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2-Phenylethanol (2-PE) and 2-phenethyl acetate (2-PEA) are important aroma compounds widely used in food and cosmetic industries due to their rose-like odor. Nowadays, due to the growing demand for natural products, the development of bioprocesses for obtaining value-added compounds has become of great significance. 2-PE and 2-PEA can be produced through the biotransformation of L-phenylalanine using the generally recognized as safe strain Kluyveromyces marxianus. L-phenylalanine bioconversion systems have been typically focused on submerged fermentation processes (SmF), but there is no information about other alternative productive approaches. Here, the solid-state fermentation (SSF) of sugarcane bagasse supplemented with L-phenylalanine was investigated as a sustainable alternative for producing 2-PE and 2-PEA in a residue-based system using Kluyveromyces marxianus as inoculum. An initial screening of the operational variables indicated that air supply, temperature, and initial moisture content significantly affect the product yield. Besides, it was found that the feeding strategy also affects the production and the efficiency of the process. While a basic batch system produced 16 mgproducts per gram of residue (dry basis), by using split feeding strategies (fed-batch) of only sugarcane bagasse, a maximum of 18.4 mgProducts g−1residue were achieved. Increase in product yield was also accompanied by an increase in the consumption efficiency of nutrients and precursor. The suggested system results as effective as other more complex SmF systems to obtain 2-PE and 2-PEA, showing the feasibility of SSF as an alternative for producing these compounds through the valorization of an agro-industrial residue.

Forest understories play a vital role in ecosystem functioning and the provision of ecosystem services. However, the extent to which environmental conditions drive dominant ecological strategies in forest understories at the continental scale remains understudied. Here, we used ~29 500 forest vegetation plots sampled across Europe and classified into 25 forest types to explore the relative role of macroclimate, soil pH and tree canopy cover in driving abundance-weighted patterns in the leaf economic spectrum (LES) and plant size spectrum (PSS) of forest understories (shrub and herb layers). We calculated LES using specific leaf area (SLA) and leaf dry matter content (LDMC) and PSS using plant height and seed mass of vascular plant species found in the understories. We found that forest understories had more conservative leaf economics in areas with more extreme mean annual temperatures (mainly Fennoscandia and the Mediterranean Basin), more extreme soil pH and under more open canopies. Warm and summer-dry regions around the Mediterranean Basin and areas of Atlantic Europe also had taller understories with heavier seeds than continental temperate or boreal areas. Understories of broadleaved deciduous forests, such as Fagus forests on non-acid soils, or ravine forests, more commonly hosted species with acquisitive leaf economics. In contrast, some coniferous forests, such as Pinus, Larix and Picea mire forests, or Pinus sylvestris light taiga and sclerophyllous forests, more commonly hosted species with conservative leaf economics. Our findings highlight the importance of macroclimate and soil factors in driving trait variation of understory communities at the continental scale and the mediator effect of canopy cover on these relationships. We also provide the first maps and analyses of LES and PSS of forest understories across Europe and give evidence that the understories of European forest types are differently positioned along major axes of trait variation.

Sophorolipids (SLs) are microbial biosurfactants with an important role in industry and a continuously growing market. This research addresses the use of sustainable resources as feedstock for bioproducts. Winterization oil cake (WOC) and molasses are suitable substrates for SLs via solid-state fermentation (SSF). The model proposed herein was established for annually processing 750 t of WOC and comparing three support materials: wheat straw (WS), rice husk (RH), and coconut fiber (CF). Production capacity ranged 325–414 t of SLs per year. Unit Production Cost was 5.1, 5.7, and 6.9 USD/kg SL for WS, RH, and CF production models, respectively, and was slightly lower with other substrates. Financial parameters were CAPEX 6.7 MM USD and OPEX 1.9 MM USD/y, with a NPV, IRR and payback time of 6.4 MM USD, 31% and 3.2 y, respectively. SLs recovery from the solid matrix was the major contributor to operating costs, while fermentation equipment shaped capital costs. Results show that the physical properties (bulk density, WHC) of substrates and supports define process costs beyond substrate purchase costs and process yields in SSF systems. To our knowledge, this is the first attempt to model SLs production via SSF at full scale for the economic valuation of the SSF process.

Background: The pa´ramo is a high-elevation biogeographical province in the northern Andes, known for its great biodiversity and ecosystem services. Because there have been very few biogeographic studies encompassing the entire province to date, this study aimed at conducting a phytogeographical regionalisation of the pa´ramo. Specifically, (1) clustering analyses were conducted to identify the main phytogeographical units in the three altitudinal belts: sub-pa´ramo, mid-pa´ramo and super-pa´ramo, and examine their diagnostic flora, (2) an ordination complemented the geo-climatic characterization of the obtained units and (3) a hierarchical classification transformation was obtained to evaluate the relationships between units. Methods: The study area included the entire Andean pa´ramo range in northern Peru, Ecuador, Colombia and Venezuela. The analyses were based on 1,647 phytosociological plots from the VegPa´ramo database. The K-means nonhierarchical clustering technique was used to obtain clusters identifiable as phytogeographical units, and the Ochiai fidelity index was calculated to identify their diagnostic species. A principal component analysis was conducted to obtain the geo-climatic characterization of each unit. Finally, the relationships between clusters were traced using a hierarchical plot-based classification. Results: Fifteen clusters were obtained, 13 natural and two artificial, of which two represented the sub-pa´ramo, nine the mid-pa´ramo and four the super-pa´ramo. Even though data representativeness was a potential limitation to segregate certain subpa ´ramo and super-pa´ramo units, the overall bioregionalisation was robust and represented important latitudinal, altitudinal and climatic gradients. Discussion: This study is the first to bioregionalise the pa´ramo province based on a substantial widely distributed biological dataset, and therefore provides important novel scientific insight on its biogeography. The obtained phytogeographical units can be used to support further research on the pa´ramo at smaller scale and on the humid Neotropical high-elevation ecosystems at broader-scale. Finally, several units were highlighted in our results as particularly worthy of further scientific and conservation focus.

This study explores 9 different biomaterials (agro-wastes) and inert materials, to be used as support in SSF to produce sophorolipids (SLs) with Starmerella bombicola ATCC 22214. Winterization oil filtration cake and sugar beet molasses were the substrates. Biomaterials (lignocellulosic wastes or organic by-products) performed better than inert materials (perlite and polyurethane foams). High yields were obtained for rice husk (0.15 g SLs g −1 DM) and wheat straw (0.20 g SLs g −1 DM). Lactonic C18:1 predominates over acidic form in the mixture of SLs. The characterization of the process through a time course experiment shows that wheat straw is the best material to produce SLs. A proportional correlation between SLs yield and cumulative oxygen consumption was found, suggesting that cumulative oxygen content (COC) can be used as an indirect measure to monitor the SLs production. Graphic abstract

In recent years, the number of articles reporting the addition of nanomaterials to enhance the process of anaerobic digestion has exponentially increased. The benefits of this addition can be observed from different aspects: an increase in biogas production, enrichment of methane in biogas, elimination of foaming problems, a more stable and robust operation, absence of inhibition problems, etc. In the literature, one of the current focuses of research on this topic is the mechanism responsible for this enhancement. In this sense, several hypotheses have been formulated, with the effect on the redox potential caused by nanoparticles probably being the most accepted, although supplementation with trace materials coming from nanomaterials and the changes in microbial populations have been also highlighted. The types of nanomaterials tested for the improvement of anaerobic digestion is today very diverse, although metallic and, especially, iron-based nanoparticles, are the most frequently used. In this paper, the abovementioned aspects are systematically reviewed. Another challenge that is treated is the lack of works reported in the continuous mode of operation, which hampers the commercial use of nanoparticles in full-scale anaerobic digesters.

A thorough literature review was conducted to investigate the behaviour of food waste in anaerobic digestion experiments. The main goal of this literature review was to study the effect of several operating parameters on methane yields and to develop a simplified regression equation to predict methane generation. Using a data prospection methodology, all the papers published within 2013–2015 that contained selected keywords were included in this study (a total of 613 papers). After screening, 167 papers were finally retrieved using the search engines and our methodology. From these papers, data from 231 experiments were recorded and evaluated. The parameters recorded in each paper were: operation mode (batch or continuous), temperature (mesophilic or thermophilic), moisture content (wet or dry), presence or absence of pretreatment, reactor scale (laboratory, bench, pilot, demonstration/full scale), presence or absence of co-substrates (co- or mono-digestion), organic loading rate, hydraulic retention time (HRT) and methane yield. The novelty of the work is that it employed various statistical tools to examine the effect of the above-mentioned factors on food waste methane generation. Most of the experiments were performed at mesophilic temperatures, at a wet system without substrate pretreatment. An equal number of papers described mono-digestion and co-digestion studies, and an equal number of papers described batch and continuous reactor experiments. The mean HRT for the continuous processes was 36.7 days. Statistical analysis indicated that the parameters that significantly affected methane yields were the “operation mode” and “pretreatment”. A best reduced regression model was fitted to the methane yield data to describe the above effects. As a general conclusion, with this methodology, that involved the analysis of a large number of studies (with different conditions and set-ups, heterogeneous waste, etc.), correlations between some typical operating parameters of anaerobic digestion and methane yields were not obvious.

Solid-state fermentation (SSF) is, by definition, a technology carried out in absence or near absence of free water. Therefore, it allows the use of solid materials as substrates for further biotransformation. SSF has gained attention in the last years being reported as a promising eco-technology that allows obtaining bioproducts of industrial interest using solid biomass (wastes and by-products). Main advantages over conventional submerged fermentation rely on the lower water and energy requirements, which generate minimum residual streams. However, drawbacks related to poor homogeneity and energy and mass transfer often appear, hindering the process yield and the downstream of the produced bioproducts. Despite the difficulties, many successful processes have been reported on the production of a variety of bioproducts such as hydrolytic enzymes, mostly carbohydrases for bioethanol production, and to a lesser extent, aromas, biosurfactants, biopesticides, bioplastics, organic acids or phenolic compounds. Most of the reported research focuses on process development at small scale; however, the main challenges to overcome in SSF are related to the upscaling and the development of a consistent and continuous operation. In this work, the main advances for the production of valuable/innovative bioproducts are presented and discussed.

The graphical abstract of this study. The use of alternative feedstocks such as industrial or food waste is being explored for the sustainable production of sophorolipids (SLs). Microbial biosurfactants are mainly produced via submerged fermentation (SmF); however, solid-state fermentation (SSF) seems to be a promising alternative for using solid waste or byproducts that could not be exploited by SmF. Applying the advantages that SSF offers and with the aim of revalorizing industrial organic waste, the impact of carbon and nitrogen sources on the relationship between yeast growth and SL production was analyzed. The laboratory-scale system used winterization oil cake as the solid waste for a hydrophobic carbon source. Pure hydrophilic carbon (glucose) and nitrogen (urea) sources were used in a Box–Behnken statistical design of experiments at different ratios by applying the response surface methodology. Optimal conditions to maximize the production and productivity of diacetylated lactonic C18:1 were a glucose:nitrogen ratio of 181.7:1.43 (w w −1 based on the initial dry matter) at a fermentation time of 100 h, reaching 0.54 total gram of diacetylated lactonic C18:1 with a yield of 0.047 g per gram of initial dry mass. Moreover, time course fermentation under optimized conditions increased the SL crude extract and diacetylated lactonic C8:1 production by 22% and 30%, respectively, when compared to reference conditions. After optimization, industrial wastes were used to substitute pure substrates. Different industrial sludges, OFMSW hydrolysate, and sweet candy industry wastewater provided nitrogen, hydrophilic carbon, and micronutrients, respectively, allowing their use as alternative feedstocks. Sweet candy industry wastewater and cosmetic sludge are potential hydrophilic carbon and nitrogen sources, respectively, for sophorolipid production, achieving yields of approximately 70% when compared to the control group.

1. Although invasions by alien plants are major threats to the biodiversity of natural habitats, individual habitats vary considerably in their susceptibility to invasion. Therefore the risk assessment procedures, which are used increasingly by environmental managers to inform effective planning of invasive plant control, require reliable quantitative information on the extent to which different habitats are susceptible to invasion. It is also important to know whether the levels of invasion in different habitats are locally specific or consistent among regions with contrasting climate, flora and history of human impact. 2. We compiled a database of 52 480 vegetation plots from three regions of Europe: Catalonia (Mediterranean-submediterranean region), Czech Republic (subcontinental) and Great Britain (oceanic). We classified plant species into neophytes, archaeophytes and natives, and calculated the proportion of each group in 33 habitats described by the European Nature Information System (EUNIS) classification. 3. Of 545 alien species found in the plots, only eight occurred in all three regions. Despite this large difference in species composition, patterns of habitat invasions were highly consistent between regions. None or few aliens were found in environmentally extreme and nutrient-poor habitats, e.g. mires, heathlands and high-mountain grasslands. Many aliens were found in frequently disturbed habitats with fluctuating nutrient availability, e.g. in man-made habitats. Neophytes were also often found in coastal, littoral and riverine habitats. 4. Neophytes were found commonly in habitats also occupied by archaeophytes. Thus, the number of archaeophytes can be considered as a good predictor of the neophyte invasion risk. However, neophytes had stronger affinity to wet habitats and disturbed woody vegetation while archaeophytes tended to be more common in dry to mesic open habitats. 5. Synthesis and applications. The considerable inter-regional consistency of the habitat invasion patterns suggests that habitats can be used as a good predictor for the invasion risk assessment. This finding opens promising perspectives for the use of spatially explicit information on habitats, including scenarios of future land-use change, to identify the areas of highest risk of invasion.