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Adsorption Studies of Salmonella Enteritidis and Escherichia coli on Chitosan-Coated Magnetic Nanoparticles
One of the challenges of microbiological testing is the complex and lengthy sample preparation, causing delays in getting the final result. Immunomagnetic separation is one of the sample preparation techniques recently used to overcome this complexity. However, it is expensive, fragile, and requires cold storage. This study aimed to use chitosan-coated magnetic nanoparticles (cMNP) to capture bacterial cells from a simulated matrix and understand the interaction between the bacteria and the cMNP using batch adsorption studies. To illustrate the concept, Salmonella Enteritidis and Escherichia coli were used. Results showed that the adsorption of Salmonella Enteritidis and E. coli fitted the pseudo-second-order kinetic model (R2 = 0.939 and 0.968, respectively) and the Freundlich isotherm model (R2 = 0.999 and 0.970, respectively). The increased ionic strength enhanced bacterial adsorption, and the highest capture efficiency was observed at pH 4 (32.8% and 98.1% for Salmonella Enteritidis and E. coli, respectively). These results show that chemisorption plays a significant role in bacterial adsorption to cMNP. Furthermore, increasing ionic strength and acidic pH (pH 4) significantly affects the adsorption of Salmonella Enteritidis and E. coli on cMNP, making them crucial for enhancing the performance of cMNP-based sample preparation methods.
Journal Article
Field Guide: Morphometric Visualization and Characterization of Selected Foodborne Pathogens Using Advanced Imaging Techniques
This paper aims to present the phenotypic characteristics, such as length, width, circular diameter, volume, surface area to cross-sectional area ratio, surface uniformity, and surface texture, of the foodborne pathogens Salmonella enterica serovar Agona, Salmonella enterica serovar Enteritidis, Listeria monocytogenes, and Campylobacter jejuni. It is a novel “field-guide” presentation of the observable morphological characteristics of these four species in the form of a Dichotomous Key. Phenotypic values of the pathogens were measured using a high-accuracy, nano-level-resolution 3D laser confocal scanning microscope. All samples were fixed with glutaraldehyde and stained with methylene blue, ensuring morphological preservation. The images of the pathogens were captured under various imaging modes, including 3D, laser, and transmission electron microscopy resolutions. Results show that these pathogens exhibit distinct morphological and surface properties, with Campylobacter jejuni showing unique features. The dichotomous key shows a simplified way of classifying selected foodborne pathogens from their morphometric properties. The morphometric measurements, in many instances first reported in the literature, and images provide a readily observable way to identify and classify microorganisms, allowing researchers to potentially study evolutionary relationships, assess species diversity, and understand how organisms interact with their environment, especially when genetic information is limited or difficult to obtain.
Journal Article
On Story—Screenwriters and Filmmakers on Their Iconic Films
“On Story is film school in a box, a lifetime’s worth of filmmaking knowledge squeezed into half-hour packages.\"—Kenneth Turan, film critic for the Los Angeles Times Austin Film Festival (AFF) is the first organization focused on the writer’s creative contribution to film. Its annual Film Festival and Conference offers screenings, panels, workshops, and roundtable discussions that help new writers and filmmakers connect with mentors and gain advice and insight from masters, as well as refreshing veterans with new ideas. To extend the festival’s reach, AFF produces On Story, a television series currently airing on PBS-affiliated stations and streaming online that presents footage of high-caliber artists talking candidly and provocatively about the art and craft of screenwriting and filmmaking, often using examples from their own films. On Story—Screenwriters and Filmmakers on Their Iconic Films presents renowned, award-winning screenwriters and filmmakers discussing their careers and the stories behind the production of their iconic films such as L.A. Confidential, Thelma & Louise, Groundhog Day, Guardians of the Galaxy, The Silence of the Lambs, In the Name of the Father, Apollo 13, and more. In their own lively words transcribed from interviews and panel discussions, Ron Howard, Callie Khouri, Jonathan Demme, Ted Tally, Jenny Lumet, Harold Ramis, and others talk about creating stories that resonate with one’s life experiences or topical social issues, as well as how to create appealing characters and bring them to life. Their insights, production tales, and fresh, practical, and proven advice make this book ideal for film lovers, screenwriting students, and filmmakers and screenwriters seeking inspiration.
eBook
The Deer
This 40th anniversary issue edited by best-selling novelist Alice Hoffman (The Dovekeepers, Practical Magic) features new work from Ursula K. Le Guin, Marge Piercy, Jennifer Haigh, Ann Hood, and Wally Lamb; an interview with Elizabeth Bishop from the archives; and a story from Ploughshares ' first Emerging Fiction Writer's Award winner Thomas Lee.
Journal Article
The shower and the gory
What made James Franco dress up as Janet Leigh and recreate her murder in Psycho? Here, the actor explains the thinking behind his latest artwork Psycho Nacirema. (Quotes from original text)
Journal Article
JAMES FRANCO ON SCHOOL
Mentors. I’ve had a few. I’ve been to too many schools. I’ve been at some schools for too much time. I’ve been a mentor to some. But I haven’t given enough time to being a mentor; this is why I teach at two universities, one art school, and have a bicoastal acting and filmmaking school of my own.
The teachers I loved—acting teachers, writing teachers, directing teachers—were the ones who were completely focused on their students. There were some teachers who had their own careers, and you knew that they were only doing it for the money. Some
Book Chapter
Foreword
When I played Saul in Pineapple Express I said, fuck it, acting should be fun. No more twisted, self-centered, James Dean demons for me, just go with the flow.
There was one thing that was important: Saul should love Dale. That was the secret that made Saul so much more than just another dealer in another stoner comedy. He really cared about something, and that made the audience care about him. In this case he cared about Seth’s character, just like I care about Seth.
Then I played Scott Smith, Harvey Milk’s lover. I’m still surprised by the response to
Book Chapter
William T. Vollmann
\"This fascinating, massive, wide-ranging collection that editors Christopher K.Coffman and Daniel Lukes have gathered together into William T.Vollmann: A Critical Companion will soon be recognized as one of those rare critical books for which that egregiously overused term 'groundbreaking' is fully justified.\" --Larry McCaffery, from the preface.
eBook
Howl
James Franco delivers a career-defining performance as poet Allen Ginsberg. Through his search for personal and creative expression, Ginsberg writes Howl, and must contend with attorney Jake Ehrlich (Jon Hamm) during Howl's bizarre obscenity trial.
Streaming Video