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Achieving magnetic bistability with large thermal hysteresis is still a formidable challenge in material science. Here we synthesize a series of isostructural chain complexes using 9,10-anthracene dicarboxylic acid as a photoactive component. The electron transfer photochromic Mn 2+ and Zn 2+ compounds with photogenerated diradicals are confirmed by structures, optical spectra, magnetic analyses, and density functional theory calculations. For the Mn 2+ analog, light irradiation changes the spin topology from a single Mn 2+ ion to a radical-Mn 2+ single chain, further inducing magnetic bistability with a remarkably wide thermal hysteresis of 177 K. Structural analysis of light irradiated crystals at 300 and 50 K reveals that the rotation of the anthracene rings changes the Mn1–O2–C8 angle and coordination geometries of the Mn 2+ center, resulting in magnetic bistability with this wide thermal hysteresis. This work provides a strategy for constructing molecular magnets with large thermal hysteresis via electron transfer photochromism. Achieving magnetic bistability with large thermal hysteresis is still a challenge in material science. Here, the authors report a Mn(II) chain complex that enables light-induced magnetic bistability with a 177 K thermal hysteresis loop.

Background Chronic non-healing wounds present a substantial economic burden to healthcare system; significant reductions in quality of life for those affected, and precede often serious events such as limp amputations or even premature deaths. This burden is also likely to increase with a larger proportion of elderly and increasing prevalence of life-style diseases such as obesity and diabetes. Reviews of the evidence on the burden of illness associated with chronic wounds have not been comprehensive in scope and have not provided an assessment of the distribution of the health care costs across categories of resource use. Methods/design This study is a systematic review of multiple databases for studies on adult patients with chronic wounds and with the primary objective to assess the impact on health-related quality of life by category of ulcers, and associated direct and indirect costs. Eligible studies will primary be empirical studies evaluating, describing or comparing measurement of quality of life and economic impact. Two reviewers will independently screen titles and abstracts and select studies involving adults with chronic wounds. These investigators will also independently extract data using a pre-designed data extraction form. Differences in applied methodologies and uncertainties will clearly be accounted for. Conservative valuations of costs and impact on health-related quality of life will be prioritised. Variations that may depend on age distribution, the categorisation of ulcer, healthcare system etc. will be described clearly. Discussion The proposed systematic review will yield a comprehensive assessment of the humanistic and economic burden of chronic wounds in an adult population. A better understanding of the humanistic and economic burden of chronic wounds is essential for policy and planning purposes, to monitor trends in disease burden and not at least in order to estimate the real-world cost-effectiveness of new treatments and therapies. Systematic review registration PROSPERO CRD42016037496

Background Chronic wounds impose a significant and often underappreciated burden to the individual, the healthcare system and the society as a whole. Preliminary literature search suggests that there are at present no reliable estimates on the total prevalence of chronic wounds for different settings and categories of chronic wounds. Such information is essential for policy and planning purposes as the increasing number of elderly and the prevalence of lifestyle diseases point in the direction of an increased burden. Knowledge about the prevalence and incidence of chronic wounds in relation to population characteristics is important for informing healthcare planning and resource allocation. The objective is to present a transparent process for how to review the existing literature on the prevalence and incidence rates of chronic wounds and resulting implications. Methods/design We will search electronic bibliographic databases (MEDLINE, EMBASE, the EBM Reviews and Cochrane, Cumulative Index to Nursing and allied Health Literature (CINAHL), PsycINFO, Global Health) and reference lists of included articles. Two investigators will independently screen titles and abstracts and select studies involving adults with chronic wounds. These investigators will also independently extract data using a pre-designed data extraction form that will cover information on demographics, diagnostics including disease prevalence, medical history, hospital and community-based management and outcomes. Subgroup analysis and sensitivity analysis will be performed to address the heterogeneity across studies. Meta-analysis will also be performed if homogeneous group of studies will be found. The collective evidence will be further stratified according to the important background variables if allowed. Discussion This study will describe the available epidemiological evidence and summarise prevalence and incidence rates of chronic wounds and related complications. A better understanding of the relationship between population profile and the prevalence of chronic wounds and related complications will be helpful in the development of guidelines for patient management. Systematic review registration PROSPERO CRD42016037355

Precision mapping of glycans at structural and site-specific level is still one of the most challenging tasks in the glycobiology field. Here, we describe a modularization strategy for de novo interpretation of N-glycan structures on intact glycopeptides using tandem mass spectrometry. An algorithm named StrucGP is also developed to automate the interpretation process for large-scale analysis. By dividing an N-glycan into three modules and identifying each module using distinct patterns of Y ions or a combination of distinguishable B/Y ions, the method enables determination of detailed glycan structures on thousands of glycosites in mouse brain, which comprise four types of core structure and 17 branch structures with three glycan subtypes. Owing to the database-independent glycan mapping strategy, StrucGP also facilitates the identification of rare/new glycan structures. The approach will be greatly beneficial for in-depth structural and functional study of glycoproteins in the biomedical research.StrucGP offers a de novo glycan mapping method to determine detailed N-glycan structures at the site-specific level.

Despite rapid progresses in the genome-editing field, in vivo simultaneous overexpression of multiple genes remains challenging. We generated a transgenic mouse using an improved dCas9 system that enables simultaneous and precise in vivo transcriptional activation of multiple genes and long noncoding RNAs in the nervous system. As proof of concept, we were able to use targeted activation of endogenous neurogenic genes in these transgenic mice to directly and efficiently convert astrocytes into functional neurons in vivo. This system provides a flexible and rapid screening platform for studying complex gene networks and gain-of-function phenotypes in the mammalian brain.

Limited information is available regarding spatiotemporal variations of particles with median aerodynamic diameter < 2.5 μm (PM2.5) at high resolutions, and their relationships with meteorological factors in Beijing, China. This study aimed to detect spatiotemporal change patterns of PM2.5 from August 2013 to July 2014 in Beijing, and to assess the relationship between PM2.5 and meteorological factors. Daily and hourly PM2.5 data from the Beijing Environmental Protection Bureau (BJEPB) were analyzed separately. Ordinary kriging (OK) interpolation, time-series graphs, Spearman correlation coefficient and coefficient of divergence (COD) were used to describe the spatiotemporal variations of PM2.5. The Kruskal-Wallis H test, Bonferroni correction, and Mann-Whitney U test were used to assess differences in PM2.5 levels associated with spatial and temporal factors including season, region, daytime and day of week. Relationships between daily PM2.5 and meteorological variables were analyzed using the generalized additive mixed model (GAMM). Annual mean and median of PM2.5 concentrations were 88.07 μg/m3 and 71.00 μg/m3, respectively, from August 2013 to July 2014. PM2.5 concentration was significantly higher in winter (P < 0.0083) and in the southern part of the city (P < 0.0167). Day to day variation of PM2.5 showed a long-term trend of fluctuations, with 2-6 peaks each month. PM2.5 concentration was significantly higher in the night than day (P < 0.0167). Meteorological factors were associated with daily PM2.5 concentration using the GAMM model (R2 = 0.59, AIC = 7373.84). PM2.5 pollution in Beijing shows strong spatiotemporal variations. Meteorological factors influence the PM2.5 concentration with certain patterns. Generally, prior day wind speed, sunlight hours and precipitation are negatively correlated with PM2.5, whereas relative humidity and air pressure three days earlier are positively correlated with PM2.5.

As a fundamental mechanism of post-transcriptional epigenetic regulation, RNA modifications have attracted increasing research interest in recent years. N6-methyladenosine (m 6 A), the most abundant internal RNA modification, plays a crucial role in regulating multiple stages of mRNA metabolism including processing, transport, translation, and degradation. The differentiation of bovine preadipocytes into mature adipocytes is regulated at multiple levels including transcriptional, post-transcription, translation, and post-translation levels. However, the precise regulatory mechanism of m 6 A modification during bovine adipogenesis remains poorly understood. In this study, we conducted MeRIP-seq and RNA-seq analyses across three stages of bovine preadipocyte differentiation—pre-differentiation (day 0, D0), early differentiation (day 2, D2), and late differentiation (day 8, D8)—to characterize the dynamic landscape of m⁶A methylation during adipogenesis. The analysis identified 400 genes exhibiting significant changes in both m⁶A modification levels and mRNA expression during the differentiation phase (D0–D2), while 592 genes exhibited such changes during the lipid accumulation phase (D2–D8). These results indicate that m⁶A modification likely contributes to adipogenesis by modulating the expression of these genes. To further investigate the functional relevance of the m⁶A machinery, we knocked down the m⁶A writer METTL14, a core component of the methyltransferase complex, and performed RNA-seq analysis. Integrative analysis of the differentially expressed genes (DEGs) with the stage-specific expression profile revealed a set of candidate genes potentially regulated by METTL14 that participate in early preadipocyte differentiation and late-stage lipid accumulation. These findings provide new evidence for the molecular mechanisms underlying m⁶A modification during bovine adipogenesis and highlight a functional contribution of the m⁶A writer METTL14.

The digital creative industry (DCI) has become an integral part of China’s strategic emerging industries. This paper, utilizing county-level data from Chinese digital creative enterprises in 2022, examines the locational factors influencing the spatial distribution of China’s DCI through Principal Component Analysis and Multiple Linear Regression Analysis. The findings indicate that technological innovation and the level of economic development universally and dominantly influence the agglomeration of all DCI sub-sectors. Service-oriented digital creative enterprises are more likely to cluster in areas with abundant cultural resources and public facilities, with government policies and financial subsidies playing a significant role. In contrast, digital creative equipment manufacturing companies are more likely to locate in proximity to market demand and related industries.

Using Global Precipitation Climatology Project daily rainfall and ERA interim reanalysis data, we investigate the distinct characteristic of quasi-biweekly variation (QBV: 12–20 days) over East Asia (EA) during early (June 10–July 20) and late (July 21–August 31) summer. The QBV maximum variance is found over the core region of EA (30°–40°N, 110°–130°E), which includes eastern China (lower reaches of the Yellow, Huaihe, and Yangtze rivers) and the Korean Peninsula. At both its peak wet and dry phases, QBV over the core region has a baroclinic structure, but with different spatial distributions, different lower-level prevalent wind anomalies, and different upper-level major circulation anomalies in the two subseasons. Meanwhile, the two subseasons have different propagating tracks prior to reaching the peak phase, and different precursors associated with the local genesis of QBV. Furthermore, during the transition from the peak dry to peak wet phase of QBV, the major monsoon circulations have different behaviors that tropical monsoon trough extends eastward in early summer but retreats westward in late summer and the South Asia high (SAH) and western North Pacific (WNP) subtropical high move toward (away from) each other in early (late) summer. The abrupt change of mean state in mid to late July, which includes the northward migration of westerly jet, SAH and WNP, and the weakening and broken of westerly jet, is considered the root cause of the change in behavior of QBV. Finally, we indicate that the tropical monsoon trough and midlatitude westerly jet are possible sources of QBV over subtropical EA in both subseasons and provide useful guidance for 2–3 week predictions over EA.

There has long been a certain enterprise that leads the line products of lead and zinc, which is facing inequality problems that seriously affect economic benefits. This study uses a confocal microscope, scanning electron microscope, electron probe, and chemical constant volume method to study the causes of the problem and the mechanism of the main influence factors. The results show that the surface roughness of hot rolled acid-washed substrate and the distribution of carbide in steel are the main factors that cause the zinc flower inhomogeneity. The increase of the nucleation point in the cooling process of the zinc pans with the roughness of the base plate. When the distribution of carbides along the ferrite grain boundary in steel occurs, the formation of Fe2Al5 is restricted. This results in a thinner inhibition layer on the substrate surface, leading to less consumption of aluminum. Consequently, the zinc layer nearby retains more aluminum content. Steel and carbide precipitation occurs in the intercrystalline structure, which inhibits the increase in the thickness of the strip’s surface layer. This leads to a higher consumption of aluminum, resulting in a lower zinc layer with reduced aluminum content. As a consequence, the liquid zinc forms droplets, allowing lead (Pb) and antimony (Sb) in the liquid zinc enough time for dendritic segregation to take place. This process increases dendrite growth and results in the formation of larger zinc grains.