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This book comprehensively explores the characteristics and expressions of low-velocity and high-velocity nonlinear flow infiltration in porous media, offering evaluation methods through mathematical models and analytical solutions, with a focus on applications in oil and gas development, reservoir properties, and fluid dynamics.

In order to achieve sustainable development of mature oilfield, a series of adjustment measures should be implemented to improve production performance at the extra-high water cut stage. South Kumkol reservoir is a typical multi-layer low viscosity oil reservoir, which has the characteristics of small sandstone body, high shale volume, and strong heterogeneity. At present, the water cut of the South Kumkol reservoir is about 90%, which is on the verge of being abandoned. Multi-layer cyclic alternate injection and production (MCA-IP) is an ideal adjustment measure for multi-layer oil reservoir to improve oil recovery (IOR) at the extra-high water cut stage. In this paper, we designed the double-plate visual physical device and the MCA-IP experimental program and then calculated the sweep coefficient using image recognition method. Furthermore, the sweep coefficient was quantitatively calculated by image recognition method. The results show that the sweep area extends to both sides of the main streamline and the sweep efficiency is gradually improved after the completion of MCA-IP. In addition, the IOR mechanism of MCA-IP mainly includes reperforation, well-pattern encryption, and asynchronous injection-production. The reperforation and well-pattern encryption increased the sweep coefficient by about 19.52%, while asynchronous injection-production increased the sweep coefficient by about 1.2%, and the overall sweep coefficient increased by about 20.7%. According to the experimental data statistics, the MCA-IP method can increase oil recovery by about 11% and reduce water cut by about 6%.

Background The study describes the clinical manifestations and variant screening of two Chinese siblings with primary ciliary dyskinesia (PCD). They carry the same DNAAF2 genotype, which is an extremely rare PCD genotype in the Chinese population. In addition, the study illustrated an overview of published variants on DNAAF2 to date. Methods A two-child family was recruited for the study. Clinical manifestations, laboratory tests, bronchoscopic and otoscopic images, and radiographic data were collected. Whole blood was collected from siblings and their parents for whole-exome sequencing (WES) and Sanger sequencing to screen variants. Results The two siblings exhibited typical clinical manifestations of PCD. Two compound heterozygous variants in DNAAF2 were detected in both by WES. Nonsense variant c.156 C>A and frameshift variant c.177_178insA, which was a novel variant. Conclusion The study identified a novel variant of DNAAF2 in Chinese children with a typical phenotype of PCD, which may enrich our knowledge of the clinical, diagnostic and genetic information of DNAAF2-induced PCD in children.

Capacitance–resistance models (CRMs) are semi-analytical methods to estimate the production rate of either an individual producer or a group of producers based on historical observed production and injection rates using material balance and signal correlations between injectors and producers. Waterflood performance methods are applied to evaluate the waterflooding performance effect and to forecast the development index on the basis of Buckley–Leverett displacement theory and oil–water permeability curve. In this case study, we propose an approach that combines a capacitance–resistance model (CRM) modified by increasing the influence radius on the constraints and a waterflood performance equation between oil cut and oil accumulative production to improve liquid and oil production prediction ability. By applying the method, we can understand the waterflood performance, inter-well connectivities between injectors and producer, and production rate fluctuation better, in order to re-just the water injection and optimize the producers’ working parameters to maximize gain from the reservoir. The new approach provides an effective way to estimate the conductivities between wells and production rates of a single well or well groups in CRMs. The application results in Kalamkas oilfield show that the estimated data can be in good agreement with the actual observation data with small fitting errors, indicating a good development index forecasting capability.

Three-dimensional (3D) seismic data and well log data were used to investigate the sandstone architecture of the Middle Jurassic deltaic reservoirs of the Zhetybay Oilfield, Mangeshrak Basin, Kazakhstan. The base-level cycles of different scales were identified and divided using well log and 3D seismic data. Five types of sedimentary boundaries were identified in the mouth bar sandstones. The boundaries divide single mouth bars. Vertically, the spatial distribution of sand bodies can be divided into superposed, spliced, and isolation modes. Laterally, contact modes can be divided into superposition, lateral, and isolation modes. We found that the base-level cycle controls the evolution of the delta front sand body architecture. In the early decline or late rise of the base-level cycle, the superimposed or spliced modes dominate the sand body. By contrast, the lateral or isolation modes dominate the sand body in the late decline or early rise of the base-level cycle. This paper proposes an architecture model of the delta front sand bodies controlled by the base-level cycle. The spatial distribution and morphological variation of deltaic sand bodies could be linked to the base-level cycles.

Rare autosomal-recessive variants in tetratricopeptide repeat domain 7A ( TTC7A ) gene have been shown to cause intestinal and immune disorders of variable severity. Missense mutations in TTC7A gene, usually retaining most of the functional motifs, is associated with relative milder clinical presentations. In this study, we reported a patient who was suffering from severe multiple intestinal atresia (MIA) with combined immunodeficiency (CID) that led to the pyloric diaphragm, ileum atresia, colon stenosis, and multiple episodes of sepsis. In spite of several surgeries and supportive treatment, the patient died of severe sepsis and multiple organ failure at age of 3 months. The whole exome sequencing (WES) of peripheral blood samples identified a novel homozygous TTC7A missense mutation (c. 206T>C, p. L69P), inherited from his parents with consanguineous marriage. In silico analysis revealed that a hydrogen bond present between Gly65 and Leu69 in the wild-type TTC7A was disrupted by the Leu69Pro mutation. Moreover, this homozygous missense mutation led to a reduced TTC7A expression in lymphocytes and intestinal tissues, accompanied by impeded lymphocyte development. Further studies demonstrated that the PI4K-FAM126A-EFR3A pathway was impaired in colon tissues. Our data strongly support the linkage of severe MIA-CID with the missense mutation in TTC7A gene. More knowledge of the TTC7A protein functions will have important therapeutic implications for patients with MIA-CID.

Under the background that the in-depth profile control technology is gradually applied in low-permeability fractured reservoirs, this paper regards block H of Changqing Oilfield as the research object, referring to the range of its physical parameters and field application data. Three common in-depth profile control agents (PCAs), nanosphere suspension, poly(ethylene glycol) single-phase gel particle (PEG) and cross-linked bulk gel and swelling particle (CBG-SP), are selected to investigate the compatibility between the fractured channels and the PCAs through a series of experiments. The experimental results show that the nanospheres with particle sizes of 100 nm and 300 nm have good injectivity and deep migration ability, which remains the overall core plugging rate at a high level. The residual resistance coefficient of 800 nm nanospheres decreases in a “cliff” manner along the injection direction due to the formation of blockage in the front section, resulting in a very low plugging rate in the rear section. The injection rate is an important parameter that affects the effect of PEG in the fractured channels. When the injection rate is lower than 0.1 mL/min, the plugging ability will be weakened, and if the injection rate is higher than 0.2 mL/min, the core plugging will occur. The appropriate injection rate will promote the better effect of PEG with the plugging rate > 90%. The average plugging rate of CBG-SP in fractured rock core is about 80%, and the overall control and displacement effect is good. Based on the experimental data of PCAs, the optimization criteria of slug configuration and pro-duction parameters are proposed. According to the principle “blocking, controlling and displacing”, references are provided for PCAs screening and parameters selection of field implementation.

Weak gel is a gel system formed by the mixing and crosslinking of a low-concentration polymer and a slow-release crosslinker. It can be used for profile control in deep reservoir, but its effect is greatly affected by mechanical shearing. Currently, the shearing effect on weak gel is mainly studied by way of mechanical stirring, while the effect of porous media shear on weak gel molecules and properties has been rarely discussed. In this paper, polymer solution, aluminum gel and phenolic gel were prepared. The molecular coil size, viscoelastic modulus and microscopic aggregation morphology in water solution of three systems before and after core shearing were investigated, and the injection performance of the three systems in cores with different permeabilities was tested by physical simulation experiments. The study results show that at equivalent permeability, the system with a larger equivalent sphere diameter of molecular coil is more seriously sheared and suffers greater viscosity loss. In the core with permeability of 1.0 D, polymer solution remains as the aggregation, while phenolic gel and aluminum gel cannot form network aggregations and they are inferior to polymer solution in migration capacity in the mid-deep part of the core. In the core with permeability of 1–5.8 D, the polymer solution remains as a Newtonian fluid, while phenolic gel and aluminum gel become purely viscous non-Newtonian fluids. The elastic modulus of aluminum gel and phenolic gel is four times more than that of a polymer. In the core with permeability higher than 8.5 D, aluminum gel and phenolic gel migrate with less effect by core shearing, and their profile control capacity in deep reservoir is higher than that of the polymer. In the core with permeability lower than 8.5 D, because the monomolecular activity of weak gels becomes poor, they migrate in porous media with more effect by core shearing, and their profile control and oil displacement capacity in deep reservoir is lower than that of the polymer.

Different light-based strategies have been investigated to inactivate viruses. Herein, we developed an HIV-based pseudotyped model of SARS-CoV-2 (SC2) to study the mechanisms of virus inactivation by using two different strategies; photoinactivation (PI) by UV-C light and photodynamic inactivation (PDI) by Photodithazine photosensitizer (PDZ). We used two pseudoviral particles harboring the Luciferase-IRES-ZsGreen reporter gene with either a SC2 spike on the membrane or without a spike as a naked control pseudovirus. The mechanism of viral inactivation by UV-C and PDZ-based PDI were studied via biochemical characterizations and quantitative PCR on four levels; free-cell viral damage; viral cell entry; DNA integration; and expression of reporter genes. Both UV-C and PDZ treatments could destroy single stranded RNA (ssRNA) and the spike protein of the virus, with different ratios. However, the virus was still capable of binding and entering into the HEK 293T cells expressing angiotensin-converting enzyme 2 (ACE-2). A dose-dependent manner of UV-C irradiation mostly damages the ssRNA, while PDZ-based PDI mostly destroys the spike and viral membrane in concentration and dose-dependent manners. We observed that the cells infected by the virus and treated with either UV-C or PDZ-based PDI could not express the luciferase reporter gene, signifying the viral inactivation, despite the presence of RNA and DNA intact genes.