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Chestnuts are multipurpose trees significant for the economy and wildlife. These trees are currently found around the globe, demonstrating their genetic adaptation to different environmental conditions. Several biotic and abiotic stresses have challenged these species, contributing to the decline of European chestnut production and the functional extinction of the American chestnut. Several efforts started over the last century to understand the cellular, molecular, and genetic interactions behind all chestnut biotic and abiotic interactions. Most efforts have been toward breeding for the primary diseases, chestnut blight and ink disease caused by the pathogens, Cryphonectria parasitica and Phytophthora cinnamomi , respectively. In Europe and North America, researchers have been using the Asian chestnut species, which co-evolved with the pathogens, to introgress resistance genes into the susceptible species. Breeding woody trees has several limitations which can be mostly related to the long life cycles of these species and the big genome landscapes. Consequently, it takes decades to improve traits of interest, such as resistance to pathogens. Currently, the availability of genome sequences and next-generation sequencing techniques may provide new tools to help overcome most of the problems tree breeding is still facing. This review summarizes European and American chestnut’s main biotic stresses and discusses breeding and biotechnological efforts developed over the last decades, having ink disease and chestnut blight as the main focus. Climate change is a rising concern, and in this context, the adaptation of chestnuts to adverse environmental conditions is of extreme importance for chestnut production. Therefore, we also discuss the abiotic challenges on European chestnuts, where the response to abiotic stress at the genetic and molecular level has been explored.

Phytophthora cinnamomi Rands devastates forest species worldwide, causing significant ecological and economic impacts. The European chestnut ( Castanea sativa ) is susceptible to this hemibiotrophic oomycete, whereas the Asian chestnuts ( Castanea crenata and Castanea mollissima ) are resistant and have been successfully used as resistance donors in breeding programs. The molecular mechanisms underlying the different disease outcomes among chestnut species are a key foundation for developing science-based control strategies. However, these are still poorly understood. Dual RNA sequencing was performed in C. sativa and C. crenata roots inoculated with P. cinnamomi. The studied time points represent the pathogen’s hemibiotrophic lifestyle previously described at the cellular level. Phytophthora cinnamomi expressed several genes related to pathogenicity in both chestnut species, such as cell wall–degrading enzymes, host nutrient uptake transporters, and effectors. However, the expression of effectors related to the modulation of host programmed cell death ( elicitins and NLPs ) and sporulation-related genes was higher in the susceptible chestnut. After pathogen inoculation, 1,556 and 488 genes were differentially expressed by C. crenata and C. sativa , respectively. The most significant transcriptional changes occur at 2 h after inoculation (hai) in C. sativa and 48 hai in C. crenata . Nevertheless, C. crenata induced more defense-related genes, indicating that the resistant response to P. cinnamomi is controlled by multiple loci, including several pattern recognition receptors, genes involved in the phenylpropanoid, salicylic acid and ethylene/jasmonic acid pathways, and antifungal genes. Importantly, these results validate previously observed cellular responses for C. crenata . Collectively, this study provides a comprehensive time-resolved description of the chestnut– P. cinnamomi dynamic, revealing new insights into susceptible and resistant host responses and important pathogen strategies involved in disease development.

Baroreceptor and chemoreceptor reflexes modulate inflammatory responses. However, whether these reflexes attenuate periodontal diseases has been poorly examined. Thus, the present study determined the effects of electrical activation of the carotid sinus nerve (CSN) in rats with periodontitis. We hypothesized that activation of the baro and chemoreflexes attenuates alveolar bone loss and the associated inflammatory processes. Electrodes were implanted around the CSN, and bilateral ligation of the first mandibular molar was performed to, respectively, stimulate the CNS and induce periodontitis. The CSN was stimulated daily for 10 min, during nine days, in unanesthetized animals. On the eighth day, a catheter was inserted into the left femoral artery and, in the next day, the arterial pressure was recorded. Effectiveness of the CNS electrical stimulation was confirmed by hypotensive responses, which was followed by the collection of a blood sample, gingival tissue, and jaw. Long-term (9 days) electrical stimulation of the CSN attenuated bone loss and the histological damage around the first molar. In addition, the CSN stimulation also reduced the gingival and plasma pro-inflammatory cytokines induced by periodontitis. Thus, CSN stimulation has a protective effect on the development of periodontal disease mitigating alveolar bone loss and inflammatory processes.

BackgroundAir quality deteriorates significantly during wildfire events, which poses a risk for the health of affected human populations. The Mediterranean Basin was strongly impacted by wildfires during the 2021 fire season, particularly in Greece.AimsThis work aims at estimating the impact of the Greek wildfires of August 2021 on the air quality in Athens.MethodsThe numerical modelling system WRF-APIFLAME-CHIMERE, which comprises a meteorological model, a smoke emissions model and a chemical transport model, was employed in estimating the hourly three-dimensional distribution of particulate matter (PM), CO and O3 concentrations during the wildfires. The performance of the modelling system was evaluated by comparing modelled results with air quality observations and atmospheric optical depth measurements.Key resultsGood agreement between measured data and model results was found, with results obtained with a higher-resolution computational grid performing the best.ConclusionsThe calculated values indicate concerning hourly and daily levels of air pollution, above the limit values for human health protection, during the analysed days within and around Athens.ImplicationsThe results highlight the importance of implementing a strategy for human health protection during wildfire events affecting populated areas. This modelling approach could be a basis for a smoke forecasting system.

Canine cutaneous leishmaniasis (CCL) is a zoonosis of public health interest, and in the Americas, Leishmania (Viannia) braziliensis has been identified as the main etiological agent. The present study sought to investigate Leishmania spp. infection in domestic dogs from a rural area of the Xapuri municipality, Acre state, Brazilian Amazonia. For this purpose, visits were carried out to domiciles where the human cases of American cutaneous leishmaniasis (ACL) occurred, followed by the clinical evaluation of the animals in search of clinical signs suggestive of CCL. Blood samples were collected from 40 dogs, 13 of which had lesions suggestive of CCL, and biopsies of these lesions were performed. The methods used were Neal, Novy, and Nicolle's (NNN) medium cultures and direct parasitological examination. Further, to detect and characterize Leishmania DNA some molecular techniques were performed such as conventional polymerase chain reaction (PCR) and sequencing targeting SSU rDNA and ITS1, restriction fragment length polymorphism (RFLP) and high resolution melting (HRM) analysis targeting hsp70. The investigation revealed that the results obtained from the parasitological methods were negative. In PCR by ITS1 and network topology sequences, six strains from dogs, isolated from the Peruvian Andes, appeared identical to Leishmania (Viannia) braziliensis type 2 (99-100%). By other molecular methods these samples turned out to be positive to Leishmania (Viannia) sp.. The diagnosis of Leishmania in domestic dogs from Acre state showed a high proportion of infected animals, and the occurrence of L. braziliensis type 2 in Brazil for the first time. This new report suggests that L. braziliensis type 2 is both trans- and cis-Andean. However, more studies are needed regarding the clinical and diagnostic aspects of this species of Leishmania.

Background: Oral mucositis (OM) is one of the most important acute toxicities from radiotherapy (RT) in head and neck cancer patients and can impair oncologic treatment. Dysphagia, dysgeusia, pain, and oral candidiasis are other common toxicities. Brazilian Organic Propolis (BOP) is a recently described propolis variant and BOP types 4 and 6 have shown important antioxidant, anti-inflammatory, and antifungal properties. Purpose: To investigate the use of BOP as a preventive and/or complementary therapeutic option for radiotherapy-induced oral mucositis, dysphagia, dysgeusia, pain, and oral candidiasis. Additionally, proinflammatory cytokines were assessed to investigate their anti-inflammatory role. Methods: Sixty patients were included in this randomized, double-blind, controlled clinical trial. Patients were randomized to receive either aqueous suspension of a BOP or placebo throughout RT. Also, all patients underwent low-level laser therapy as routine oral care. OM, dysphagia, and dysgeusia were assessed weekly according to WHO and NCI scales. Pain-related to OM was assessed according to a Visual Analog Scale and the presence or absence of oral candidiasis was checked by intraoral examination. Protein levels of TNF-α and IL-1β from oral mucosa were assessed by ELISA. Results: Patients in the propolis group had a lower mean score of OM, dysphagia, dysgeusia, and most patients reported moderate pain. Fewer patients developed oral candidiasis in the propolis group, and the number of episodes was lower among patients that used BOP ( p < 0.05). In addition, the BOP group presented significantly lower levels of IL-1β since the beginning of treatment when compared with placebo patients ( p < 0.05) and a lower level of TNF-α at the end of treatment ( p < 0.001). Conclusion: Topic use of BOP reduced TNF-α and IL-1β levels, oral candidiasis episodes, and seems to be a useful complementary option for the prevention and treatment of the main acute oral toxicities of RT. Clinical Trial Registration: http://www.ensaiosclinicos.gov.br/rg/RBR-9f8c78/ , identifier RBR-9f8c78

Interest in sustainable construction has been increasing due to recent events. The limitations of natural resources and the scale of global impacts, specifically as a result of the effects of global climate change, have consequences for the construction sector. These changes are giving rise to a need to reassess the way we face the built environment and rethink new solutions for construction systems or methods that contribute to mitigating negative consequences, among which we highlight the prefabrication method. This new scenario, characterised by the need to meet the decarbonisation goals set for 2050, as well as the effects of the spread of the pandemic crisis, emphasizes the importance of understanding the impacts that may occur in the construction industry, which are essentially understood as increases in sustainability, productivity, quality and, consequently, as reductions in deadlines, costs, and dependence on labour. Therefore, this entry seeks to study on the existing literature on prefabrication, seeking to gather relevant information on the new advances, challenges, and opportunities of this construction method whose approach has been mostly focused on partial or specific aspects for case studies, both highlighting the potential and identifying the gaps and opportunities of prefabrication in this new context. The prefabrication method brings benefits compared to the conventional method, and may be an alternative, as it has more positive global impacts on the environment, the economy, and society, and consequently on the sustainable development of construction, despite some limitations that have been reported and that should be looked into in the future.

Microencapsulation procedures have recently focused attention on designing novel microspheres via green synthesis strategies. The use of chitosan (CS) as an encapsulating material has increased interest due to its unique bioactive properties and the various crosslinking possibilities offered by their functional groups. The consolidation of the microspheres by physical crosslinking using sodium tripolyphosphate (TPP) combined with chemical crosslinking using vanillin (VA) open new opportunities in the framework of green dual crosslinking strategies. The developed strategy, a straightforward technique based on an aqueous medium avoiding complex separation/washing steps, offers advantages over the processes based on VA, mostly using water-in-oil emulsion approaches. Thus, in this work, the combination of TPP crosslinking (3, 5, and 10 wt.%) via spray-coagulation technique with two VA crosslinking methods (in situ and post-treatment using 1 wt.% VA) were employed in the preparation of microspheres. The microspheres were characterized concerning morphology, particle size, physicochemical properties, thermal stability, and swelling behavior. Results revealed that the combination of 5 wt.% TPP with in situ VA crosslinking led to microspheres with promising properties, being an attractive alternative for natural bioactives encapsulation due to the green connotations associated with the process.

People spend most of their time in indoor environments without knowing about the air quality in these spaces. In this study, indoor low-cost sensors were used (for 5 months) to assess the comfort and air quality patterns in two indoor households. To strengthen the robustness of the considered approach and build confidence in the obtained comfort and indoor air quality (IAQ) levels, the sensor measurements were also compared against information from reference monitoring equipment; in which, high correlation coefficients were obtained (> 0.85) and also low errors (on average 22%). The IAQ results were strongly influenced by the residents’ activity and behaviour, the outdoor weather conditions, and indoor/outdoor air pollution sources. Overall, the recommended values of temperature and relative humidity for the occupant’s comfort in indoor environments were not fulfilled. The highest particulate matter (PM) levels were recorded at the weekend (on average +14% higher), while maximum CO 2 and CO levels were obtained on the weekdays (on average +9% higher). PM daily profiles followed the outdoor concentrations with the maximum levels at the end of the night and the lowest values in the early morning/mid-afternoon. The highest and lowest CO 2 concentrations were registered in the early morning (< 1536 ppm) and mid-afternoon (< 627 ppm), respectively, while the CO daily profiles showed a high impact of outdoor emissions, with the minimum concentrations up to 0.81 mg m −3 (at 10 a.m. or 6 p.m.), and a maximum concentration of 1.87 mg m −3 (at 10 p.m.). Real-time comfort conditions and IAQ levels are a powerful approach to providing fast decisions to minimise human exposure and prevent negative health impacts.

The pinewood nematode (PWN), Bursaphelenchus xylophilus , responsible for the pine wilt disease (PWD), is a major threat to pine forests worldwide. Since forest mortality due to PWN might be exacerbated by climate, the concerns regarding PWD in the Mediterranean region are further emphasized by the projected scenarios of more drought events and higher temperatures. In this context, it is essential to better understand the pine species vulnerability to PWN under these conditions. To achieve that, physiological responses and wilting symptoms were monitored in artificially inoculated Pinus pinaster ( P. pinaster ), Pinus pinea ( P. pinea ), and Pinus radiata ( P. radiata ) saplings under controlled temperature (25/30°C) and water availability (watered/water stressed). The results obtained showed that the impact of PWN is species-dependent, being infected P. pinaster and P. radiata more prone to physiological and morphological damage than P. pinea . For the more susceptible species ( P. pinaster and P. radiata ), the presence of the nematode was the main driver of photosynthetic responses, regardless of their temperature or water regime conditions. Nevertheless, water potential was revealed to be highly affected by the synergy of PWN and the studied abiotic conditions, with higher temperatures ( P. pinaster ) or water limitation ( P. radiata ) increasing the impact of nematodes on trees’ water status. Furthermore, water limitation had an influence on nematodes density and its allocation on trees’ structures, with P. pinaster revealing the highest nematode abundance and inner dispersion. In inoculated P. pinea individuals, nematodes’ population decreased significantly, emphasizing this species resistance to PWN. Our findings revealed a synergistic impact of PWN infection and stressful environmental conditions, particularly on the water status of P. pinaster and P. radiata , triggering disease symptoms and mortality of these species. Our results suggest that predicted drought conditions might facilitate proliferation and exacerbate the impact of PWN on these two species, through xylem cavitation, leading to strong changes in pine forests of the Mediterranean regions.