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Background: There are few studies on the evaluation of meat from hybrid sorubins. Objective: Thus, the aim of this work was to evaluate the physical, chemical, microbiological and sensorial characteristics of fillets of Amazon and real hybrid sorubins. Method: Proximate composition, instrumental colour, water holding capacity, cooking losses and shear force were measured. Microbiological analyses of the fillets were carried out for Salmonella sp. and Staphylococcus aureus to ensure food safety of the judges during sensory analysis. Results: Hybrid sorubim fillets presented an excellent quality, being classified in category A (lipids below 5% and proteins between 15% and 20%). There was no significant difference (p>0.05) between the evaluated fillets for several parameter: the average water holding capacities were 33.72 and 34.67%, the cooking losses were 14.93 and 13.41%, the shear forces were 2.21 and 1.74 kgf, and the luminosities were 49.61 and 45.04 for the fillets of Amazonian hybrid sorubim and real hybrid sorubim, respectively. Discussion: There was an evident relation between water holding capacities and shear forces. Amazonian hybrid sorubim fillets were more accepted due to their lower intensity of red. However, there was no sensorial difference between the hybrids, and both had an acceptance rate above 80%. Conclusion: It was concluded that the hybridization does not alter the sensory characteristics of the fish fillets.

Strains belonging to the yeast species Kluyveromyces marxianus have been isolated from a great variety of habitats, which results in a high metabolic diversity and a substantial degree of intraspecific polymorphism. As a consequence, several different biotechnological applications have been investigated with this yeast: production of enzymes (β-galactosidase, β-glucosidase, inulinase, and polygalacturonases, among others), of single-cell protein, of aroma compounds, and of ethanol (including high-temperature and simultaneous saccharification-fermentation processes); reduction of lactose content in food products; production of bioingredients from cheese-whey; bioremediation; as an anticholesterolemic agent; and as a host for heterologous protein production. Compared to its congener and model organism, Kluyveromyces lactis, the accumulated knowledge on K. marxianus is much smaller and spread over a number of different strains. Although there is no publicly available genome sequence for this species, 20% of the CBS 712 strain genome was randomly sequenced (Llorente et al. in FEBS Lett 487:71-75, 2000). In spite of these facts, K. marxianus can envisage a great biotechnological future because of some of its qualities, such as a broad substrate spectrum, thermotolerance, high growth rates, and less tendency to ferment when exposed to sugar excess, when compared to K. lactis. To increase our knowledge on the biology of this species and to enable the potential applications to be converted into industrial practice, a more systematic approach, including the careful choice of (a) reference strain(s) by the scientific community, would certainly be of great value.

 Microalga is one of the main sources of biomasses for the production of biofuels in the 21st century. Many labs and industries around the world are seeking for more productive strains and better cultivation models. The aim of this work was to evaluate the growth and kinetic parameters of six microalgae, and the moisture and lipid contents and fatty acids profiles of the biomasses harvested at the end of each cultivation. The microalgae were isolated in the Mid-Western Region of Brazil cultivated in open pond systems. It was observed that among the six microalgae used in this work, Pseudokirchneriella subcapitata presented the higher maximum specific growth rate (μmax) (0.12 day-1). The microalgae Coelastrum sp. and P. subcapitata were the ones with the highest lipid contents, with approximately 20% of dry mass. The main fatty acid accumulated in these conditions was palmitic acid, with percentages of 59 to 69% for all microalgae evaluated. Other factors that had influenced results from cultivations in open ponds were discussed here. 

The yeast Kluyveromyces marxianus has been pointed out as a promising microorganism for a variety of industrial bioprocesses. Although genetic tools have been developed for this yeast and different potential applications have been investigated, quantitative physiological studies have rarely been reported. Here, we report and discuss the growth, substrate consumption, metabolite formation, and respiratory parameters of K . marxianus CBS 6556 during aerobic batch bioreactor cultivations, using a defined medium with different sugars as sole carbon and energy source, at 30 and 37 °C. Cultivations were carried out both on single sugars and on binary sugar mixtures. Carbon balances closed within 95 to 101 % in all experiments. Biomass and CO 2 were the main products of cell metabolism, whereas by-products were always present in very low proportion (<3 % of the carbon consumed), as long as full aerobiosis was guaranteed. On all sugars tested as sole carbon and energy source (glucose, fructose, sucrose, lactose, and galactose), the maximum specific growth rate remained between 0.39 and 0.49 h −1 , except for galactose at 37 °C, which only supported growth at 0.31 h −1 . Different growth behaviors were observed on the binary sugar mixtures investigated (glucose and lactose, glucose and galactose, lactose and galactose, glucose and fructose, galactose and fructose, fructose and lactose), and the observations were in agreement with previously published data on the sugar transport systems in K. marxianus . We conclude that K. marxianus CBS 6556 does not present any special nutritional requirements; grows well in the range of 30 to 37 °C on different sugars; is capable of growing on sugar mixtures in a shorter period of time than Saccharomyces cerevisiae , which is interesting from an industrial point of view; and deviates tiny amounts of carbon towards metabolite formation, as long as full aerobiosis is maintained.

Sausages, one of the oldest forms of meat processing, rely heavily on the quality of casings to achieve desired texture, appearance, and stuffing efficiency. This study aimed to improve the hydration process of natural pork casings for Calabrian sausage production by employing phosphoric acid as a substitute for trisodium phosphate dodecahydrate. Key quality control tools, including the Ishikawa diagram, 5W1H tool, and PDCA cycle, were utilized to identify and address production inefficiencies. The implemented modifications led to significant advancements: stuffing yield increased from 0.87 to 1.0 kg/m over seven months, surpassing the recommended standard of 0.91 kg/m, and casing consumption decreased by 10.39%. These improvements not only enhanced operational efficiency but also reduced casing costs by 10.37%. The findings confirm the viability of phosphoric acid as an effective, cost-efficient alternative in the hydration process, paving the way for improved sausage production standards.

In this study, the potential of ultraviolet B (UV-B) radiation to alleviate the effects of pollutants in cigarette butt wastewater (CBW) was investigated using different Chlorella sorokiniana strains (F4, R1 and LG1). Microalgae were treated with UV-B (1.7 W m−2) for 3 days prior to their exposure to CBW and then incubated for 4 days in the absence or presence of UV-B. UV-B-untreated microalgae were used as the control. Comparative physiological responses, including photosynthetic pigments and non-enzymatic antioxidants, as well as nicotine and nicotyrine removal, were evaluated in 7-day cultures. UV-B treatments did not negatively impact algal chlorophyll or carotenoid production. UV-B acclimation was strain-dependent, correlating with native environment adaptations and genetic constitutions. UV-B as a pretreatment had long-term positive effects on non-enzymatic antioxidant capacity. However, LG1 needed more time to readjust the pro-oxidant/antioxidant balance, as it was the most UV-B-sensitive. Phenolic compounds played an important role in the antioxidant system response to UV-B, while flavonoids did not contribute to the total antioxidant capacity. Although cross-resistance between UV-B and CBW was observed in F4 and R1, only R1 showed nicotine/nicotyrine catabolism induction due to UV-B. Overall, the results suggest that UV-B activates defense pathways associated with resistance or tolerance to nicotine and nicotyrine.

Jackfruit seeds are usually discarded as waste. Therefore, this work aimed at obtaining the Jackfruit seeds flour of two varieties (soft and firm) from Brazilian Midwest and to evaluate its physicochemical characteristics, mineral and fatty acids composition (FA). Seeds were dried at 45°C for 72h and ground (40-mesh). Both flours revealed high protein content, varying from 13.43% to 16.28%. It also revealed excellent insoluble fibers (4.16% for soft Jackfruit seeds flour [SJF] and 4.68% for firm Jackfruit seeds flours [FJF]) content. Plus, it was good sources of minerals, especially magnesium (approximately 200 mg 100g-1). Oleic acid was predominant in FJF (47.03%) and linoleic acid (30.81%) in SJF. The flour from both seeds presented minor contents of acid α-linolenic acid (approximately 5%). Consequently, both seeds flours are strongly indicated for formulating new food products, improving its nutritional value and promoting beneficial effects on human health.

Chitosan is a biopolymer of wide application due to its characteristics and non-toxicity, presenting antimicrobial, antitumoral and cicatrizing activities. It is currently used as emulsifier, metal's chelating, edible biofilm and fat reducer. The variation in the deacetylation degree of this polymer gives differentiated functional properties. It is mainly obtained from crustaceans, but fish scales are also a potential source of this product, despite neglected so far. The aim of this study was to develop a method for chitin extraction and deacetylation for chitosan obtaining from Nile tilapia (Oreochromis niloticus) scales. Characterization showed that chitosan was completely purified. The chitin infrared spectrum presented a characteristic larger band in the region of 3,500 [cm.sup.-1], due to axial stretching vibrations of the OH group been completely purified, which disappeared in the chitin spectrum. However, a new band aroused at 1,640 [cm.sup.-1] due to the N[H.sub.2] deformation, which predominated over the band at 1,655 [cm.sup.-1], associated to the carbonyl (C=O) that tends to decrease, as the degree of deacetylation of chitosan increases. All bands observed were similar to those described in the literature. Although the yields were lower than the averages usually reported for crustaceans, they can be improved to obtain higher yields and deacetylation.

Microalgal-based remediation is an ecofriendly and cost-effective system for wastewater treatment. This study evaluated the capacity of microalgae in the remediation of wastewater from cleaning process of smoked cigarette butts (CB). At laboratory scale, six strains (one from the family Scenedesmaceae, two Chlamydomonas debaryana and three Chlorella sorokiniana) were exposed to different CB wastewater dilutions to identify toxicity levels reflected in the alteration of microalgal physiological status and to determine the optimal conditions for an effective removal of contaminants. CB wastewater could impact on microalgal chlorophyll and carotenoid production in a concentration-dependent manner. Moreover, the resistance and remediation capacity did not only depend on the microalgal strain, but also on the chemical characteristics of the organic pollutants. In detail, nicotine was the most resistant pollutant to removal by the microalgae tested and its low removal correlated with the inhibition of photosynthetic pigments affecting microalgal growth. Concerning the optimal conditions for an effective bioremediation, this study demonstrated that the Chlamydomonas strain named F2 showed the best removal capacity to organic pollutants at 5% CB wastewater (corresponding to 25 butts L−1 or 5 g CB L−1) maintaining its growth and photosynthetic pigments at control levels.

The Brazilian savannah is the second largest biome of the country, and it displays great biodiversity. The fruits of the native trees have peculiar characteristics and are recognized for their nutritional and therapeutic aspects. However, little is known about their technological and biotechnological potential applications. The existing information concerning these aspects has never been compiled so far. It is known that many of these fruits contain many bioactive compounds of industrial interest, such as carotenoids and phenolic constituents. Another aspect of the fruit is the high fatty acid content of some species. Pequi, bocaiuva, jatoba, baru, amburama, and buriti, for instance, are among those fruits described as being rich in fatty acids, mostly unsaturated ones. Here, we reviewed 18 species from the Brazilian savannah identified to be of interest because of high potencial for sustained medium- and short-term explorations, under the technological and biotechnological aspects, seeking the development of new products from these scarcely studied raw materials.