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Phytochemicals have been effectively used to enhance the growth and productivity of farm animals, while the potential roles of essential oils and their nano-emulsions are limited. This plan was proposed to investigate the impacts of orally administered moringa oil (MO) or its nano-emulsion (NMO) on the growth, physiological response, blood health, semen attributes, and sperm antioxidant-related genes in rams. A total of 15 growing Rahmani rams were enrolled in this study and allotted into three groups. The 1 st control group received a basal diet only and treated orally one mL of distilled water, while the 2 nd , and 3 rd groups received a basal diet and were orally treated with 1 mL of NMO or 2 mL of MO /head/day for 4 months, respectively. Growth, physiological response, blood health, semen quality, and antioxidant genes in sperm were assessed. The MO and NMO treatments had no significant effect on growth indices (final body weight and weight gain ) and physiological response (rectal temperature, pulse, and respiration rates) ( P  > 0.05). The NMO group had the lowest levels of MCV (mean corpuscular volume) ( P  < 0.05), while all treated groups produced higher levels of mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) compared to those in the control group ( P  < 0.05). Aspartate transferase (AST) and total cholesterol were significantly reduced in the MO and NMO groups, while total protein and glucose levels were significantly improved in NMO group ( P  < 0.05). Serum and seminal interstitial-cell-stimulating hormone (ICSH) levels were significantly improved ( P  < 0.0001) in the NMO group. Testosterone in serum and seminal plasma was significantly improved ( P  < 0.0001) in the MO group. Total antioxidant capacity (TAC) levels showed a tendency to increase in both the MO and NMO groups, but this increase was not significant compared to the untreated group ( P  > 0.05). On the other hand, the MO group exhibited lower levels of AST and malondialdehyde (MDA), while the alanine aminotransferase (ALT) levels were the lowest in the NMO group ( P  > 0.05). Mass motility, viability, membrane integrity and sperm concentration were significantly improved in the MO group ( P  < 0.0001) compared to the other groups. The NMO group had worse expressions of superoxide dismutase 1 (SOD1) compared to the control and MO groups. MO group significantly upregulated the catalase gene compared to the other groups ( P  < 0.001). The expression of Caspase-3 was highest in the group that received NMO compared to the other groups ( P  < 0.001). This study suggests that MO may serve as a novel therapeutic agent for improving the reproductive health in Rahmani rams.

Recently, nanotechnology approaches have been employed to enhance the solubility, availability, and efficacy of phytochemicals, overcoming some industrial obstacles and natural biological barriers. In this regard, 120 clinically healthy growing V-line rabbits (5 weeks old) reared during the summer season were divided randomly into four equal experimental groups (30 rabbits each). The first group received a basal diet without the supplementation of the nanoemulsion of cardamom essential oil (NCEO) (0 g/kg diet) and served as a control (NCEO 0). The other groups were given diets containing NCEO at levels of 150 (NCEO 150), 300 (NCEO 300), and 600 (NCEO 600) mg/kg diet, respectively. The growth performance (higher LBW and ADG), feed utilization (lower FCR), dressing percentage, and relative weight of the liver were improved significantly in the NCEO-treated groups compared to the control group. Moreover, the dietary treatment significantly decreased the rectum temperature and respiration rate, minimizing the 350 and 325 mg NECO/kg diets. The erythrocyte count, hematocrit, and hemoglobin concentration were significantly increased (p < 0.05), while white blood cells were significantly diminished (p = 0.0200) in the NCEO300 and NCEO600 groups compared to the control group. Treatment with 300 or 600 mg NCEO/kg significantly increased the blood serum total protein and albumin compared to the control group. Meanwhile, the liver enzymes (AST and ALT), uric acid, and creatinine concentrations decreased significantly in the NCEO300 group compared to the control group. The concentrations of triglycerides and total cholesterol were reduced significantly by the dietary treatment. The total antioxidant capacity, dismutase activity, and glutathione concentration were significantly higher, while the malondialdehyde and protein carbonyl levels were significantly lower in the NCEO300 group than in the control. The inflammatory responses and immunity statuses were improved in the blood serum of the NCEO-treated rabbits compared to the control. Heat-stress-induced pathological perturbations in renal/hepatic tissues and NCEO co-treatment successfully re-established and recovered near-control renal–hepatic morphology. In conclusion, a dietary supplementation of NCEO (300 mg/kg) could effectively enhance growing rabbits’ growth indices, feed efficiency, redox balance, immunity, and inflammatory responses during the summer.

The study investigated the beneficial effects of vitamin E-loaded nano-emulsion (NEVE) on hemato-biochemical changes, oxidative stress, inflammatory responses, immunological variables, and semen quality in heat-stressed rabbit bucks. Forty adult V-line rabbit bucks were randomly assigned to four groups and fed diets containing NEVE at 0 (NEVE0), 50 (NEVE50), 100 (NEVE100), and 200 (NEVE200) mg/kg for 12 weeks. The NEVE had a mean particle diameter of 32.6 nm, a polydispersity index of 0.6, and a zeta potential of −31.1 mV. The dietary NEVE significantly improved the hematological, blood protein, lipid profile, and liver functions of rabbit bucks (p < 0.05). The NEVE200 treated group showed higher levels of nitric oxide, TAC, and SOD in blood serum and seminal plasma compared to the control group (p < 0.05). Meanwhile, levels of MDA, IL-4, and TNF-α were significantly shrunken in response to the dietary treatment (p < 0.0001), while the IgA and IgG were improved in the NEVE100 and NEVE200 groups. The NEVE200 exhibited higher progressive motility, ejaculate volume, viability, and better libido (lower reaction time) than the untreated group (p < 0.05). TEM images illustrated that NEVE200 exhibited higher sperm cells with a normal structure, intact plasma membranes, and acrosomes than the other groups. Moreover, testicular architecture was improved with NEVE supplementation. Overall, the dietary addition of NEVE at 100 or 200 mg/kg could be an effective strategy to improve the health and semen quality of rabbit bucks during hot climates.

This study was conducted to evaluate the effects of dietary Carnosic acid–chitosan nanoparticles (CA-CNPs) on growth performance, feed utilisation, and overall health status of heat-stressed growing rabbits. A total of 150 weaned New Zealand White rabbits (5 weeks old; 693.92 ± 15.42 g) were assigned to five groups: a control group fed a basal diet and four groups supplemented with 25, 50, 100, or 200 mg CA-CNPs/kg diet for eight weeks. The selected doses were based on a pilot study evaluating tolerance and preliminary growth responses. The temperature–humidity index (29.99 ± 0.82) indicated severe heat stress. Dietary CA-CNPs supplementation significantly improved body weight gain, feed conversion ratio, and performance index in a quadratic manner, with optimal responses observed at approximately 140 mg/kg diet. Rectal temperature and respiratory rate decreased quadratically, suggesting enhanced thermoregulation. Carcase yield and edible organ weights were significantly increased without affecting meat composition. CA-CNPs supplementation linearly increased serum total protein, albumin, globulin, thyroid hormones, immunoglobulins, and nitric oxide, while linearly reducing cholesterol, triglycerides, and glucose concentrations. Hepatic and renal biomarkers, MDA, 8–OHdG, and pro-inflammatory cytokines (TNF-α, IFN-γ, IL-6) decreased quadratically, whereas antioxidant enzymes (SOD, CAT, GPX) and total antioxidant capacity increased quadratically. Histological examination revealed marked hepatic and renal protection at 100 mg/kg. Quadratic regression analysis indicated optimal CA-CNPs inclusion levels between 125 and 140 mg/kg diet. In conclusion, dietary supplementation with CA-CNPs significantly improved growth performance, physiological adaptability, antioxidant defense, and immune function, while mitigating heat-induced oxidative and inflammatory stress in growing rabbits.

We report on the generation of white light from Ho-doped ZIF-8 nanocrystals synthesized by a simple wet chemical technique at room temperature. The influence of Holmium (Ho) concentration and heat treatment on both structural and optical responses has been investigated. The crystal structure and morphology of as-synthesized (As) and post-thermal annealed (TA) nanocrystals have been examined using X-ray diffraction (XRD) and Transmission Electron Microscope (TEM).  In addition, the thermal stability and bond structure have been studied via Thermogravimetric analysis (TGA) and Fourier Transform Infrared Spectroscopy (FTIR), respectively. Moreover, the photoluminescence (PL) measurements were performed using two excitation wavelengths: 445 nm and 355 nm. The XRD and TEM evidenced the sodalite nanocrystalline nature of the samples. The TGA and FTIR analyses demonstrated the degradation behavior of ZIF-8 due to the incorporation of Ho ions and thermal calcination. The PL results showed intense luminescence response, in particular, from the heat-treated samples. Furthermore, the PL exhibited a naked-eye observed white light emission. This was attributed to the structural modifications induced by the dopant and thermal treatment. Our findings could be used as a guideline towards the potential application of lanthanide-doped MOFs (ZIF-8) in light-emitting purposes.

The retina is highly sensitive to oxygen and blood supply, and hypoxia plays a key role in retinal diseases such as diabetic retinopathy (DR) and age-related macular degeneration (AMD). Müller glial cells, which are essential for retinal homeostasis, respond to injury and hypoxia with reactive gliosis, characterized by the upregulation of the glial fibrillary acidic protein (GFAP) and vimentin, cellular hypertrophy, and extracellular matrix changes, which can impair retinal function and repair. The retinal pigment epithelium (RPE) supports photoreceptors, forms part of the blood–retinal barrier, and protects against oxidative stress; its dysfunction contributes to retinal degenerative diseases such as AMD, retinitis pigmentosa (RP), and Stargardt disease (SD). Extracellular vesicles (EVs) play a crucial role in intercellular communication, protein homeostasis, and immune modulation, and have emerged as promising diagnostic and therapeutic tools. Understanding the role of extracellular vesicles’ (EVs’) signaling machinery of glial cells and the retinal pigment epithelium (RPE) is critical for developing effective treatments for retinal degeneration. In this study, we investigated the bidirectional EV-mediated crosstalk between RPE and Müller cells under hypoxic conditions and its impact on cellular metabolism and retinal cell integrity. Our findings demonstrate that RPE-derived extracellular vesicles (RPE EVs) induce time-dependent metabolic reprogramming in Müller cells. Short-term exposure (24 h) promotes pathways supporting neurotransmitter cycling, calcium and mineral absorption, and glutamate metabolism, while prolonged exposure (72 h) shifts Müller cell metabolism toward enhanced mitochondrial function and ATP production. Conversely, Müller cell-derived EVs under hypoxia influenced RPE metabolic pathways, enhancing fatty acid metabolism, intracellular vesicular trafficking, and the biosynthesis of mitochondrial co-factors such as ubiquinone. Proteomic analysis revealed significant modulation of key regulatory proteins. In Müller cells, hypoxic RPE-EV exposure led to reduced expression of Dyskerin Pseudouridine Synthase 1 (DKc1), Eukaryotic Translation Termination Factor 1 (ETF1), and Protein Ser/Thr phosphatases (PPP2R1B), suggesting alterations in RNA processing, translational fidelity, and signaling. RPE cells exposed to hypoxic Müller cell EVs exhibited elevated Ribosome-binding protein 1 (RRBP1), RAC1/2, and Guanine Nucleotide-Binding Protein G(i) Subunit Alpha-1 (GNAI1), supporting enhanced endoplasmic reticulum (ER) function and cytoskeletal remodeling. Functional assays also revealed the compromised barrier integrity of the outer blood–retinal barrier (oBRB) under hypoxic co-culture conditions. These results underscore the adaptive but time-sensitive nature of retinal cell communication via EVs in response to hypoxia. Targeting this crosstalk may offer novel therapeutic strategies to preserve retinal structure and function in ischemic retinopathies.

Nanopesticides have been recently introduced as novel pesticides to overcome the drawbacks of using traditional synthetic pesticides. The present study evaluated the acaricidal activity of Copper/Graphene oxide core-shell nanoparticles against two tick species, Rhipicephalus rutilus and Rhipicephalus turanicus . The Copper/Graphene oxide core-shell nanoparticles were synthetized through the solution plasma (SP) method under different conditions. The nanoparticles synthesized at 180 W and 45 min were highly toxic to Rh. rutilus and Rh. turanicus , with 50% lethal concentration (LC 50 ) values of 248.1 and 195.7 mg ml −1 , respectively, followed by those which were synthesized at 120 W/30 mins (LC 50  = 581.5 and 526.5 mg ml −1 ), 120 W/15 mins (LC 50  = 606.9 and 686.7 mg ml −1 ), and 100/45 mins (LC 50  = 792.9 and 710.7 mg ml −1 ), after 24 h of application. The enzyme assays revealed that 180 W/45 min treatment significantly inhibited the activity of acetylcholinesterase (115 ± 0.81 and 123 ± 0.33 U/ mg protein/min) and superoxide dismutase (290 ± 0.18 and 310 ± 0.92 U/ mg protein/min) in Rh. rutilus and Rh. turanicus , respectively, as compared with the negative control. The results also revealed a significantly increased catalase activity (895 ± 0.37 and 870 ± 0.31 U/ mg protein/min) in Rh. rutilus and Rh. turanicus , respectively. The above results indicated that Copper/Graphene oxide core-shell nanoparticles could be a promising alternatives for the management of ticks.

This study aimed to compare the efficacy of using a combination of antibiotic and corticosteroid eye drops to using the same combination in addition to sodium hyaluronate eye drops. This study included patients who underwent phacoemulsification for age-related cataract and presented to the ophthalmology department or outpatient clinic of the two hospitals in the study with DED during the period from January 2020 to January 2023. The study involved a two-pronged approach in which we retrospectively analyzed the prospectively maintained data of the patients and prospectively called the patients for an evaluation of the dry eye impact on vision-related function. The study patients were recruited from two hospitals. During the study period, patients who were treated with a combination of antibiotic and corticosteroid eye drops were enrolled in Group A, and those who were treated with the same combination with sodium hyaluronate eye drops were enrolled in Group B. The present study included 143 patients with post-phacoemulsification DED. Group A included 70 patients, and Group B included 73 patients. There was statistically significant improvement 4 weeks after treatment (p < 0.05) in the two groups. Comparing the two groups showed statistically significant improvements in group B compared to group A (p < 0.05). Assessment of the overall efficacy outcome revealed a statistically significant high percentage of cure (35.6% vs 1.4%) and high effectiveness (42.5% vs 13.6%) and a low percentage of effectiveness (21.9% vs 74.3%) and ineffectiveness (0.0% vs 5.7%) in Group B compared to Group A (p < 0.001). Combining sodium hyaluronate eye drops with tobramycin and dexamethasone eye drops yielded obviously better efficacy outcomes compared to using tobramycin and dexamethasone eye drops alone.

Waldenstrom’s Macroglobulinemia (WM) is an IgM-secreting bone marrow (BM) lymphoma that is preceded by an asymptomatic state (AWM). To dissect tumor-intrinsic and immune mechanisms of progression, we perform single-cell RNA-sequencing on 294,206 BM tumor and immune cells from 30 patients with AWM/WM, 26 patients with Smoldering Myeloma, and 23 healthy donors. Despite their early stage, patients with AWM present extensive immune dysregulation, including in normal B cells, with disease-specific immune hallmarks. Patient T and NK cells show systemic hypo-responsiveness to interferon, which improves with interferon administration and may represent a therapeutic vulnerability. MYD88 -mutant tumors show transcriptional heterogeneity, which can be distilled in a molecular classification, including a DUSP22 / CD9 -positive subtype, and progression signatures which differentiate IgM MGUS from overt WM and can help advance WM research and clinical practice. The impact of tumor intrinsic and immune alterations on disease progression in patients with Waldenstrom’s Macroglobulinemia (WM) remains to be characterized. Here, the authors perform single-cell RNA-sequencing and identify distinct tumor subtypes, tumour microenvironment features and potential therapeutic vulnerabilities in patients with WM.

This study characterized the mechanical behaviours of massive fractured (MLS), fossiliferous (FLS), and siliceous (SLS) limestone lithofacies under natural, dry, and saturated conditions. Uniaxial compressive strength (UCS), point load index (PLI), and indirect tensile strength (ITS) tests, along with petrographic, mineralogical, and geochemical analyses, were used to evaluate the impacts of the lithofacies composition and environmental conditions on rock strength. The results indicate that lithofacies composition, including mineralogy and texture, has a considerable effect on rock strength and durability. Under dry conditions, UCS values increased by up to ~ 200% in MLS and SLS relative to natural conditions, while saturation reduced UCS by 30–60% depending on lithofacies. Similar trends were observed in ITS, which decreased by up to 55% under saturation. The high silica content of SLS produced the most durable lithofacies, whereas the high porosity of FLS made it the most vulnerable to weakening from water exposure. MLS exhibited intermediate properties, as it loses strength considerably when existing fractures become saturated. Statistical analysis indicates that the CaO, SiO 2 , and MgO contents strongly influenced the rock mechanical properties. The study reveals relationships between lithofacies geochemistry, microstructural attributes (fractures, porosity, fossil interfaces), and mechanical responses under different moisture states. These insights allow for predictions about carbonate rock mechanical performance, making them crucial for geological research, engineering projects, industrial applications, and infrastructure design.