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Reportedly, 300 million people worldwide are affected by the consumption of arsenic contaminated groundwater. India prominently figures amongst them and the state of Bihar has shown an upsurge in cases affected by arsenic poisoning. Escalated arsenic content in blood, leaves 1 in every 100 human being highly vulnerable to being affected by the disease. Uncontrolled intake may lead to skin, kidney, liver, bladder, or lung related cancer but even indirect forms of cancer are showing up on a regular basis with abnormal arsenic levels as the probable cause. But despite the apparent relation, the etiology has not been understood clearly. Blood samples of 2000 confirmed cancer patients were collected from pathology department of our institute. For cross-sectional design, 200 blood samples of subjects free from cancer from arsenic free pockets of Patna urban agglomeration, were collected. Blood arsenic levels in carcinoma patients as compared to sarcomas, lymphomas and leukemia were found to be higher. The geospatial map correlates the blood arsenic with cancer types and the demographic area of Gangetic plains. Most of the cancer patients with high blood arsenic concentration were from the districts near the river Ganges. The raised blood arsenic concentration in the 2000 cancer patients strongly correlates the relationship of arsenic with cancer especially the carcinoma type which is more vulnerable. The average arsenic concentration in blood of the cancer patients in the Gangetic plains denotes the significant role of arsenic which is present in endemic proportions. Thus, the study significantly correlates and advocates a strong relation of the deleterious element with the disease. It also underlines the need to address the problem by deciphering the root cause of the elevated cancer incidences in the Gangetic basin of Bihar and its association with arsenic poisoning.

In recent times Gallbladder cancer (GBC) incidences increased many folds in India and are being reported from arsenic hotspots identified in Bihar. The study aims to establish association between arsenic exposure and gallbladder carcinogenesis. In the present study, n = 200 were control volunteers and n = 152 confirmed gallbladder cancer cases. The studied GBC patient’s biological samples-gallbladder tissue, gallbladder stone, bile, blood and hair samples were collected for arsenic estimation. Moreover, n = 512 gallbladder cancer patients blood samples were also evaluated for the presence of arsenic to understand exposure level in the population. A significantly high arsenic concentration ( p  < 0.05) was detected in the blood samples with maximum concentration 389 µg/L in GBC cases in comparison to control. Similarly, in the gallbladder cancer patients, there was significantly high arsenic concentration observed in gallbladder tissue with highest concentration of 2166 µg/kg, in gallbladder stones 635 µg/kg, in bile samples 483 µg/L and in hair samples 6980 µg/kg respectively. Moreover, the n = 512 gallbladder cancer patient’s blood samples study revealed very significant arsenic concentration in the population of Bihar with maximum arsenic concentration as 746 µg/L. The raised arsenic concentration in the gallbladder cancer patients’ biological samples—gallbladder tissue, gallbladder stone, bile, blood, and hair samples was significantly very high in the arsenic exposed area. The study denotes that the gallbladder disease burden is very high in the arsenic exposed area of Bihar. The findings do provide a strong link between arsenic contamination and increased gallbladder carcinogenesis.

Groundwater poisoning by heavy metals has caused serious health hazards in the exposed population globally. Manganese (Mn) poisoning causing human health hazards is very meagerly reported worldwide. The present research elucidates for the first time the catastrophic effect of manganese causing cancer in the Gangetic plains of Bihar (India). The blood samples of n  = 1146 cancer patients were voluntarily obtained for the study, after their consent. Their household water samples were also collected for the study. All the samples were analysed for Mn contamination by Atomic Absorption Spectrophotometer. The study indicates high Mn contamination in the cancer patient blood samples with highest content as 6022 µg/L. Moreover, the cancer patient’s household handpump water samples also contained elevated Mn contamination. The correlation coefficient study finds significant association between Mn contamination in blood of cancer patients and their handpump water. The carcinoma group of cancer patients mostly in Stage III & IV had significant Mn contamination in their blood (above WHO/BIS permissible limit). The geospatial study depicts Mn contamination in handpump water in the state of Bihar in correlation with cancer patient’s blood samples. This novel finding is being reported in India for the first time, which correlates cancer with handpump drinking water. The long-term Mn exposure could be one of the causative agents for elevating cancer incidences. However, other confounding risk factors cannot be denied.

Mercury is a persistent, bio-accumulative, and hazardous contaminant. When released into the environment, it accumulates in water sediments, converting it to poisonous methylmercury that enters the food chain. The present study was carried out in habitations from the 11 districts of Bihar (India). In the study, n  = 224 lactating women and their infants n  = 172 infants participated. After obtaining the written informed consent, their breast milk, urine, and blood were collected for mercury estimation. The breastmilk content was measured in n  = 181 subjects, in which 74% women had their breastmilk higher than the WHO permissible limit (< 1.7 μg/L), while 26% of the women had their breast milk below the permissible limit. The blood mercury content showed that 19% subjects had mercury content above the permissible limit [20 μg/L]. In urine mercury estimation, 49% women had mercury content above the permissible limit [10 μg/L], while, 51% women had the mercury content below the permissible limit. In the child’s urine, 54% infants had their mercury content in urine above the permissible limit [10 μg/L] while 46% infants had content below the permissible limit. The study indicates that 20% of infants had the complete accumulation of mercury in their body which is highly toxic for them. However, the mercury content in the food (wheat) had the contamination with in the permissible limit [100 μg/kg]. There was a significant correlation found between the breastmilk and child’s urine and mother’s urine. The HQ study also correlates the mercury poisoning effect with 100% of the mother’s and 66% of the infants exceeding the limit of non-carcinogenic risk. The Monte Carlo and multivariate study correlates the high health risk in the studied population due to mercury poisoning. The entire study concludes that population inhabitation in the Gangetic plains of Bihar are exposed to mercury poisoning which may be due to geogenic or anthropogenic sources. But, the levels of mercury contamination above the permissible limit could lead to neurogenerative changes in the lactating mothers and their infants. To control the present problem medical intervention is immediately required.

The present study was carried out in the village Kaliprasad of Bhagalpur district of Bihar to know the arsenic exposure effect in the exposed population. A total of n = 102 households were studied, and their water and biological samples such as urine and hair were collected and analyzed in a graphite furnace atomic absorption spectrophotometer (GF-AAS). The assessment of arsenic-exposed village population reveals that the villagers were suffering from serious health-related problems such as skin manifestations (hyperkeratosis and melanosis in their palm and soles), breathlessness, general body weakness, mental disorders, diabetes, hypertension (raised blood pressure), hormonal imbalance, neurological disorders, and few cancer cases. About 77% of household hand pump water had arsenic level more than the WHO recommended level of 10 µg/L, with highest level of 523 µg/L. Moreover, in 60% individual’s urine samples, arsenic concentration was very high with maximum 374 µg/L while in hair 64% individuals had arsenic concentration above the permissible limit with maximum arsenic concentration of 11,398 µg/kg. The hazard quotient (HQ) was also calculated to know the arsenic risk percentage in children as 87.11%, in females as 83.15%, and in males as 82.27% by groundwater. This has surpassed the threshold value of 1×10-6 for carcinogenic risk (CR) in children, female, and male population group in the village. Hence, the exposed population of Kaliprasad village are at very high risk of the disease burden.

In recent years, groundwater uranium [U 238 ] poisoning has posed serious health hazards in the exposed population. In India, an estimated 151 districts and 18 states are reported with groundwater uranium contamination, and about 1.7% of groundwater sources are affected in the state of Bihar (India). The objective of the study is to evaluate the uranium contamination in the breastmilk of lactating mothers and their breastfed infants. To evaluate the uranium exposure in the infants exposed through their mother’s breastmilk, n  = 40 lactating women were selected randomly from different districts of Bihar. After obtaining the written informed consent, their breast milk was collected and analysed for quantification of U 238 . The infants and their mother’s carcinogenic risk (CR) and hazard quotient (HQ) were also studied to know the potential health hazard effects of uranium. The uranium exposure to the infants through their mother’s breastmilk is at a hazardous level. All the analysed breastmilk samples had U 238 contents, which could pose health impacts to infants. The infants are highly vulnerable to potential non-carcinogenic risk in comparison to their mothers due to the real-time uranium elimination from their bodies. The study reveals that the uranium content in the breast milk was significantly high. The 70% of the infant population had the potential to cause non-carcinogenic health effects. From the studied districts, Katihar breastmilk samples had the highest U 238 content. The exposure of uranium to the infant’s body through breast milk is significantly high, which may impact their health. Furthermore, there is also a need for biomonitoring of U 238 in these regions at a broader level.

Groundwater arsenic poisoning has posed serious health hazards in the exposed population. The objective of the study is to evaluate the arsenic ingestion from breastmilk among pediatric population in Bihar. In the present study, the total women selected were n  = 513. Out of which n  = 378 women after consent provided their breastmilk for the study, n  = 58 subjects were non-lactating but had some type of disease in them and n  = 77 subjects denied for the breastmilk sample. Hence, they were selected for the women health study. In addition, urine samples from n  = 184 infants’ urine were collected for human arsenic exposure study. The study reveals that the arsenic content in the exposed women (in 55%) was significantly high in the breast milk against the WHO permissible limit 0.64 µg/L followed by their urine and blood samples as biological marker. Moreover, the child’s urine also had arsenic content greater than the permissible limit (< 50 µg/L) in 67% of the studied children from the arsenic exposed regions. Concerningly, the rate at which arsenic is eliminated from an infant’s body via urine in real time was only 50%. This arsenic exposure to young infants has caused potential risks and future health implications. Moreover, the arsenic content was also very high in the analyzed staple food samples such as rice, wheat and potato which is the major cause for arsenic contamination in breastmilk. The study advocates for prompt action to address the issue and implement stringent legislative measures in order to mitigate and eradicate this pressing problem that has implications for future generations.

Fast growing arsenic menace is causing serious health hazards in Bihar, India, with an estimated 10 million people at risk. The exposed population is often unaware of the problem, which only amplifies the burden of arsenic health effects. In the present study, we have assessed the current situation of arsenic exposure in Chapar village of Samastipur district, Bihar. The health of the inhabitants was assessed and correlated with (1) arsenic concentrations in the groundwater of individual wells and (2) arsenic concentration found in their hair and urine. Altogether, 113 inhabitants were assessed, and 113 hair, urine and groundwater samples were collected. The health study reveals that the exposure to arsenic has caused serious health hazard amongst the exposed population with pronounced skin manifestations, loss of appetite, anaemia, constipation, diarrhoea, general body weakness, raised blood pressure, breathlessness, diabetes, mental disabilities, diabetes, lumps in the body and few cancer incidences. It was found that 52% of the total collected groundwater samples had arsenic levels higher than the WHO limit of 10 µg/l (with a maximum arsenic concentration of 1212 µg/l) and the reduced arsenite was the predominant form in samples tested for speciation ( N  = 19). In the case of hair samples, 29% of the samples had arsenic concentrations higher than the permissible limit of 0.2 mg/kg, with a maximum arsenic concentration of 46 µg/l, while in 20% exposed population, there was significant arsenic contamination in urine samples > 50 µg/l. In Chapar village, the probability of carcinogenic-related risk in the exposed population consuming arsenic contaminated water is 100% for children, 99.1% for females and 97.3% for male subjects. The assessment report shared to the government enabled the village population to receive two arsenic filter units. These units are currently operational and catering 250 households providing arsenic-free water through piped water scheme. This study therefore identified a significant solution for this arsenic-exposed population.

Aflatoxin (AF) and Ochratoxin A (OTA) are potent dietary contaminant of agrarian products, leading to hepatotoxicity and nephrotoxicity. The current study aims to evaluate the sub-acute toxicity impact of AF and OTA on Charles Foster rats. A total of n = 40, adult male rats were randomly divided into ten groups; a control group, a 30 days, 45 days and 60 days treated groups of orally administered 0.9 mg/kg body wt./day of AF, 2.75 mg/kg body wt/day of OTA and (0.45 + 1.375) mg/kg body weight/day of AF + OTA. There were significant alterations observed in the blood chemistry with normocytic anemia in AF and OTA treated groups. There was significant increase in liver function tests such as AST and ALP with significant increase in the Kupffer cells in the liver of AF and OTA treated groups. In AF + OTA significant elevation of AST only observed. There were significant histopathological changes observed in liver tissue in all the treated groups which are in correlation with biochemical analysis. The significant elevation in uric acid and creatinine levels is in correlation with nephrotoxicity in OTA treated group. There was initial significant elevation of creatinine observed only in 45 days treated group of AF + OTA. The histopathological analysis of AF + OTA shows chronic toxicity in kidney tissue. Taken together, this study indicates that AF induces significant biochemical and histological alterations in liver and OTA in kidney which significantly correlates with impaired qualitative and quantitative analysis. The biochemical changes are not in correlation with chronic nephrotoxicity observed in AF + OTA groups and hence it might act as silent toxicity.

Background Toxicity by pesticide has become a global health issue and leaves a harmful impact on human health via various ways. The people exposed to pesticides in the rural population get affected by the harmful effects of it as they enter the human body system through skin, inhalation, oral administration, food chain and many more ways. The present work is designed to study the toxic effect of endosulfan in male (n=30) and female (n=30) Swiss albino mice. Endosulfan was administered by oral gavage (oral administration) method, at the dose of 3.5 mg/Kg body weight daily for period of 3 weeks, 5 weeks and 7 weeks. After the completion of the treatment, the mice were sacrificed and their ovary and testis tissues were dissected out to check the degeneration. The blood was collected for karyotyping, biochemical and hormonal analysis of pesticide induced genotoxicity. After 7 weeks of administration with Endosulfan, various abnormalities were observed in male and female mice. Results Treatment with endosulfan at the dose of 3.5 mg/Kg body weight caused a higher degree of degeneration in the reproductive organ of Swiss albino mice . Treatment by this pesticide generated degeneration in long duration of dosage for 3,5 and 7 weeks. Ovaries of endosulfan administered groups showed degenerated germinal epithelium, Graffian follicles and corpus luteum. In testis of endosulfan treated mice, microscopic examination showed that there is significant damage and reduction in the tissue of seminiferous tubules and primordial germ cells. High degree of degeneration caused the disarrangement and deformation of spermatogonia with the decrease in the number of Sertoli cells. Biochemical and hormonal properties was also affected by endosulfan treatment. There was significant 5 folds decrease in the testosterone value of endosulfan in 7 weeks treated mice in comparison to control (p < 0.0001) and similarly there was significant elevation in the estrogen levels found in 7th week endosulfan treated mice. It also influenced the level of free radicals as there was significant decrease (p < 0.0001) in the value in catalase levels in 7 weeks endosulfan treated male and female mice, while significant (p < 0.0001) increase in the values of lipid peroxidation levels as 8 folds and 10 folds in 7 weeks endosulfan treated male and female Swiss albino mice respectively. This study hence speculates that the endosulfan exposed population are at the risk of reproductive health hazards. Conclusions The present study thus concludes that, endosulfan after 7 weeks of exposure caused significant reproductive damage to both male and female Swiss albino mice groups. Moreover, the karyotyping study also correlated the genotoxic damage in the mice.