Catalogue Search | MBRL
Are you sure you want to remove the book from the shelf?
{{itemTitle}}
45
result(s) for
"Foster, Evelyn"
Sort by:
Alan and the animals
Alan, who loves all sorts of animals, has everything from ten rats to ten wild dogs.
Book
Computational Simulation Expands Understanding of Electrotransfer-Based Gene Augmentation for Enhancement of Neural Interfaces
The neural interface is a critical factor in governing efficient and safe charge transfer between a stimulating electrode and biological tissue. The interface plays a crucial role in the efficacy of electric stimulation in chronic implants and both electromechanical properties and biological properties shape this. In the case of cochlear implants, it has long been recognized that neurotrophins can stimulate growth of the target auditory nerve fibers into a favorable apposition with the electrode array, and recently such arrays have been re-purposed to enable electrotransfer (electroporation)-based neurotrophin gene augmentation to improve the \"bionic ear.\" For both this acute bionic array-directed electroporation and for chronic conventional cochlear implant arrays, the electric fields generated in target tissue during pulse delivery are central to efficacy, but are challenging to map. We present a computational model for predicting electric fields generated by array-driven DNA electrotransfer in the cochlea. The anatomically realistic model geometry was reconstructed from magnetic resonance images of the guinea pig cochlea and an eight-channel electrode array embedded within this geometry. The model incorporates a description of both Faradaic and non-Faradaic mechanisms occurring at the electrode-electrolyte interface with frequency dependency optimized to match experimental impedance spectrometry measurements. Our simulations predict that a tandem electrode configuration with four ganged cathodes and four ganged anodes produces three to fourfold larger area in target tissue where the electric field is within the range for successful gene transfer compared to an alternate paired anode-cathode electrode configuration. These findings matched
transfection efficacy of a green fluorescent protein (GFP) reporter following array-driven electrotransfer of the reporter-encoding plasmid DNA. This confirms utility of the developed model as a tool to optimize the safety and efficacy of electrotransfer protocols for delivery of neurotrophin growth factors, with the ultimate aim of using gene augmentation approaches to improve the characteristics of the electrode-neural interfaces in chronically implanted neurostimulation devices.
Journal Article
Mobile Devices and Augmented Reality: Psychomotor Skills Instruction in Emergency Medical Services Education
The use of wireless mobile devices (WMDs) with augmented reality (AR) in emergency medical services (EMS) education was a viable method to supplement psychomotor skill instruction. Current methods were expensive, time consuming, and inefficient. The overall result was poor skill competence and compromises in patient safety. However, recent changes made by the National Registry of EMTs (NREMT) tried to address this issue through increased emphasis on documenting the development of skill competency. Unfortunately, there were no suggestions for instructional methodologies leaving instructors to struggle with developing skill competency. Literature review results indicated active learning methods were best but EMT programs were constrained in implementation by lack of resources. One possible solution was the use of WMDs with AR as a low-cost method of providing active learning activities. Accordingly, the purpose of this quasi-experimental, quantitative study was to examine the efficacy of WMDs with AR as an adjunct to psychomotor skill instruction for manual ventilation and trauma assessment. The study was guided by the constructivism learning theory. Participants were EMT students at a university and community college in Western North Carolina. These students were given access to MedrillsTM, an AR application for instruction of EMT skills, as part of traditional skill instruction. Following instruction, student performance on skill exams for manual ventilation and trauma assessment were evaluated with NREMT skill check sheets. The results for each skill were compared to previous students who did not have the application using the t-test for independent means. Surprisingly, there were no statistically significant differences in the skill competency of the two groups. However, the sample size was small and the number of skills limited so a larger research study is needed. The conclusion was instruction with WMDs with AR was just as effective as traditional methods of instruction for manual ventilation and trauma assessment. Unfortunately, the cost was prohibitive so it should only be used in very limited situations. The findings of this study will be used by EMT instructors to determine if the methodology was a good fit for their EMT courses.
Dissertation
Letter: The BIG issue - Sad saga of Woolies' woe
WOOLWORTHS is on offer for just pounds 1 to anyone ready to take on its pounds 385million debt, putting 30,000 jobs at risk. The Mirror's Paul Routledge, once a Woolies Saturday boy, recalled its glory days.
Newspaper Article
Putting drama into learning
The basic skills agency is concerned about children's communication. Ninety per cent of nursery workers are worried about the speech skills of pre-schoolers. Meanwhile some young pupils starting school are struggling to express themselves. More five-year-olds than ever have difficulties with reading and speech. Many believe over-exposure to poor quality TV and electronic gadgets is having a negative effect on children's language.
Magazine Article
X-ray crystallographic studies of cytoplasmic quinone reductases involved in cellular detoxification
Detoxifying enzymes form one component of cellular response to oxidative and electrophilic stress. Levels of detoxifying enzymes are coordinately regulated in response to low concentrations of a large number of structurally diverse compounds, and increases in detoxifying enzyme levels are associated with increases in the antioxidant status of the cell and/or detoxification of metabolites. NAD(P)H:quinone reductase type 1 (QR1) is a cytoplasmic flavoenzyme which functions to detoxify exogenous quinones. In mammals, a second isoform of QR1 has been identified and is now known as QR2. Like QR1, QR2 catalyzes two-electron reductions of quinones but exhibits distinct biochemical properties. In attempts to explore the function of QR2 we have determined the three-dimensional structure of human QR2, alone and in complexes with substrates, by X-ray diffraction. This high-resolution structure points to a conserved mechanism between QR1 and QR2. A second line of inquiry investigated the peculiar phylogenetic distribution of QR1, which is represented among mammals, bony fish, primitive eukaryotes, bacteria, and viruses, but not, for example, in yeast, C. elegans , or D. melanogaster. Very few of the non-mammalian homologs have been characterized, and no structural information is available. The QR1 homolog of the unicellular eukaryote Giardia lamblia has been characterized and shown to have catalytic properties very similar to mammalian QR1. Crystallization and preliminary X-ray diffraction studies of Giardia lamblia QR1 are reported.
Dissertation
Protest Against Plan to Slaughter Wild Life for Food
It is with a deep sense of disappointment that we learn through the press that Dr. Grenfell has returned to this country from England and is now on the way to Labrador with the avowed purpose and the means to establish there extensive deer ranches for the systematic breeding and slaughter of wild deer.
Newspaper Article