( Mastacembelus armatus ) is an economically important species in southern China. Its natural resources have declined year by year due to overfishing. Understanding its genetic diversity and population structure is very important for resource conservations. Here, we first successfully developed 28 polymorphic microsatellite markers for zig-zag eels and ten of them were used to examine the genetic diversity and differentiation of 7 populations collected from the major river systems of south China. In total, 224 alleles were found with the 10 microsatellite loci in 7 populations, ranging from 4.6 (Nandujiang: NDJ) to 11.1 (Xijiang, XJ), with an average of 8.871 alleles. The average observed and expected heterozygosity ranged from 0.550 (NDJ) to 0.964 (Yuangjiang, YJ) and from 0.537 (NDJ) to 0.775 (Tanjiang, TJ), respectively. The average polymorphism-information content ranged from 0.472 (NDJ) to 0.757 (TJ). Hardy–Weinberg equilibrium test results revealed the loci showed differing deviation in different populations. In total, low level of genetic diversity was only found in HJ (Hanjiang) and NDJ populations. Besides, evidence of recent bottleneck was found in the HJ populations. Analysis of molecular variation showed that the percent variation within individuals (75.00%) was higher than that among populations (25%). In addition, population structure and the pairwise F ST revealed that there was low differentiation among XJ, TJ and YJ populations. These data provide important genetic resources for understanding the population differentiation and facilitating genetic conservation and utilization of this species. Article highlights A total of 28 polymorphic microsatellite markers were successfully developed for zig-zag eels. Low level of genetic diversity was only found in HJ and NDJ populations. Low differentiation was found among XJ, TJ and YJ populations.