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To replicate inside macrophages and cause tuberculosis, Mycobacterium tuberculosis must scavenge a variety of nutrients from the host 1 , 2 . The mammalian cell entry (MCE) proteins are important virulence factors in M. tuberculosis 1 , 3 , where they are encoded by large gene clusters and have been implicated in the transport of fatty acids 4 – 7 and cholesterol 1 , 4 , 8 across the impermeable mycobacterial cell envelope. Very little is known about how cargos are transported across this barrier, and it remains unclear how the approximately ten proteins encoded by a mycobacterial mce gene cluster assemble to transport cargo across the cell envelope. Here we report the cryo-electron microscopy (cryo-EM) structure of the endogenous Mce1 lipid-import machine of Mycobacterium smegmatis —a non-pathogenic relative of M. tuberculosis . The structure reveals how the proteins of the Mce1 system assemble to form an elongated ABC transporter complex that is long enough to span the cell envelope. The Mce1 complex is dominated by a curved, needle-like domain that appears to be unrelated to previously described protein structures, and creates a protected hydrophobic pathway for lipid transport across the periplasm. Our structural data revealed the presence of a subunit of the Mce1 complex, which we identified using a combination of cryo-EM and AlphaFold2, and name LucB. Our data lead to a structural model for Mce1-mediated lipid import across the mycobacterial cell envelope. Proteins of the Mycobacterium smegmatis Mce1 system assemble to form an elongated ABC transporter complex that is long enough to span the impermeable mycobacterial cell envelope.

Mycobacterium tuberculosis (Mtb) infects human macrophages, where it scavenges nutrients for survival. The Mammalian Cell Entry (MCE) proteins are important virulence factors implicated in import of nutrients such as fatty acids from the host, but their structures and mechanisms remain unknown. Here we report the high-resolution structure of the endogenous Mce1 transporter from Mycobacterium smegmatis, a non-pathogenic relative of Mtb. Ten distinct proteins assemble into an elongated complex, long enough to span the cell envelope. A unique helical needle creates a curved hydrophobic tunnel for lipid transport across the periplasm. Combining cryo-EM and AlphaFold2, we identify a previously unknown subunit of the Mce1 complex, which we name LucB. Our data lead to a structural model for Mce1-mediated fatty acid import in mycobacteria.Competing Interest StatementThe authors have declared no competing interest.Footnotes* https://genome.med.nyu.edu/public/bhabhaekiertlabs/* https://www.rcsb.org/structure/8FEF* https://www.rcsb.org/structure/8FED* https://www.rcsb.org/structure/8FEE* http://massive.ucsd.edu/ProteoSAFe/QueryPXD?id=PXD038456* http://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD038456* https://www.ebi.ac.uk/empiar/EMPIAR-111343/